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CTK Biotech chikungunya virus
Chikungunya Virus, supplied by CTK Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chikungunya virus/product/CTK Biotech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
chikungunya virus - by Bioz Stars, 2021-05
86/100 stars

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Recombinant:

Article Title: The Immune Response to Eastern Equine Encephalitis Virus Acquired Through Organ Transplantation
Article Snippet: .. Memory B Cell ELISpotELISpot assays for memory B cells producing IgG to EEEV antigen, normal suckling mouse brain antigen, or recombinant chikungunya virus glycoprotein (CTK Biotech, #A2321) were performed as previously described under BSL 2+ conditions ( ). ..

Article Title: Use of Bead-Based Serologic Assay to Evaluate Chikungunya Virus Epidemic, Haiti
Article Snippet: Carboxyl groups on the surface of specifically classified spectral polystyrene microspheres (BioPlex microbeads; Bio-Rad, Hercules, CA, USA) were converted to reactive esters by using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide method (Calbiochem, Woburn, MA, USA). .. The recombinant CHIKV wild-type and mutant (A226V) envelope 1 (E1) antigens (CTK Biotech, San Diego, CA, USA), 7.5 μg each, were covalently linked to 1 mL (12.5 × 106 microbeads) of activated microspheres by amide bonds by using phosphate-buffered saline (pH 7.2). .. To confirm the coupling reaction, the serum we tested was previously found to be highly reactive to the antigens; these showed high median fluorescence intensity (MFI) minus background (MFI-bg) values, indicating appropriate antigen coupling to the microbeads.

Article Title: Use of Bead-Based Serologic Assay to Evaluate Chikungunya Virus Epidemic, Haiti
Article Snippet: Carboxyl groups on the surface of specifically classified spectral polystyrene microspheres (BioPlex microbeads; Bio-Rad, Hercules, CA, USA) were converted to reactive esters by using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide method (Calbiochem, Woburn, MA, USA). .. The recombinant CHIKV wild-type and mutant (A226V) envelope 1 (E1) antigens (CTK Biotech, San Diego, CA, USA), 7.5 μg each, were covalently linked to 1 mL (12.5 × 106 microbeads) of activated microspheres by amide bonds by using phosphate-buffered saline (pH 7.2). .. To confirm the coupling reaction, the serum we tested was previously found to be highly reactive to the antigens; these showed high median fluorescence intensity (MFI) minus background (MFI-bg) values, indicating appropriate antigen coupling to the microbeads.

Article Title: Serological Data Shows Low Levels of Chikungunya Exposure in Senegalese Nomadic Pastoralists
Article Snippet: CHIKV E1 Antigen Coupling to Beads Carboxyl groups on the surface of spectrally classified-magnetic polystyrene microspheres (MagPlex Beads; Luminex Corporation, Austin, TX, USA) were converted to reactive esters using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide method (Calbiochem, Woburn, MA, USA). .. Then, 8.7 µg of recombinant CHIKV E1 antigen (CTK Biotech, San Diego, CA, USA) was covalently linked to microspheres (12.5 million) by covalent amide bonds in phosphate buffered saline (PBS), pH 7.2, through the reaction of amino groups on the antigen and the created ester groups on the beads. ..

Article Title: Measuring Haitian children's exposure to chikungunya, dengue and malaria
Article Snippet: .. Antigens We used one recombinant chikungunya virus antigen – that is, mutant A226V envelope 1 antigen (CTK Biotech, San Diego, USA) – two dengue virus antigens – propagated using a eukaryotic plasmid vector that expressed the premembrane/membrane and envelope proteins that self-assemble into two different non-infectious virus-like particles known as DENV-2 and DENV-3 , – and three recombinant antigens based on merozoite surface protein 1 of P. falciparum . ..

Mutagenesis:

Article Title: Use of Bead-Based Serologic Assay to Evaluate Chikungunya Virus Epidemic, Haiti
Article Snippet: Carboxyl groups on the surface of specifically classified spectral polystyrene microspheres (BioPlex microbeads; Bio-Rad, Hercules, CA, USA) were converted to reactive esters by using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide method (Calbiochem, Woburn, MA, USA). .. The recombinant CHIKV wild-type and mutant (A226V) envelope 1 (E1) antigens (CTK Biotech, San Diego, CA, USA), 7.5 μg each, were covalently linked to 1 mL (12.5 × 106 microbeads) of activated microspheres by amide bonds by using phosphate-buffered saline (pH 7.2). .. To confirm the coupling reaction, the serum we tested was previously found to be highly reactive to the antigens; these showed high median fluorescence intensity (MFI) minus background (MFI-bg) values, indicating appropriate antigen coupling to the microbeads.

Article Title: Use of Bead-Based Serologic Assay to Evaluate Chikungunya Virus Epidemic, Haiti
Article Snippet: Carboxyl groups on the surface of specifically classified spectral polystyrene microspheres (BioPlex microbeads; Bio-Rad, Hercules, CA, USA) were converted to reactive esters by using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide method (Calbiochem, Woburn, MA, USA). .. The recombinant CHIKV wild-type and mutant (A226V) envelope 1 (E1) antigens (CTK Biotech, San Diego, CA, USA), 7.5 μg each, were covalently linked to 1 mL (12.5 × 106 microbeads) of activated microspheres by amide bonds by using phosphate-buffered saline (pH 7.2). .. To confirm the coupling reaction, the serum we tested was previously found to be highly reactive to the antigens; these showed high median fluorescence intensity (MFI) minus background (MFI-bg) values, indicating appropriate antigen coupling to the microbeads.

Article Title: Measuring Haitian children's exposure to chikungunya, dengue and malaria
Article Snippet: .. Antigens We used one recombinant chikungunya virus antigen – that is, mutant A226V envelope 1 antigen (CTK Biotech, San Diego, USA) – two dengue virus antigens – propagated using a eukaryotic plasmid vector that expressed the premembrane/membrane and envelope proteins that self-assemble into two different non-infectious virus-like particles known as DENV-2 and DENV-3 , – and three recombinant antigens based on merozoite surface protein 1 of P. falciparum . ..

Reverse Transcription Polymerase Chain Reaction:

Article Title: A clinical report on mixed infection of malaria, dengue and chikungunya from New Delhi, India
Article Snippet: Blood sample (acute phase) was collected and processed for routine laboratory investigations including haemogram, malarial antigen (Malaria Antigen Kit SD BIOLINE, Alere, Korea), peripheral smear for malaria parasite and Widal test for typhoid fever. .. Additionally, RT-PCR and IgM lateral flow immunoassay for dengue virus (OnSite Dengue IgG/IgM 3.0 Combo Rapid Test, CTK Biotech) and chikungunya virus [OnSite Chikungunya IgM Combo (CTK) Rapid test, CTK Biotech] was also performed keeping in view of co-circulation of these viruses during that time in this region. ..

Flow Cytometry:

Article Title: A clinical report on mixed infection of malaria, dengue and chikungunya from New Delhi, India
Article Snippet: Blood sample (acute phase) was collected and processed for routine laboratory investigations including haemogram, malarial antigen (Malaria Antigen Kit SD BIOLINE, Alere, Korea), peripheral smear for malaria parasite and Widal test for typhoid fever. .. Additionally, RT-PCR and IgM lateral flow immunoassay for dengue virus (OnSite Dengue IgG/IgM 3.0 Combo Rapid Test, CTK Biotech) and chikungunya virus [OnSite Chikungunya IgM Combo (CTK) Rapid test, CTK Biotech] was also performed keeping in view of co-circulation of these viruses during that time in this region. ..

Plasmid Preparation:

Article Title: Measuring Haitian children's exposure to chikungunya, dengue and malaria
Article Snippet: .. Antigens We used one recombinant chikungunya virus antigen – that is, mutant A226V envelope 1 antigen (CTK Biotech, San Diego, USA) – two dengue virus antigens – propagated using a eukaryotic plasmid vector that expressed the premembrane/membrane and envelope proteins that self-assemble into two different non-infectious virus-like particles known as DENV-2 and DENV-3 , – and three recombinant antigens based on merozoite surface protein 1 of P. falciparum . ..

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