Structured Review

Seikagaku chondroitinase b
DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without <t>chondroitinase</t> B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.
Chondroitinase B, supplied by Seikagaku, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chondroitinase b/product/Seikagaku
Average 89 stars, based on 3 article reviews
Price from $9.99 to $1999.99
chondroitinase b - by Bioz Stars, 2020-11
89/100 stars

Images

1) Product Images from "Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1"

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1

Journal: Journal of Clinical Investigation

doi:

DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.
Figure Legend Snippet: DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.

Techniques Used: Enzyme-linked Immunosorbent Assay, Cell Culture, Purification

2) Product Images from "Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1"

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1

Journal: Journal of Clinical Investigation

doi:

DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.
Figure Legend Snippet: DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.

Techniques Used: Enzyme-linked Immunosorbent Assay, Cell Culture, Purification

3) Product Images from "Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1"

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1

Journal: Journal of Clinical Investigation

doi:

DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.
Figure Legend Snippet: DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.

Techniques Used: Enzyme-linked Immunosorbent Assay, Cell Culture, Purification

4) Product Images from "Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis"

Article Title: Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis

Journal: PLoS ONE

doi: 10.1371/journal.pone.0140279

DKO mice are DS-free. Two-day-old pups were metabolically labeled with 35 S sulfate. Skin decorin CS/DS was extracted and purified (A-C). A) CS/DS chains were analyzed on a size-permeation Superose 6 column. The HS degraded products, which were proportionally increased in the DKO mice, derive from the HS-PGs which co-eluted with decorin in the size permeation column used for PGs separation (see “ Material and methods ”). B) Split products obtained after chondroitinase B treatment were size separated on a Superdex Peptide column and C) the iduronic acid content was calculated. The iduronic acid analysis was conducted in duplicate samples. D) Skin PGs were extracted from E19.5 embryos of different genotypes. Decorin was stained before and after chondroitinase ABC and B treatment.
Figure Legend Snippet: DKO mice are DS-free. Two-day-old pups were metabolically labeled with 35 S sulfate. Skin decorin CS/DS was extracted and purified (A-C). A) CS/DS chains were analyzed on a size-permeation Superose 6 column. The HS degraded products, which were proportionally increased in the DKO mice, derive from the HS-PGs which co-eluted with decorin in the size permeation column used for PGs separation (see “ Material and methods ”). B) Split products obtained after chondroitinase B treatment were size separated on a Superdex Peptide column and C) the iduronic acid content was calculated. The iduronic acid analysis was conducted in duplicate samples. D) Skin PGs were extracted from E19.5 embryos of different genotypes. Decorin was stained before and after chondroitinase ABC and B treatment.

Techniques Used: Mouse Assay, Metabolic Labelling, Labeling, Purification, Staining

5) Product Images from "Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis"

Article Title: Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis

Journal: PLoS ONE

doi: 10.1371/journal.pone.0140279

DKO mice are DS-free. Two-day-old pups were metabolically labeled with 35 S sulfate. Skin decorin CS/DS was extracted and purified (A-C). A) CS/DS chains were analyzed on a size-permeation Superose 6 column. The HS degraded products, which were proportionally increased in the DKO mice, derive from the HS-PGs which co-eluted with decorin in the size permeation column used for PGs separation (see “ Material and methods ”). B) Split products obtained after chondroitinase B treatment were size separated on a Superdex Peptide column and C) the iduronic acid content was calculated. The iduronic acid analysis was conducted in duplicate samples. D) Skin PGs were extracted from E19.5 embryos of different genotypes. Decorin was stained before and after chondroitinase ABC and B treatment.
Figure Legend Snippet: DKO mice are DS-free. Two-day-old pups were metabolically labeled with 35 S sulfate. Skin decorin CS/DS was extracted and purified (A-C). A) CS/DS chains were analyzed on a size-permeation Superose 6 column. The HS degraded products, which were proportionally increased in the DKO mice, derive from the HS-PGs which co-eluted with decorin in the size permeation column used for PGs separation (see “ Material and methods ”). B) Split products obtained after chondroitinase B treatment were size separated on a Superdex Peptide column and C) the iduronic acid content was calculated. The iduronic acid analysis was conducted in duplicate samples. D) Skin PGs were extracted from E19.5 embryos of different genotypes. Decorin was stained before and after chondroitinase ABC and B treatment.

Techniques Used: Mouse Assay, Metabolic Labelling, Labeling, Purification, Staining

6) Product Images from "Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis"

Article Title: Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis

Journal: PLoS ONE

doi: 10.1371/journal.pone.0140279

DKO mice are DS-free. Two-day-old pups were metabolically labeled with 35 S sulfate. Skin decorin CS/DS was extracted and purified (A-C). A) CS/DS chains were analyzed on a size-permeation Superose 6 column. The HS degraded products, which were proportionally increased in the DKO mice, derive from the HS-PGs which co-eluted with decorin in the size permeation column used for PGs separation (see “ Material and methods ”). B) Split products obtained after chondroitinase B treatment were size separated on a Superdex Peptide column and C) the iduronic acid content was calculated. The iduronic acid analysis was conducted in duplicate samples. D) Skin PGs were extracted from E19.5 embryos of different genotypes. Decorin was stained before and after chondroitinase ABC and B treatment.
Figure Legend Snippet: DKO mice are DS-free. Two-day-old pups were metabolically labeled with 35 S sulfate. Skin decorin CS/DS was extracted and purified (A-C). A) CS/DS chains were analyzed on a size-permeation Superose 6 column. The HS degraded products, which were proportionally increased in the DKO mice, derive from the HS-PGs which co-eluted with decorin in the size permeation column used for PGs separation (see “ Material and methods ”). B) Split products obtained after chondroitinase B treatment were size separated on a Superdex Peptide column and C) the iduronic acid content was calculated. The iduronic acid analysis was conducted in duplicate samples. D) Skin PGs were extracted from E19.5 embryos of different genotypes. Decorin was stained before and after chondroitinase ABC and B treatment.

Techniques Used: Mouse Assay, Metabolic Labelling, Labeling, Purification, Staining

7) Product Images from "Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1"

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1

Journal: Journal of Clinical Investigation

doi:

DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.
Figure Legend Snippet: DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.

Techniques Used: Enzyme-linked Immunosorbent Assay, Cell Culture, Purification

8) Product Images from "Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1"

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1

Journal: Journal of Clinical Investigation

doi:

DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.
Figure Legend Snippet: DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.

Techniques Used: Enzyme-linked Immunosorbent Assay, Cell Culture, Purification

9) Product Images from "Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1"

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1

Journal: Journal of Clinical Investigation

doi:

DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.
Figure Legend Snippet: DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.

Techniques Used: Enzyme-linked Immunosorbent Assay, Cell Culture, Purification

10) Product Images from "Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis"

Article Title: Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis

Journal: PLoS ONE

doi: 10.1371/journal.pone.0140279

DKO mice are DS-free. Two-day-old pups were metabolically labeled with 35 S sulfate. Skin decorin CS/DS was extracted and purified (A-C). A) CS/DS chains were analyzed on a size-permeation Superose 6 column. The HS degraded products, which were proportionally increased in the DKO mice, derive from the HS-PGs which co-eluted with decorin in the size permeation column used for PGs separation (see “ Material and methods ”). B) Split products obtained after chondroitinase B treatment were size separated on a Superdex Peptide column and C) the iduronic acid content was calculated. The iduronic acid analysis was conducted in duplicate samples. D) Skin PGs were extracted from E19.5 embryos of different genotypes. Decorin was stained before and after chondroitinase ABC and B treatment.
Figure Legend Snippet: DKO mice are DS-free. Two-day-old pups were metabolically labeled with 35 S sulfate. Skin decorin CS/DS was extracted and purified (A-C). A) CS/DS chains were analyzed on a size-permeation Superose 6 column. The HS degraded products, which were proportionally increased in the DKO mice, derive from the HS-PGs which co-eluted with decorin in the size permeation column used for PGs separation (see “ Material and methods ”). B) Split products obtained after chondroitinase B treatment were size separated on a Superdex Peptide column and C) the iduronic acid content was calculated. The iduronic acid analysis was conducted in duplicate samples. D) Skin PGs were extracted from E19.5 embryos of different genotypes. Decorin was stained before and after chondroitinase ABC and B treatment.

Techniques Used: Mouse Assay, Metabolic Labelling, Labeling, Purification, Staining

Related Articles

Binding Assay:

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1
Article Snippet: .. DS was removed from conditioned media by binding to QAE Sephadex beads rather than enzymatic digestion with chondroitinase B, because enzyme treatment only decreased ICAM-1–inductive activity after ethanol precipitation of digested material. .. Conditioned media could not be used after ethanol precipitation and thus was depleted of DS by the described anion exchange procedure.

Cell Stimulation:

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1
Article Snippet: .. Other potential mechanisms of cell stimulation of NF-kB, such as contaminating hyaluronic acid or lipopolysaccharide , are also not likely, as treatment of DS and WFGAG with chondroitinase B and ethanol precipitation completely inhibited ICAM-1 induction, and the purification procedure used to isolate these GAGs would eliminate hyaluronic acid, other anionic compounds, or temperature-sensitive proteins. .. Therefore, although it is unclear whether DS functions through a direct or indirect mechanism, it is clear that it has a potent capacity to activate NF-κB and lead to increased ICAM-1 expression in vitro and in vivo .

Ethanol Precipitation:

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1
Article Snippet: .. DS was removed from conditioned media by binding to QAE Sephadex beads rather than enzymatic digestion with chondroitinase B, because enzyme treatment only decreased ICAM-1–inductive activity after ethanol precipitation of digested material. .. Conditioned media could not be used after ethanol precipitation and thus was depleted of DS by the described anion exchange procedure.

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1
Article Snippet: .. Other potential mechanisms of cell stimulation of NF-kB, such as contaminating hyaluronic acid or lipopolysaccharide , are also not likely, as treatment of DS and WFGAG with chondroitinase B and ethanol precipitation completely inhibited ICAM-1 induction, and the purification procedure used to isolate these GAGs would eliminate hyaluronic acid, other anionic compounds, or temperature-sensitive proteins. .. Therefore, although it is unclear whether DS functions through a direct or indirect mechanism, it is clear that it has a potent capacity to activate NF-κB and lead to increased ICAM-1 expression in vitro and in vivo .

Purification:

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1
Article Snippet: .. Specificity of digestion with chondroitinase B was confirmed both by attempted digestions of DS with heparatinase (Seikagaku America Inc.) or measurement of chondroitinase B activity against purified heparan sulfate. ..

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1
Article Snippet: .. Other potential mechanisms of cell stimulation of NF-kB, such as contaminating hyaluronic acid or lipopolysaccharide , are also not likely, as treatment of DS and WFGAG with chondroitinase B and ethanol precipitation completely inhibited ICAM-1 induction, and the purification procedure used to isolate these GAGs would eliminate hyaluronic acid, other anionic compounds, or temperature-sensitive proteins. .. Therefore, although it is unclear whether DS functions through a direct or indirect mechanism, it is clear that it has a potent capacity to activate NF-κB and lead to increased ICAM-1 expression in vitro and in vivo .

other:

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1
Article Snippet: To determine whether DS in wounds was responsible for ICAM-1 induction, WFGAG or pure DS was treated with chondroitinase B specifically to degrade DS.

Article Title: Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis
Article Snippet: Decorin is degraded to its core protein by chondroitinase B when extracted from Dse +/+ /Dsel +/+ , Dse +/+ /Dsel -/- and Dse +/- /Dsel -/- skin (the latter data not shown for brevity), but is unaffected when extracted from DKO skin ( ).

Activity Assay:

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1
Article Snippet: .. DS was removed from conditioned media by binding to QAE Sephadex beads rather than enzymatic digestion with chondroitinase B, because enzyme treatment only decreased ICAM-1–inductive activity after ethanol precipitation of digested material. .. Conditioned media could not be used after ethanol precipitation and thus was depleted of DS by the described anion exchange procedure.

Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1
Article Snippet: .. Specificity of digestion with chondroitinase B was confirmed both by attempted digestions of DS with heparatinase (Seikagaku America Inc.) or measurement of chondroitinase B activity against purified heparan sulfate. ..

Mouse Assay:

Article Title: Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis
Article Snippet: .. IdoA was 8.4% of total hexuronic acid in chains from the Dse +/+ /Dsel -/- mice and was reduced to 5.6% in Dse +/- Dsel -/- chains, and CS chains from DKO pups were also not cleaved by chondroitinase B. .. Another methodological approach to show the absence of IdoA in DKO mice was to extract PGs from whole skin of E19.5 embryos and to visualize decorin by Western blotting after treatment with chondroitinase ABC, which completely degrades the CS/DS chain to disaccharides irrespective of the epimer configuration, compared to digestion with chondroitinase B only.

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    Seikagaku chondroitinase b
    DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without <t>chondroitinase</t> B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.
    Chondroitinase B, supplied by Seikagaku, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chondroitinase b/product/Seikagaku
    Average 89 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    chondroitinase b - by Bioz Stars, 2020-11
    89/100 stars
      Buy from Supplier

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    DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.

    Journal: Journal of Clinical Investigation

    Article Title: Dermatan sulfate activates nuclear factor-?b and induces endothelial and circulating intercellular adhesion molecule-1

    doi:

    Figure Lengend Snippet: DS in wound fluid induces cell surface ICAM-1. Cell surface ICAM-1 was measured on human dermal microvascular endothelial cells by ELISA, as described in Methods. ( a ) Cells cultured for 24 hours with various concentrations of total soluble GAG purified from human wound fluids. ( b ) Control media, WFGAG (4 μg/mL), or pure DS (20 μg/mL) was treated with or without chondroitinase B (CHase B), precipitated, and then added to endothelial cells cultured for 24 hours in defined media containing 1% FCS. Data represents mean relative cell surface ICAM-1 content expressed as mean OD/min ± SD for triplicate determinations.

    Article Snippet: For experiments in which DS was enzymatically digested before addition to endothelial cells and measurement of cell surface ICAM-1, WFGAG or commercial DS was treated for 4 hours at 37°C with 2 U chondroitinase B (Seikagaku America Inc.) in 50 mM Tris, 50 mM NaCl, 4 mM CaCl2 (pH 8.0).

    Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Purification

    DKO mice are DS-free. Two-day-old pups were metabolically labeled with 35 S sulfate. Skin decorin CS/DS was extracted and purified (A-C). A) CS/DS chains were analyzed on a size-permeation Superose 6 column. The HS degraded products, which were proportionally increased in the DKO mice, derive from the HS-PGs which co-eluted with decorin in the size permeation column used for PGs separation (see “ Material and methods ”). B) Split products obtained after chondroitinase B treatment were size separated on a Superdex Peptide column and C) the iduronic acid content was calculated. The iduronic acid analysis was conducted in duplicate samples. D) Skin PGs were extracted from E19.5 embryos of different genotypes. Decorin was stained before and after chondroitinase ABC and B treatment.

    Journal: PLoS ONE

    Article Title: Dermatan Sulfate-Free Mice Display Embryological Defects and Are Neonatal Lethal Despite Normal Lymphoid and Non-Lymphoid Organogenesis

    doi: 10.1371/journal.pone.0140279

    Figure Lengend Snippet: DKO mice are DS-free. Two-day-old pups were metabolically labeled with 35 S sulfate. Skin decorin CS/DS was extracted and purified (A-C). A) CS/DS chains were analyzed on a size-permeation Superose 6 column. The HS degraded products, which were proportionally increased in the DKO mice, derive from the HS-PGs which co-eluted with decorin in the size permeation column used for PGs separation (see “ Material and methods ”). B) Split products obtained after chondroitinase B treatment were size separated on a Superdex Peptide column and C) the iduronic acid content was calculated. The iduronic acid analysis was conducted in duplicate samples. D) Skin PGs were extracted from E19.5 embryos of different genotypes. Decorin was stained before and after chondroitinase ABC and B treatment.

    Article Snippet: Quantification and spatial arrangement of IdoA along the chain was analyzed by cleaving 40,000 dpm of CS/DS with 2 mIU of chondroitinase B (Seikagaku) in 20 mM Hepes, pH 7.2, 50 mM NaCl, 4 mM CaCl2 , and 0.1 mg/ml BSA for 90 min.

    Techniques: Mouse Assay, Metabolic Labelling, Labeling, Purification, Staining