chemiluminescence kit  (Millipore)


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    Structured Review

    Millipore chemiluminescence kit
    Chemiluminescence Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 594 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemiluminescence kit/product/Millipore
    Average 99 stars, based on 594 article reviews
    Price from $9.99 to $1999.99
    chemiluminescence kit - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Western Blot:

    Article Title: WP1130 Enhances TRAIL-Induced Apoptosis through USP9X-Dependent miR-708-Mediated Downregulation of c-FLIP
    Article Snippet: .. After overnight incubation, the membranes were incubated with secondary antibodies for 2 h, and were exposed using an enhanced chemiluminescence Western blot kit (EMD Millipore, Darmstadt, Germany). ..

    Article Title: Maritoclax Enhances TRAIL-Induced Apoptosis via CHOP-Mediated Upregulation of DR5 and miR-708-Mediated Downregulation of cFLIP
    Article Snippet: .. The membranes were exposed using an enhanced chemiluminescence Western blot kit (EMD Millipore, Darmstadt, Germany). ..

    Article Title: Sublytic Membrane-Attack-Complex (MAC) Activation Alters Regulated Rather than Constitutive Vascular Endothelial Growth Factor (VEGF) Secretion in Retinal Pigment Epithelium Monolayers *
    Article Snippet: .. The membrane was probed with primary antibodies; antibody binding was visualized using a chemiluminescence detection kit (Immobilon Western; Millipore Corporation, Billerica, MA). .. The intensity of the bands was quantified using the Alpha Innotech Fluorchem 9900 imaging system running Alpha Ease FC Software 3.3 (Alpha Innotech, San Leandro, CA).

    Article Title: Downregulation of microRNA-425-5p suppresses cervical cancer tumorigenesis by targeting AIFM1
    Article Snippet: .. Protein bands were visualized using the enhanced chemiluminescence Western Blotting Detection kit (EMD Millipore). .. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) Total RNA was extracted from cells using TRIzol® reagent (Invitrogen, Thermo Fisher Scientific, Inc.), according to the manufacturer's protocol.

    Article Title: NT21MP negatively regulates paclitaxel-resistant cells by targeting miR-155-3p and miR-155-5p via the CXCR4 pathway in breast cancer
    Article Snippet: .. The immune complexes were detected using an Enhanced Chemiluminescence Western Blotting kit (EMD Millipore, Billerica, MA, USA) and Bio-Rad Image-Lab software 5.2.1 (Bio-Rad Laboratories, Inc.). .. The relative protein expression was determined using ImageJ V1.8.0 (National Institutes of Health, Bethesda, MD, USA), with GAPDH used as the internal reference.

    Article Title: Cell specific apoptosis by RLX is mediated by NFκB in human colon carcinoma HCT-116 cells
    Article Snippet: .. A dilution of secondary antibody (mouse and rabbit) conjugate was added for 1 h of incubation and signals were detected using Millipore Chemiluminescent western blotting kit and analyzed using X-ray film [ ]. .. Cell migration Assay HCT-116 cell monolayer (90% confluent) was allowed to become quiescent in medium with 0.1% dialyzed fetal bovine serum for 24 h. Further, cells were scraped to make a straight line wound and treated with RLX and BEZ-235 (Positive control) for 48 h. Photographs were taken through an inverted microscope (×40 magnification) at 48 h and lengths of wound were determined by Image J (version 1.46) software [ ].

    Incubation:

    Article Title: WP1130 Enhances TRAIL-Induced Apoptosis through USP9X-Dependent miR-708-Mediated Downregulation of c-FLIP
    Article Snippet: .. After overnight incubation, the membranes were incubated with secondary antibodies for 2 h, and were exposed using an enhanced chemiluminescence Western blot kit (EMD Millipore, Darmstadt, Germany). ..

    Article Title: Cell specific apoptosis by RLX is mediated by NFκB in human colon carcinoma HCT-116 cells
    Article Snippet: .. A dilution of secondary antibody (mouse and rabbit) conjugate was added for 1 h of incubation and signals were detected using Millipore Chemiluminescent western blotting kit and analyzed using X-ray film [ ]. .. Cell migration Assay HCT-116 cell monolayer (90% confluent) was allowed to become quiescent in medium with 0.1% dialyzed fetal bovine serum for 24 h. Further, cells were scraped to make a straight line wound and treated with RLX and BEZ-235 (Positive control) for 48 h. Photographs were taken through an inverted microscope (×40 magnification) at 48 h and lengths of wound were determined by Image J (version 1.46) software [ ].

    Article Title: Characterization of DNA hydroxymethylation in the hypothalamus of elderly mice with post-operative cognitive dysfunction
    Article Snippet: .. Membranes were washed with TBST and incubated with goat anti-mouse and goat anti-rabbit IgG-HRP (1:10,000; 20 ng/ml, Beyotime Institute of Biotechnology) at room temperature for 1 h. Membranes were then treated with aBM Chemiluminescence Western Blotting Kit (cat. no. 11520709001; EMD Millipore), and the bands were captured to evaluate the difference of protein expression. .. Immunofluorescence assay In brief, mouse tissue was fixed using 4% paraformaldehyde at room temperature for 24 h then dehydrated with 50–90% ethanol.

    Binding Assay:

    Article Title: Sublytic Membrane-Attack-Complex (MAC) Activation Alters Regulated Rather than Constitutive Vascular Endothelial Growth Factor (VEGF) Secretion in Retinal Pigment Epithelium Monolayers *
    Article Snippet: .. The membrane was probed with primary antibodies; antibody binding was visualized using a chemiluminescence detection kit (Immobilon Western; Millipore Corporation, Billerica, MA). .. The intensity of the bands was quantified using the Alpha Innotech Fluorchem 9900 imaging system running Alpha Ease FC Software 3.3 (Alpha Innotech, San Leandro, CA).

    Expressing:

    Article Title: Characterization of DNA hydroxymethylation in the hypothalamus of elderly mice with post-operative cognitive dysfunction
    Article Snippet: .. Membranes were washed with TBST and incubated with goat anti-mouse and goat anti-rabbit IgG-HRP (1:10,000; 20 ng/ml, Beyotime Institute of Biotechnology) at room temperature for 1 h. Membranes were then treated with aBM Chemiluminescence Western Blotting Kit (cat. no. 11520709001; EMD Millipore), and the bands were captured to evaluate the difference of protein expression. .. Immunofluorescence assay In brief, mouse tissue was fixed using 4% paraformaldehyde at room temperature for 24 h then dehydrated with 50–90% ethanol.

    Software:

    Article Title: NT21MP negatively regulates paclitaxel-resistant cells by targeting miR-155-3p and miR-155-5p via the CXCR4 pathway in breast cancer
    Article Snippet: .. The immune complexes were detected using an Enhanced Chemiluminescence Western Blotting kit (EMD Millipore, Billerica, MA, USA) and Bio-Rad Image-Lab software 5.2.1 (Bio-Rad Laboratories, Inc.). .. The relative protein expression was determined using ImageJ V1.8.0 (National Institutes of Health, Bethesda, MD, USA), with GAPDH used as the internal reference.

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    Millipore braf kinase assay kit
    Autoantibodies to <t>BRAF</t> activate the phosphorylation of <t>MEK1</t> . Purified anti-BRAF autoantibodies from patients were incubated with BRAF and MEK1. For each patient, we included one positive control (BRAF and MEK1) and one negative control (BRAF and MEK1 and C1 control antibody). Activation or inhibition of MEK1 phosphorylation was detected by measuring the ratio test OD/positive control OD. Ratio > 1 indicated activation, ratio
    Braf Kinase Assay Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/braf kinase assay kit/product/Millipore
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    braf kinase assay kit - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Millipore chemiluminescence immobilon western kit
    ). Immunoreactive bands were detected in solution containing dissolved SigmaFast 3,3-diaminobenzidine tablets (GOX) or detected using an enhanced chemiluminescence <t>Immobilon</t> Western kit (Def-1).
    Chemiluminescence Immobilon Western Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemiluminescence immobilon western kit/product/Millipore
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    chemiluminescence immobilon western kit - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    99
    Millipore chemiluminescence immunoblotting kit
    RACK1 associates with LC3. A , HEK293T cells were starved in HBSS before being harvested for anti-LC3 immunoprecipitation followed by <t>immunoblotting.</t> BFA1 was added to HBSS to block LC3 degradation in starvation-induced autophagy. The total cell lysates
    Chemiluminescence Immunoblotting Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemiluminescence immunoblotting kit/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chemiluminescence immunoblotting kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Autoantibodies to BRAF activate the phosphorylation of MEK1 . Purified anti-BRAF autoantibodies from patients were incubated with BRAF and MEK1. For each patient, we included one positive control (BRAF and MEK1) and one negative control (BRAF and MEK1 and C1 control antibody). Activation or inhibition of MEK1 phosphorylation was detected by measuring the ratio test OD/positive control OD. Ratio > 1 indicated activation, ratio

    Journal: Arthritis Research & Therapy

    Article Title: Autoantibodies to BRAF, a new family of autoantibodies associated with rheumatoid arthritis

    doi: 10.1186/ar3165

    Figure Lengend Snippet: Autoantibodies to BRAF activate the phosphorylation of MEK1 . Purified anti-BRAF autoantibodies from patients were incubated with BRAF and MEK1. For each patient, we included one positive control (BRAF and MEK1) and one negative control (BRAF and MEK1 and C1 control antibody). Activation or inhibition of MEK1 phosphorylation was detected by measuring the ratio test OD/positive control OD. Ratio > 1 indicated activation, ratio

    Article Snippet: BRAF kinase assay BRAF dependent phosphotransferase activity was measured in a kinase reaction using inactive recombinant MEK1 as a BRAF substrate (BRAF kinase assay Kit, chemiluminescence detection, Millipore Upstate Temecula, CA, USA).

    Techniques: Purification, Incubation, Positive Control, Negative Control, Activation Assay, Inhibition

    ). Immunoreactive bands were detected in solution containing dissolved SigmaFast 3,3-diaminobenzidine tablets (GOX) or detected using an enhanced chemiluminescence Immobilon Western kit (Def-1).

    Journal: Scientific Reports

    Article Title: Phytochemicals-mediated production of hydrogen peroxide is crucial for high antibacterial activity of honeydew honey

    doi: 10.1038/s41598-018-27449-3

    Figure Lengend Snippet: ). Immunoreactive bands were detected in solution containing dissolved SigmaFast 3,3-diaminobenzidine tablets (GOX) or detected using an enhanced chemiluminescence Immobilon Western kit (Def-1).

    Article Snippet: Immunoreactive bands were detected in solution containing dissolved SigmaFast 3,3-diaminobenzidine tablets (Sigma-Aldrich) or detected using enhanced chemiluminescence Immobilon Western kit (Millipore, MA, USA).

    Techniques: Western Blot

    Detection of glucose oxidase (GOX) and defensin-1 (Def-1) in honeydew honey samples (n = 23) and medical-grade manuka and kanuka honey following proteinase K treatment by immunoblotting. Aliquots (15 μl) of 50% (w/v) honey solution with or without proteinase K were resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and 16.5% Tricine-SDS-PAGE. After semi-dry blotting procedure, the blocked membrane was incubated overnight with a rabbit polyclonal antibody against honeybee GOX or Def-1 (1:2000). Shown are cropped blots. (The blots with indicated cropping lines are shown in Supplementary Fig. S2 ). Immunoreactive bands were detected in solution containing dissolved SigmaFast 3,3-diaminobenzidine tablets (GOX) or detected using an enhanced chemiluminescence Immobilon Western kit (Def-1).

    Journal: Scientific Reports

    Article Title: Phytochemicals-mediated production of hydrogen peroxide is crucial for high antibacterial activity of honeydew honey

    doi: 10.1038/s41598-018-27449-3

    Figure Lengend Snippet: Detection of glucose oxidase (GOX) and defensin-1 (Def-1) in honeydew honey samples (n = 23) and medical-grade manuka and kanuka honey following proteinase K treatment by immunoblotting. Aliquots (15 μl) of 50% (w/v) honey solution with or without proteinase K were resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and 16.5% Tricine-SDS-PAGE. After semi-dry blotting procedure, the blocked membrane was incubated overnight with a rabbit polyclonal antibody against honeybee GOX or Def-1 (1:2000). Shown are cropped blots. (The blots with indicated cropping lines are shown in Supplementary Fig. S2 ). Immunoreactive bands were detected in solution containing dissolved SigmaFast 3,3-diaminobenzidine tablets (GOX) or detected using an enhanced chemiluminescence Immobilon Western kit (Def-1).

    Article Snippet: Immunoreactive bands were detected in solution containing dissolved SigmaFast 3,3-diaminobenzidine tablets (Sigma-Aldrich) or detected using enhanced chemiluminescence Immobilon Western kit (Millipore, MA, USA).

    Techniques: Polyacrylamide Gel Electrophoresis, SDS Page, Semi Dry Blot, Incubation, Western Blot

    RACK1 associates with LC3. A , HEK293T cells were starved in HBSS before being harvested for anti-LC3 immunoprecipitation followed by immunoblotting. BFA1 was added to HBSS to block LC3 degradation in starvation-induced autophagy. The total cell lysates

    Journal: The Journal of Biological Chemistry

    Article Title: Receptor for Activated C Kinase 1 (RACK1) Promotes Dishevelled Protein Degradation via Autophagy and Antagonizes Wnt Signaling *

    doi: 10.1074/jbc.M115.708818

    Figure Lengend Snippet: RACK1 associates with LC3. A , HEK293T cells were starved in HBSS before being harvested for anti-LC3 immunoprecipitation followed by immunoblotting. BFA1 was added to HBSS to block LC3 degradation in starvation-induced autophagy. The total cell lysates

    Article Snippet: The membranes were incubated with primary antibodies at 4 °C overnight and then incubated with secondary antibodies at room temperature for 1 h. The specific proteins were detected using a chemiluminescence immunoblotting kit (Millipore).

    Techniques: Immunoprecipitation, Blocking Assay

    RACK1 promotes Dvl degradation. A–C , HEK293T cells were transfected with FLAG-Dvl1, Dvl2, or Dvl3 and Myc-RACK1 after 24 h and then harvested for immunoblotting. Myc-RACK1 was transfected with concentration gradients of 0.25, 0.5, and 1.0 μg

    Journal: The Journal of Biological Chemistry

    Article Title: Receptor for Activated C Kinase 1 (RACK1) Promotes Dishevelled Protein Degradation via Autophagy and Antagonizes Wnt Signaling *

    doi: 10.1074/jbc.M115.708818

    Figure Lengend Snippet: RACK1 promotes Dvl degradation. A–C , HEK293T cells were transfected with FLAG-Dvl1, Dvl2, or Dvl3 and Myc-RACK1 after 24 h and then harvested for immunoblotting. Myc-RACK1 was transfected with concentration gradients of 0.25, 0.5, and 1.0 μg

    Article Snippet: The membranes were incubated with primary antibodies at 4 °C overnight and then incubated with secondary antibodies at room temperature for 1 h. The specific proteins were detected using a chemiluminescence immunoblotting kit (Millipore).

    Techniques: Transfection, Concentration Assay