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Agilent technologies chromatographic conditions chromatography
Chromatographic Conditions Chromatography, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chromatographic conditions chromatography - by Bioz Stars, 2021-07
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Liquid Chromatography with Mass Spectroscopy:

Article Title: Saponin from Periploca forrestii Schltr Mitigates Oxazolone-Induced Atopic Dermatitis via Modulating Macrophage Activation
Article Snippet: .. Chromatographic Conditions The content of periplocin in PFS was analyzed using an Agilent 1290-6495 Accurate-Mass Q-TOF LC/MS system (Agilent Technologies, USA). .. HPLC analysis was carried out on a C18 column (100 × 1.8 mm, i.d 2.1 μ m) at a flow rate of 0.4 mL/min.

High Performance Liquid Chromatography:

Article Title: Gene cloning of a neutral ceramidase from the sphingolipid metabolic pathway based on transcriptome analysis of Amorphophallus muelleri
Article Snippet: The yeasts at different culture time points were solubilized in SDS/PAGE sample buffer, and the cell lysates were isolated as mentioned previously [ ]. .. The homogenates were assayed for the following tests in which D-erythro-C12-NBD-ceramide was used as a substrate and its content was analysed by reverse-phase HPLC (High Performance Liquid Chromatography) with DAD detector (Determination conditions: Sino Chrom C18 reversed-phase column, Agilent 1100 chromatographic system, detector wavelength of 230 nm, column temperature 25 °C, acetonitrile as the mobile phase, sample size 10 μl) and the standard curve was consequently obtained. .. To test the biochemical properties of recombinant AmCDase, , the samples were initialized with the same amount of D-e rythro-C12-NBD-ceramide as a substrate, but the different amounts of recombinant AmCDase that reacted with the substrate were added into these samples.

Article Title: Influence of the Medium Composition and the Culture Conditions on Surfactin Biosynthesis by a Native Bacillus subtilis natto BS19 Strain
Article Snippet: .. The surfactin concentration was determined by high performance liquid chromatography (HPLC) using an Agilent Technologies model 1220 device equipped with a diode detector. .. The chromatographic separation was carried out under the following conditions: Poroshell 120 EC-C18 column (4.6 × 150 mm), mobile phase 80:20 (acetonitrile: 3.8 mM trifluoroacetic acid), 40 °C, detection at 205 nm.

Article Title: Photochemical and Photobiological Properties of Pyridyl-pyrazol(in)e-Based Ruthenium(II) Complexes with Sub-micromolar Cytotoxicity for Phototherapy
Article Snippet: The Prism software package was used to analyze the data. .. 4.2 HPLC Analysis for Purity and Photoejection Products The purity of each of the Ru(II) complexes 1 – 12 and photoejection products were analyzed using an Agilent 1100 series HPLC equipped with a model G1311A quaternary pump, G1315B UV diode array detector (detection wavelength of 280 nm), and ChemStation software version B.01.03. ..

Concentration Assay:

Article Title: Influence of the Medium Composition and the Culture Conditions on Surfactin Biosynthesis by a Native Bacillus subtilis natto BS19 Strain
Article Snippet: .. The surfactin concentration was determined by high performance liquid chromatography (HPLC) using an Agilent Technologies model 1220 device equipped with a diode detector. .. The chromatographic separation was carried out under the following conditions: Poroshell 120 EC-C18 column (4.6 × 150 mm), mobile phase 80:20 (acetonitrile: 3.8 mM trifluoroacetic acid), 40 °C, detection at 205 nm.

Software:

Article Title: Photochemical and Photobiological Properties of Pyridyl-pyrazol(in)e-Based Ruthenium(II) Complexes with Sub-micromolar Cytotoxicity for Phototherapy
Article Snippet: The Prism software package was used to analyze the data. .. 4.2 HPLC Analysis for Purity and Photoejection Products The purity of each of the Ru(II) complexes 1 – 12 and photoejection products were analyzed using an Agilent 1100 series HPLC equipped with a model G1311A quaternary pump, G1315B UV diode array detector (detection wavelength of 280 nm), and ChemStation software version B.01.03. ..

Gas Chromatography-Mass Spectrometry:

Article Title: p-Nitrophenyl esters provide new insights and applications for the thiolase enzyme OleA
Article Snippet: .. 2.6 GC–MS conditions for screening Claisen condensation activity with p -nitrophenyl esters Gas chromatography-mass spectrometry (GC–MS) was carried out on an Agilent 7890a gas chromatograph and an Agilent 5975c mass spectrometer ( Santa Clara, CA ). ..

Activity Assay:

Article Title: p-Nitrophenyl esters provide new insights and applications for the thiolase enzyme OleA
Article Snippet: .. 2.6 GC–MS conditions for screening Claisen condensation activity with p -nitrophenyl esters Gas chromatography-mass spectrometry (GC–MS) was carried out on an Agilent 7890a gas chromatograph and an Agilent 5975c mass spectrometer ( Santa Clara, CA ). ..

Mass Spectrometry:

Article Title: p-Nitrophenyl esters provide new insights and applications for the thiolase enzyme OleA
Article Snippet: .. 2.6 GC–MS conditions for screening Claisen condensation activity with p -nitrophenyl esters Gas chromatography-mass spectrometry (GC–MS) was carried out on an Agilent 7890a gas chromatograph and an Agilent 5975c mass spectrometer ( Santa Clara, CA ). ..

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    Agilent technologies zorbax xdb c18
    The HPLC chromatograms of standard ( a ) and samples. ( b ) LJF sample; ( c ) LF sample; S: secologanic acid. Column: An Agilent <t>ZORBAX</t> <t>XDB</t> <t>C18</t> (4.6 × 250 mm, 5 μm) column; column temperature, 30 °C; mobile phases: 0.1% phosphoric acid solution (A) and acetonitrile (B), using a gradient elution of 5–15% B at 0–20 min, 15–23% B at 20–25 min, 23–95% B at 25–26 min, and 95% B at 26–30 min; flow rate: 1.0 mL/min; detection wavelength: 240 nm; injection volume: 10 µL.
    Zorbax Xdb C18, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zorbax xdb c18/product/Agilent technologies
    Average 97 stars, based on 1 article reviews
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    86
    Agilent technologies hplc dad
    <t>HPLC-DAD</t> chromatogram of five reference compounds in DJS detected at 230 nm (albiflorin, paeoniflorin) and 330 nm (nodakenin, z -ligustilide, and decursin). DJS: Dangguijagyagsan aqueous extract (29.28 mg/mL).
    Hplc Dad, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Agilent technologies hplc q tof ms conditions chromatographic
    Total ion chromatograms (TICs) of rat urine and plasma samples by <t>HPLC/Q-TOF/MS.</t> (a) TIC of the urine sample after oral administration at a single dose of 50 mg/kg BG; (b) TIC of blank urine; (c) TIC of the plasma sample after oral administration at a single dose of 50 mg/kg BG; (d) TIC of blank plasma.
    Hplc Q Tof Ms Conditions Chromatographic, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hplc q tof ms conditions chromatographic - by Bioz Stars, 2021-07
    86/100 stars
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    97
    Agilent technologies 1100 hplc series system
    Total ion chromatograms (TICs) of rat urine and plasma samples by <t>HPLC/Q-TOF/MS.</t> (a) TIC of the urine sample after oral administration at a single dose of 50 mg/kg BG; (b) TIC of blank urine; (c) TIC of the plasma sample after oral administration at a single dose of 50 mg/kg BG; (d) TIC of blank plasma.
    1100 Hplc Series System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1100 hplc series system - by Bioz Stars, 2021-07
    97/100 stars
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    The HPLC chromatograms of standard ( a ) and samples. ( b ) LJF sample; ( c ) LF sample; S: secologanic acid. Column: An Agilent ZORBAX XDB C18 (4.6 × 250 mm, 5 μm) column; column temperature, 30 °C; mobile phases: 0.1% phosphoric acid solution (A) and acetonitrile (B), using a gradient elution of 5–15% B at 0–20 min, 15–23% B at 20–25 min, 23–95% B at 25–26 min, and 95% B at 26–30 min; flow rate: 1.0 mL/min; detection wavelength: 240 nm; injection volume: 10 µL.

    Journal: Molecules

    Article Title: A Simple, Rapid, and Practical Method for Distinguishing Lonicerae Japonicae Flos from Lonicerae Flos

    doi: 10.3390/molecules24193455

    Figure Lengend Snippet: The HPLC chromatograms of standard ( a ) and samples. ( b ) LJF sample; ( c ) LF sample; S: secologanic acid. Column: An Agilent ZORBAX XDB C18 (4.6 × 250 mm, 5 μm) column; column temperature, 30 °C; mobile phases: 0.1% phosphoric acid solution (A) and acetonitrile (B), using a gradient elution of 5–15% B at 0–20 min, 15–23% B at 20–25 min, 23–95% B at 25–26 min, and 95% B at 26–30 min; flow rate: 1.0 mL/min; detection wavelength: 240 nm; injection volume: 10 µL.

    Article Snippet: Chromatographic Conditions An Agilent ZORBAX XDB C18 (4.6 × 250 mm, 5 μm) column was employed, and the column temperature was maintained at 30 °C.

    Techniques: High Performance Liquid Chromatography, Flow Cytometry, Injection

    The overlapping fingerprints of L. japonicae flos (LJF) and LF ( a ) and the UV spectrum of compound S in LJF ( b ). Column: An Agilent ZORBAX XDB C18 (4.6 × 250 mm, 5 μm) column; column temperature, 30 °C; mobile phases: phosphoric acid solution (A) and acetonitrile (B) (5–15% B at 0–20 min, 15–55% B at 20–45 min, and 55–100% B at 45–60 min); flow rate: 1.0 mL/min; detection wavelength: 254 nm; injection volume: 10 µL.

    Journal: Molecules

    Article Title: A Simple, Rapid, and Practical Method for Distinguishing Lonicerae Japonicae Flos from Lonicerae Flos

    doi: 10.3390/molecules24193455

    Figure Lengend Snippet: The overlapping fingerprints of L. japonicae flos (LJF) and LF ( a ) and the UV spectrum of compound S in LJF ( b ). Column: An Agilent ZORBAX XDB C18 (4.6 × 250 mm, 5 μm) column; column temperature, 30 °C; mobile phases: phosphoric acid solution (A) and acetonitrile (B) (5–15% B at 0–20 min, 15–55% B at 20–45 min, and 55–100% B at 45–60 min); flow rate: 1.0 mL/min; detection wavelength: 254 nm; injection volume: 10 µL.

    Article Snippet: Chromatographic Conditions An Agilent ZORBAX XDB C18 (4.6 × 250 mm, 5 μm) column was employed, and the column temperature was maintained at 30 °C.

    Techniques: Flow Cytometry, Injection

    HPLC-DAD chromatogram of five reference compounds in DJS detected at 230 nm (albiflorin, paeoniflorin) and 330 nm (nodakenin, z -ligustilide, and decursin). DJS: Dangguijagyagsan aqueous extract (29.28 mg/mL).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Effects of an Aqueous Extract of Dangguijagyagsan on Serum Lipid Levels and Blood Flow Improvement in Ovariectomized Rats

    doi: 10.1155/2014/497836

    Figure Lengend Snippet: HPLC-DAD chromatogram of five reference compounds in DJS detected at 230 nm (albiflorin, paeoniflorin) and 330 nm (nodakenin, z -ligustilide, and decursin). DJS: Dangguijagyagsan aqueous extract (29.28 mg/mL).

    Article Snippet: Chromatographic Conditions of HPLC-DAD The contents albiflorin, paeoniflorin, z -ligustilide, decursin, and nodakenin in the DJS water extract were analyzed using an HPLC instrument (Agilent Technologies, USA) with a Atlantis dC18 column (4.6 × 250 mm, 5 μ m; Waters, USA).

    Techniques: High Performance Liquid Chromatography

    Total ion chromatograms (TICs) of rat urine and plasma samples by HPLC/Q-TOF/MS. (a) TIC of the urine sample after oral administration at a single dose of 50 mg/kg BG; (b) TIC of blank urine; (c) TIC of the plasma sample after oral administration at a single dose of 50 mg/kg BG; (d) TIC of blank plasma.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats

    doi: 10.1155/2013/473649

    Figure Lengend Snippet: Total ion chromatograms (TICs) of rat urine and plasma samples by HPLC/Q-TOF/MS. (a) TIC of the urine sample after oral administration at a single dose of 50 mg/kg BG; (b) TIC of blank urine; (c) TIC of the plasma sample after oral administration at a single dose of 50 mg/kg BG; (d) TIC of blank plasma.

    Article Snippet: HPLC/Q-TOF/MS Conditions Chromatographic experiments were performed on an Agilent 1200 series HPLC system, equipped with binary pump, autosampler, on-line degasser and automatic thermostatic column oven (CA, USA).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry