Journal: Neural Regeneration Research

Article Title: Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury

doi: 10.4103/NRR.NRR-D-23-01256

Figure Lengend Snippet: Antibodies used for immunostaining

Article Snippet: Rabbit anti-cGAS , 1: 1500 , Proteintech , 26416-1-AP , AB_2880507.

Techniques:

Journal: Neural Regeneration Research

Article Title: Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury

doi: 10.4103/NRR.NRR-D-23-01256

Figure Lengend Snippet: Antibodies used for western blotting

Article Snippet: Rabbit anti-cGAS , 1: 1500 , Proteintech , 26416-1-AP , AB_2880507.

Techniques: Western Blot

Journal: Neural Regeneration Research

Article Title: The cGAS-STING-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinson’s disease

doi: 10.4103/NRR.NRR-D-23-01684

Figure Lengend Snippet: The cGAS-STING pathway plays a pivotal role in regulating IRF7 activation and nuclear translocation. (A) Western blot analysis of the expression of key molecules (cGAS, p-STING, and TBK1) in the cGAS-STING-TBK1 pathway within the striatum of MPTP-treated mice ( n = 6 per group). (B, C) BV2 cells were pre-treated with either RU.521 (2 µM) or H151 (5 µM) for 12 hours before being exposed to MPP + for an additional 24 hours, and western blotting was performed to determine the relative protein levels of p-IRF7 and IRF7 in these cells ( n = 6 independent experiments). (D) Fluorescence staining for nuclei (blue: DAPI) and p-IRF7 (red: Cy3). p-IRF7 localization to the nuclei was assessed by fluorescence microscopy. The percentage of p-IRF7 nuclear translocation in the MPP + -induced group was significantly higher than that in the control group, and pre-treatment with H151 significantly reduced the percentage of MPP + -induced p-IRF7 nuclear translocation. Arrows indicate nuclear translocation; scale bars: 20 μm. The percentage of cells with significant IRF7 nuclear translocation was calculated from multiple randomly selected fields of view (control samples: three fields of view, at least 200 cells; MPP + stimulated samples: three fields of view, at least 200 cells) ( n = 3 independent experiments). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 (unpaired t -test [A] or one-way analysis of variance followed by Tukey’s post hoc test [B–D]). cGAS: Cyclic guanosine monophosphate adenosine monophosphate synthase; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; H-151: a STING inhibitor; IRF7: interferon regulatory factor 7; MPP + : 1-methyl-4-phenylpyridinium; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin; ns: not significant; p-IRF7: phospho-IRF7; p-STING: phospho-STING; RU.521: a selective cGAS inhibitor; STING: stimulator of interferon genes; TBK1: TANK-binding kinase 1.

Article Snippet: To investigate the potential neuroprotective effects of cGAS or STING inhibition, cells were pre-incubated with the selective cGAS inhibitor RU.521 (2 μM; Cat# HY-114180, MCE) or the STING inhibitor H151 (5 μM; Cat# HY-112693, MCE) for 12 hours before MPP + (20 μM) exposure (Hinkle et al., 2022; Wu et al., 2023).

Techniques: Activation Assay, Translocation Assay, Western Blot, Expressing, Fluorescence, Staining, Microscopy, Control, Binding Assay