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steraloids inc cholestenone
Transition temperatures ( top ) and vesicle micrographs ( bottom ) for vesicles of 1:1:(1 − x ): x <t>DOPC/DPPC/cholesterol/cholestenone</t> with 0.8 mol % TR-DPPE. Coexisting liquid phases are observed below the miscibility transition temperature in membranes with more cholesterol (▴), and solid and liquid phases are observed below the melting transition in membranes with more cholestenone (▪). In membranes with an equal ratio of cholesterol and cholestenone (•), fluctuations at domains boundaries relax slowly (∼1–10 s) and dark domains may contain a solid phase. Vesicles contain x = 0%, 25%, 50%, 75%, and 100% cholestenone (from left to right ) and are imaged at 16°C. All scale bars are 20 μ m.
Cholestenone, supplied by steraloids inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Sterol Structure Determines Miscibility versus Melting Transitions in Lipid Vesicles"

Article Title: Sterol Structure Determines Miscibility versus Melting Transitions in Lipid Vesicles

Journal: Biophysical Journal

doi: 10.1529/biophysj.104.049635

Transition temperatures ( top ) and vesicle micrographs ( bottom ) for vesicles of 1:1:(1 − x ): x DOPC/DPPC/cholesterol/cholestenone with 0.8 mol % TR-DPPE. Coexisting liquid phases are observed below the miscibility transition temperature in membranes with more cholesterol (▴), and solid and liquid phases are observed below the melting transition in membranes with more cholestenone (▪). In membranes with an equal ratio of cholesterol and cholestenone (•), fluctuations at domains boundaries relax slowly (∼1–10 s) and dark domains may contain a solid phase. Vesicles contain x = 0%, 25%, 50%, 75%, and 100% cholestenone (from left to right ) and are imaged at 16°C. All scale bars are 20 μ m.
Figure Legend Snippet: Transition temperatures ( top ) and vesicle micrographs ( bottom ) for vesicles of 1:1:(1 − x ): x DOPC/DPPC/cholesterol/cholestenone with 0.8 mol % TR-DPPE. Coexisting liquid phases are observed below the miscibility transition temperature in membranes with more cholesterol (▴), and solid and liquid phases are observed below the melting transition in membranes with more cholestenone (▪). In membranes with an equal ratio of cholesterol and cholestenone (•), fluctuations at domains boundaries relax slowly (∼1–10 s) and dark domains may contain a solid phase. Vesicles contain x = 0%, 25%, 50%, 75%, and 100% cholestenone (from left to right ) and are imaged at 16°C. All scale bars are 20 μ m.

Techniques Used:

Fluorescence micrographs of vesicles produced from 1:1:1 DOPC/DPPC/sterol with 0.8 mol % TR-DPPE at 23°C. Vesicles contain ( a ) 25-hydroxycholesterol, ( b ) cholesterol, ( c ) dihydrocholesterol, ( d ) epicholesterol, ( e ) ergosterol, ( f ) no sterol (control), ( g ) androstenolone, ( h ) cholestane, ( i ) cholestenone, and ( j ) coprostanol. As temperature is lowered, phase coexistence is observed between either ( top row ) two liquid phases or ( bottom row ) a solid and liquid phase. With time, liquid phases coalesce to form one bright domain and one dark domain. Scale bars are 10 μ m.
Figure Legend Snippet: Fluorescence micrographs of vesicles produced from 1:1:1 DOPC/DPPC/sterol with 0.8 mol % TR-DPPE at 23°C. Vesicles contain ( a ) 25-hydroxycholesterol, ( b ) cholesterol, ( c ) dihydrocholesterol, ( d ) epicholesterol, ( e ) ergosterol, ( f ) no sterol (control), ( g ) androstenolone, ( h ) cholestane, ( i ) cholestenone, and ( j ) coprostanol. As temperature is lowered, phase coexistence is observed between either ( top row ) two liquid phases or ( bottom row ) a solid and liquid phase. With time, liquid phases coalesce to form one bright domain and one dark domain. Scale bars are 10 μ m.

Techniques Used: Fluorescence, Produced

2) Product Images from "Phase Behavior of Lipid Monolayers Containing DPPC and Cholesterol Analogs"

Article Title: Phase Behavior of Lipid Monolayers Containing DPPC and Cholesterol Analogs

Journal:

doi: 10.1529/biophysj.105.072959

Phase diagrams for monolayers of DPPC-containing membrane-inactive sterols. Monolayers containing androstenolone, lanosterol, cholestenone, and coprostanol exhibit no liquid-liquid immiscibility for all concentrations studied (denoted by “x”
Figure Legend Snippet: Phase diagrams for monolayers of DPPC-containing membrane-inactive sterols. Monolayers containing androstenolone, lanosterol, cholestenone, and coprostanol exhibit no liquid-liquid immiscibility for all concentrations studied (denoted by “x”

Techniques Used:

3) Product Images from "Sterol Structure Strongly Modulates Membrane-IAPP Interactions"

Article Title: Sterol Structure Strongly Modulates Membrane-IAPP Interactions

Journal: Biochemistry

doi: 10.1021/acs.biochem.7b01190

Effect of different sterols upon amyloid formation. Vesicles were prepared with different sterols and the zwitterionic lipid POPC. The results of thioflavin-T assays are displayed. Data are plotted for hIAPP in solution (black), LUVs containing 100 mol% POPC (red) or LUVs containing 80 mol% POPC and 20 mol% of the following sterols: cholesterol (blue), cholestanol (cyan), lathosterol (dark cyan), 7-dehydrocholesterol (yellow), epicholesterol (green), pregnenolone (pink), cholestenone (dark red), coprostanol (purple) and 5α-cholestan-3 one (grey). Experiments were conducted in 20 mM Tris·HCl, 100 mM NaCl, pH 7.4 at 25 °C with 400 μM lipid and 20 μM hIAPP.
Figure Legend Snippet: Effect of different sterols upon amyloid formation. Vesicles were prepared with different sterols and the zwitterionic lipid POPC. The results of thioflavin-T assays are displayed. Data are plotted for hIAPP in solution (black), LUVs containing 100 mol% POPC (red) or LUVs containing 80 mol% POPC and 20 mol% of the following sterols: cholesterol (blue), cholestanol (cyan), lathosterol (dark cyan), 7-dehydrocholesterol (yellow), epicholesterol (green), pregnenolone (pink), cholestenone (dark red), coprostanol (purple) and 5α-cholestan-3 one (grey). Experiments were conducted in 20 mM Tris·HCl, 100 mM NaCl, pH 7.4 at 25 °C with 400 μM lipid and 20 μM hIAPP.

Techniques Used:

Effect of different sterols on membrane leakage. The time course of carboxyfluorescein leakage experiments are displayed for vesicles containing 100 mol% POPC (red), 80 mol% POPC and 20 mol% sterol: cholesterol (blue), cholestanol (cyan), lathosterol (dark cyan), 7-dehydrocholesterol (yellow), epicholesterol (green), pregnenolone (pink), cholestenone (dark red), coprostanol (purple) and 5α-cholestan-3 one (grey). Experiments were conducted in 20 mM Tris·HCl, 100 mM NaCl, pH 7.4 at 25 °C with 400 μM lipid and 20 μM hIAPP.
Figure Legend Snippet: Effect of different sterols on membrane leakage. The time course of carboxyfluorescein leakage experiments are displayed for vesicles containing 100 mol% POPC (red), 80 mol% POPC and 20 mol% sterol: cholesterol (blue), cholestanol (cyan), lathosterol (dark cyan), 7-dehydrocholesterol (yellow), epicholesterol (green), pregnenolone (pink), cholestenone (dark red), coprostanol (purple) and 5α-cholestan-3 one (grey). Experiments were conducted in 20 mM Tris·HCl, 100 mM NaCl, pH 7.4 at 25 °C with 400 μM lipid and 20 μM hIAPP.

Techniques Used:

Related Articles

Chromatography:

Article Title: Phase Behavior of Lipid Monolayers Containing DPPC and Cholesterol Analogs
Article Snippet: Androstenolone (5-androsten-3 β -ol-17-one), cholestane (5 α -cholestane), cholestenone (4-cholesten-3-one), coprostanol (5 β -cholestan-3 β -ol), and epicholesterol (5-cholesten-3 α -ol) were obtained from Steraloids (Newport, RI). .. Lipid stock solutions were mixed at 1–10 mg/ml in high-performance liquid chromatography-grade chloroform and stored at −20°C until use.

Sublimation:

Article Title: Sterol Structure Determines Miscibility versus Melting Transitions in Lipid Vesicles
Article Snippet: Androstenolone (5-androsten-3 β -ol-17-one), coprostanol (5 β -cholestan-3 β -ol), cholestenone (4-cholesten-3-one), cholestane (5 α -cholestane), and epicholesterol (5-cholesten-3 α -ol) were obtained from Steraloids (Newport, RI). .. Vesicles were produced with 33 mol % sterol because vesicles of 1:1:1 DOPC/DPPC/cholesterol are comfortably far from the gel phase transition that appears at low sterol concentrations ( ) and from the solubility limit of cholesterol in vesicles at high sterol concentrations ( ).

Solubility:

Article Title: Sterol Structure Determines Miscibility versus Melting Transitions in Lipid Vesicles
Article Snippet: Androstenolone (5-androsten-3 β -ol-17-one), coprostanol (5 β -cholestan-3 β -ol), cholestenone (4-cholesten-3-one), cholestane (5 α -cholestane), and epicholesterol (5-cholesten-3 α -ol) were obtained from Steraloids (Newport, RI). .. Vesicles were produced with 33 mol % sterol because vesicles of 1:1:1 DOPC/DPPC/cholesterol are comfortably far from the gel phase transition that appears at low sterol concentrations ( ) and from the solubility limit of cholesterol in vesicles at high sterol concentrations ( ).

Purification:

Article Title: Phase Behavior of Lipid Monolayers Containing DPPC and Cholesterol Analogs
Article Snippet: Androstenolone (5-androsten-3 β -ol-17-one), cholestane (5 α -cholestane), cholestenone (4-cholesten-3-one), coprostanol (5 β -cholestan-3 β -ol), and epicholesterol (5-cholesten-3 α -ol) were obtained from Steraloids (Newport, RI). .. Monolayers were deposited on a subphase of purified water of resistivity > 18 MΩ cm (Barnstead, Dubuque, IA).

Produced:

Article Title: Sterol Structure Determines Miscibility versus Melting Transitions in Lipid Vesicles
Article Snippet: Androstenolone (5-androsten-3 β -ol-17-one), coprostanol (5 β -cholestan-3 β -ol), cholestenone (4-cholesten-3-one), cholestane (5 α -cholestane), and epicholesterol (5-cholesten-3 α -ol) were obtained from Steraloids (Newport, RI). .. Vesicles were produced with 33 mol % sterol because vesicles of 1:1:1 DOPC/DPPC/cholesterol are comfortably far from the gel phase transition that appears at low sterol concentrations ( ) and from the solubility limit of cholesterol in vesicles at high sterol concentrations ( ).

Thin Layer Chromatography:

Article Title: Sterol Structure Strongly Modulates Membrane-IAPP Interactions
Article Snippet: 5α-cholestan-3-one, cholestenone, coprostanol, and epicholesterol were obtained from Steraloids. .. Sterol purity were assayed by TLC and no significant impurities were detected.

Article Title: Effects of host cell sterol composition upon internalization of Yersinia pseudotuberculosis and clustered β1 integrin
Article Snippet: Coprostanol, desmosterol, epicholesterol (5-cholesten-3α-ol), allocholesterol (4-cholesten-3β-ol), cholesta-4,6-dien-3β-ol, androstenol (5-androsten-3β-ol), and cholestenone (4-cholesten-3-one) were obtained from Steraloids Inc. (Newport, RI). .. The sterols generally appeared as single bands on thin layer chromatography, but lanosterol was purchased as a mixture with ∼65% lanosterol, the remainder likely being dihydrolanosterol.

Bradford Protein Assay:

Article Title: Effects of host cell sterol composition upon internalization of Yersinia pseudotuberculosis and clustered β1 integrin
Article Snippet: Coprostanol, desmosterol, epicholesterol (5-cholesten-3α-ol), allocholesterol (4-cholesten-3β-ol), cholesta-4,6-dien-3β-ol, androstenol (5-androsten-3β-ol), and cholestenone (4-cholesten-3-one) were obtained from Steraloids Inc. (Newport, RI). .. Bradford protein assay reagent and 10× PBS (10 m m sodium phosphate, 150 m m NaCl, pH 7.4) used for immunofluorescence were bought from Bio-Rad.

IA:

Article Title: Phase Behavior of Lipid Monolayers Containing DPPC and Cholesterol Analogs
Article Snippet: Androstenolone (5-androsten-3 β -ol-17-one), cholestane (5 α -cholestane), cholestenone (4-cholesten-3-one), coprostanol (5 β -cholestan-3 β -ol), and epicholesterol (5-cholesten-3 α -ol) were obtained from Steraloids (Newport, RI). .. Monolayers were deposited on a subphase of purified water of resistivity > 18 MΩ cm (Barnstead, Dubuque, IA).

Immunofluorescence:

Article Title: Effects of host cell sterol composition upon internalization of Yersinia pseudotuberculosis and clustered β1 integrin
Article Snippet: Coprostanol, desmosterol, epicholesterol (5-cholesten-3α-ol), allocholesterol (4-cholesten-3β-ol), cholesta-4,6-dien-3β-ol, androstenol (5-androsten-3β-ol), and cholestenone (4-cholesten-3-one) were obtained from Steraloids Inc. (Newport, RI). .. Bradford protein assay reagent and 10× PBS (10 m m sodium phosphate, 150 m m NaCl, pH 7.4) used for immunofluorescence were bought from Bio-Rad.

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    steraloids inc cholestenone
    Transition temperatures ( top ) and vesicle micrographs ( bottom ) for vesicles of 1:1:(1 − x ): x <t>DOPC/DPPC/cholesterol/cholestenone</t> with 0.8 mol % TR-DPPE. Coexisting liquid phases are observed below the miscibility transition temperature in membranes with more cholesterol (▴), and solid and liquid phases are observed below the melting transition in membranes with more cholestenone (▪). In membranes with an equal ratio of cholesterol and cholestenone (•), fluctuations at domains boundaries relax slowly (∼1–10 s) and dark domains may contain a solid phase. Vesicles contain x = 0%, 25%, 50%, 75%, and 100% cholestenone (from left to right ) and are imaged at 16°C. All scale bars are 20 μ m.
    Cholestenone, supplied by steraloids inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cholestenone/product/steraloids inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cholestenone - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    86
    steraloids inc cholestenol
    Growth curves for CG388 (A) and CG156 (B) cultured using sterol-free and sterol-supplemented glc YM. 7,22, ergosta 7,22-dienol; ERG, ergosterol; LAN, lanosterol; CAN, cholestanol; CER, cholesterol; CEN, Δ 7 <t>-cholestenol;</t> DES, desmosterol.
    Cholestenol, supplied by steraloids inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cholestenol/product/steraloids inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cholestenol - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Transition temperatures ( top ) and vesicle micrographs ( bottom ) for vesicles of 1:1:(1 − x ): x DOPC/DPPC/cholesterol/cholestenone with 0.8 mol % TR-DPPE. Coexisting liquid phases are observed below the miscibility transition temperature in membranes with more cholesterol (▴), and solid and liquid phases are observed below the melting transition in membranes with more cholestenone (▪). In membranes with an equal ratio of cholesterol and cholestenone (•), fluctuations at domains boundaries relax slowly (∼1–10 s) and dark domains may contain a solid phase. Vesicles contain x = 0%, 25%, 50%, 75%, and 100% cholestenone (from left to right ) and are imaged at 16°C. All scale bars are 20 μ m.

    Journal: Biophysical Journal

    Article Title: Sterol Structure Determines Miscibility versus Melting Transitions in Lipid Vesicles

    doi: 10.1529/biophysj.104.049635

    Figure Lengend Snippet: Transition temperatures ( top ) and vesicle micrographs ( bottom ) for vesicles of 1:1:(1 − x ): x DOPC/DPPC/cholesterol/cholestenone with 0.8 mol % TR-DPPE. Coexisting liquid phases are observed below the miscibility transition temperature in membranes with more cholesterol (▴), and solid and liquid phases are observed below the melting transition in membranes with more cholestenone (▪). In membranes with an equal ratio of cholesterol and cholestenone (•), fluctuations at domains boundaries relax slowly (∼1–10 s) and dark domains may contain a solid phase. Vesicles contain x = 0%, 25%, 50%, 75%, and 100% cholestenone (from left to right ) and are imaged at 16°C. All scale bars are 20 μ m.

    Article Snippet: Androstenolone (5-androsten-3 β -ol-17-one), coprostanol (5 β -cholestan-3 β -ol), cholestenone (4-cholesten-3-one), cholestane (5 α -cholestane), and epicholesterol (5-cholesten-3 α -ol) were obtained from Steraloids (Newport, RI).

    Techniques:

    Fluorescence micrographs of vesicles produced from 1:1:1 DOPC/DPPC/sterol with 0.8 mol % TR-DPPE at 23°C. Vesicles contain ( a ) 25-hydroxycholesterol, ( b ) cholesterol, ( c ) dihydrocholesterol, ( d ) epicholesterol, ( e ) ergosterol, ( f ) no sterol (control), ( g ) androstenolone, ( h ) cholestane, ( i ) cholestenone, and ( j ) coprostanol. As temperature is lowered, phase coexistence is observed between either ( top row ) two liquid phases or ( bottom row ) a solid and liquid phase. With time, liquid phases coalesce to form one bright domain and one dark domain. Scale bars are 10 μ m.

    Journal: Biophysical Journal

    Article Title: Sterol Structure Determines Miscibility versus Melting Transitions in Lipid Vesicles

    doi: 10.1529/biophysj.104.049635

    Figure Lengend Snippet: Fluorescence micrographs of vesicles produced from 1:1:1 DOPC/DPPC/sterol with 0.8 mol % TR-DPPE at 23°C. Vesicles contain ( a ) 25-hydroxycholesterol, ( b ) cholesterol, ( c ) dihydrocholesterol, ( d ) epicholesterol, ( e ) ergosterol, ( f ) no sterol (control), ( g ) androstenolone, ( h ) cholestane, ( i ) cholestenone, and ( j ) coprostanol. As temperature is lowered, phase coexistence is observed between either ( top row ) two liquid phases or ( bottom row ) a solid and liquid phase. With time, liquid phases coalesce to form one bright domain and one dark domain. Scale bars are 10 μ m.

    Article Snippet: Androstenolone (5-androsten-3 β -ol-17-one), coprostanol (5 β -cholestan-3 β -ol), cholestenone (4-cholesten-3-one), cholestane (5 α -cholestane), and epicholesterol (5-cholesten-3 α -ol) were obtained from Steraloids (Newport, RI).

    Techniques: Fluorescence, Produced

    Phase diagrams for monolayers of DPPC-containing membrane-inactive sterols. Monolayers containing androstenolone, lanosterol, cholestenone, and coprostanol exhibit no liquid-liquid immiscibility for all concentrations studied (denoted by “x”

    Journal:

    Article Title: Phase Behavior of Lipid Monolayers Containing DPPC and Cholesterol Analogs

    doi: 10.1529/biophysj.105.072959

    Figure Lengend Snippet: Phase diagrams for monolayers of DPPC-containing membrane-inactive sterols. Monolayers containing androstenolone, lanosterol, cholestenone, and coprostanol exhibit no liquid-liquid immiscibility for all concentrations studied (denoted by “x”

    Article Snippet: Androstenolone (5-androsten-3 β -ol-17-one), cholestane (5 α -cholestane), cholestenone (4-cholesten-3-one), coprostanol (5 β -cholestan-3 β -ol), and epicholesterol (5-cholesten-3 α -ol) were obtained from Steraloids (Newport, RI).

    Techniques:

    Effect of different sterols upon amyloid formation. Vesicles were prepared with different sterols and the zwitterionic lipid POPC. The results of thioflavin-T assays are displayed. Data are plotted for hIAPP in solution (black), LUVs containing 100 mol% POPC (red) or LUVs containing 80 mol% POPC and 20 mol% of the following sterols: cholesterol (blue), cholestanol (cyan), lathosterol (dark cyan), 7-dehydrocholesterol (yellow), epicholesterol (green), pregnenolone (pink), cholestenone (dark red), coprostanol (purple) and 5α-cholestan-3 one (grey). Experiments were conducted in 20 mM Tris·HCl, 100 mM NaCl, pH 7.4 at 25 °C with 400 μM lipid and 20 μM hIAPP.

    Journal: Biochemistry

    Article Title: Sterol Structure Strongly Modulates Membrane-IAPP Interactions

    doi: 10.1021/acs.biochem.7b01190

    Figure Lengend Snippet: Effect of different sterols upon amyloid formation. Vesicles were prepared with different sterols and the zwitterionic lipid POPC. The results of thioflavin-T assays are displayed. Data are plotted for hIAPP in solution (black), LUVs containing 100 mol% POPC (red) or LUVs containing 80 mol% POPC and 20 mol% of the following sterols: cholesterol (blue), cholestanol (cyan), lathosterol (dark cyan), 7-dehydrocholesterol (yellow), epicholesterol (green), pregnenolone (pink), cholestenone (dark red), coprostanol (purple) and 5α-cholestan-3 one (grey). Experiments were conducted in 20 mM Tris·HCl, 100 mM NaCl, pH 7.4 at 25 °C with 400 μM lipid and 20 μM hIAPP.

    Article Snippet: 5α-cholestan-3-one, cholestenone, coprostanol, and epicholesterol were obtained from Steraloids.

    Techniques:

    Effect of different sterols on membrane leakage. The time course of carboxyfluorescein leakage experiments are displayed for vesicles containing 100 mol% POPC (red), 80 mol% POPC and 20 mol% sterol: cholesterol (blue), cholestanol (cyan), lathosterol (dark cyan), 7-dehydrocholesterol (yellow), epicholesterol (green), pregnenolone (pink), cholestenone (dark red), coprostanol (purple) and 5α-cholestan-3 one (grey). Experiments were conducted in 20 mM Tris·HCl, 100 mM NaCl, pH 7.4 at 25 °C with 400 μM lipid and 20 μM hIAPP.

    Journal: Biochemistry

    Article Title: Sterol Structure Strongly Modulates Membrane-IAPP Interactions

    doi: 10.1021/acs.biochem.7b01190

    Figure Lengend Snippet: Effect of different sterols on membrane leakage. The time course of carboxyfluorescein leakage experiments are displayed for vesicles containing 100 mol% POPC (red), 80 mol% POPC and 20 mol% sterol: cholesterol (blue), cholestanol (cyan), lathosterol (dark cyan), 7-dehydrocholesterol (yellow), epicholesterol (green), pregnenolone (pink), cholestenone (dark red), coprostanol (purple) and 5α-cholestan-3 one (grey). Experiments were conducted in 20 mM Tris·HCl, 100 mM NaCl, pH 7.4 at 25 °C with 400 μM lipid and 20 μM hIAPP.

    Article Snippet: 5α-cholestan-3-one, cholestenone, coprostanol, and epicholesterol were obtained from Steraloids.

    Techniques:

    Growth curves for CG388 (A) and CG156 (B) cultured using sterol-free and sterol-supplemented glc YM. 7,22, ergosta 7,22-dienol; ERG, ergosterol; LAN, lanosterol; CAN, cholestanol; CER, cholesterol; CEN, Δ 7 -cholestenol; DES, desmosterol.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Facultative Sterol Uptake in an Ergosterol-Deficient Clinical Isolate of Candida glabrata Harboring a Missense Mutation in ERG11 and Exhibiting Cross-Resistance to Azoles and Amphotericin B

    doi: 10.1128/AAC.06253-11

    Figure Lengend Snippet: Growth curves for CG388 (A) and CG156 (B) cultured using sterol-free and sterol-supplemented glc YM. 7,22, ergosta 7,22-dienol; ERG, ergosterol; LAN, lanosterol; CAN, cholestanol; CER, cholesterol; CEN, Δ 7 -cholestenol; DES, desmosterol.

    Article Snippet: All supplements except cholestenol (Steraloids Inc.) were purchased from Sigma-Aldrich (Poole, United Kingdom).

    Techniques: Cell Culture, Gas Chromatography