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EUROIMMUN chikungunya virus chikv
Chikungunya Virus Chikv, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Flow Cytometry:

Article Title: Evaluation of Chikungunya Diagnostic Assays: Differences in Sensitivity of Serology Assays in Two Independent Outbreaks
Article Snippet: .. Using sera collected in outbreaks caused by two variants of Chikungunya virus (A226 and 226V), we tested 2 commercial IgM tests (CTK lateral flow rapid test and EUROIMMUN IFA) alongside our in-house IgM assays (using both variants of the virus). .. Sensitivities of 2 published PCR protocols were also evaluated based on RNA standards derived from cell-cultured viruses.

Immunofluorescence:

Article Title: Evaluation of Chikungunya Diagnostic Assays: Differences in Sensitivity of Serology Assays in Two Independent Outbreaks
Article Snippet: .. Using sera collected in outbreaks caused by two variants of Chikungunya virus (A226 and 226V), we tested 2 commercial IgM tests (CTK lateral flow rapid test and EUROIMMUN IFA) alongside our in-house IgM assays (using both variants of the virus). .. Sensitivities of 2 published PCR protocols were also evaluated based on RNA standards derived from cell-cultured viruses.

other:

Article Title: Thrombotic thrombocytopenic purpura associated with dengue and chikungunya virus coinfection: case report during an epidemic period
Article Snippet: This case report describes a severe hematological complication associated with emerging arboviruses, such as chikungunya virus, which is endemic in Brazil in a coinfection with dengue virus.

Enzyme-linked Immunosorbent Assay:

Article Title: Central and peripheral nervous system involvement caused by Zika and chikungunya coinfection
Article Snippet: .. An IgM ELISA test specific for chikungunya virus (EuroImmun, Lübeck, Germany) was also positive in the CSF; however, the results of IgM ELISA for Zika and dengue viruses were both negative on CSF. ..

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    EUROIMMUN anti chikv elisa kit evaluations
    Abcam CHIK <t>MAC-ELISA</t> performance assessment with CDC <t>CHIKV</t> IHPC, by lot number. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control.
    Anti Chikv Elisa Kit Evaluations, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti chikv elisa kit evaluations/product/EUROIMMUN
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti chikv elisa kit evaluations - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    EUROIMMUN chikv igg
    ZIKV seroprevalence and reported cases. (A) ZIKV, <t>CHIKV,</t> and DENV seroprevalence in parturients. Non-microcephaly pregnancies (PRG) ( n = 257 for ZIKV <t>IgG</t> and CHIKV IgG and n = 69 for DENV IgG); microcephaly pregnancies (MC) ( n = 19 for ZIKV IgG and CHIKV IgG and n = 0 for DENV IgG). (B) ZIKV, CHIKV, and DENV seroprevalence in HIV-positive patients from 2013 ( n = 96 for ZIKV IgG and CHIKV IgG and n = 52 for DENV IgG), 2014 ( n = 89 for ZIKV IgG and CHIKV IgG and n = 57 for DENV IgG), 2015 ( n = 92 for ZIKV IgG and CHIKV IgG and n = 46 for DENV IgG), and 2016 ( n = 263 for ZIKV IgG and CHIKV IgG and n = 110 for DENV IgG). (C) ZIKV, CHIKV, and DENV seroprevalence in tuberculosis patients (TBC) ( n = 55 for ZIKV IgG and CHIKV IgG and n = 8 for DENV IgG) and university employees (UNI) ( n = 39 for ZIKV IgG and CHIKV IgG and n = 20 for DENV IgG). (D) ZIKV, CHIKV, and DENV seroprevalence in all 633 samples from 2016. The bars in panels A to D depict 95% confidence intervals. (E) Seroprevalence per age group for ZIKV IgG, CHIKV, and DENV in 633 samples from 2016. (F) Reported Brazilian cases of acute exanthematic disease in Salvador and Bahia until epidemiological week 22 in 2017. The months are indicated by capital first letter.
    Chikv Igg, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chikv igg/product/EUROIMMUN
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chikv igg - by Bioz Stars, 2021-05
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    86
    EUROIMMUN igm igg indirect immunofluorescence assay ifa
    Proportions of positive chikungunya virus-specific RT-PCR, <t>IgM</t> and <t>IgG</t> antibodies (IFA) per day of sampling post symptom onset *. * Note: exact date of sampling was available for 261 patients with confirmed CHIKV infection.
    Igm Igg Indirect Immunofluorescence Assay Ifa, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igm igg indirect immunofluorescence assay ifa/product/EUROIMMUN
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igm igg indirect immunofluorescence assay ifa - by Bioz Stars, 2021-05
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    EUROIMMUN chikv igg igm elisa
    Study algorithm to identify acute chikungunya infection. *DENV <t>IgM/IgG,</t> NS1, RT-PCR; ** <t>CHIKV</t> IgM/IgG, RT-PCR.
    Chikv Igg Igm Elisa, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chikv igg igm elisa/product/EUROIMMUN
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chikv igg igm elisa - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    Abcam CHIK MAC-ELISA performance assessment with CDC CHIKV IHPC, by lot number. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays

    doi: 10.4269/ajtmh.16-0013

    Figure Lengend Snippet: Abcam CHIK MAC-ELISA performance assessment with CDC CHIKV IHPC, by lot number. CDC = Centers for Disease Control and Prevention; CHIKV = chikungunya virus; IHPC = in-house positive control.

    Article Snippet: Serum samples selected for Euroimmun Anti-CHIKV ELISA kit evaluations were previously tested by an in-house CHIKV MAC-ELISA based on the CDC MAC-ELISA method described above, with the exceptions that samples were tested in duplicate and the assay used tissue culture–derived antigen produced at NML.

    Techniques: Enzyme-linked Immunosorbent Assay, Positive Control

    ZIKV seroprevalence and reported cases. (A) ZIKV, CHIKV, and DENV seroprevalence in parturients. Non-microcephaly pregnancies (PRG) ( n = 257 for ZIKV IgG and CHIKV IgG and n = 69 for DENV IgG); microcephaly pregnancies (MC) ( n = 19 for ZIKV IgG and CHIKV IgG and n = 0 for DENV IgG). (B) ZIKV, CHIKV, and DENV seroprevalence in HIV-positive patients from 2013 ( n = 96 for ZIKV IgG and CHIKV IgG and n = 52 for DENV IgG), 2014 ( n = 89 for ZIKV IgG and CHIKV IgG and n = 57 for DENV IgG), 2015 ( n = 92 for ZIKV IgG and CHIKV IgG and n = 46 for DENV IgG), and 2016 ( n = 263 for ZIKV IgG and CHIKV IgG and n = 110 for DENV IgG). (C) ZIKV, CHIKV, and DENV seroprevalence in tuberculosis patients (TBC) ( n = 55 for ZIKV IgG and CHIKV IgG and n = 8 for DENV IgG) and university employees (UNI) ( n = 39 for ZIKV IgG and CHIKV IgG and n = 20 for DENV IgG). (D) ZIKV, CHIKV, and DENV seroprevalence in all 633 samples from 2016. The bars in panels A to D depict 95% confidence intervals. (E) Seroprevalence per age group for ZIKV IgG, CHIKV, and DENV in 633 samples from 2016. (F) Reported Brazilian cases of acute exanthematic disease in Salvador and Bahia until epidemiological week 22 in 2017. The months are indicated by capital first letter.

    Journal: mBio

    Article Title: High Zika Virus Seroprevalence in Salvador, Northeastern Brazil Limits the Potential for Further Outbreaks

    doi: 10.1128/mBio.01390-17

    Figure Lengend Snippet: ZIKV seroprevalence and reported cases. (A) ZIKV, CHIKV, and DENV seroprevalence in parturients. Non-microcephaly pregnancies (PRG) ( n = 257 for ZIKV IgG and CHIKV IgG and n = 69 for DENV IgG); microcephaly pregnancies (MC) ( n = 19 for ZIKV IgG and CHIKV IgG and n = 0 for DENV IgG). (B) ZIKV, CHIKV, and DENV seroprevalence in HIV-positive patients from 2013 ( n = 96 for ZIKV IgG and CHIKV IgG and n = 52 for DENV IgG), 2014 ( n = 89 for ZIKV IgG and CHIKV IgG and n = 57 for DENV IgG), 2015 ( n = 92 for ZIKV IgG and CHIKV IgG and n = 46 for DENV IgG), and 2016 ( n = 263 for ZIKV IgG and CHIKV IgG and n = 110 for DENV IgG). (C) ZIKV, CHIKV, and DENV seroprevalence in tuberculosis patients (TBC) ( n = 55 for ZIKV IgG and CHIKV IgG and n = 8 for DENV IgG) and university employees (UNI) ( n = 39 for ZIKV IgG and CHIKV IgG and n = 20 for DENV IgG). (D) ZIKV, CHIKV, and DENV seroprevalence in all 633 samples from 2016. The bars in panels A to D depict 95% confidence intervals. (E) Seroprevalence per age group for ZIKV IgG, CHIKV, and DENV in 633 samples from 2016. (F) Reported Brazilian cases of acute exanthematic disease in Salvador and Bahia until epidemiological week 22 in 2017. The months are indicated by capital first letter.

    Article Snippet: Serological testing was performed by using enzyme-linked immunosorbent assays (ELISAs) for ZIKV IgM/IgG (Euroimmun, Lübeck, Germany) (NS1 antigen), DENV IgG (Euroimmun) (full virus lysates), and CHIKV IgG (Euroimmun) (recombinant structural protein) according to the manufacturer’s instructions.

    Techniques:

    Proportions of positive chikungunya virus-specific RT-PCR, IgM and IgG antibodies (IFA) per day of sampling post symptom onset *. * Note: exact date of sampling was available for 261 patients with confirmed CHIKV infection.

    Journal: PLoS ONE

    Article Title: Chikungunya virus infection in Aruba: Diagnosis, clinical features and predictors of post-chikungunya chronic polyarthralgia

    doi: 10.1371/journal.pone.0196630

    Figure Lengend Snippet: Proportions of positive chikungunya virus-specific RT-PCR, IgM and IgG antibodies (IFA) per day of sampling post symptom onset *. * Note: exact date of sampling was available for 261 patients with confirmed CHIKV infection.

    Article Snippet: Diagnostics Acute CHIKV infection in our study was defined by either or both positive results of CHIKV-specific IgM in an IgM/IgG indirect immunofluorescence assay (IFA) (Anti-CHIKV IIFT IgM/IgG, Euroimmun, Lübeck, Germany) according to manufacturer’s instructions, and CHIKV-specific real-time reverse-transcription polymerase chain reaction (RT-PCR).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Sampling, Infection

    Study design. * Acute phase sera refers to all available sera collected within 10 days of symptom onset. § Positive IgM: Immunoglobulin M (IgM) detected in indirect immunofluorescence assay (IFA) (Anti-CHIKV IIFT IgM/IgG, Euroimmun, Lübeck, Germany) according to manufacturer’s instructions. §§ positive RT-PCR: CHIKV RNA detected in a RT-PCR targeting the NSP-1 gene using primers and probes that detect CHIKV strain (LR2006_OPY_1, Indian Ocean Islands and the African prototype (S27, general strain)).

    Journal: PLoS ONE

    Article Title: Chikungunya virus infection in Aruba: Diagnosis, clinical features and predictors of post-chikungunya chronic polyarthralgia

    doi: 10.1371/journal.pone.0196630

    Figure Lengend Snippet: Study design. * Acute phase sera refers to all available sera collected within 10 days of symptom onset. § Positive IgM: Immunoglobulin M (IgM) detected in indirect immunofluorescence assay (IFA) (Anti-CHIKV IIFT IgM/IgG, Euroimmun, Lübeck, Germany) according to manufacturer’s instructions. §§ positive RT-PCR: CHIKV RNA detected in a RT-PCR targeting the NSP-1 gene using primers and probes that detect CHIKV strain (LR2006_OPY_1, Indian Ocean Islands and the African prototype (S27, general strain)).

    Article Snippet: Diagnostics Acute CHIKV infection in our study was defined by either or both positive results of CHIKV-specific IgM in an IgM/IgG indirect immunofluorescence assay (IFA) (Anti-CHIKV IIFT IgM/IgG, Euroimmun, Lübeck, Germany) according to manufacturer’s instructions, and CHIKV-specific real-time reverse-transcription polymerase chain reaction (RT-PCR).

    Techniques: Immunofluorescence, Reverse Transcription Polymerase Chain Reaction

    Study algorithm to identify acute chikungunya infection. *DENV IgM/IgG, NS1, RT-PCR; ** CHIKV IgM/IgG, RT-PCR.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Chikungunya in Indonesia: Epidemiology and diagnostic challenges

    doi: 10.1371/journal.pntd.0008355

    Figure Lengend Snippet: Study algorithm to identify acute chikungunya infection. *DENV IgM/IgG, NS1, RT-PCR; ** CHIKV IgM/IgG, RT-PCR.

    Article Snippet: If positive, paired acute and convalescent samples were tested simultaneously by CHIKV IgG/IgM ELISA (Euroimmun Ag, Lubeck, Germany), and acute plasma samples were further tested by real-time reverse transcriptase PCR (rRT-PCR) [ ].

    Techniques: Infection, Reverse Transcription Polymerase Chain Reaction

    The distribution of Chikungunya virus exposure in Indonesia. Acute CHIKV infection (ACI) : CHIKV RNA was detected by rRT-PCR and/or sero-conversion or four-fold increase in OD ratio of IgM and IgG between paired samples was observed; Previous infection : no evidence of ACI, IgG was positive in acute specimens; No exposure of CHIKV : IgG was negative in convalescent specimens. Map source Wikimedia Commons Atlas of the World [Atlas of Indonesia] . Available from: https://commons.wikimedia.org/wiki/Atlas_of_Indonesia#/media/File:Map_of_Indonesia_Demis.png [Accessed 21 October 2019].

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Chikungunya in Indonesia: Epidemiology and diagnostic challenges

    doi: 10.1371/journal.pntd.0008355

    Figure Lengend Snippet: The distribution of Chikungunya virus exposure in Indonesia. Acute CHIKV infection (ACI) : CHIKV RNA was detected by rRT-PCR and/or sero-conversion or four-fold increase in OD ratio of IgM and IgG between paired samples was observed; Previous infection : no evidence of ACI, IgG was positive in acute specimens; No exposure of CHIKV : IgG was negative in convalescent specimens. Map source Wikimedia Commons Atlas of the World [Atlas of Indonesia] . Available from: https://commons.wikimedia.org/wiki/Atlas_of_Indonesia#/media/File:Map_of_Indonesia_Demis.png [Accessed 21 October 2019].

    Article Snippet: If positive, paired acute and convalescent samples were tested simultaneously by CHIKV IgG/IgM ELISA (Euroimmun Ag, Lubeck, Germany), and acute plasma samples were further tested by real-time reverse transcriptase PCR (rRT-PCR) [ ].

    Techniques: Infection, Quantitative RT-PCR