chikv  (Abcam)

 
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    Name:
    Recombinant Chikungunya Virus protein
    Description:

    Catalog Number:
    AB187240
    Price:
    None
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    Structured Review

    Abcam chikv
    Predicted age-dependent seroprevalence for dengue and chikungunya. Dashed lines represent the 95% credible intervals and the bars represent the observe proportions of seropositive participants. <t>CHIKV,</t> chikungunya virus; <t>DENV,</t> dengue virus.

    https://www.bioz.com/result/chikv/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chikv - by Bioz Stars, 2021-05
    86/100 stars

    Images

    1) Product Images from "Age-dependent seroprevalence of dengue and chikungunya: inference from a cross-sectional analysis in Esmeraldas Province in coastal Ecuador"

    Article Title: Age-dependent seroprevalence of dengue and chikungunya: inference from a cross-sectional analysis in Esmeraldas Province in coastal Ecuador

    Journal: BMJ Open

    doi: 10.1136/bmjopen-2020-040735

    Predicted age-dependent seroprevalence for dengue and chikungunya. Dashed lines represent the 95% credible intervals and the bars represent the observe proportions of seropositive participants. CHIKV, chikungunya virus; DENV, dengue virus.
    Figure Legend Snippet: Predicted age-dependent seroprevalence for dengue and chikungunya. Dashed lines represent the 95% credible intervals and the bars represent the observe proportions of seropositive participants. CHIKV, chikungunya virus; DENV, dengue virus.

    Techniques Used:

    Related Articles

    Recombinant:

    Article Title: Fluorescent and electrochemical dual-mode detection of Chikungunya virus E1 protein using fluorophore-embedded and redox probe-encapsulated liposomes
    Article Snippet: Phosphate-buffered saline (PBS), FeCl2 ·4H2 O, FeCl3 ·6H2O, methanol, chloroform, acetone, and sodium citrate were purchased from Wako Pure Chemical Industries Ltd. (Japan). .. Recombinant Chikungunya virus E1 protein [ab 187240] and anti-Chikungunya virus antibody [B 1413M] [ab 130889] were purchased from Abcam Inc. (Cambridge, UK). ..

    Infection:

    Article Title: An Observational Study of Sepsis in Takeo Province Cambodia: An in-depth examination of pathogens causing severe infections
    Article Snippet: .. Serological evidence of Chikungunya virus (CHIKV) and Dengue virus (DENV) infection was detected using human anti-CHIKV IgM and IgG ELISA (Abcam, USA), and DENV IgM and IgG Capture ELISA (Panbio, USA), respectively, which were performed according to the manufacturer’s instructions. .. Serological evidence of infection with Orientia tsutsugamushi was detected using an ELISA utilizing three recombinant proteins from four strains of O . tsutsugamushi (Karp, Kato, Gilliam and TA763) [ ].

    Enzyme-linked Immunosorbent Assay:

    Article Title: An Observational Study of Sepsis in Takeo Province Cambodia: An in-depth examination of pathogens causing severe infections
    Article Snippet: .. Serological evidence of Chikungunya virus (CHIKV) and Dengue virus (DENV) infection was detected using human anti-CHIKV IgM and IgG ELISA (Abcam, USA), and DENV IgM and IgG Capture ELISA (Panbio, USA), respectively, which were performed according to the manufacturer’s instructions. .. Serological evidence of infection with Orientia tsutsugamushi was detected using an ELISA utilizing three recombinant proteins from four strains of O . tsutsugamushi (Karp, Kato, Gilliam and TA763) [ ].

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    Abcam anti chikv igm human elisa kit
    Comparison of <t>EILV/CHIKV</t> and Abcam human anti-CHIKV <t>IgM-capture</t> <t>ELISA</t> methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.
    Anti Chikv Igm Human Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti chikv igm human elisa kit/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti chikv igm human elisa kit - by Bioz Stars, 2021-05
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    93
    Abcam eilv chikv
    Comparison of <t>EILV/CHIKV</t> and Abcam human anti-CHIKV IgM-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.
    Eilv Chikv, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eilv chikv/product/Abcam
    Average 93 stars, based on 1 article reviews
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    eilv chikv - by Bioz Stars, 2021-05
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    Image Search Results


    Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgM-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgM-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Article Snippet: Of the commercial kits, Abcam’s Anti-CHIKV IgM Human ELISA kit is reported to produce comparable results to the CDC IgM ELISA [ ].

    Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Produced, Plaque Reduction Neutralization Test

    Stability of EILV/CHIKV in different buffers determined by accelerated decay at elevated temperature. Mean and standard deviations of three replicates are reported. A 2-way ANOVA with Tukey’s test was used to compare the effects of different buffer preparations on ELISA activity.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Stability of EILV/CHIKV in different buffers determined by accelerated decay at elevated temperature. Mean and standard deviations of three replicates are reported. A 2-way ANOVA with Tukey’s test was used to compare the effects of different buffer preparations on ELISA activity.

    Article Snippet: Of the commercial kits, Abcam’s Anti-CHIKV IgM Human ELISA kit is reported to produce comparable results to the CDC IgM ELISA [ ].

    Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay

    Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgG-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 80 ≤80 (n = 10), medium-positive samples had PRNT 80 160–320 (n = 10), and high-positive samples had PRNT 80 > 640 (n = 10). Means and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgG-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 80 ≤80 (n = 10), medium-positive samples had PRNT 80 160–320 (n = 10), and high-positive samples had PRNT 80 > 640 (n = 10). Means and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Article Snippet: Of the commercial kits, Abcam’s Anti-CHIKV IgM Human ELISA kit is reported to produce comparable results to the CDC IgM ELISA [ ].

    Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Produced, Plaque Reduction Neutralization Test

    Human anti-CHIKV antibody-capture ELISAs utilizing EILV/CHIKV as antigen to detect either (A) IgM or (B) IgG antibodies (both measured over a range of serum dilutions, red). Human serum samples that were antibody-positive for either DENV or VEEV but negative for CHIKV by HI were included as negative controls. Mean and standard deviations of 2 replicates are reported.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Human anti-CHIKV antibody-capture ELISAs utilizing EILV/CHIKV as antigen to detect either (A) IgM or (B) IgG antibodies (both measured over a range of serum dilutions, red). Human serum samples that were antibody-positive for either DENV or VEEV but negative for CHIKV by HI were included as negative controls. Mean and standard deviations of 2 replicates are reported.

    Article Snippet: Of the commercial kits, Abcam’s Anti-CHIKV IgM Human ELISA kit is reported to produce comparable results to the CDC IgM ELISA [ ].

    Techniques:

    Comparison of sensitivity by serum titration. Chikungunya virus (CHIKV) IgM+ samples from the Caribbean were diluted 2-fold to 1:12,800 in sample dilution buffer. The revised Abcam kit with CHIKV-specific IgM serum controls was used for the evaluation.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays

    doi: 10.4269/ajtmh.16-0013

    Figure Lengend Snippet: Comparison of sensitivity by serum titration. Chikungunya virus (CHIKV) IgM+ samples from the Caribbean were diluted 2-fold to 1:12,800 in sample dilution buffer. The revised Abcam kit with CHIKV-specific IgM serum controls was used for the evaluation.

    Article Snippet: The DENV IHPC with the CDC CHIKV IgM+ result had a negative and positive result in the Abcam test with the antigen incubated 30 minutes and overnight, respectively.

    Techniques: Titration

    Performance of CHIKV IgM detection assays compared with CDC results as the reference standard. *CDC CHIKV IHPC had negative result in test. **CDC CHIKV IHPC had positive result in test. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control; ND = not done; RT = rapid test.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays

    doi: 10.4269/ajtmh.16-0013

    Figure Lengend Snippet: Performance of CHIKV IgM detection assays compared with CDC results as the reference standard. *CDC CHIKV IHPC had negative result in test. **CDC CHIKV IHPC had positive result in test. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control; ND = not done; RT = rapid test.

    Article Snippet: The DENV IHPC with the CDC CHIKV IgM+ result had a negative and positive result in the Abcam test with the antigen incubated 30 minutes and overnight, respectively.

    Techniques: Positive Control

    Abcam CHIK MAC-ELISA performance assessment with CDC CHIKV IHPC, by lot number. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays

    doi: 10.4269/ajtmh.16-0013

    Figure Lengend Snippet: Abcam CHIK MAC-ELISA performance assessment with CDC CHIKV IHPC, by lot number. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control.

    Article Snippet: The DENV IHPC with the CDC CHIKV IgM+ result had a negative and positive result in the Abcam test with the antigen incubated 30 minutes and overnight, respectively.

    Techniques: Enzyme-linked Immunosorbent Assay, Positive Control

    Diversity of the antibody response patterns in chikungunya acute febrile versus chronic patients. ( A ) Paired analysis of CHIKV-specific plasma IgM (red circles) and IgG (blue squares) values in individual CHIKV-confirmed patients within the no antibodies, IgM-alone, or IgM and IgG antibody response patterns in the acute febrile (left, n = 133), early chronic (middle, n = 21), and late chronic (right, n = 30) phases. Within each antibody response pattern group, the patients are stratified based on increasing IgM values on the x axis. Horizontal dotted line indicates assay cutoff for IgM and IgG. The samples that were also positive for CHIKV PCR are indicated by green-filled symbols. ( B ) Evaluation of plasma CHIKV NT antibody activity in each of the patient groups that are described in A . Dotted gates were placed to further subgroup the patients based on a combination of IgM, IgG, and NT activity. NT assay limit of detection is indicated by the horizontal dotted line. The NT antibody titers were significantly different between the IgM-alone group and IgM and IgG group in the acute febrile patients. Statistical significance was calculated by unpaired Mann-Whitney U test. ( C ) Relative proportion of the patients with each of the indicated antibody response patterns shown in B among the CHIKV-confirmed patients in febrile phase (left), early chronic phase (middle), and late chronic phase (right).

    Journal: JCI Insight

    Article Title: Antibody response patterns in chikungunya febrile phase predict protection versus progression to chronic arthritis

    doi: 10.1172/jci.insight.130509

    Figure Lengend Snippet: Diversity of the antibody response patterns in chikungunya acute febrile versus chronic patients. ( A ) Paired analysis of CHIKV-specific plasma IgM (red circles) and IgG (blue squares) values in individual CHIKV-confirmed patients within the no antibodies, IgM-alone, or IgM and IgG antibody response patterns in the acute febrile (left, n = 133), early chronic (middle, n = 21), and late chronic (right, n = 30) phases. Within each antibody response pattern group, the patients are stratified based on increasing IgM values on the x axis. Horizontal dotted line indicates assay cutoff for IgM and IgG. The samples that were also positive for CHIKV PCR are indicated by green-filled symbols. ( B ) Evaluation of plasma CHIKV NT antibody activity in each of the patient groups that are described in A . Dotted gates were placed to further subgroup the patients based on a combination of IgM, IgG, and NT activity. NT assay limit of detection is indicated by the horizontal dotted line. The NT antibody titers were significantly different between the IgM-alone group and IgM and IgG group in the acute febrile patients. Statistical significance was calculated by unpaired Mann-Whitney U test. ( C ) Relative proportion of the patients with each of the indicated antibody response patterns shown in B among the CHIKV-confirmed patients in febrile phase (left), early chronic phase (middle), and late chronic phase (right).

    Article Snippet: Serology tests.CHIKV-specific IgM and IgG were detected using chikungunya IgM capture ELISA kits (Abcam ab177848) and chikungunya IgG capture ELISA kit (Abcam ab177835) following the manufacturer’s recommendations.

    Techniques: Polymerase Chain Reaction, Activity Assay, MANN-WHITNEY

    Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgM-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgM-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 50 ≤80 (n = 4), medium-positive samples had PRNT 50 160–320 (n = 4), and high-positive samples had PRNT 50 > 640 (n = 24). Mean and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Article Snippet: Statistical analysis To compare the activities of EILV/CHIKV versus CLA in indirect ELISAs, and the effects of the EILV/CHIKV and Abcam methods on absorbance values and signal-to-noise ratios, a 2-way ANOVA using a Bonferroni post-hoc test was performed.

    Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Produced, Plaque Reduction Neutralization Test

    Indirect mouse anti-CHIKV IgG ELISAs utilizing either (A) EILV/CHIKV or (B) cell-lysate antigen to detect serially diluted polyclonal anti-CHIKV MIAF (measured over a range of serum dilutions, red) or monoclonal antibody CHK-175 (expressed in ng quantities, blue). MIAF against Gamboa virus and MIS against mosquito antigens were included as negative controls. Mean and standard deviation of 2 replicates are reported.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Indirect mouse anti-CHIKV IgG ELISAs utilizing either (A) EILV/CHIKV or (B) cell-lysate antigen to detect serially diluted polyclonal anti-CHIKV MIAF (measured over a range of serum dilutions, red) or monoclonal antibody CHK-175 (expressed in ng quantities, blue). MIAF against Gamboa virus and MIS against mosquito antigens were included as negative controls. Mean and standard deviation of 2 replicates are reported.

    Article Snippet: Statistical analysis To compare the activities of EILV/CHIKV versus CLA in indirect ELISAs, and the effects of the EILV/CHIKV and Abcam methods on absorbance values and signal-to-noise ratios, a 2-way ANOVA using a Bonferroni post-hoc test was performed.

    Techniques: Standard Deviation

    Stability of EILV/CHIKV in different buffers determined by accelerated decay at elevated temperature. Mean and standard deviations of three replicates are reported. A 2-way ANOVA with Tukey’s test was used to compare the effects of different buffer preparations on ELISA activity.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Stability of EILV/CHIKV in different buffers determined by accelerated decay at elevated temperature. Mean and standard deviations of three replicates are reported. A 2-way ANOVA with Tukey’s test was used to compare the effects of different buffer preparations on ELISA activity.

    Article Snippet: Statistical analysis To compare the activities of EILV/CHIKV versus CLA in indirect ELISAs, and the effects of the EILV/CHIKV and Abcam methods on absorbance values and signal-to-noise ratios, a 2-way ANOVA using a Bonferroni post-hoc test was performed.

    Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay

    Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgG-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 80 ≤80 (n = 10), medium-positive samples had PRNT 80 160–320 (n = 10), and high-positive samples had PRNT 80 > 640 (n = 10). Means and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Comparison of EILV/CHIKV and Abcam human anti-CHIKV IgG-capture ELISA methods. (A) raw OD values or (B) signal-to-noise ratios (calculated by dividing positive sample OD values by mean negative control OD value) produced by samples of varying degrees of seropositivity were compared. Low-positive samples had PRNT 80 ≤80 (n = 10), medium-positive samples had PRNT 80 160–320 (n = 10), and high-positive samples had PRNT 80 > 640 (n = 10). Means and standard deviations are reported. A 2-way ANOVA with Tukey’s test was used to compare absorbance values and signal-to-noise ratios within each ELISA method. 2-way ANOVA with Bonferroni’s test was used to compare absorbance values and signal-to-noise ratios between each ELISA method.

    Article Snippet: Statistical analysis To compare the activities of EILV/CHIKV versus CLA in indirect ELISAs, and the effects of the EILV/CHIKV and Abcam methods on absorbance values and signal-to-noise ratios, a 2-way ANOVA using a Bonferroni post-hoc test was performed.

    Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Produced, Plaque Reduction Neutralization Test

    Safety of EILV/CHIKV in the newborn mouse model of neurovirulence. (A) Survival of infant mice infected IC with EILV/CHIKV, live-attenuated vaccine strain 181/25, or PBS. (B) Replication of EILV/CHIKV or strain 181/25 in newborn mouse brain tissue.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Safety of EILV/CHIKV in the newborn mouse model of neurovirulence. (A) Survival of infant mice infected IC with EILV/CHIKV, live-attenuated vaccine strain 181/25, or PBS. (B) Replication of EILV/CHIKV or strain 181/25 in newborn mouse brain tissue.

    Article Snippet: Statistical analysis To compare the activities of EILV/CHIKV versus CLA in indirect ELISAs, and the effects of the EILV/CHIKV and Abcam methods on absorbance values and signal-to-noise ratios, a 2-way ANOVA using a Bonferroni post-hoc test was performed.

    Techniques: Mouse Assay, Infection

    Human anti-CHIKV antibody-capture ELISAs utilizing EILV/CHIKV as antigen to detect either (A) IgM or (B) IgG antibodies (both measured over a range of serum dilutions, red). Human serum samples that were antibody-positive for either DENV or VEEV but negative for CHIKV by HI were included as negative controls. Mean and standard deviations of 2 replicates are reported.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

    doi: 10.1371/journal.pntd.0004119

    Figure Lengend Snippet: Human anti-CHIKV antibody-capture ELISAs utilizing EILV/CHIKV as antigen to detect either (A) IgM or (B) IgG antibodies (both measured over a range of serum dilutions, red). Human serum samples that were antibody-positive for either DENV or VEEV but negative for CHIKV by HI were included as negative controls. Mean and standard deviations of 2 replicates are reported.

    Article Snippet: Statistical analysis To compare the activities of EILV/CHIKV versus CLA in indirect ELISAs, and the effects of the EILV/CHIKV and Abcam methods on absorbance values and signal-to-noise ratios, a 2-way ANOVA using a Bonferroni post-hoc test was performed.

    Techniques: