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Shimadzu Corporation chromatographic conditions chromatography
Chromatographic Conditions Chromatography, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chromatographic conditions chromatography/product/Shimadzu Corporation
Average 86 stars, based on 1 article reviews
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chromatographic conditions chromatography - by Bioz Stars, 2021-07
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Fractionation:

Article Title: Thiocarbamates from Moringa oleifera Seeds Bioactive against Virulent and Multidrug-Resistant Vibrio Species
Article Snippet: The bioactive fraction was subjected to chromatographic fractionation by High-Performance Liquid Chromatography (HPLC) in order to isolate its active principles. .. Chromatographic Fractionation of the Ethyl Acetate Fraction (MOS-ESA) by High-Performance Liquid Chromatography (HPLC) and Isolation of Active Substances Part of the MOE-ESA active fraction (285 mg) was analyzed by HPLC in a chromatograph Shimadzu® (UFLC model) equipped with a UV-Vis detector with diode array (model SPD-M20A). .. Separation was performed in reverse phase conditions in semipreparative column (C-18.5 μ m), with isocratic elution using MeOH/H2 O (1 : 1) with a 4.72 mL min−1 flow.

High Performance Liquid Chromatography:

Article Title: Thiocarbamates from Moringa oleifera Seeds Bioactive against Virulent and Multidrug-Resistant Vibrio Species
Article Snippet: The bioactive fraction was subjected to chromatographic fractionation by High-Performance Liquid Chromatography (HPLC) in order to isolate its active principles. .. Chromatographic Fractionation of the Ethyl Acetate Fraction (MOS-ESA) by High-Performance Liquid Chromatography (HPLC) and Isolation of Active Substances Part of the MOE-ESA active fraction (285 mg) was analyzed by HPLC in a chromatograph Shimadzu® (UFLC model) equipped with a UV-Vis detector with diode array (model SPD-M20A). .. Separation was performed in reverse phase conditions in semipreparative column (C-18.5 μ m), with isocratic elution using MeOH/H2 O (1 : 1) with a 4.72 mL min−1 flow.

Article Title: Solanum paranense Extracts and Solanine Present Anti-Inflammatory Activity in an Acute Skin Inflammation Model in Mice
Article Snippet: .. HPLC Analysis of Phenolic Compounds on S. paranense Extract The phenolic compounds analysis was performed by high performance liquid chromatography (HPLC), through the method described by Zadra et al. [ ], slightly modified in a Shimadzu Prominence system (Kyoto, Japan) equipped with a SIL-20A autosampler, equipped with Shimadzu LC-20 at reciprocating pumps connected to the degasser DGU 20A5 with integrator CBM 20A, UV–VIS detector DAD SPD-M20A, and Software LC Solution 1.22 SP1. ..

Article Title: Intestinal Anti-Inflammatory Activity of Baccharis dracunculifolia in the Trinitrobenzenesulphonic Acid Model of Rat Colitis
Article Snippet: .. Phytochemical Analysis of Plant Extract by HPLC The ethyl acetate extract of the aerial parts from B. dracunculifolia was submitted to HPLC analysis using the following equipment and conditions: a Shimadzu high performance liquid-chromatograph (SCL-10Avp system controller, three LC-10AD pumps, SPD-M10Avp photodiode array detector and Shimadzu Class-VP 5.02 software). ..

Article Title: Anti-Acne Activity of Italian Medicinal Plants Used for Skin Infection
Article Snippet: ACS grade tannic acid from Sigma Aldrich (St. Louis, MO) was characterized prior to use by HPLC-PDA analysis. .. Liquid chromatography-Fourier transform mass spectrometry (LC-FTMS) was performed on the MeOH extract of Hapalopilus rutilans using a Shimadzu SIL-ACHT (Kyoto, Japan) and Dionex 3600SD HPLC pump (Sunnyvale, CA) with the above described chromatographic conditions. .. The data was acquired in MS1 mode scanning from m/z 150 to 1500 on a Thermo Scientific LTQ-FT Ultra MS (Waltham, MA) in negative ESI mode and processed with Thermo Scientific Xcalibur 2.2 SP1.48 software (San Jose, CA).

Article Title: A rapid method for the determination of dopamine in porcine muscle by pre-column derivatization and HPLC with fluorescence detection
Article Snippet: After derivatization, dopamine was transformed into substituted isoindole, which allows fluorescence detection, the reaction mechanism is shown in . .. 2.3 HPLC-FLD and derivatization conditions For chromatographic separation, an LC-10Avp (Shimadzu, Japan) chromatography system was used. ..

Article Title: Preparation and Evaluation of Resveratrol-Loaded Composite Nanoparticles Using a Supercritical Fluid Technology for Enhanced Oral and Skin Delivery
Article Snippet: .. The concentration of resveratrol was then determined using a Shimadzu HPLC system (Shimadzu, Tokyo, Japan) consisting of an SPD-20A ultraviolet–visible (UV/VIS) detector, CBM-20A communications bus module, SIL-20AC autosampler, LC-20AT liquid chromatograph, DGU-20A 5R degassing unit, and a C18 analytical column (4.6 × 150 mm, 5 μm, Shiseido, Tokyo, Japan). .. HPLC analysis conditions were as follows: a 40% acetonitrile in water mobile phase, 0.8 mL/min flow rate, 30 °C column temperature, 10 μL injection volume, and UV detector wavelength of 303 nm.

Isolation:

Article Title: Thiocarbamates from Moringa oleifera Seeds Bioactive against Virulent and Multidrug-Resistant Vibrio Species
Article Snippet: The bioactive fraction was subjected to chromatographic fractionation by High-Performance Liquid Chromatography (HPLC) in order to isolate its active principles. .. Chromatographic Fractionation of the Ethyl Acetate Fraction (MOS-ESA) by High-Performance Liquid Chromatography (HPLC) and Isolation of Active Substances Part of the MOE-ESA active fraction (285 mg) was analyzed by HPLC in a chromatograph Shimadzu® (UFLC model) equipped with a UV-Vis detector with diode array (model SPD-M20A). .. Separation was performed in reverse phase conditions in semipreparative column (C-18.5 μ m), with isocratic elution using MeOH/H2 O (1 : 1) with a 4.72 mL min−1 flow.

Modification:

Article Title: Solanum paranense Extracts and Solanine Present Anti-Inflammatory Activity in an Acute Skin Inflammation Model in Mice
Article Snippet: .. HPLC Analysis of Phenolic Compounds on S. paranense Extract The phenolic compounds analysis was performed by high performance liquid chromatography (HPLC), through the method described by Zadra et al. [ ], slightly modified in a Shimadzu Prominence system (Kyoto, Japan) equipped with a SIL-20A autosampler, equipped with Shimadzu LC-20 at reciprocating pumps connected to the degasser DGU 20A5 with integrator CBM 20A, UV–VIS detector DAD SPD-M20A, and Software LC Solution 1.22 SP1. ..

Software:

Article Title: Solanum paranense Extracts and Solanine Present Anti-Inflammatory Activity in an Acute Skin Inflammation Model in Mice
Article Snippet: .. HPLC Analysis of Phenolic Compounds on S. paranense Extract The phenolic compounds analysis was performed by high performance liquid chromatography (HPLC), through the method described by Zadra et al. [ ], slightly modified in a Shimadzu Prominence system (Kyoto, Japan) equipped with a SIL-20A autosampler, equipped with Shimadzu LC-20 at reciprocating pumps connected to the degasser DGU 20A5 with integrator CBM 20A, UV–VIS detector DAD SPD-M20A, and Software LC Solution 1.22 SP1. ..

Article Title: Intestinal Anti-Inflammatory Activity of Baccharis dracunculifolia in the Trinitrobenzenesulphonic Acid Model of Rat Colitis
Article Snippet: .. Phytochemical Analysis of Plant Extract by HPLC The ethyl acetate extract of the aerial parts from B. dracunculifolia was submitted to HPLC analysis using the following equipment and conditions: a Shimadzu high performance liquid-chromatograph (SCL-10Avp system controller, three LC-10AD pumps, SPD-M10Avp photodiode array detector and Shimadzu Class-VP 5.02 software). ..

Liquid Chromatography:

Article Title: Quantification of Sterol and Triterpenol Biomarkers in Sediments of the Cananéia-Iguape Estuarine-Lagoonal System (Brazil) by UHPLC-MS/MS
Article Snippet: .. Instrumentation and Chromatographic Conditions Analysis of the sterols and triterpenols in the sedimentary OM extracts was conducted using an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) system model LCMS-8040 (Shimadzu, Kyoto, Japan). .. The mass spectrometer system consists of a triple quadrupole equipped with an atmospheric pressure chemical ionization (APCI) probe.

Mass Spectrometry:

Article Title: Quantification of Sterol and Triterpenol Biomarkers in Sediments of the Cananéia-Iguape Estuarine-Lagoonal System (Brazil) by UHPLC-MS/MS
Article Snippet: .. Instrumentation and Chromatographic Conditions Analysis of the sterols and triterpenols in the sedimentary OM extracts was conducted using an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) system model LCMS-8040 (Shimadzu, Kyoto, Japan). .. The mass spectrometer system consists of a triple quadrupole equipped with an atmospheric pressure chemical ionization (APCI) probe.

Article Title: An alternative method for production of microalgal biodiesel using novel Bacillus lipase
Article Snippet: .. Gas chromatographic analysis of fatty acid methyl esters Fatty acid methyl ester composition of biodiesel produced from N. oculata oil was analyzed by gas chromatography–mass spectrometry (GC–MS-QP 2010, Shimadzu) equipped with VF-5 MS capillary column (nonpolar, 30 mm length, 0.25 mm diameter and 0.25 µm film thickness). .. The column temperature of each run was started at 70 °C for 3 min, then raised to 300 °C and maintained at 300 °C for 9 min. GC conditions were: column oven temperature, 70 °C; injector temperature, 240 °C; injection mode, split; split ratio, 10; flow control mode, linear velocity; column flow, 1.51 ml/min; carrier gas, helium (99.9995 % purity); and injection volume, 1 µl.

Article Title: Anti-Acne Activity of Italian Medicinal Plants Used for Skin Infection
Article Snippet: ACS grade tannic acid from Sigma Aldrich (St. Louis, MO) was characterized prior to use by HPLC-PDA analysis. .. Liquid chromatography-Fourier transform mass spectrometry (LC-FTMS) was performed on the MeOH extract of Hapalopilus rutilans using a Shimadzu SIL-ACHT (Kyoto, Japan) and Dionex 3600SD HPLC pump (Sunnyvale, CA) with the above described chromatographic conditions. .. The data was acquired in MS1 mode scanning from m/z 150 to 1500 on a Thermo Scientific LTQ-FT Ultra MS (Waltham, MA) in negative ESI mode and processed with Thermo Scientific Xcalibur 2.2 SP1.48 software (San Jose, CA).

Liquid Chromatography with Mass Spectroscopy:

Article Title: Quantification of Sterol and Triterpenol Biomarkers in Sediments of the Cananéia-Iguape Estuarine-Lagoonal System (Brazil) by UHPLC-MS/MS
Article Snippet: .. Instrumentation and Chromatographic Conditions Analysis of the sterols and triterpenols in the sedimentary OM extracts was conducted using an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) system model LCMS-8040 (Shimadzu, Kyoto, Japan). .. The mass spectrometer system consists of a triple quadrupole equipped with an atmospheric pressure chemical ionization (APCI) probe.

Produced:

Article Title: An alternative method for production of microalgal biodiesel using novel Bacillus lipase
Article Snippet: .. Gas chromatographic analysis of fatty acid methyl esters Fatty acid methyl ester composition of biodiesel produced from N. oculata oil was analyzed by gas chromatography–mass spectrometry (GC–MS-QP 2010, Shimadzu) equipped with VF-5 MS capillary column (nonpolar, 30 mm length, 0.25 mm diameter and 0.25 µm film thickness). .. The column temperature of each run was started at 70 °C for 3 min, then raised to 300 °C and maintained at 300 °C for 9 min. GC conditions were: column oven temperature, 70 °C; injector temperature, 240 °C; injection mode, split; split ratio, 10; flow control mode, linear velocity; column flow, 1.51 ml/min; carrier gas, helium (99.9995 % purity); and injection volume, 1 µl.

Gas Chromatography-Mass Spectrometry:

Article Title: An alternative method for production of microalgal biodiesel using novel Bacillus lipase
Article Snippet: .. Gas chromatographic analysis of fatty acid methyl esters Fatty acid methyl ester composition of biodiesel produced from N. oculata oil was analyzed by gas chromatography–mass spectrometry (GC–MS-QP 2010, Shimadzu) equipped with VF-5 MS capillary column (nonpolar, 30 mm length, 0.25 mm diameter and 0.25 µm film thickness). .. The column temperature of each run was started at 70 °C for 3 min, then raised to 300 °C and maintained at 300 °C for 9 min. GC conditions were: column oven temperature, 70 °C; injector temperature, 240 °C; injection mode, split; split ratio, 10; flow control mode, linear velocity; column flow, 1.51 ml/min; carrier gas, helium (99.9995 % purity); and injection volume, 1 µl.

Chromatography:

Article Title: Anti-Acne Activity of Italian Medicinal Plants Used for Skin Infection
Article Snippet: ACS grade tannic acid from Sigma Aldrich (St. Louis, MO) was characterized prior to use by HPLC-PDA analysis. .. Liquid chromatography-Fourier transform mass spectrometry (LC-FTMS) was performed on the MeOH extract of Hapalopilus rutilans using a Shimadzu SIL-ACHT (Kyoto, Japan) and Dionex 3600SD HPLC pump (Sunnyvale, CA) with the above described chromatographic conditions. .. The data was acquired in MS1 mode scanning from m/z 150 to 1500 on a Thermo Scientific LTQ-FT Ultra MS (Waltham, MA) in negative ESI mode and processed with Thermo Scientific Xcalibur 2.2 SP1.48 software (San Jose, CA).

Article Title: A rapid method for the determination of dopamine in porcine muscle by pre-column derivatization and HPLC with fluorescence detection
Article Snippet: After derivatization, dopamine was transformed into substituted isoindole, which allows fluorescence detection, the reaction mechanism is shown in . .. 2.3 HPLC-FLD and derivatization conditions For chromatographic separation, an LC-10Avp (Shimadzu, Japan) chromatography system was used. ..

Concentration Assay:

Article Title: Preparation and Evaluation of Resveratrol-Loaded Composite Nanoparticles Using a Supercritical Fluid Technology for Enhanced Oral and Skin Delivery
Article Snippet: .. The concentration of resveratrol was then determined using a Shimadzu HPLC system (Shimadzu, Tokyo, Japan) consisting of an SPD-20A ultraviolet–visible (UV/VIS) detector, CBM-20A communications bus module, SIL-20AC autosampler, LC-20AT liquid chromatograph, DGU-20A 5R degassing unit, and a C18 analytical column (4.6 × 150 mm, 5 μm, Shiseido, Tokyo, Japan). .. HPLC analysis conditions were as follows: a 40% acetonitrile in water mobile phase, 0.8 mL/min flow rate, 30 °C column temperature, 10 μL injection volume, and UV detector wavelength of 303 nm.

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    Shimadzu Corporation hplc q tof ms ms analysis conditions chromatographic
    TIC of Schisandra extracts by <t>HPLC-Q-TOF-MS-MS.</t> (A) Positive ion mode MS spectra of SchisandraSchisandra chinensis; (B) Negative ion mode MS spectra of SchisandraSchisandra chinensis.
    Hplc Q Tof Ms Ms Analysis Conditions Chromatographic, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc q tof ms ms analysis conditions chromatographic/product/Shimadzu Corporation
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc q tof ms ms analysis conditions chromatographic - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

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    Shimadzu Corporation lc ms ms system
    TIC of Schisandra extracts by <t>HPLC-Q-TOF-MS-MS.</t> (A) Positive ion mode MS spectra of SchisandraSchisandra chinensis; (B) Negative ion mode MS spectra of SchisandraSchisandra chinensis.
    Lc Ms Ms System, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc ms ms system/product/Shimadzu Corporation
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lc ms ms system - by Bioz Stars, 2021-07
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    99
    Shimadzu Corporation lc 20a prominence uflc xr ultra high performance liquid chromatography system
    TIC of Schisandra extracts by <t>HPLC-Q-TOF-MS-MS.</t> (A) Positive ion mode MS spectra of SchisandraSchisandra chinensis; (B) Negative ion mode MS spectra of SchisandraSchisandra chinensis.
    Lc 20a Prominence Uflc Xr Ultra High Performance Liquid Chromatography System, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc 20a prominence uflc xr ultra high performance liquid chromatography system/product/Shimadzu Corporation
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lc 20a prominence uflc xr ultra high performance liquid chromatography system - by Bioz Stars, 2021-07
    99/100 stars
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    TIC of Schisandra extracts by HPLC-Q-TOF-MS-MS. (A) Positive ion mode MS spectra of SchisandraSchisandra chinensis; (B) Negative ion mode MS spectra of SchisandraSchisandra chinensis.

    Journal: Frontiers in Pharmacology

    Article Title: Analysis of Effect of Schisandra in the Treatment of Myocardial Infarction Based on Three-Mode Gene Ontology Network

    doi: 10.3389/fphar.2019.00232

    Figure Lengend Snippet: TIC of Schisandra extracts by HPLC-Q-TOF-MS-MS. (A) Positive ion mode MS spectra of SchisandraSchisandra chinensis; (B) Negative ion mode MS spectra of SchisandraSchisandra chinensis.

    Article Snippet: HPLC-Q-TOF-MS-MS Analysis Conditions Chromatographic experiments were conducted on a Shimadzu Shimadzu (Kyoto, Japan) LC-2010 series.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    The Schisandra three-mode GOI network analysis workflow. (A) The components in the Schisandra extract were identified through HPLC-Q-TOF-MS-MS. Target proteins relevant to component (T C ) were then obtained through PharmMapper. AMI related differential expressed genes (T AMI ) and pharmacological effect related proteins (T E ) were screened through data mining from three different databases (B) . After target data were enriched through the GO annotation database, the relationship between the component and pharmacological effects were connected through GO terms, and the “component–gene ontology–effect” three-mode network was constructed. The relation score between component and effect was calculated using a maximum flow algorithm. In the end, the prediction results were validated through pharmacological experiments (C) , with the corresponding drug given to mice one day after ligation of the left anterior descending.

    Journal: Frontiers in Pharmacology

    Article Title: Analysis of Effect of Schisandra in the Treatment of Myocardial Infarction Based on Three-Mode Gene Ontology Network

    doi: 10.3389/fphar.2019.00232

    Figure Lengend Snippet: The Schisandra three-mode GOI network analysis workflow. (A) The components in the Schisandra extract were identified through HPLC-Q-TOF-MS-MS. Target proteins relevant to component (T C ) were then obtained through PharmMapper. AMI related differential expressed genes (T AMI ) and pharmacological effect related proteins (T E ) were screened through data mining from three different databases (B) . After target data were enriched through the GO annotation database, the relationship between the component and pharmacological effects were connected through GO terms, and the “component–gene ontology–effect” three-mode network was constructed. The relation score between component and effect was calculated using a maximum flow algorithm. In the end, the prediction results were validated through pharmacological experiments (C) , with the corresponding drug given to mice one day after ligation of the left anterior descending.

    Article Snippet: HPLC-Q-TOF-MS-MS Analysis Conditions Chromatographic experiments were conducted on a Shimadzu Shimadzu (Kyoto, Japan) LC-2010 series.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Construct, Flow Cytometry, Mouse Assay, Ligation