chambered cell culture slides permanox  (Thermo Fisher)


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    Structured Review

    Thermo Fisher chambered cell culture slides permanox
    Chambered Cell Culture Slides Permanox, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chambered cell culture slides permanox/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chambered cell culture slides permanox - by Bioz Stars, 2020-09
    85/100 stars

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    Western Blot:

    Article Title: Endothelial transcytosis of myeloperoxidase confers specificity to vascular ECM proteins as targets of tyrosine nitration
    Article Snippet: .. Lymphocyte separation medium was obtained from Organon Teknika (Durham, North Carolina, USA), TRITC-labeled dextran (4,400 Da) and zymosan from Sigma Chemical Co. (St. Louis, Missouri, USA), Transwell cell culture inserts from Becton Dickinson and Co. (Franklin Lakes, New Jersey, USA), chambered cell culture slides (Permanox) from Nalge NUNC International (Rochester, New York, USA), gradient gels from Bio-Rad Laboratories (Hercules, California, USA), and enhanced chemiluminescence (Femto SuperSignal) for Western blot analysis from Pierce Chemical Co. (Rockford, Illinois, USA). .. MPO activity in cell lysates was determined by adding an aliquot of cell lysate to 43 mM NaH2 PO4 (pH 5.4), 1.2 mM tetramethylbenzidine, and 100 μM H2 O2 .

    Cell Culture:

    Article Title: Endothelial transcytosis of myeloperoxidase confers specificity to vascular ECM proteins as targets of tyrosine nitration
    Article Snippet: .. Lymphocyte separation medium was obtained from Organon Teknika (Durham, North Carolina, USA), TRITC-labeled dextran (4,400 Da) and zymosan from Sigma Chemical Co. (St. Louis, Missouri, USA), Transwell cell culture inserts from Becton Dickinson and Co. (Franklin Lakes, New Jersey, USA), chambered cell culture slides (Permanox) from Nalge NUNC International (Rochester, New York, USA), gradient gels from Bio-Rad Laboratories (Hercules, California, USA), and enhanced chemiluminescence (Femto SuperSignal) for Western blot analysis from Pierce Chemical Co. (Rockford, Illinois, USA). .. MPO activity in cell lysates was determined by adding an aliquot of cell lysate to 43 mM NaH2 PO4 (pH 5.4), 1.2 mM tetramethylbenzidine, and 100 μM H2 O2 .

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    Thermo Fisher nunc lab tek chamber slide system
    Effect of metformin on hepatic mitochondrial biogenesis. A) Mouse primary hepatocytes were cultured on <t>permanox</t> <t>Lab-Tek</t> 8-well chamber slides with 1 mM metformin for 48 hours. Cells were fixed with 4% PFA, immunostained as described and mounted with Fluoroshield with DAPI. MTCO1 stained green, cell nuclei blue. Representative images are shown. B) Western blot of whole cell lysates from mouse primary hepatocytes incubated with or without metformin for 48 hours. Upper panel: immunostaining with anti-MTCO1; lower panel: β-actin was detected as a loading control. C) HepG2 cells were cultured on permanox Lab-Tek 8-well chamber slides with 3 mM metformin for 48 hours, incubated with Mitotracker Green FM (100 nM) for 1 hour and mounted with Fluoroshield with DAPI. Mitochondria stained green, cell nuclei blue. Representative images are shown. D) Mitochondrial mass of HepG2 cells, treated with 3 mM metformin for 48 hours, was measured by FACS and compared with untreated cells by fluorescence levels upon staining with MitoTracker Green FM. A representative experiment is shown, which was repeated two additional times with similar results. MFI (Mean Fluorescence Intensity), ANOVA, Neuman–Keuls post-hoc test, *** P
    Nunc Lab Tek Chamber Slide System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc lab tek chamber slide system/product/Thermo Fisher
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    nunc lab tek chamber slide system - by Bioz Stars, 2020-09
    99/100 stars
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    90
    Thermo Fisher lab tek 8 well permanox chamber slides
    Effect of metformin on hepatic mitochondrial biogenesis. A) Mouse primary hepatocytes were cultured on <t>permanox</t> <t>Lab-Tek</t> 8-well chamber slides with 1 mM metformin for 48 hours. Cells were fixed with 4% PFA, immunostained as described and mounted with Fluoroshield with DAPI. MTCO1 stained green, cell nuclei blue. Representative images are shown. B) Western blot of whole cell lysates from mouse primary hepatocytes incubated with or without metformin for 48 hours. Upper panel: immunostaining with anti-MTCO1; lower panel: β-actin was detected as a loading control. C) HepG2 cells were cultured on permanox Lab-Tek 8-well chamber slides with 3 mM metformin for 48 hours, incubated with Mitotracker Green FM (100 nM) for 1 hour and mounted with Fluoroshield with DAPI. Mitochondria stained green, cell nuclei blue. Representative images are shown. D) Mitochondrial mass of HepG2 cells, treated with 3 mM metformin for 48 hours, was measured by FACS and compared with untreated cells by fluorescence levels upon staining with MitoTracker Green FM. A representative experiment is shown, which was repeated two additional times with similar results. MFI (Mean Fluorescence Intensity), ANOVA, Neuman–Keuls post-hoc test, *** P
    Lab Tek 8 Well Permanox Chamber Slides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lab tek 8 well permanox chamber slides/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lab tek 8 well permanox chamber slides - by Bioz Stars, 2020-09
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    86
    Thermo Fisher gelatin coated four well tissue culture permanox chamber slides
    Effect of metformin on hepatic mitochondrial biogenesis. A) Mouse primary hepatocytes were cultured on <t>permanox</t> <t>Lab-Tek</t> 8-well chamber slides with 1 mM metformin for 48 hours. Cells were fixed with 4% PFA, immunostained as described and mounted with Fluoroshield with DAPI. MTCO1 stained green, cell nuclei blue. Representative images are shown. B) Western blot of whole cell lysates from mouse primary hepatocytes incubated with or without metformin for 48 hours. Upper panel: immunostaining with anti-MTCO1; lower panel: β-actin was detected as a loading control. C) HepG2 cells were cultured on permanox Lab-Tek 8-well chamber slides with 3 mM metformin for 48 hours, incubated with Mitotracker Green FM (100 nM) for 1 hour and mounted with Fluoroshield with DAPI. Mitochondria stained green, cell nuclei blue. Representative images are shown. D) Mitochondrial mass of HepG2 cells, treated with 3 mM metformin for 48 hours, was measured by FACS and compared with untreated cells by fluorescence levels upon staining with MitoTracker Green FM. A representative experiment is shown, which was repeated two additional times with similar results. MFI (Mean Fluorescence Intensity), ANOVA, Neuman–Keuls post-hoc test, *** P
    Gelatin Coated Four Well Tissue Culture Permanox Chamber Slides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gelatin coated four well tissue culture permanox chamber slides/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gelatin coated four well tissue culture permanox chamber slides - by Bioz Stars, 2020-09
    86/100 stars
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    Effect of metformin on hepatic mitochondrial biogenesis. A) Mouse primary hepatocytes were cultured on permanox Lab-Tek 8-well chamber slides with 1 mM metformin for 48 hours. Cells were fixed with 4% PFA, immunostained as described and mounted with Fluoroshield with DAPI. MTCO1 stained green, cell nuclei blue. Representative images are shown. B) Western blot of whole cell lysates from mouse primary hepatocytes incubated with or without metformin for 48 hours. Upper panel: immunostaining with anti-MTCO1; lower panel: β-actin was detected as a loading control. C) HepG2 cells were cultured on permanox Lab-Tek 8-well chamber slides with 3 mM metformin for 48 hours, incubated with Mitotracker Green FM (100 nM) for 1 hour and mounted with Fluoroshield with DAPI. Mitochondria stained green, cell nuclei blue. Representative images are shown. D) Mitochondrial mass of HepG2 cells, treated with 3 mM metformin for 48 hours, was measured by FACS and compared with untreated cells by fluorescence levels upon staining with MitoTracker Green FM. A representative experiment is shown, which was repeated two additional times with similar results. MFI (Mean Fluorescence Intensity), ANOVA, Neuman–Keuls post-hoc test, *** P

    Journal: PLoS ONE

    Article Title: Metformin Reduces Hepatic Expression of SIRT3, the Mitochondrial Deacetylase Controlling Energy Metabolism

    doi: 10.1371/journal.pone.0049863

    Figure Lengend Snippet: Effect of metformin on hepatic mitochondrial biogenesis. A) Mouse primary hepatocytes were cultured on permanox Lab-Tek 8-well chamber slides with 1 mM metformin for 48 hours. Cells were fixed with 4% PFA, immunostained as described and mounted with Fluoroshield with DAPI. MTCO1 stained green, cell nuclei blue. Representative images are shown. B) Western blot of whole cell lysates from mouse primary hepatocytes incubated with or without metformin for 48 hours. Upper panel: immunostaining with anti-MTCO1; lower panel: β-actin was detected as a loading control. C) HepG2 cells were cultured on permanox Lab-Tek 8-well chamber slides with 3 mM metformin for 48 hours, incubated with Mitotracker Green FM (100 nM) for 1 hour and mounted with Fluoroshield with DAPI. Mitochondria stained green, cell nuclei blue. Representative images are shown. D) Mitochondrial mass of HepG2 cells, treated with 3 mM metformin for 48 hours, was measured by FACS and compared with untreated cells by fluorescence levels upon staining with MitoTracker Green FM. A representative experiment is shown, which was repeated two additional times with similar results. MFI (Mean Fluorescence Intensity), ANOVA, Neuman–Keuls post-hoc test, *** P

    Article Snippet: Permanox Lab-Tek™ Chamber Slides (#177445) were obtained from Thermo Fisher Scientific (Walthman, MA, USA), Mitotracker Green FM from Invitrogen (Carlsbad, CA, USA) and Any-kDa precast gels from Bio-Rad (Hercules, CA, USA).

    Techniques: Cell Culture, Staining, Western Blot, Incubation, Immunostaining, FACS, Fluorescence