cfx96 real time pcr detection system (Bio-Rad)
Name:
CFX96 Touch Real Time PCR Detection System with Starter Package
Description:
Modular thermal cycler platform includes C1000 Touch Thermal Cycler Chassis CFX96 Optical Reaction Module CFX Maestro Software qbase Software license cables reagents consumables
Catalog Number:
1855196
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None
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Modular thermal cycler platform includes C1000 Touch Thermal Cycler Chassis CFX96 Optical Reaction Module CFX Maestro Software qbase Software license cables reagents consumables
https://www.bioz.com/result/cfx96 real time pcr detection system/product/Bio-Rad
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1) Product Images from "Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿ †"
Article Title: Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿ †
Journal: Journal of Clinical Microbiology
doi: 10.1128/JCM.01142-10

Figure Legend Snippet: Highly specific NA signature primers for the 2009 H1N1 pandemic influenza virus. (A) Amplification of pandemic 2009 H1N1 NA in a conventional RT-PCR assay. RNA extracted from 1 × 10 7 to 1 × 10 8 PFU/ml of the shown H1N1 or H3N2 influenza viruses was subjected to a one-step RT-PCR using either the 2009 H1N1 NA signature primers (upper panel) or M segment consensus primers (lower panel) and run on a 1.2% agarose gel for detection by ethidium bromide. (B) Performance of 2009 H1N1 NA signature primers in a SYBR green assay. Specific NA signal curves obtained in a SYBR green one-step RT-PCR assay for the reference strains are shown. (C) Melting curve confirmation of specific products. In panels B and C, each sample was run in triplicate in a 96-well plate (25-μl final volume) using a Bio-Rad CFX96 real-time PCR detection system. Data are representative of two independent experiments with identical results.
Techniques Used: Amplification, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, SYBR Green Assay, Real-time Polymerase Chain Reaction
2) Product Images from "Performance Evaluation of the Real-Q Cytomegalovirus (CMV) Quantification Kit Using Two Real-Time PCR Systems for Quantifying CMV DNA in Whole Blood"
Article Title: Performance Evaluation of the Real-Q Cytomegalovirus (CMV) Quantification Kit Using Two Real-Time PCR Systems for Quantifying CMV DNA in Whole Blood
Journal: Annals of Laboratory Medicine
doi: 10.3343/alm.2016.36.6.603

Figure Legend Snippet: Bland-Altman plot comparing the artus cytomegalovirus (CMV) RG PCR kit and the Real-Q CMV Quantification kit on different real-time PCR platforms: (A) the 7500 Fast Real-time PCR system, (B) the CFX96 real-time PCR detection system. Solid lines are the mean differences between the values; dashed lines are the mean difference plus or minus 1.96 SD (95% confidential interval of mean difference).
Techniques Used: Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

Figure Legend Snippet: Linearity of the Real-Q cytomegalovirus (CMV) Quantification kit on different real-time PCR platforms: (A) the 7500 Fast real-time PCR system and (B) the CFX96 real-time PCR detection system.
Techniques Used: Real-time Polymerase Chain Reaction
3) Product Images from "Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿ †"
Article Title: Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿ †
Journal: Journal of Clinical Microbiology
doi: 10.1128/JCM.01142-10

Figure Legend Snippet: Highly specific NA signature primers for the 2009 H1N1 pandemic influenza virus. (A) Amplification of pandemic 2009 H1N1 NA in a conventional RT-PCR assay. RNA extracted from 1 × 10 7 to 1 × 10 8 PFU/ml of the shown H1N1 or H3N2 influenza viruses was subjected to a one-step RT-PCR using either the 2009 H1N1 NA signature primers (upper panel) or M segment consensus primers (lower panel) and run on a 1.2% agarose gel for detection by ethidium bromide. (B) Performance of 2009 H1N1 NA signature primers in a SYBR green assay. Specific NA signal curves obtained in a SYBR green one-step RT-PCR assay for the reference strains are shown. (C) Melting curve confirmation of specific products. In panels B and C, each sample was run in triplicate in a 96-well plate (25-μl final volume) using a Bio-Rad CFX96 real-time PCR detection system. Data are representative of two independent experiments with identical results.
Techniques Used: Amplification, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, SYBR Green Assay, Real-time Polymerase Chain Reaction
4) Product Images from "The Extracellular IFI16 Protein Propagates Inflammation in Endothelial Cells Via p38 MAPK and NF-κB p65 Activation"
Article Title: The Extracellular IFI16 Protein Propagates Inflammation in Endothelial Cells Via p38 MAPK and NF-κB p65 Activation
Journal: Journal of Interferon & Cytokine Research
doi: 10.1089/jir.2014.0168

Figure Legend Snippet: mRNA expression profile of proinflammatory cytokines in rIFI16-stimulated HUVEC. (A) Bar graphs representing folds change mRNA expression by HUVEC grown in presence/absence of VEGF growth factor in EGM-2, stimulated for 24 h with either rIFI16 (50 μg/mL) or mock control. (B) Solid and dotted line histograms representing fold change time-dependent mRNA expression by HUVEC grown in VEGF-depleted EGM-2, stimulated with either rIFI16 (50 μg/mL) or mock control for 0, 4, 12, 24, 48, and 72 h. Each real time PCR reaction was performed with Bio-Rad CFX96 and relative normalized expression of mRNA was calculated by the comparative Ct method. All experiments were performed in triplicates and the bar graphs/histograms represent (mean±SD) 6 independent experiments. All experimental data were processed by GraphPad Prism 6.01 software that was used to plot histograms and calculate statistical significance by unpaired t -test. * P
Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Software

Figure Legend Snippet: rIFI16 synergizes with lipopolysaccharide (LPS) to increase proinflammatory cytokine expression through MyD88-dependent pathways. (A) HUVEC were grown in VEGF-depleted EGM-2 and stimulated for 24 h with either mock control, rIFI16 (50 μg/mL), LPS (10 ng/mL), or rIFI16+LPS mixture. Each RT-PCR reaction was performed with Bio-Rad CFX96 and relative normalized expression of mRNA was calculated using comparative Ct method. (B) MyD88/TLR4 silencing/neutralization inhibits rIFI16 cytokine stimulating activity and LPS synergy. HUVEC were stimulated for 24 h with mock control, rIFI16 (50 μg/mL), LPS (10 ng/mL), and rIFI16+LPS in the presence of no siRNA/antibodies (Control dataset), siRNA control, siRNA MyD88, Control IgG, or anti-TLR antibodies. (C) Flow cytometry histograms showing expression of ICAM1, VCAM1, and TLR4 in HUVEC when stimulated with mock control, rIFI16 (50 μg/mL), or LPS (10 ng/mL). One representative example from 6 independent experiments is shown. The shaded histograms represent binding of anti-ICAM1, anti-VCAM1, and anti-TLR4 antibodies and open histograms represent corresponding untreated controls. Isotype controls for each antibody were used in parallel to identify unspecific staining. (D) Histograms representing% positive cells after treatment and normalized mean fluorescence intensity as compared to isotype controls, plotted as mean±SD for ICAM1, VCAM1, and TLR4 from 6 independent experiments. All experimental data were processed by GraphPad Prism 6.01 software that was used to plot histograms and calculate statistical significance by 2-way ANOVA. * P
Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Neutralization, Activity Assay, Flow Cytometry, Cytometry, Binding Assay, Staining, Fluorescence, Software
5) Product Images from "Simultaneous detection of duck circovirus and novel goose parvovirus via SYBR green I-based duplex real-time polymerase chain reaction analysis"
Article Title: Simultaneous detection of duck circovirus and novel goose parvovirus via SYBR green I-based duplex real-time polymerase chain reaction analysis
Journal: Molecular and Cellular Probes
doi: 10.1016/j.mcp.2020.101648

Figure Legend Snippet: Standard SYBR Green I-based quantitative PCR curves generated by plotting the mean Cq values from triplicate samples versus the concentrations of DuCV plasmid DNA standards, which were serially diluted 10-fold over concentrations ranging from 1 × 10 10 to 1 × 10 3 copies/μL. The coefficient of determination (R 2 ) and the line equation of the regression curve (y) were calculated using a CFX96™ Real-Time PCR Detection System. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Techniques Used: SYBR Green Assay, Real-time Polymerase Chain Reaction, Generated, Plasmid Preparation

Figure Legend Snippet: Standard SYBR Green I-based quantitative PCR curves generated by plotting the mean Cq values from triplicate samples versus the concentrations of NGPV plasmid DNA standards, which were serially diluted 10-fold over concentrations ranging from 1 × 10 10 to 1 × 10 3 copies/μL. The coefficient of determination (R 2 ) and the line equation of the regression curve (y) were calculated using a CFX96™ Real-Time PCR Detection System. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Techniques Used: SYBR Green Assay, Real-time Polymerase Chain Reaction, Generated, Plasmid Preparation
6) Product Images from "Identification, gene expression and genetic polymorphism of zinc finger A20/AN1 stress-associated genes, HvSAP, in salt stressed barley from Kazakhstan"
Article Title: Identification, gene expression and genetic polymorphism of zinc finger A20/AN1 stress-associated genes, HvSAP, in salt stressed barley from Kazakhstan
Journal: BMC Plant Biology
doi: 10.1186/s12870-020-02332-4

Figure Legend Snippet: Allele discrimination for the Amplifluor-like SNP marker KATU-B30. Fifty F 3 breeding lines from the segregating population Granal × Baisheshek were used. Parental genotypes are designated as: ♀ P 1 , Granal, and ♂ P 2 , Baisheshek. Relative Fluorescence Units (RFU) for fluorophores FAM and HEX were transformed automatically into genotyping of alleles 1 and 2, respectively, using a BioRad CFX96 Real-Time PCR Detection System Instrument
Techniques Used: Marker, Fluorescence, Transformation Assay, Real-time Polymerase Chain Reaction
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The fluorescent probe-based 2009 H1N1 NA signature-specific assay was conducted with the same cycling conditions and volumes described above for either the Expressing:Article Title: Effects of Long-Term Supplementation of Blue-Green Algae on Lipid Metabolism in C57BL/6J mice Article Snippet: .. qRT-PCR analysis for gene expression was conducted as previously described using the SYBR Green procedure and Reverse Transcription Polymerase Chain Reaction:Article Title: BCL-XL expression is essential for human erythropoiesis and engraftment of hematopoietic stem cells Article Snippet: .. Real time Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed by using BIO-RAD (CFX96 Touch) Western Blot:Article Title: Performance Evaluation of the Real-Q Cytomegalovirus (CMV) Quantification Kit Using Two Real-Time PCR Systems for Quantifying CMV DNA in Whole Blood Article Snippet: .. 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