Structured Review

IBEX Technologies chondroitinase b
Pharmacological knockdown of other glycosaminoglycan (GAG) family members does not affect Aβ 1-40 -mediated ROS production in VSMC. Primary human cerebral VSMC were pre-treated with <t>chondroitinase</t> B (10 -1 IU/mL; selectively degrades dermatin sulfate; panel a ), chondroitinase AC (10 -1 IU/mL; selectively degrades chondroitin sulfate; panel a ), or varying concentrations of the sulfation inhibitor sodium chlorate (5-50 mM; panel b ) for 2 h, washed, loaded with Mitotracker Red CM-H 2 XRos (MTR; 5 μM), and treated with Aβ 1-40 . In some cases, cells were pre-treated with heat-inactivated (HI) enzyme (at the same concentration of active enzyme) and washed prior to MTR loading and Aβ treatment. Fluorescence was measured after 30 minutes. Results are representative of 3 independent experiments performed in triplicate. *p
Chondroitinase B, supplied by IBEX Technologies, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chondroitinase b/product/IBEX Technologies
Average 89 stars, based on 1 article reviews
Price from $9.99 to $1999.99
chondroitinase b - by Bioz Stars, 2020-11
89/100 stars

Images

1) Product Images from "Heparan sulfate proteoglycans mediate Aβ-induced oxidative stress and hypercontractility in cultured vascular smooth muscle cells"

Article Title: Heparan sulfate proteoglycans mediate Aβ-induced oxidative stress and hypercontractility in cultured vascular smooth muscle cells

Journal: Molecular Neurodegeneration

doi: 10.1186/s13024-016-0073-8

Pharmacological knockdown of other glycosaminoglycan (GAG) family members does not affect Aβ 1-40 -mediated ROS production in VSMC. Primary human cerebral VSMC were pre-treated with chondroitinase B (10 -1 IU/mL; selectively degrades dermatin sulfate; panel a ), chondroitinase AC (10 -1 IU/mL; selectively degrades chondroitin sulfate; panel a ), or varying concentrations of the sulfation inhibitor sodium chlorate (5-50 mM; panel b ) for 2 h, washed, loaded with Mitotracker Red CM-H 2 XRos (MTR; 5 μM), and treated with Aβ 1-40 . In some cases, cells were pre-treated with heat-inactivated (HI) enzyme (at the same concentration of active enzyme) and washed prior to MTR loading and Aβ treatment. Fluorescence was measured after 30 minutes. Results are representative of 3 independent experiments performed in triplicate. *p
Figure Legend Snippet: Pharmacological knockdown of other glycosaminoglycan (GAG) family members does not affect Aβ 1-40 -mediated ROS production in VSMC. Primary human cerebral VSMC were pre-treated with chondroitinase B (10 -1 IU/mL; selectively degrades dermatin sulfate; panel a ), chondroitinase AC (10 -1 IU/mL; selectively degrades chondroitin sulfate; panel a ), or varying concentrations of the sulfation inhibitor sodium chlorate (5-50 mM; panel b ) for 2 h, washed, loaded with Mitotracker Red CM-H 2 XRos (MTR; 5 μM), and treated with Aβ 1-40 . In some cases, cells were pre-treated with heat-inactivated (HI) enzyme (at the same concentration of active enzyme) and washed prior to MTR loading and Aβ treatment. Fluorescence was measured after 30 minutes. Results are representative of 3 independent experiments performed in triplicate. *p

Techniques Used: Concentration Assay, Fluorescence

2) Product Images from "Primary Ovarian Carcinomas and Abdominal Metastasis Contain 4,6-Disulfated Chondroitin Sulfate Rich Regions, Which Provide Adhesive Properties to Tumour Cells"

Article Title: Primary Ovarian Carcinomas and Abdominal Metastasis Contain 4,6-Disulfated Chondroitin Sulfate Rich Regions, Which Provide Adhesive Properties to Tumour Cells

Journal: PLoS ONE

doi: 10.1371/journal.pone.0111806

Spheroid formation by SKOV3 cells depends on chondroitin sulfate. A) Spheroid formation (tumour cell aggregation) was inhibited by enzymatic digestion using either of chondroitinase-ABC or –AC but not by chondroitinase B. Bar: 200 µm B) Spheroids formed by wild type SKOV3 cells and cells transfected with GalNAc4S-6ST and empty vector. Note the smooth and roundish appearance of GalNAc4S-6ST transfected cells. Bar: 100 µm.
Figure Legend Snippet: Spheroid formation by SKOV3 cells depends on chondroitin sulfate. A) Spheroid formation (tumour cell aggregation) was inhibited by enzymatic digestion using either of chondroitinase-ABC or –AC but not by chondroitinase B. Bar: 200 µm B) Spheroids formed by wild type SKOV3 cells and cells transfected with GalNAc4S-6ST and empty vector. Note the smooth and roundish appearance of GalNAc4S-6ST transfected cells. Bar: 100 µm.

Techniques Used: Transfection, Plasmid Preparation

Related Articles

Recombinant:

Article Title: Reduction in Brain Heparan Sulfate with Systemic Administration of an IgG Trojan Horse-Sulfamidase Fusion Protein in the Mucopolysaccharidosis Type IIIA Mouse.
Article Snippet: .. Mucopolysaccharidosis Type IIIA (MPSIIIA), also known as Sanfilippo A syndrome, is an inherited neurodegenerative disease caused by mutations in the lysosomal enzyme, N-sulfoglucosamine sulfohydrolase (SGSH), also known as sulfamidase. ..

Purification:

Article Title: Mutations in B3GALT6, which Encodes a Glycosaminoglycan Linker Region Enzyme, Cause a Spectrum of Skeletal and Connective Tissue Disorders
Article Snippet: .. In brief, purified GAG fractions were treated individually with a mixture of chondroitinases ABC and AC-II, a mixture of chondroitinases AC-I (EC 4.2.2.5) (Seikagaku Corp.) and AC-II (EC 4.2.2.5) (Seikagaku Corp.), chondroitinase B (EC 4.2.2.19) (IBEX Technologies), or a mixture of heparinases-I and -III (IBEX Technologies) for analyzing the disaccharide composition of CS/DS, CS, DS, and HS, respectively. .. The digests were labeled with a fluorophore 2-aminobenzamide (2AB) and aliquots of the 2AB derivatives of CS/DS/HS disaccharides were analyzed by anion-exchange HPLC on a PA-03 column (YMC Co.).

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    IBEX Technologies chondroitinase b
    Pharmacological knockdown of other glycosaminoglycan (GAG) family members does not affect Aβ 1-40 -mediated ROS production in VSMC. Primary human cerebral VSMC were pre-treated with <t>chondroitinase</t> B (10 -1 IU/mL; selectively degrades dermatin sulfate; panel a ), chondroitinase AC (10 -1 IU/mL; selectively degrades chondroitin sulfate; panel a ), or varying concentrations of the sulfation inhibitor sodium chlorate (5-50 mM; panel b ) for 2 h, washed, loaded with Mitotracker Red CM-H 2 XRos (MTR; 5 μM), and treated with Aβ 1-40 . In some cases, cells were pre-treated with heat-inactivated (HI) enzyme (at the same concentration of active enzyme) and washed prior to MTR loading and Aβ treatment. Fluorescence was measured after 30 minutes. Results are representative of 3 independent experiments performed in triplicate. *p
    Chondroitinase B, supplied by IBEX Technologies, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chondroitinase b/product/IBEX Technologies
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chondroitinase b - by Bioz Stars, 2020-11
    89/100 stars
      Buy from Supplier

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    Pharmacological knockdown of other glycosaminoglycan (GAG) family members does not affect Aβ 1-40 -mediated ROS production in VSMC. Primary human cerebral VSMC were pre-treated with chondroitinase B (10 -1 IU/mL; selectively degrades dermatin sulfate; panel a ), chondroitinase AC (10 -1 IU/mL; selectively degrades chondroitin sulfate; panel a ), or varying concentrations of the sulfation inhibitor sodium chlorate (5-50 mM; panel b ) for 2 h, washed, loaded with Mitotracker Red CM-H 2 XRos (MTR; 5 μM), and treated with Aβ 1-40 . In some cases, cells were pre-treated with heat-inactivated (HI) enzyme (at the same concentration of active enzyme) and washed prior to MTR loading and Aβ treatment. Fluorescence was measured after 30 minutes. Results are representative of 3 independent experiments performed in triplicate. *p

    Journal: Molecular Neurodegeneration

    Article Title: Heparan sulfate proteoglycans mediate Aβ-induced oxidative stress and hypercontractility in cultured vascular smooth muscle cells

    doi: 10.1186/s13024-016-0073-8

    Figure Lengend Snippet: Pharmacological knockdown of other glycosaminoglycan (GAG) family members does not affect Aβ 1-40 -mediated ROS production in VSMC. Primary human cerebral VSMC were pre-treated with chondroitinase B (10 -1 IU/mL; selectively degrades dermatin sulfate; panel a ), chondroitinase AC (10 -1 IU/mL; selectively degrades chondroitin sulfate; panel a ), or varying concentrations of the sulfation inhibitor sodium chlorate (5-50 mM; panel b ) for 2 h, washed, loaded with Mitotracker Red CM-H 2 XRos (MTR; 5 μM), and treated with Aβ 1-40 . In some cases, cells were pre-treated with heat-inactivated (HI) enzyme (at the same concentration of active enzyme) and washed prior to MTR loading and Aβ treatment. Fluorescence was measured after 30 minutes. Results are representative of 3 independent experiments performed in triplicate. *p

    Article Snippet: Chondroitinase B and AC were purchased from IBEX Technologies Inc. (Montreal, Quebec, Canada).

    Techniques: Concentration Assay, Fluorescence

    Spheroid formation by SKOV3 cells depends on chondroitin sulfate. A) Spheroid formation (tumour cell aggregation) was inhibited by enzymatic digestion using either of chondroitinase-ABC or –AC but not by chondroitinase B. Bar: 200 µm B) Spheroids formed by wild type SKOV3 cells and cells transfected with GalNAc4S-6ST and empty vector. Note the smooth and roundish appearance of GalNAc4S-6ST transfected cells. Bar: 100 µm.

    Journal: PLoS ONE

    Article Title: Primary Ovarian Carcinomas and Abdominal Metastasis Contain 4,6-Disulfated Chondroitin Sulfate Rich Regions, Which Provide Adhesive Properties to Tumour Cells

    doi: 10.1371/journal.pone.0111806

    Figure Lengend Snippet: Spheroid formation by SKOV3 cells depends on chondroitin sulfate. A) Spheroid formation (tumour cell aggregation) was inhibited by enzymatic digestion using either of chondroitinase-ABC or –AC but not by chondroitinase B. Bar: 200 µm B) Spheroids formed by wild type SKOV3 cells and cells transfected with GalNAc4S-6ST and empty vector. Note the smooth and roundish appearance of GalNAc4S-6ST transfected cells. Bar: 100 µm.

    Article Snippet: To further study the adhesive properties of CS wildtype SKOV3 cells in hanging drops were digested with specific glycosaminoglycan degrading enzymes including chondroitinase ABC (Sigma Aldrich, St. Louis, USA), chondroitinase B and chondroitinase AC (IBEX Technologies, Montreal, Canada).

    Techniques: Transfection, Plasmid Preparation