chemicals liquid chromatography mass spectrometric  (Millipore)


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    Name:
    Liquid Chromatography
    Description:
    With the advent of new interfacing technology the benefits of LC MS liquid chromatography with mass spectrometry are being realized by the growth in applications of this technique in both the chemical and life sciences Topics covered include Limitations of the component techniques when used in isolation and how a combination of the two allows these to be overcome Descriptions of the various approaches including thermospray electrospray and atmospheric pressure chemical ionization which are used to interface the two techniques along with the advantages and disadvantages of each system Illustrative examples of the applications of LC MS including the molecular weight and structure determinations of both biopolymers and low molecular compounds
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    z702080
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    Millipore chemicals liquid chromatography mass spectrometric
    Liquid Chromatography
    With the advent of new interfacing technology the benefits of LC MS liquid chromatography with mass spectrometry are being realized by the growth in applications of this technique in both the chemical and life sciences Topics covered include Limitations of the component techniques when used in isolation and how a combination of the two allows these to be overcome Descriptions of the various approaches including thermospray electrospray and atmospheric pressure chemical ionization which are used to interface the two techniques along with the advantages and disadvantages of each system Illustrative examples of the applications of LC MS including the molecular weight and structure determinations of both biopolymers and low molecular compounds
    https://www.bioz.com/result/chemicals liquid chromatography mass spectrometric/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chemicals liquid chromatography mass spectrometric - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Morusinol Exhibits Selective and Potent Antitumor Activity Against Human Liver Carcinoma by Inducing Autophagy, G2/M Cell Cycle Arrest, Inhibition of Cell Invasion and Migration, and Targeting of Ras/MEK/ERK Pathway
    Article Snippet: .. Chemicals and other reagents Morusinol (purity > 98%; determined by high-performance liquid chromatography), 3-(4, 5-dimethyl-2-thiazolyl)-2, and 5-diphenyl-2H-tetrazolium bromide (MTT) were obtained from SigmaAldrich Chemical Co. (St. Louis, MO, USA). .. Propidium iodide was purchased from Wuhan Boster Biological Technology (Wuhan, China).

    Article Title: Remodeling of Retinal Fatty Acids in an Animal Model of Diabetes
    Article Snippet: .. High-performance liquid chromatography (HPLC)-grade acetonitrile, acetic acid, methanol, chloroform, streptozotocin (STZ), and commonly used chemicals and reagents were from Sigma-Aldrich Chemical (St. Louis, MO). ..

    MTT Assay:

    Article Title: Morusinol Exhibits Selective and Potent Antitumor Activity Against Human Liver Carcinoma by Inducing Autophagy, G2/M Cell Cycle Arrest, Inhibition of Cell Invasion and Migration, and Targeting of Ras/MEK/ERK Pathway
    Article Snippet: .. Chemicals and other reagents Morusinol (purity > 98%; determined by high-performance liquid chromatography), 3-(4, 5-dimethyl-2-thiazolyl)-2, and 5-diphenyl-2H-tetrazolium bromide (MTT) were obtained from SigmaAldrich Chemical Co. (St. Louis, MO, USA). .. Propidium iodide was purchased from Wuhan Boster Biological Technology (Wuhan, China).

    Mass Spectrometry:

    Article Title: Enhanced dissolution and oral absorption of tacrolimus by supersaturable self-emulsifying drug delivery system
    Article Snippet: .. Ascomycin (purity > 98% w/w%) used as an internal standard for FK506 Liquid Chromatography-Tandem Mass Spectrometry (LC–MS/MS) analysis was obtained from Sigma-Aldrich Co. (St Louis, MO, USA). .. Cremophor EL and Soluplus were kindly provided by BASF (Ludwigshafen, Germany).

    Chromatography:

    Article Title: Enhanced dissolution and oral absorption of tacrolimus by supersaturable self-emulsifying drug delivery system
    Article Snippet: .. Ascomycin (purity > 98% w/w%) used as an internal standard for FK506 Liquid Chromatography-Tandem Mass Spectrometry (LC–MS/MS) analysis was obtained from Sigma-Aldrich Co. (St Louis, MO, USA). .. Cremophor EL and Soluplus were kindly provided by BASF (Ludwigshafen, Germany).

    Article Title: Formation of m2G6 in Methanocaldococcus jannaschii tRNA catalyzed by the novel methyltransferase Trm14
    Article Snippet: .. For liquid chromatography–mass spectrometry (LC–MS) analysis of modified nucleosides, nuclease P1 (Sigma), snake venom phosphodiesterase I (Worthington Biochemical Corporation) and Antarctic phosphatase (New England Biolabs) were used to digest Trm14-reacted transcripts and control unmethylated transcripts to nucleosides. .. The nucleosides were separated using a Hitachi D-7000 HPLC with a Hitachi L-7400 UV detector at 0.3 ml/min at room temperature on an LC-18 S 2.1 × 250 mm column from Supelco using a gradient of 5 mM ammonium acetate pH 5.3 and acetonitrile:water (40:60 v/v) as described ( ).

    Article Title: Unexpected Different Binding of Mistletoe Lectins from Plant Extracts to Immobilized Lactose and N-acetylgalactosamine
    Article Snippet: .. Fast protein liquid chromatography (FPLC) separation of eluted MLs using cation exchange chromatography The eluate from the affigel-asialofetuin column was dialysed against 15 mM citrate buffer (pH 4.2), then concentrated to 2 mg/ml using Microcon YM-30 tubes (Millipore, Switzerland) before applying to a cation exchange chromatography column (Mono S 5/50 GL; Amersham Bioscience, Switzerland). .. The column was adapted to a FPLC system and equilibrated with citrate buffer (pH 4.2).

    Isolation:

    Article Title: Attenuation of atherogenic apo B-48-dependent hyperlipidemia and high density lipoprotein remodeling induced by vitamin C and E combination and their beneficial effect on lethal ischemic heart disease in mice
    Article Snippet: .. Lipoprotein fractionation and high density lipoprotein isolation Serum was fractionated by fast-protein liquid chromatography (FPLC) and apolipoprotein A-I-containing fractions were pooled and concentrated using Amicon Ultra-4 centrifugal 10 K filters (Millipore, Merck, Darmstadt, HD, Germany). .. Pooled fractions were subjected to dynabead-based immunoprecipitation with an anti-apolipoprotein B (apo B) antibody to remove apo B-containing lipoproteins.

    Fractionation:

    Article Title: Attenuation of atherogenic apo B-48-dependent hyperlipidemia and high density lipoprotein remodeling induced by vitamin C and E combination and their beneficial effect on lethal ischemic heart disease in mice
    Article Snippet: .. Lipoprotein fractionation and high density lipoprotein isolation Serum was fractionated by fast-protein liquid chromatography (FPLC) and apolipoprotein A-I-containing fractions were pooled and concentrated using Amicon Ultra-4 centrifugal 10 K filters (Millipore, Merck, Darmstadt, HD, Germany). .. Pooled fractions were subjected to dynabead-based immunoprecipitation with an anti-apolipoprotein B (apo B) antibody to remove apo B-containing lipoproteins.

    Fast Protein Liquid Chromatography:

    Article Title: Attenuation of atherogenic apo B-48-dependent hyperlipidemia and high density lipoprotein remodeling induced by vitamin C and E combination and their beneficial effect on lethal ischemic heart disease in mice
    Article Snippet: .. Serum was fractionated by fast-protein liquid chromatography (FPLC) and apolipoprotein A-I-containing fractions were pooled and concentrated using Amicon Ultra-4 centrifugal 10 K filters (Millipore, Merck, Darmstadt, HD, Germany). .. Pooled fractions were subjected to dynabead-based immunoprecipitation with an anti-apolipoprotein B (apo B) antibody to remove apo B-containing lipoproteins.

    Article Title: Attenuation of atherogenic apo B-48-dependent hyperlipidemia and high density lipoprotein remodeling induced by vitamin C and E combination and their beneficial effect on lethal ischemic heart disease in mice
    Article Snippet: .. Lipoprotein fractionation and high density lipoprotein isolation Serum was fractionated by fast-protein liquid chromatography (FPLC) and apolipoprotein A-I-containing fractions were pooled and concentrated using Amicon Ultra-4 centrifugal 10 K filters (Millipore, Merck, Darmstadt, HD, Germany). .. Pooled fractions were subjected to dynabead-based immunoprecipitation with an anti-apolipoprotein B (apo B) antibody to remove apo B-containing lipoproteins.

    Article Title: Unexpected Different Binding of Mistletoe Lectins from Plant Extracts to Immobilized Lactose and N-acetylgalactosamine
    Article Snippet: .. Fast protein liquid chromatography (FPLC) separation of eluted MLs using cation exchange chromatography The eluate from the affigel-asialofetuin column was dialysed against 15 mM citrate buffer (pH 4.2), then concentrated to 2 mg/ml using Microcon YM-30 tubes (Millipore, Switzerland) before applying to a cation exchange chromatography column (Mono S 5/50 GL; Amersham Bioscience, Switzerland). .. The column was adapted to a FPLC system and equilibrated with citrate buffer (pH 4.2).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Formation of m2G6 in Methanocaldococcus jannaschii tRNA catalyzed by the novel methyltransferase Trm14
    Article Snippet: .. For liquid chromatography–mass spectrometry (LC–MS) analysis of modified nucleosides, nuclease P1 (Sigma), snake venom phosphodiesterase I (Worthington Biochemical Corporation) and Antarctic phosphatase (New England Biolabs) were used to digest Trm14-reacted transcripts and control unmethylated transcripts to nucleosides. .. The nucleosides were separated using a Hitachi D-7000 HPLC with a Hitachi L-7400 UV detector at 0.3 ml/min at room temperature on an LC-18 S 2.1 × 250 mm column from Supelco using a gradient of 5 mM ammonium acetate pH 5.3 and acetonitrile:water (40:60 v/v) as described ( ).

    Modification:

    Article Title: Formation of m2G6 in Methanocaldococcus jannaschii tRNA catalyzed by the novel methyltransferase Trm14
    Article Snippet: .. For liquid chromatography–mass spectrometry (LC–MS) analysis of modified nucleosides, nuclease P1 (Sigma), snake venom phosphodiesterase I (Worthington Biochemical Corporation) and Antarctic phosphatase (New England Biolabs) were used to digest Trm14-reacted transcripts and control unmethylated transcripts to nucleosides. .. The nucleosides were separated using a Hitachi D-7000 HPLC with a Hitachi L-7400 UV detector at 0.3 ml/min at room temperature on an LC-18 S 2.1 × 250 mm column from Supelco using a gradient of 5 mM ammonium acetate pH 5.3 and acetonitrile:water (40:60 v/v) as described ( ).

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  • 86
    Millipore hpiv3 infected hela cell lysates
    Immunoprecipitation and flow cytometry assay. (A) Recombinant <t>GST-HPIV3-HN</t> or GST protein was immnoprecipitated with either #7, #21, #23 MAbs, or IgG (negative control), respectively. Then bound proteins were analyzed with immunoblotting using anti-GST antibody. (B) HPIV3-infected or uninfected <t>HeLa</t> cells were harvested at 4 days post-infection, followed by incubation with indicated MAbs. The cells were then fixed and stained with anti-mouse secondary antibody. The population of stained cells was calculated by flow cytometry. The shaded histogram shows negative hybridoma supernatant and the bold line shows specific MAbs.
    Hpiv3 Infected Hela Cell Lysates, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpiv3 infected hela cell lysates/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hpiv3 infected hela cell lysates - by Bioz Stars, 2020-09
    86/100 stars
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    Immunoprecipitation and flow cytometry assay. (A) Recombinant GST-HPIV3-HN or GST protein was immnoprecipitated with either #7, #21, #23 MAbs, or IgG (negative control), respectively. Then bound proteins were analyzed with immunoblotting using anti-GST antibody. (B) HPIV3-infected or uninfected HeLa cells were harvested at 4 days post-infection, followed by incubation with indicated MAbs. The cells were then fixed and stained with anti-mouse secondary antibody. The population of stained cells was calculated by flow cytometry. The shaded histogram shows negative hybridoma supernatant and the bold line shows specific MAbs.

    Journal: Frontiers in Microbiology

    Article Title: Wheat germ cell-free system-based production of hemagglutinin-neuraminidase glycoprotein of human parainfluenza virus type 3 for generation and characterization of monoclonal antibody

    doi: 10.3389/fmicb.2014.00208

    Figure Lengend Snippet: Immunoprecipitation and flow cytometry assay. (A) Recombinant GST-HPIV3-HN or GST protein was immnoprecipitated with either #7, #21, #23 MAbs, or IgG (negative control), respectively. Then bound proteins were analyzed with immunoblotting using anti-GST antibody. (B) HPIV3-infected or uninfected HeLa cells were harvested at 4 days post-infection, followed by incubation with indicated MAbs. The cells were then fixed and stained with anti-mouse secondary antibody. The population of stained cells was calculated by flow cytometry. The shaded histogram shows negative hybridoma supernatant and the bold line shows specific MAbs.

    Article Snippet: IMMUNOBLOTTING Recombinant HPIV3-HN proteins (equivalent to ~100 ng) or HPIV3-infected HeLa cell lysates were separated by 10% SDS-Gel and transferred onto a PVDF membrane (Millipore).

    Techniques: Immunoprecipitation, Flow Cytometry, Cytometry, Recombinant, Negative Control, Infection, Incubation, Staining

    Immunoblotting and immunofluorescent analysis. (A,B) Detection sensitivity of the MAbs for recombinant His-HN (A) or HPIV3-infected cell lysate (B) . Recombinant HPIV3-HN (100 ng) was separated using 12.5% SDS-gel and transferred to a PVDF membrane, followed by incubation with MAbs (hybridoma supernatants) at a 1:10 dilution (A) . HeLa cells were infected or mock-infected with HPIV3. After 48 h, cells were lysed with SDS-PAGE loading buffer. The total protein was separated in 12.5% SDS-gel and immunobloted with indicated MAbs (B) . (C,D) Immunofluoresent analysis of HN (red) in HPIV3-infected HeLa cells. HeLa cells were infected or mock-infected with HPIV3. After 48 h, cells were fixed, and then stained with MAbs (hybridoma supernatant; red) and DAPI (blue). Confocal microscopic analysis was performed at 40× (C) and at 600× magnifications (D) .

    Journal: Frontiers in Microbiology

    Article Title: Wheat germ cell-free system-based production of hemagglutinin-neuraminidase glycoprotein of human parainfluenza virus type 3 for generation and characterization of monoclonal antibody

    doi: 10.3389/fmicb.2014.00208

    Figure Lengend Snippet: Immunoblotting and immunofluorescent analysis. (A,B) Detection sensitivity of the MAbs for recombinant His-HN (A) or HPIV3-infected cell lysate (B) . Recombinant HPIV3-HN (100 ng) was separated using 12.5% SDS-gel and transferred to a PVDF membrane, followed by incubation with MAbs (hybridoma supernatants) at a 1:10 dilution (A) . HeLa cells were infected or mock-infected with HPIV3. After 48 h, cells were lysed with SDS-PAGE loading buffer. The total protein was separated in 12.5% SDS-gel and immunobloted with indicated MAbs (B) . (C,D) Immunofluoresent analysis of HN (red) in HPIV3-infected HeLa cells. HeLa cells were infected or mock-infected with HPIV3. After 48 h, cells were fixed, and then stained with MAbs (hybridoma supernatant; red) and DAPI (blue). Confocal microscopic analysis was performed at 40× (C) and at 600× magnifications (D) .

    Article Snippet: IMMUNOBLOTTING Recombinant HPIV3-HN proteins (equivalent to ~100 ng) or HPIV3-infected HeLa cell lysates were separated by 10% SDS-Gel and transferred onto a PVDF membrane (Millipore).

    Techniques: Recombinant, Infection, SDS-Gel, Incubation, SDS Page, Staining

    Proteomics analysis using selected MAb. (A) Schematic diagram of proteomic analysis for the identification of PIV3-HN-binding protein. HPIV3-infected HeLa cell lysate was immunoprecipitated with #21 MAb. The bound proteins were separated by SDS-PAGE and analyzed by LC-Ms/Ms. (B) The panel shows the list of putative HN-binding proteins identified by mass spectrometry analysis. (C) FLAG-tagged HSP70, HSP90, tubulin alpha 1C, or SERPINA3 proteins were mixed with HPIV3-HN. Samples were pull-down with the streptavidin magnetic beads and the collected proteins were separated by SDS-PAGE. The bound protein detected by immunoblotting analysis with anti-FLAG antibody. The right arrows indicated the position of each protein.

    Journal: Frontiers in Microbiology

    Article Title: Wheat germ cell-free system-based production of hemagglutinin-neuraminidase glycoprotein of human parainfluenza virus type 3 for generation and characterization of monoclonal antibody

    doi: 10.3389/fmicb.2014.00208

    Figure Lengend Snippet: Proteomics analysis using selected MAb. (A) Schematic diagram of proteomic analysis for the identification of PIV3-HN-binding protein. HPIV3-infected HeLa cell lysate was immunoprecipitated with #21 MAb. The bound proteins were separated by SDS-PAGE and analyzed by LC-Ms/Ms. (B) The panel shows the list of putative HN-binding proteins identified by mass spectrometry analysis. (C) FLAG-tagged HSP70, HSP90, tubulin alpha 1C, or SERPINA3 proteins were mixed with HPIV3-HN. Samples were pull-down with the streptavidin magnetic beads and the collected proteins were separated by SDS-PAGE. The bound protein detected by immunoblotting analysis with anti-FLAG antibody. The right arrows indicated the position of each protein.

    Article Snippet: IMMUNOBLOTTING Recombinant HPIV3-HN proteins (equivalent to ~100 ng) or HPIV3-infected HeLa cell lysates were separated by 10% SDS-Gel and transferred onto a PVDF membrane (Millipore).

    Techniques: Binding Assay, Infection, Immunoprecipitation, SDS Page, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Magnetic Beads