Chondroitinase B, supplied by IBEX Technologies, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chondroitinase b/product/IBEX Technologies
Average 89 stars, based on 1 article reviews
Price from $9.99 to $1999.99
1) Product Images from "Heparan sulfate proteoglycans mediate Aβ-induced oxidative stress and hypercontractility in cultured vascular smooth muscle cells"
Article Title: Heparan sulfate proteoglycans mediate Aβ-induced oxidative stress and hypercontractility in cultured vascular smooth muscle cells
Journal: Molecular Neurodegeneration
Figure Legend Snippet: Pharmacological knockdown of other glycosaminoglycan (GAG) family members does not affect Aβ 1-40 -mediated ROS production in VSMC. Primary human cerebral VSMC were pre-treated with chondroitinase B (10 -1 IU/mL; selectively degrades dermatin sulfate; panel a ), chondroitinase AC (10 -1 IU/mL; selectively degrades chondroitin sulfate; panel a ), or varying concentrations of the sulfation inhibitor sodium chlorate (5-50 mM; panel b ) for 2 h, washed, loaded with Mitotracker Red CM-H 2 XRos (MTR; 5 μM), and treated with Aβ 1-40 . In some cases, cells were pre-treated with heat-inactivated (HI) enzyme (at the same concentration of active enzyme) and washed prior to MTR loading and Aβ treatment. Fluorescence was measured after 30 minutes. Results are representative of 3 independent experiments performed in triplicate. *p
Techniques Used: Concentration Assay, Fluorescence
2) Product Images from "Primary Ovarian Carcinomas and Abdominal Metastasis Contain 4,6-Disulfated Chondroitin Sulfate Rich Regions, Which Provide Adhesive Properties to Tumour Cells"
Article Title: Primary Ovarian Carcinomas and Abdominal Metastasis Contain 4,6-Disulfated Chondroitin Sulfate Rich Regions, Which Provide Adhesive Properties to Tumour Cells
Journal: PLoS ONE
Figure Legend Snippet: Spheroid formation by SKOV3 cells depends on chondroitin sulfate. A) Spheroid formation (tumour cell aggregation) was inhibited by enzymatic digestion using either of chondroitinase-ABC or –AC but not by chondroitinase B. Bar: 200 µm B) Spheroids formed by wild type SKOV3 cells and cells transfected with GalNAc4S-6ST and empty vector. Note the smooth and roundish appearance of GalNAc4S-6ST transfected cells. Bar: 100 µm.
Techniques Used: Transfection, Plasmid Preparation
Article Title: Reduction in Brain Heparan Sulfate with Systemic Administration of an IgG Trojan Horse-Sulfamidase Fusion Protein in the Mucopolysaccharidosis Type IIIA Mouse.
Article Snippet: .. Mucopolysaccharidosis Type IIIA (MPSIIIA), also known as Sanfilippo A syndrome, is an inherited neurodegenerative
Article Title: Mutations in B3GALT6, which Encodes a Glycosaminoglycan Linker Region Enzyme, Cause a Spectrum of Skeletal and Connective Tissue Disorders
Article Snippet: .. In brief, purified GAG fractions were treated individually with a mixture of chondroitinases ABC and AC-II, a mixture of chondroitinases AC-I (EC 18.104.22.168) (Seikagaku Corp.) and AC-II (EC 22.214.171.124) (Seikagaku Corp.),