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( A ) Representative PSCs in response to 10 light pulses at 10 Hz recorded from GC at holding potentials of –30 mV before (black) and after application of 10 μM NBQX (blue). The EPSC (red) was isolated by subtracting the blue trace from the black trace. 1 μM TTX, 500 μM 4-AP, 50 μM D-AP5, 100 μM LY341495, and 3 μM <t>CGP55845</t> were included in the extracellular solution throughout the experiments. ( B ) Summary graph of normalized EPSC and IPSC amplitudes plotted against stimulus number. Two-way repeated measures ANOVA, F (1,7) = 17.5, p<0.01, n=8. Data are presented as mean ± SEM. **p<0.01. Figure 5—figure supplement 1—source data 1. Source data displayed on .
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( A ) Representative PSCs in response to 10 light pulses at 10 Hz recorded from GC at holding potentials of –30 mV before (black) and after application of 10 μM NBQX (blue). The EPSC (red) was isolated by subtracting the blue trace from the black trace. 1 μM TTX, 500 μM 4-AP, 50 μM D-AP5, 100 μM LY341495, and 3 μM <t>CGP55845</t> were included in the extracellular solution throughout the experiments. ( B ) Summary graph of normalized EPSC and IPSC amplitudes plotted against stimulus number. Two-way repeated measures ANOVA, F (1,7) = 17.5, p<0.01, n=8. Data are presented as mean ± SEM. **p<0.01. Figure 5—figure supplement 1—source data 1. Source data displayed on .
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( A ) Representative PSCs in response to 10 light pulses at 10 Hz recorded from GC at holding potentials of –30 mV before (black) and after application of 10 μM NBQX (blue). The EPSC (red) was isolated by subtracting the blue trace from the black trace. 1 μM TTX, 500 μM 4-AP, 50 μM D-AP5, 100 μM LY341495, and 3 μM CGP55845 were included in the extracellular solution throughout the experiments. ( B ) Summary graph of normalized EPSC and IPSC amplitudes plotted against stimulus number. Two-way repeated measures ANOVA, F (1,7) = 17.5, p<0.01, n=8. Data are presented as mean ± SEM. **p<0.01. Figure 5—figure supplement 1—source data 1. Source data displayed on .

Journal: eLife

Article Title: Distinct release properties of glutamate/GABA co-transmission serve as a frequency-dependent filtering of supramammillary inputs

doi: 10.7554/eLife.99711

Figure Lengend Snippet: ( A ) Representative PSCs in response to 10 light pulses at 10 Hz recorded from GC at holding potentials of –30 mV before (black) and after application of 10 μM NBQX (blue). The EPSC (red) was isolated by subtracting the blue trace from the black trace. 1 μM TTX, 500 μM 4-AP, 50 μM D-AP5, 100 μM LY341495, and 3 μM CGP55845 were included in the extracellular solution throughout the experiments. ( B ) Summary graph of normalized EPSC and IPSC amplitudes plotted against stimulus number. Two-way repeated measures ANOVA, F (1,7) = 17.5, p<0.01, n=8. Data are presented as mean ± SEM. **p<0.01. Figure 5—figure supplement 1—source data 1. Source data displayed on .

Article Snippet: CGP55845 was purchased from Hello Bio.

Techniques: Isolation