chikungunya virus chikv immunofluorescence assay  (EUROIMMUN)

 
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    EUROIMMUN chikungunya virus chikv immunofluorescence assay
    Chikungunya Virus Chikv Immunofluorescence Assay, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chikungunya virus chikv immunofluorescence assay/product/EUROIMMUN
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chikungunya virus chikv immunofluorescence assay - by Bioz Stars, 2021-05
    86/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Zika Virus Screening among Spanish Team Members After 2016 Rio de Janeiro, Brazil, Olympic Games
    Article Snippet: .. A total of 10 mL of blood was drawn from each participant, and serologic tests for Zika virus (immunofluorescence antibody assay; EUROIMMUN, Luebeck, Germany), dengue virus (ELISA; Panbio, Kyonggi-do, Republic of Korea), and chikungunya virus (immunofluorescence assay; EUROIMMUN) were conducted at the Instituto de Salud Carlos III (Spanish National Reference Laboratory, Madrid, Spain). ..

    Article Title: Zika Virus in Israeli Travelers: Emergence of Asia as a Major Source of Infection
    Article Snippet: Zika virus reverse transcription polymerase chain reaction ( RT - PCR ) methods were performed as described previously. .. – Serology was performed on sera by using an enzyme-linked immunosorbent assay (ELISA) IgM and IgG kit (Euroimmun AG, Lübeck, Germany), which detects antibodies against ZIKV nonstructural protein 1 (NS-1) protein and is considered very specific for ZIKV infection., Evaluation of other arboviral infections was achieved by performing serology tests against dengue virus (DENV) (Panbio NS1 early antigen, IgM and IgG ELISA; Alere (Abbott), Waltham, MA), West Nile virus (IgM and IgG ELISA Focus Diagnostics, Cypress, CA), and Chikungunya virus (CHIKV) (Immunofluorescence assay, [Euroimmun AG]). .. Positive and equivocal ZIKV serology was confirmed by neutralization assay (NA), as described elsewhere.

    Article Title: Diagnosis of Zika Virus Infection by Peptide Array and Enzyme-Linked Immunosorbent Assay
    Article Snippet: .. Convalescent-phase CHIKV sera were tested for anti-CHIKV antibodies with Euroimmun anti-chikungunya virus immunofluorescent assay (IFA) (IgG). .. Five banked WNV early-convalescent-phase sera after natural infection were also used ( ).

    Article Title: On the Home Front: Specialised Reference Testing for Dengue in the Australasian Region
    Article Snippet: Thus, by 2015, when explosive ZIKV outbreaks commenced in Brazil and later intensified throughout the Americas [ ], PHV was equipped to monitor and provide laboratory assessment of viraemic travellers suspected of ZIKV infection. .. ESR has provided DENV serological testing (Panbio, Alere, Australia) since 2008 and introduced a CHIKV immunofluorescence assay (IFA) (Euroimmun, Lübeck, Germany) and a ZIKV enzyme-linked immunosorbent assay (ELISA) (Euroimmun, Lübeck, Germany) in 2015 and 2016, respectively. .. Molecular RT-rtPCR diagnostic platforms were introduced for DENV 1–4 (2015), CHIKV (2015), and ZIKV (2014) in response to increased co-circulation of these viruses in the Pacific region combined with the escalating number of imported cases.

    Article Title: Zika Virus in Israeli Travelers: Emergence of Asia as a Major Source of Infection
    Article Snippet: Zika virus reverse transcription polymerase chain reaction ( RT - PCR ) methods were performed as described previously. .. – Serology was performed on sera by using an enzyme-linked immunosorbent assay (ELISA) IgM and IgG kit (Euroimmun AG, Lübeck, Germany), which detects antibodies against ZIKV nonstructural protein 1 (NS-1) protein and is considered very specific for ZIKV infection., Evaluation of other arboviral infections was achieved by performing serology tests against dengue virus (DENV) (Panbio NS1 early antigen, IgM and IgG ELISA; Alere (Abbott), Waltham, MA), West Nile virus (IgM and IgG ELISA Focus Diagnostics, Cypress, CA), and Chikungunya virus (CHIKV) (Immunofluorescence assay, [Euroimmun AG]). .. Positive and equivocal ZIKV serology was confirmed by neutralization assay (NA), as described elsewhere.

    Article Title: Antibody-based assay discriminates Zika virus infection from other flaviviruses
    Article Snippet: .. The diagnostic assessment for DENV, ZIKV, CHIKV, WNV, and YFV included the following: detection of anti-DENV IgM and IgG antibodies in serum samples (using dengue virus IgM Capture DxSelect and dengue virus IgG DxSelect, Focus Diagnostics, United States), detection of ZIKV IgM and IgG antibodies [anti-Zika virus ELISA (IgM) and anti-Zika virus ELISA (IgG); Euroimmun], identification of CHIKV IgM and IgG using specific immunofluorescence tests [anti-chikungunya virus IFA (IgG), anti-chikungunya virus IFA (IgM); Euroimmun], detection of WNV IgM and IgG using specific tests [NovaLisaTM Dengue IgM and IgG ELISAs (NovaTec Immunodiagnostic GmbH); and WNV IgM Capture DxSelect and WNV IgG DxSelect (Focus Diagnostics)], and detection of YFV IgM and IgG with anti-yellow fever virus IFA (IgG) and anti-yellow fever virus IFA (IgM; Euroimmun). .. Serology results were confirmed by specific viruses neutralization assays ( ).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Zika Virus Screening among Spanish Team Members After 2016 Rio de Janeiro, Brazil, Olympic Games
    Article Snippet: .. A total of 10 mL of blood was drawn from each participant, and serologic tests for Zika virus (immunofluorescence antibody assay; EUROIMMUN, Luebeck, Germany), dengue virus (ELISA; Panbio, Kyonggi-do, Republic of Korea), and chikungunya virus (immunofluorescence assay; EUROIMMUN) were conducted at the Instituto de Salud Carlos III (Spanish National Reference Laboratory, Madrid, Spain). ..

    Article Title: Zika Virus in Israeli Travelers: Emergence of Asia as a Major Source of Infection
    Article Snippet: Zika virus reverse transcription polymerase chain reaction ( RT - PCR ) methods were performed as described previously. .. – Serology was performed on sera by using an enzyme-linked immunosorbent assay (ELISA) IgM and IgG kit (Euroimmun AG, Lübeck, Germany), which detects antibodies against ZIKV nonstructural protein 1 (NS-1) protein and is considered very specific for ZIKV infection., Evaluation of other arboviral infections was achieved by performing serology tests against dengue virus (DENV) (Panbio NS1 early antigen, IgM and IgG ELISA; Alere (Abbott), Waltham, MA), West Nile virus (IgM and IgG ELISA Focus Diagnostics, Cypress, CA), and Chikungunya virus (CHIKV) (Immunofluorescence assay, [Euroimmun AG]). .. Positive and equivocal ZIKV serology was confirmed by neutralization assay (NA), as described elsewhere.

    Article Title: On the Home Front: Specialised Reference Testing for Dengue in the Australasian Region
    Article Snippet: Thus, by 2015, when explosive ZIKV outbreaks commenced in Brazil and later intensified throughout the Americas [ ], PHV was equipped to monitor and provide laboratory assessment of viraemic travellers suspected of ZIKV infection. .. ESR has provided DENV serological testing (Panbio, Alere, Australia) since 2008 and introduced a CHIKV immunofluorescence assay (IFA) (Euroimmun, Lübeck, Germany) and a ZIKV enzyme-linked immunosorbent assay (ELISA) (Euroimmun, Lübeck, Germany) in 2015 and 2016, respectively. .. Molecular RT-rtPCR diagnostic platforms were introduced for DENV 1–4 (2015), CHIKV (2015), and ZIKV (2014) in response to increased co-circulation of these viruses in the Pacific region combined with the escalating number of imported cases.

    Article Title: Zika Virus in Israeli Travelers: Emergence of Asia as a Major Source of Infection
    Article Snippet: Zika virus reverse transcription polymerase chain reaction ( RT - PCR ) methods were performed as described previously. .. – Serology was performed on sera by using an enzyme-linked immunosorbent assay (ELISA) IgM and IgG kit (Euroimmun AG, Lübeck, Germany), which detects antibodies against ZIKV nonstructural protein 1 (NS-1) protein and is considered very specific for ZIKV infection., Evaluation of other arboviral infections was achieved by performing serology tests against dengue virus (DENV) (Panbio NS1 early antigen, IgM and IgG ELISA; Alere (Abbott), Waltham, MA), West Nile virus (IgM and IgG ELISA Focus Diagnostics, Cypress, CA), and Chikungunya virus (CHIKV) (Immunofluorescence assay, [Euroimmun AG]). .. Positive and equivocal ZIKV serology was confirmed by neutralization assay (NA), as described elsewhere.

    Article Title: Antibody-based assay discriminates Zika virus infection from other flaviviruses
    Article Snippet: .. The diagnostic assessment for DENV, ZIKV, CHIKV, WNV, and YFV included the following: detection of anti-DENV IgM and IgG antibodies in serum samples (using dengue virus IgM Capture DxSelect and dengue virus IgG DxSelect, Focus Diagnostics, United States), detection of ZIKV IgM and IgG antibodies [anti-Zika virus ELISA (IgM) and anti-Zika virus ELISA (IgG); Euroimmun], identification of CHIKV IgM and IgG using specific immunofluorescence tests [anti-chikungunya virus IFA (IgG), anti-chikungunya virus IFA (IgM); Euroimmun], detection of WNV IgM and IgG using specific tests [NovaLisaTM Dengue IgM and IgG ELISAs (NovaTec Immunodiagnostic GmbH); and WNV IgM Capture DxSelect and WNV IgG DxSelect (Focus Diagnostics)], and detection of YFV IgM and IgG with anti-yellow fever virus IFA (IgG) and anti-yellow fever virus IFA (IgM; Euroimmun). .. Serology results were confirmed by specific viruses neutralization assays ( ).

    Infection:

    Article Title: Zika Virus in Israeli Travelers: Emergence of Asia as a Major Source of Infection
    Article Snippet: Zika virus reverse transcription polymerase chain reaction ( RT - PCR ) methods were performed as described previously. .. – Serology was performed on sera by using an enzyme-linked immunosorbent assay (ELISA) IgM and IgG kit (Euroimmun AG, Lübeck, Germany), which detects antibodies against ZIKV nonstructural protein 1 (NS-1) protein and is considered very specific for ZIKV infection., Evaluation of other arboviral infections was achieved by performing serology tests against dengue virus (DENV) (Panbio NS1 early antigen, IgM and IgG ELISA; Alere (Abbott), Waltham, MA), West Nile virus (IgM and IgG ELISA Focus Diagnostics, Cypress, CA), and Chikungunya virus (CHIKV) (Immunofluorescence assay, [Euroimmun AG]). .. Positive and equivocal ZIKV serology was confirmed by neutralization assay (NA), as described elsewhere.

    Article Title: Zika Virus in Israeli Travelers: Emergence of Asia as a Major Source of Infection
    Article Snippet: Zika virus reverse transcription polymerase chain reaction ( RT - PCR ) methods were performed as described previously. .. – Serology was performed on sera by using an enzyme-linked immunosorbent assay (ELISA) IgM and IgG kit (Euroimmun AG, Lübeck, Germany), which detects antibodies against ZIKV nonstructural protein 1 (NS-1) protein and is considered very specific for ZIKV infection., Evaluation of other arboviral infections was achieved by performing serology tests against dengue virus (DENV) (Panbio NS1 early antigen, IgM and IgG ELISA; Alere (Abbott), Waltham, MA), West Nile virus (IgM and IgG ELISA Focus Diagnostics, Cypress, CA), and Chikungunya virus (CHIKV) (Immunofluorescence assay, [Euroimmun AG]). .. Positive and equivocal ZIKV serology was confirmed by neutralization assay (NA), as described elsewhere.

    Electron Paramagnetic Resonance:

    Article Title: On the Home Front: Specialised Reference Testing for Dengue in the Australasian Region
    Article Snippet: Thus, by 2015, when explosive ZIKV outbreaks commenced in Brazil and later intensified throughout the Americas [ ], PHV was equipped to monitor and provide laboratory assessment of viraemic travellers suspected of ZIKV infection. .. ESR has provided DENV serological testing (Panbio, Alere, Australia) since 2008 and introduced a CHIKV immunofluorescence assay (IFA) (Euroimmun, Lübeck, Germany) and a ZIKV enzyme-linked immunosorbent assay (ELISA) (Euroimmun, Lübeck, Germany) in 2015 and 2016, respectively. .. Molecular RT-rtPCR diagnostic platforms were introduced for DENV 1–4 (2015), CHIKV (2015), and ZIKV (2014) in response to increased co-circulation of these viruses in the Pacific region combined with the escalating number of imported cases.

    Diagnostic Assay:

    Article Title: Antibody-based assay discriminates Zika virus infection from other flaviviruses
    Article Snippet: .. The diagnostic assessment for DENV, ZIKV, CHIKV, WNV, and YFV included the following: detection of anti-DENV IgM and IgG antibodies in serum samples (using dengue virus IgM Capture DxSelect and dengue virus IgG DxSelect, Focus Diagnostics, United States), detection of ZIKV IgM and IgG antibodies [anti-Zika virus ELISA (IgM) and anti-Zika virus ELISA (IgG); Euroimmun], identification of CHIKV IgM and IgG using specific immunofluorescence tests [anti-chikungunya virus IFA (IgG), anti-chikungunya virus IFA (IgM); Euroimmun], detection of WNV IgM and IgG using specific tests [NovaLisaTM Dengue IgM and IgG ELISAs (NovaTec Immunodiagnostic GmbH); and WNV IgM Capture DxSelect and WNV IgG DxSelect (Focus Diagnostics)], and detection of YFV IgM and IgG with anti-yellow fever virus IFA (IgG) and anti-yellow fever virus IFA (IgM; Euroimmun). .. Serology results were confirmed by specific viruses neutralization assays ( ).

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    EUROIMMUN igm igg indirect immunofluorescence assay ifa
    Proportions of positive chikungunya virus-specific RT-PCR, <t>IgM</t> and <t>IgG</t> antibodies (IFA) per day of sampling post symptom onset *. * Note: exact date of sampling was available for 261 patients with confirmed CHIKV infection.
    Igm Igg Indirect Immunofluorescence Assay Ifa, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    EUROIMMUN anti chikv igg
    Flowchart of recruitment of study participants and sample testing. Study participants were split into two groups, those who returned to the convalescent visit (left side of the flowchart) and those who were lost at follow-up (right side of the flow chart). The left side of the flow chart demonstrates that out of 400 recruited patients, 209 returned for the follow-up visit and their convalescent samples were initially screened for <t>anti-CHIKV</t> <t>IgG.</t> Out of 208 tested samples, 55 (26.4%) were positive and 153 (75.6%) were negative. Further, the corresponding 55 acute samples of the positive samples were screened for anti-CHIKV IgG. A total of 44 (21.2%) were positive with stationary titers, 3 (1.4%) were positive with four fold titer rise and 6 (2.9%) seroconverted. The right side of the flow chart demonstrates that 191 individuals did not return for their follow-up; 156 acute samples were available for testing and of these 38 (24.4%) and 118 (75.6%) were positive and negative, respectively. * Percentage against the total number of patients who returned to convalescent visit. ** Samples not tested because of insufficient volume
    Anti Chikv Igg, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti chikv igg/product/EUROIMMUN
    Average 86 stars, based on 1 article reviews
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    EUROIMMUN chikungunya virus
    Longitudinal variation of the sensitivity of IgM assays in the first seven days after fever onset. 1a: overall sensitivity based on samples collected from first and second <t>chikungunya</t> fever outbreaks in January and May to September 2008, respectively; 1b: sensitivity based on samples collected from the first outbreak due to CHIKV-A226 strain; 1c: sensitivity based on samples collected from the second outbreak due to CHIKV-226V strain. The numbers in brackets along the x-axis represent the number of samples tested for each day after onset of fever.
    Chikungunya Virus, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chikungunya virus/product/EUROIMMUN
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    EUROIMMUN assay anti chikv igg indirect immunofluorescence test iift
    Cross-reactivity of anti-alphaviruses and anti-flaviviruses antibodies with <t>CHIKV</t> E2EP3. Patient serum samples caused by non-CHIKV alphaviruses infections (n = 19) and flaviviruses infections (n = 60) were screened for cross-reactivity against CHIKV E2EP3 by peptide-based ELISA at a dilution of 1∶4000. A, Pie-chart shows the percentage of non-CHIKV alphaviruses-infected patients samples with positive or negative anti-E2EP3 antibody response. B, Pie-chart shows the percentage of flaviviruses-infected patient samples with positive or negative anti-E2EP3 antibody response. C - D, Pie-chart shows the percentage of DENV-infected and non-DENV flaviviruses-infected patient samples with positive or negative anti-E2EP3 <t>IgG</t> antibody response respectively. E, Detection of CHIKV E2EP3 by serum samples of unknown infections. Serum samples (n = 71) of febrile patients from unknown cause of infections were screened by peptide-based ELISA at a dilution of 1∶4000. Pie-chart shows the percentage of patients' samples with positive or negative anti-E2EP3 antibody response. F, Bar-chart shows the number of non-CHIKV alphaviruses-infected, E2EP3 cross-reactive samples with and without CHIKV neutralizing activity against the two CHIKV isolates (CHIKV (A226) and CHIKV (A226V)).
    Assay Anti Chikv Igg Indirect Immunofluorescence Test Iift, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Proportions of positive chikungunya virus-specific RT-PCR, IgM and IgG antibodies (IFA) per day of sampling post symptom onset *. * Note: exact date of sampling was available for 261 patients with confirmed CHIKV infection.

    Journal: PLoS ONE

    Article Title: Chikungunya virus infection in Aruba: Diagnosis, clinical features and predictors of post-chikungunya chronic polyarthralgia

    doi: 10.1371/journal.pone.0196630

    Figure Lengend Snippet: Proportions of positive chikungunya virus-specific RT-PCR, IgM and IgG antibodies (IFA) per day of sampling post symptom onset *. * Note: exact date of sampling was available for 261 patients with confirmed CHIKV infection.

    Article Snippet: Diagnostics Acute CHIKV infection in our study was defined by either or both positive results of CHIKV-specific IgM in an IgM/IgG indirect immunofluorescence assay (IFA) (Anti-CHIKV IIFT IgM/IgG, Euroimmun, Lübeck, Germany) according to manufacturer’s instructions, and CHIKV-specific real-time reverse-transcription polymerase chain reaction (RT-PCR).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Sampling, Infection

    Study design. * Acute phase sera refers to all available sera collected within 10 days of symptom onset. § Positive IgM: Immunoglobulin M (IgM) detected in indirect immunofluorescence assay (IFA) (Anti-CHIKV IIFT IgM/IgG, Euroimmun, Lübeck, Germany) according to manufacturer’s instructions. §§ positive RT-PCR: CHIKV RNA detected in a RT-PCR targeting the NSP-1 gene using primers and probes that detect CHIKV strain (LR2006_OPY_1, Indian Ocean Islands and the African prototype (S27, general strain)).

    Journal: PLoS ONE

    Article Title: Chikungunya virus infection in Aruba: Diagnosis, clinical features and predictors of post-chikungunya chronic polyarthralgia

    doi: 10.1371/journal.pone.0196630

    Figure Lengend Snippet: Study design. * Acute phase sera refers to all available sera collected within 10 days of symptom onset. § Positive IgM: Immunoglobulin M (IgM) detected in indirect immunofluorescence assay (IFA) (Anti-CHIKV IIFT IgM/IgG, Euroimmun, Lübeck, Germany) according to manufacturer’s instructions. §§ positive RT-PCR: CHIKV RNA detected in a RT-PCR targeting the NSP-1 gene using primers and probes that detect CHIKV strain (LR2006_OPY_1, Indian Ocean Islands and the African prototype (S27, general strain)).

    Article Snippet: Diagnostics Acute CHIKV infection in our study was defined by either or both positive results of CHIKV-specific IgM in an IgM/IgG indirect immunofluorescence assay (IFA) (Anti-CHIKV IIFT IgM/IgG, Euroimmun, Lübeck, Germany) according to manufacturer’s instructions, and CHIKV-specific real-time reverse-transcription polymerase chain reaction (RT-PCR).

    Techniques: Immunofluorescence, Reverse Transcription Polymerase Chain Reaction

    Flowchart of recruitment of study participants and sample testing. Study participants were split into two groups, those who returned to the convalescent visit (left side of the flowchart) and those who were lost at follow-up (right side of the flow chart). The left side of the flow chart demonstrates that out of 400 recruited patients, 209 returned for the follow-up visit and their convalescent samples were initially screened for anti-CHIKV IgG. Out of 208 tested samples, 55 (26.4%) were positive and 153 (75.6%) were negative. Further, the corresponding 55 acute samples of the positive samples were screened for anti-CHIKV IgG. A total of 44 (21.2%) were positive with stationary titers, 3 (1.4%) were positive with four fold titer rise and 6 (2.9%) seroconverted. The right side of the flow chart demonstrates that 191 individuals did not return for their follow-up; 156 acute samples were available for testing and of these 38 (24.4%) and 118 (75.6%) were positive and negative, respectively. * Percentage against the total number of patients who returned to convalescent visit. ** Samples not tested because of insufficient volume

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Serological Evidence of Chikungunya Virus among Acute Febrile Patients in Southern Mozambique

    doi: 10.1371/journal.pntd.0004146

    Figure Lengend Snippet: Flowchart of recruitment of study participants and sample testing. Study participants were split into two groups, those who returned to the convalescent visit (left side of the flowchart) and those who were lost at follow-up (right side of the flow chart). The left side of the flow chart demonstrates that out of 400 recruited patients, 209 returned for the follow-up visit and their convalescent samples were initially screened for anti-CHIKV IgG. Out of 208 tested samples, 55 (26.4%) were positive and 153 (75.6%) were negative. Further, the corresponding 55 acute samples of the positive samples were screened for anti-CHIKV IgG. A total of 44 (21.2%) were positive with stationary titers, 3 (1.4%) were positive with four fold titer rise and 6 (2.9%) seroconverted. The right side of the flow chart demonstrates that 191 individuals did not return for their follow-up; 156 acute samples were available for testing and of these 38 (24.4%) and 118 (75.6%) were positive and negative, respectively. * Percentage against the total number of patients who returned to convalescent visit. ** Samples not tested because of insufficient volume

    Article Snippet: Chikungunya virus immunofluorescence Convalescent serum samples were initially screened for anti-CHIKV IgG at a 1:20 dilution using a commercial indirect immunofluorescence test (IIFT) (EUROIMMUN AG, Lübeck, Germany).

    Techniques: Flow Cytometry

    Longitudinal variation of the sensitivity of IgM assays in the first seven days after fever onset. 1a: overall sensitivity based on samples collected from first and second chikungunya fever outbreaks in January and May to September 2008, respectively; 1b: sensitivity based on samples collected from the first outbreak due to CHIKV-A226 strain; 1c: sensitivity based on samples collected from the second outbreak due to CHIKV-226V strain. The numbers in brackets along the x-axis represent the number of samples tested for each day after onset of fever.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Evaluation of Chikungunya Diagnostic Assays: Differences in Sensitivity of Serology Assays in Two Independent Outbreaks

    doi: 10.1371/journal.pntd.0000753

    Figure Lengend Snippet: Longitudinal variation of the sensitivity of IgM assays in the first seven days after fever onset. 1a: overall sensitivity based on samples collected from first and second chikungunya fever outbreaks in January and May to September 2008, respectively; 1b: sensitivity based on samples collected from the first outbreak due to CHIKV-A226 strain; 1c: sensitivity based on samples collected from the second outbreak due to CHIKV-226V strain. The numbers in brackets along the x-axis represent the number of samples tested for each day after onset of fever.

    Article Snippet: Using sera collected in outbreaks caused by two variants of Chikungunya virus (A226 and 226V), we tested 2 commercial IgM tests (CTK lateral flow rapid test and EUROIMMUN IFA) alongside our in-house IgM assays (using both variants of the virus).

    Techniques:

    Cross-reactivity of anti-alphaviruses and anti-flaviviruses antibodies with CHIKV E2EP3. Patient serum samples caused by non-CHIKV alphaviruses infections (n = 19) and flaviviruses infections (n = 60) were screened for cross-reactivity against CHIKV E2EP3 by peptide-based ELISA at a dilution of 1∶4000. A, Pie-chart shows the percentage of non-CHIKV alphaviruses-infected patients samples with positive or negative anti-E2EP3 antibody response. B, Pie-chart shows the percentage of flaviviruses-infected patient samples with positive or negative anti-E2EP3 antibody response. C - D, Pie-chart shows the percentage of DENV-infected and non-DENV flaviviruses-infected patient samples with positive or negative anti-E2EP3 IgG antibody response respectively. E, Detection of CHIKV E2EP3 by serum samples of unknown infections. Serum samples (n = 71) of febrile patients from unknown cause of infections were screened by peptide-based ELISA at a dilution of 1∶4000. Pie-chart shows the percentage of patients' samples with positive or negative anti-E2EP3 antibody response. F, Bar-chart shows the number of non-CHIKV alphaviruses-infected, E2EP3 cross-reactive samples with and without CHIKV neutralizing activity against the two CHIKV isolates (CHIKV (A226) and CHIKV (A226V)).

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Sero-Prevalence and Cross-Reactivity of Chikungunya Virus Specific Anti-E2EP3 Antibodies in Arbovirus-Infected Patients

    doi: 10.1371/journal.pntd.0003445

    Figure Lengend Snippet: Cross-reactivity of anti-alphaviruses and anti-flaviviruses antibodies with CHIKV E2EP3. Patient serum samples caused by non-CHIKV alphaviruses infections (n = 19) and flaviviruses infections (n = 60) were screened for cross-reactivity against CHIKV E2EP3 by peptide-based ELISA at a dilution of 1∶4000. A, Pie-chart shows the percentage of non-CHIKV alphaviruses-infected patients samples with positive or negative anti-E2EP3 antibody response. B, Pie-chart shows the percentage of flaviviruses-infected patient samples with positive or negative anti-E2EP3 antibody response. C - D, Pie-chart shows the percentage of DENV-infected and non-DENV flaviviruses-infected patient samples with positive or negative anti-E2EP3 IgG antibody response respectively. E, Detection of CHIKV E2EP3 by serum samples of unknown infections. Serum samples (n = 71) of febrile patients from unknown cause of infections were screened by peptide-based ELISA at a dilution of 1∶4000. Pie-chart shows the percentage of patients' samples with positive or negative anti-E2EP3 antibody response. F, Bar-chart shows the number of non-CHIKV alphaviruses-infected, E2EP3 cross-reactive samples with and without CHIKV neutralizing activity against the two CHIKV isolates (CHIKV (A226) and CHIKV (A226V)).

    Article Snippet: Commercial assay Anti-CHIKV IgG indirect immunofluorescence test (IIFT) from EUROIMMUN AG (Lübeck, Germany) was used according to manufacturers' instructions.

    Techniques: Peptide ELISA, Infection, Activity Assay

    Association of E2EP3 cross-reactive antibody responses with CHIKV neutralizing activity. A, Anti-E2EP3 antibody response in serum samples (High E2EP3 IgG responders, n = 10; Low E2EP3 IgG responders, n = 15), at a dilution of 1∶4,000 were determined by ELISA using biotinylated E2EP3 peptides. Data are presented as mean ± SEM. Data are representative of two independent experiments with similar results. Statistical significance was measured using Mann-Whitney U test (*** P

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Sero-Prevalence and Cross-Reactivity of Chikungunya Virus Specific Anti-E2EP3 Antibodies in Arbovirus-Infected Patients

    doi: 10.1371/journal.pntd.0003445

    Figure Lengend Snippet: Association of E2EP3 cross-reactive antibody responses with CHIKV neutralizing activity. A, Anti-E2EP3 antibody response in serum samples (High E2EP3 IgG responders, n = 10; Low E2EP3 IgG responders, n = 15), at a dilution of 1∶4,000 were determined by ELISA using biotinylated E2EP3 peptides. Data are presented as mean ± SEM. Data are representative of two independent experiments with similar results. Statistical significance was measured using Mann-Whitney U test (*** P

    Article Snippet: Commercial assay Anti-CHIKV IgG indirect immunofluorescence test (IIFT) from EUROIMMUN AG (Lübeck, Germany) was used according to manufacturers' instructions.

    Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Antibody profiles of CHIKV-infected patients. A, Schematic diagram on the classification of clinical specimens. Four hundred and ten clinical specimens were included in this study, 339 samples have validated by in-house screening methods including 60 flaviviruses-positive samples and 279 alphaviruses-positive samples. Validated samples were further sub-categorized into 4 groups: CHIKV; Non-CHIKV (positive for alphavirus); DENV; and non-DENV (flavivirus-positive). Another 71 samples of febrile patients with unknown cause of infection were also included in this study. B, CHIKV E2EP3-specific IgG antibody detection in patients' serum samples (total 38 patients, n = 260) at a dilution of 1∶4000 were determined by peptide-based ELISA. Black solid line represents the mean value of the healthy donors and dotted line represents the value of mean ±3 SD. Values above mean ±3 SD are considered positive anti-E2EP3 IgG antibody response. Data are presented as mean. Patient samples were categorized into different phase including acute (1–6 days); early convalescent (7–13 days); late convalescent (14–29 days); chronic (30–176 days). C , Pie-chart shows the percentage of patients with positive or negative anti-E2EP3 IgG antibody response (upper panel), and the percentage of patients with positive or negative anti-CHIKV IgG response using immunofluorescence-based biochips (CHIKV IIFT, EUROIMMUN AG) (lower panel).

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Sero-Prevalence and Cross-Reactivity of Chikungunya Virus Specific Anti-E2EP3 Antibodies in Arbovirus-Infected Patients

    doi: 10.1371/journal.pntd.0003445

    Figure Lengend Snippet: Antibody profiles of CHIKV-infected patients. A, Schematic diagram on the classification of clinical specimens. Four hundred and ten clinical specimens were included in this study, 339 samples have validated by in-house screening methods including 60 flaviviruses-positive samples and 279 alphaviruses-positive samples. Validated samples were further sub-categorized into 4 groups: CHIKV; Non-CHIKV (positive for alphavirus); DENV; and non-DENV (flavivirus-positive). Another 71 samples of febrile patients with unknown cause of infection were also included in this study. B, CHIKV E2EP3-specific IgG antibody detection in patients' serum samples (total 38 patients, n = 260) at a dilution of 1∶4000 were determined by peptide-based ELISA. Black solid line represents the mean value of the healthy donors and dotted line represents the value of mean ±3 SD. Values above mean ±3 SD are considered positive anti-E2EP3 IgG antibody response. Data are presented as mean. Patient samples were categorized into different phase including acute (1–6 days); early convalescent (7–13 days); late convalescent (14–29 days); chronic (30–176 days). C , Pie-chart shows the percentage of patients with positive or negative anti-E2EP3 IgG antibody response (upper panel), and the percentage of patients with positive or negative anti-CHIKV IgG response using immunofluorescence-based biochips (CHIKV IIFT, EUROIMMUN AG) (lower panel).

    Article Snippet: Commercial assay Anti-CHIKV IgG indirect immunofluorescence test (IIFT) from EUROIMMUN AG (Lübeck, Germany) was used according to manufacturers' instructions.

    Techniques: Infection, Peptide ELISA, Immunofluorescence