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Effects of methyl-β-cyclodextrin <t>(MβCD)</t> and cholesterol (chol)-MβCD treatments on membrane cholesterol level and cell viability of MSCs. a Cholesterol level of MSCs after treatment with MβCD for 40 min at the concentrations indicated. Membrane cholesterol levels were measured and compared (untreated cells = 100%). b Cholesterol level of MSCs after treatment with 10 mM MβCD or 100 μM chol-MβCD for the time indicated. c Time course of cholesterol level of MSCs after initial treatment with 10 mM MβCD for 60 min. After treatment, cells were washed with PBS and incubated in fresh PM containing <t>CS-FBS</t> for the time indicated. d Viability of cells, treated as described in Fig. 1b, evaluated by MTS assay. Results are presented as mean ± SD; n ≥ 3 independent experiments per cell line per time point. The results showed the effectiveness of MβCD and chol-MβCD in altering MSC membrane cholesterol level without compromising cell viability. * p
Charcoal Stripped Fbs, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Influence of cholesterol/caveolin-1/caveolae homeostasis on membrane properties and substrate adhesion characteristics of adult human mesenchymal stem cells"

Article Title: Influence of cholesterol/caveolin-1/caveolae homeostasis on membrane properties and substrate adhesion characteristics of adult human mesenchymal stem cells

Journal: Stem Cell Research & Therapy

doi: 10.1186/s13287-018-0830-4

Effects of methyl-β-cyclodextrin (MβCD) and cholesterol (chol)-MβCD treatments on membrane cholesterol level and cell viability of MSCs. a Cholesterol level of MSCs after treatment with MβCD for 40 min at the concentrations indicated. Membrane cholesterol levels were measured and compared (untreated cells = 100%). b Cholesterol level of MSCs after treatment with 10 mM MβCD or 100 μM chol-MβCD for the time indicated. c Time course of cholesterol level of MSCs after initial treatment with 10 mM MβCD for 60 min. After treatment, cells were washed with PBS and incubated in fresh PM containing CS-FBS for the time indicated. d Viability of cells, treated as described in Fig. 1b, evaluated by MTS assay. Results are presented as mean ± SD; n ≥ 3 independent experiments per cell line per time point. The results showed the effectiveness of MβCD and chol-MβCD in altering MSC membrane cholesterol level without compromising cell viability. * p
Figure Legend Snippet: Effects of methyl-β-cyclodextrin (MβCD) and cholesterol (chol)-MβCD treatments on membrane cholesterol level and cell viability of MSCs. a Cholesterol level of MSCs after treatment with MβCD for 40 min at the concentrations indicated. Membrane cholesterol levels were measured and compared (untreated cells = 100%). b Cholesterol level of MSCs after treatment with 10 mM MβCD or 100 μM chol-MβCD for the time indicated. c Time course of cholesterol level of MSCs after initial treatment with 10 mM MβCD for 60 min. After treatment, cells were washed with PBS and incubated in fresh PM containing CS-FBS for the time indicated. d Viability of cells, treated as described in Fig. 1b, evaluated by MTS assay. Results are presented as mean ± SD; n ≥ 3 independent experiments per cell line per time point. The results showed the effectiveness of MβCD and chol-MβCD in altering MSC membrane cholesterol level without compromising cell viability. * p

Techniques Used: Incubation, MTS Assay

2) Product Images from "Influence of cholesterol/caveolin-1/caveolae homeostasis on membrane properties and substrate adhesion characteristics of adult human mesenchymal stem cells"

Article Title: Influence of cholesterol/caveolin-1/caveolae homeostasis on membrane properties and substrate adhesion characteristics of adult human mesenchymal stem cells

Journal: Stem Cell Research & Therapy

doi: 10.1186/s13287-018-0830-4

Effects of methyl-β-cyclodextrin (MβCD) and cholesterol (chol)-MβCD treatments on membrane cholesterol level and cell viability of MSCs. a Cholesterol level of MSCs after treatment with MβCD for 40 min at the concentrations indicated. Membrane cholesterol levels were measured and compared (untreated cells = 100%). b Cholesterol level of MSCs after treatment with 10 mM MβCD or 100 μM chol-MβCD for the time indicated. c Time course of cholesterol level of MSCs after initial treatment with 10 mM MβCD for 60 min. After treatment, cells were washed with PBS and incubated in fresh PM containing CS-FBS for the time indicated. d Viability of cells, treated as described in Fig. 1b, evaluated by MTS assay. Results are presented as mean ± SD; n ≥ 3 independent experiments per cell line per time point. The results showed the effectiveness of MβCD and chol-MβCD in altering MSC membrane cholesterol level without compromising cell viability. * p
Figure Legend Snippet: Effects of methyl-β-cyclodextrin (MβCD) and cholesterol (chol)-MβCD treatments on membrane cholesterol level and cell viability of MSCs. a Cholesterol level of MSCs after treatment with MβCD for 40 min at the concentrations indicated. Membrane cholesterol levels were measured and compared (untreated cells = 100%). b Cholesterol level of MSCs after treatment with 10 mM MβCD or 100 μM chol-MβCD for the time indicated. c Time course of cholesterol level of MSCs after initial treatment with 10 mM MβCD for 60 min. After treatment, cells were washed with PBS and incubated in fresh PM containing CS-FBS for the time indicated. d Viability of cells, treated as described in Fig. 1b, evaluated by MTS assay. Results are presented as mean ± SD; n ≥ 3 independent experiments per cell line per time point. The results showed the effectiveness of MβCD and chol-MβCD in altering MSC membrane cholesterol level without compromising cell viability. * p

Techniques Used: Incubation, MTS Assay

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Article Snippet: Cell proliferation Following cell plating (12-well plates; ≈ 90,000 cells/well) and attachment (18 Hrs), culture medium was replaced with SDM (0.1% (v/v ) charcoal-stripped FBS (Gemini Bio-Products, Sacramento, CA) for 24 h followed by LPA treatment (0–100 μM, 24 h).

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Article Snippet: Briefly, cells were collected after 24 h in SDM (0.1% (v/v ) charcoal-stripped FBS) and added to the plastic inserts of the Transwell chamber in the absence or presence of inhibitors and 0.7 ml SDM, with or without LPA (10 μM), was added to the lower chamber.

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Article Title: Roles of intracerebral activin, inhibin, and follistatin in the regulation of Kiss-1 gene expression: Studies using primary cultures of fetal rat neuronal cells
Article Snippet: When stimulated with the test reagents, the cells were incubated with or without (control) the test reagents in high-glucose DMEM containing 1% heat-inactivated FBS and 1% penicillin-streptomycin at the indicated concentrations. .. When stimulated with E2, the cells were cultured with E2 in phenol red-free DMEM supplemented with 1% charcoal-stripped FBS (Gemini Bio-Products, West Sacramento, CA). .. 2.4 RNA preparation, reverse transcription, PCR, and quantitative real-time PCR Total RNA from stimulated cells was extracted using TRIzol-LS (Invitrogen) according to the manufacturer's instructions.

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    Gemini Bio charcoal dextran stripped fbs
    Cdc6 expression in androgen-sensitive prostate cancer cells is modulated in an androgen- and antiandrogen-dependent manner. ( A and C ) LNCaP cells were androgen-starved for 72 h and then treated with different concentrations of DHT 8 h. ( B and D ) Androgen-starved LNCaP cells were first cultured in <t>CDS–FBS</t> media with 10 nM R1881 for 24 h to elevate basal Cdc6 expression, and then were treated with different concentrations of Casodex for 8 h. Total RNA was analyzed by RT–PCR using primers specific for Cdc6 and β-actin (A and B). Bar graph represents results as the mean ± S.E. of triplicate reactions. Twenty-five micrograms of whole cell extract was probed by immunoblot with anti-Cdc6 and anti-tubulin antibodies (C and D). ( E and F ) DU145 cells were androgen-starved for 3 days and then treated with different concentrations of DHT and Casodex for 8 h. Total RNA was analyzed by RT–PCR using primers specific for Cdc6 and β-actin as outlined above.
    Charcoal Dextran Stripped Fbs, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/charcoal dextran stripped fbs/product/Gemini Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    charcoal dextran stripped fbs - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Gemini Bio charcoal stripped fbs
    Effects of methyl-β-cyclodextrin <t>(MβCD)</t> and cholesterol (chol)-MβCD treatments on membrane cholesterol level and cell viability of MSCs. a Cholesterol level of MSCs after treatment with MβCD for 40 min at the concentrations indicated. Membrane cholesterol levels were measured and compared (untreated cells = 100%). b Cholesterol level of MSCs after treatment with 10 mM MβCD or 100 μM chol-MβCD for the time indicated. c Time course of cholesterol level of MSCs after initial treatment with 10 mM MβCD for 60 min. After treatment, cells were washed with PBS and incubated in fresh PM containing <t>CS-FBS</t> for the time indicated. d Viability of cells, treated as described in Fig. 1b, evaluated by MTS assay. Results are presented as mean ± SD; n ≥ 3 independent experiments per cell line per time point. The results showed the effectiveness of MβCD and chol-MβCD in altering MSC membrane cholesterol level without compromising cell viability. * p
    Charcoal Stripped Fbs, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/charcoal stripped fbs/product/Gemini Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    charcoal stripped fbs - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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    Cdc6 expression in androgen-sensitive prostate cancer cells is modulated in an androgen- and antiandrogen-dependent manner. ( A and C ) LNCaP cells were androgen-starved for 72 h and then treated with different concentrations of DHT 8 h. ( B and D ) Androgen-starved LNCaP cells were first cultured in CDS–FBS media with 10 nM R1881 for 24 h to elevate basal Cdc6 expression, and then were treated with different concentrations of Casodex for 8 h. Total RNA was analyzed by RT–PCR using primers specific for Cdc6 and β-actin (A and B). Bar graph represents results as the mean ± S.E. of triplicate reactions. Twenty-five micrograms of whole cell extract was probed by immunoblot with anti-Cdc6 and anti-tubulin antibodies (C and D). ( E and F ) DU145 cells were androgen-starved for 3 days and then treated with different concentrations of DHT and Casodex for 8 h. Total RNA was analyzed by RT–PCR using primers specific for Cdc6 and β-actin as outlined above.

    Journal: Nucleic Acids Research

    Article Title: A novel androgen receptor-binding element modulates Cdc6 transcription in prostate cancer cells during cell-cycle progression

    doi: 10.1093/nar/gkp510

    Figure Lengend Snippet: Cdc6 expression in androgen-sensitive prostate cancer cells is modulated in an androgen- and antiandrogen-dependent manner. ( A and C ) LNCaP cells were androgen-starved for 72 h and then treated with different concentrations of DHT 8 h. ( B and D ) Androgen-starved LNCaP cells were first cultured in CDS–FBS media with 10 nM R1881 for 24 h to elevate basal Cdc6 expression, and then were treated with different concentrations of Casodex for 8 h. Total RNA was analyzed by RT–PCR using primers specific for Cdc6 and β-actin (A and B). Bar graph represents results as the mean ± S.E. of triplicate reactions. Twenty-five micrograms of whole cell extract was probed by immunoblot with anti-Cdc6 and anti-tubulin antibodies (C and D). ( E and F ) DU145 cells were androgen-starved for 3 days and then treated with different concentrations of DHT and Casodex for 8 h. Total RNA was analyzed by RT–PCR using primers specific for Cdc6 and β-actin as outlined above.

    Article Snippet: In the androgen starvation experiments, cells were grown in phenol red-free medium containing charcoal/dextran-stripped FBS (CDS–FBS, Gemini Bioproducts).

    Techniques: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction

    Effects of methyl-β-cyclodextrin (MβCD) and cholesterol (chol)-MβCD treatments on membrane cholesterol level and cell viability of MSCs. a Cholesterol level of MSCs after treatment with MβCD for 40 min at the concentrations indicated. Membrane cholesterol levels were measured and compared (untreated cells = 100%). b Cholesterol level of MSCs after treatment with 10 mM MβCD or 100 μM chol-MβCD for the time indicated. c Time course of cholesterol level of MSCs after initial treatment with 10 mM MβCD for 60 min. After treatment, cells were washed with PBS and incubated in fresh PM containing CS-FBS for the time indicated. d Viability of cells, treated as described in Fig. 1b, evaluated by MTS assay. Results are presented as mean ± SD; n ≥ 3 independent experiments per cell line per time point. The results showed the effectiveness of MβCD and chol-MβCD in altering MSC membrane cholesterol level without compromising cell viability. * p

    Journal: Stem Cell Research & Therapy

    Article Title: Influence of cholesterol/caveolin-1/caveolae homeostasis on membrane properties and substrate adhesion characteristics of adult human mesenchymal stem cells

    doi: 10.1186/s13287-018-0830-4

    Figure Lengend Snippet: Effects of methyl-β-cyclodextrin (MβCD) and cholesterol (chol)-MβCD treatments on membrane cholesterol level and cell viability of MSCs. a Cholesterol level of MSCs after treatment with MβCD for 40 min at the concentrations indicated. Membrane cholesterol levels were measured and compared (untreated cells = 100%). b Cholesterol level of MSCs after treatment with 10 mM MβCD or 100 μM chol-MβCD for the time indicated. c Time course of cholesterol level of MSCs after initial treatment with 10 mM MβCD for 60 min. After treatment, cells were washed with PBS and incubated in fresh PM containing CS-FBS for the time indicated. d Viability of cells, treated as described in Fig. 1b, evaluated by MTS assay. Results are presented as mean ± SD; n ≥ 3 independent experiments per cell line per time point. The results showed the effectiveness of MβCD and chol-MβCD in altering MSC membrane cholesterol level without compromising cell viability. * p

    Article Snippet: After MβCD pretreatment, cholesterol-depleted cells were cultured in fresh medium containing charcoal stripped FBS (CS-FBS; Gemini Bio-Products, West Sacramento, CA, USA).

    Techniques: Incubation, MTS Assay

    Effects of methyl-β-cyclodextrin (MβCD) and cholesterol (chol)-MβCD treatments on membrane cholesterol level and cell viability of MSCs. a Cholesterol level of MSCs after treatment with MβCD for 40 min at the concentrations indicated. Membrane cholesterol levels were measured and compared (untreated cells = 100%). b Cholesterol level of MSCs after treatment with 10 mM MβCD or 100 μM chol-MβCD for the time indicated. c Time course of cholesterol level of MSCs after initial treatment with 10 mM MβCD for 60 min. After treatment, cells were washed with PBS and incubated in fresh PM containing CS-FBS for the time indicated. d Viability of cells, treated as described in Fig. 1b, evaluated by MTS assay. Results are presented as mean ± SD; n ≥ 3 independent experiments per cell line per time point. The results showed the effectiveness of MβCD and chol-MβCD in altering MSC membrane cholesterol level without compromising cell viability. * p

    Journal: Stem Cell Research & Therapy

    Article Title: Influence of cholesterol/caveolin-1/caveolae homeostasis on membrane properties and substrate adhesion characteristics of adult human mesenchymal stem cells

    doi: 10.1186/s13287-018-0830-4

    Figure Lengend Snippet: Effects of methyl-β-cyclodextrin (MβCD) and cholesterol (chol)-MβCD treatments on membrane cholesterol level and cell viability of MSCs. a Cholesterol level of MSCs after treatment with MβCD for 40 min at the concentrations indicated. Membrane cholesterol levels were measured and compared (untreated cells = 100%). b Cholesterol level of MSCs after treatment with 10 mM MβCD or 100 μM chol-MβCD for the time indicated. c Time course of cholesterol level of MSCs after initial treatment with 10 mM MβCD for 60 min. After treatment, cells were washed with PBS and incubated in fresh PM containing CS-FBS for the time indicated. d Viability of cells, treated as described in Fig. 1b, evaluated by MTS assay. Results are presented as mean ± SD; n ≥ 3 independent experiments per cell line per time point. The results showed the effectiveness of MβCD and chol-MβCD in altering MSC membrane cholesterol level without compromising cell viability. * p

    Article Snippet: After MβCD pretreatment, cholesterol-depleted cells were cultured in fresh medium containing charcoal stripped FBS (CS-FBS; Gemini Bio-Products, West Sacramento, CA, USA).

    Techniques: Incubation, MTS Assay