cfx96 real time pcr detection system  (Bio-Rad)

 
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    CFX96 Touch Real Time PCR Detection System with Starter Package
    Description:
    Modular thermal cycler platform includes C1000 Touch Thermal Cycler Chassis CFX96 Optical Reaction Module CFX Maestro Software qbase Software license cables reagents consumables
    Catalog Number:
    1855196
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    Structured Review

    Bio-Rad cfx96 real time pcr detection system
    CFX96 Touch Real Time PCR Detection System with Starter Package
    Modular thermal cycler platform includes C1000 Touch Thermal Cycler Chassis CFX96 Optical Reaction Module CFX Maestro Software qbase Software license cables reagents consumables
    https://www.bioz.com/result/cfx96 real time pcr detection system/product/Bio-Rad
    Average 99 stars, based on 1430 article reviews
    Price from $9.99 to $1999.99
    cfx96 real time pcr detection system - by Bioz Stars, 2021-01
    99/100 stars

    Images

    1) Product Images from "Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿ †"

    Article Title: Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿ †

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.01142-10

    Highly specific NA signature primers for the 2009 H1N1 pandemic influenza virus. (A) Amplification of pandemic 2009 H1N1 NA in a conventional RT-PCR assay. RNA extracted from 1 × 10 7 to 1 × 10 8 PFU/ml of the shown H1N1 or H3N2 influenza viruses was subjected to a one-step RT-PCR using either the 2009 H1N1 NA signature primers (upper panel) or M segment consensus primers (lower panel) and run on a 1.2% agarose gel for detection by ethidium bromide. (B) Performance of 2009 H1N1 NA signature primers in a SYBR green assay. Specific NA signal curves obtained in a SYBR green one-step RT-PCR assay for the reference strains are shown. (C) Melting curve confirmation of specific products. In panels B and C, each sample was run in triplicate in a 96-well plate (25-μl final volume) using a Bio-Rad CFX96 real-time PCR detection system. Data are representative of two independent experiments with identical results.
    Figure Legend Snippet: Highly specific NA signature primers for the 2009 H1N1 pandemic influenza virus. (A) Amplification of pandemic 2009 H1N1 NA in a conventional RT-PCR assay. RNA extracted from 1 × 10 7 to 1 × 10 8 PFU/ml of the shown H1N1 or H3N2 influenza viruses was subjected to a one-step RT-PCR using either the 2009 H1N1 NA signature primers (upper panel) or M segment consensus primers (lower panel) and run on a 1.2% agarose gel for detection by ethidium bromide. (B) Performance of 2009 H1N1 NA signature primers in a SYBR green assay. Specific NA signal curves obtained in a SYBR green one-step RT-PCR assay for the reference strains are shown. (C) Melting curve confirmation of specific products. In panels B and C, each sample was run in triplicate in a 96-well plate (25-μl final volume) using a Bio-Rad CFX96 real-time PCR detection system. Data are representative of two independent experiments with identical results.

    Techniques Used: Amplification, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, SYBR Green Assay, Real-time Polymerase Chain Reaction

    2) Product Images from "Performance Evaluation of the Real-Q Cytomegalovirus (CMV) Quantification Kit Using Two Real-Time PCR Systems for Quantifying CMV DNA in Whole Blood"

    Article Title: Performance Evaluation of the Real-Q Cytomegalovirus (CMV) Quantification Kit Using Two Real-Time PCR Systems for Quantifying CMV DNA in Whole Blood

    Journal: Annals of Laboratory Medicine

    doi: 10.3343/alm.2016.36.6.603

    Bland-Altman plot comparing the artus cytomegalovirus (CMV) RG PCR kit and the Real-Q CMV Quantification kit on different real-time PCR platforms: (A) the 7500 Fast Real-time PCR system, (B) the CFX96 real-time PCR detection system. Solid lines are the mean differences between the values; dashed lines are the mean difference plus or minus 1.96 SD (95% confidential interval of mean difference).
    Figure Legend Snippet: Bland-Altman plot comparing the artus cytomegalovirus (CMV) RG PCR kit and the Real-Q CMV Quantification kit on different real-time PCR platforms: (A) the 7500 Fast Real-time PCR system, (B) the CFX96 real-time PCR detection system. Solid lines are the mean differences between the values; dashed lines are the mean difference plus or minus 1.96 SD (95% confidential interval of mean difference).

    Techniques Used: Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    Linearity of the Real-Q cytomegalovirus (CMV) Quantification kit on different real-time PCR platforms: (A) the 7500 Fast real-time PCR system and (B) the CFX96 real-time PCR detection system.
    Figure Legend Snippet: Linearity of the Real-Q cytomegalovirus (CMV) Quantification kit on different real-time PCR platforms: (A) the 7500 Fast real-time PCR system and (B) the CFX96 real-time PCR detection system.

    Techniques Used: Real-time Polymerase Chain Reaction

    3) Product Images from "Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿ †"

    Article Title: Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿ †

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.01142-10

    Highly specific NA signature primers for the 2009 H1N1 pandemic influenza virus. (A) Amplification of pandemic 2009 H1N1 NA in a conventional RT-PCR assay. RNA extracted from 1 × 10 7 to 1 × 10 8 PFU/ml of the shown H1N1 or H3N2 influenza viruses was subjected to a one-step RT-PCR using either the 2009 H1N1 NA signature primers (upper panel) or M segment consensus primers (lower panel) and run on a 1.2% agarose gel for detection by ethidium bromide. (B) Performance of 2009 H1N1 NA signature primers in a SYBR green assay. Specific NA signal curves obtained in a SYBR green one-step RT-PCR assay for the reference strains are shown. (C) Melting curve confirmation of specific products. In panels B and C, each sample was run in triplicate in a 96-well plate (25-μl final volume) using a Bio-Rad CFX96 real-time PCR detection system. Data are representative of two independent experiments with identical results.
    Figure Legend Snippet: Highly specific NA signature primers for the 2009 H1N1 pandemic influenza virus. (A) Amplification of pandemic 2009 H1N1 NA in a conventional RT-PCR assay. RNA extracted from 1 × 10 7 to 1 × 10 8 PFU/ml of the shown H1N1 or H3N2 influenza viruses was subjected to a one-step RT-PCR using either the 2009 H1N1 NA signature primers (upper panel) or M segment consensus primers (lower panel) and run on a 1.2% agarose gel for detection by ethidium bromide. (B) Performance of 2009 H1N1 NA signature primers in a SYBR green assay. Specific NA signal curves obtained in a SYBR green one-step RT-PCR assay for the reference strains are shown. (C) Melting curve confirmation of specific products. In panels B and C, each sample was run in triplicate in a 96-well plate (25-μl final volume) using a Bio-Rad CFX96 real-time PCR detection system. Data are representative of two independent experiments with identical results.

    Techniques Used: Amplification, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, SYBR Green Assay, Real-time Polymerase Chain Reaction

    4) Product Images from "The Extracellular IFI16 Protein Propagates Inflammation in Endothelial Cells Via p38 MAPK and NF-κB p65 Activation"

    Article Title: The Extracellular IFI16 Protein Propagates Inflammation in Endothelial Cells Via p38 MAPK and NF-κB p65 Activation

    Journal: Journal of Interferon & Cytokine Research

    doi: 10.1089/jir.2014.0168

    mRNA expression profile of proinflammatory cytokines in rIFI16-stimulated HUVEC. (A) Bar graphs representing folds change mRNA expression by HUVEC grown in presence/absence of VEGF growth factor in EGM-2, stimulated for 24 h with either rIFI16 (50 μg/mL) or mock control. (B) Solid and dotted line histograms representing fold change time-dependent mRNA expression by HUVEC grown in VEGF-depleted EGM-2, stimulated with either rIFI16 (50 μg/mL) or mock control for 0, 4, 12, 24, 48, and 72 h. Each real time PCR reaction was performed with Bio-Rad CFX96 and relative normalized expression of mRNA was calculated by the comparative Ct method. All experiments were performed in triplicates and the bar graphs/histograms represent (mean±SD) 6 independent experiments. All experimental data were processed by GraphPad Prism 6.01 software that was used to plot histograms and calculate statistical significance by unpaired t -test. * P
    Figure Legend Snippet: mRNA expression profile of proinflammatory cytokines in rIFI16-stimulated HUVEC. (A) Bar graphs representing folds change mRNA expression by HUVEC grown in presence/absence of VEGF growth factor in EGM-2, stimulated for 24 h with either rIFI16 (50 μg/mL) or mock control. (B) Solid and dotted line histograms representing fold change time-dependent mRNA expression by HUVEC grown in VEGF-depleted EGM-2, stimulated with either rIFI16 (50 μg/mL) or mock control for 0, 4, 12, 24, 48, and 72 h. Each real time PCR reaction was performed with Bio-Rad CFX96 and relative normalized expression of mRNA was calculated by the comparative Ct method. All experiments were performed in triplicates and the bar graphs/histograms represent (mean±SD) 6 independent experiments. All experimental data were processed by GraphPad Prism 6.01 software that was used to plot histograms and calculate statistical significance by unpaired t -test. * P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Software

    rIFI16 synergizes with lipopolysaccharide (LPS) to increase proinflammatory cytokine expression through MyD88-dependent pathways. (A) HUVEC were grown in VEGF-depleted EGM-2 and stimulated for 24 h with either mock control, rIFI16 (50 μg/mL), LPS (10 ng/mL), or rIFI16+LPS mixture. Each RT-PCR reaction was performed with Bio-Rad CFX96 and relative normalized expression of mRNA was calculated using comparative Ct method. (B) MyD88/TLR4 silencing/neutralization inhibits rIFI16 cytokine stimulating activity and LPS synergy. HUVEC were stimulated for 24 h with mock control, rIFI16 (50 μg/mL), LPS (10 ng/mL), and rIFI16+LPS in the presence of no siRNA/antibodies (Control dataset), siRNA control, siRNA MyD88, Control IgG, or anti-TLR antibodies. (C) Flow cytometry histograms showing expression of ICAM1, VCAM1, and TLR4 in HUVEC when stimulated with mock control, rIFI16 (50 μg/mL), or LPS (10 ng/mL). One representative example from 6 independent experiments is shown. The shaded histograms represent binding of anti-ICAM1, anti-VCAM1, and anti-TLR4 antibodies and open histograms represent corresponding untreated controls. Isotype controls for each antibody were used in parallel to identify unspecific staining. (D) Histograms representing% positive cells after treatment and normalized mean fluorescence intensity as compared to isotype controls, plotted as mean±SD for ICAM1, VCAM1, and TLR4 from 6 independent experiments. All experimental data were processed by GraphPad Prism 6.01 software that was used to plot histograms and calculate statistical significance by 2-way ANOVA. * P
    Figure Legend Snippet: rIFI16 synergizes with lipopolysaccharide (LPS) to increase proinflammatory cytokine expression through MyD88-dependent pathways. (A) HUVEC were grown in VEGF-depleted EGM-2 and stimulated for 24 h with either mock control, rIFI16 (50 μg/mL), LPS (10 ng/mL), or rIFI16+LPS mixture. Each RT-PCR reaction was performed with Bio-Rad CFX96 and relative normalized expression of mRNA was calculated using comparative Ct method. (B) MyD88/TLR4 silencing/neutralization inhibits rIFI16 cytokine stimulating activity and LPS synergy. HUVEC were stimulated for 24 h with mock control, rIFI16 (50 μg/mL), LPS (10 ng/mL), and rIFI16+LPS in the presence of no siRNA/antibodies (Control dataset), siRNA control, siRNA MyD88, Control IgG, or anti-TLR antibodies. (C) Flow cytometry histograms showing expression of ICAM1, VCAM1, and TLR4 in HUVEC when stimulated with mock control, rIFI16 (50 μg/mL), or LPS (10 ng/mL). One representative example from 6 independent experiments is shown. The shaded histograms represent binding of anti-ICAM1, anti-VCAM1, and anti-TLR4 antibodies and open histograms represent corresponding untreated controls. Isotype controls for each antibody were used in parallel to identify unspecific staining. (D) Histograms representing% positive cells after treatment and normalized mean fluorescence intensity as compared to isotype controls, plotted as mean±SD for ICAM1, VCAM1, and TLR4 from 6 independent experiments. All experimental data were processed by GraphPad Prism 6.01 software that was used to plot histograms and calculate statistical significance by 2-way ANOVA. * P

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Neutralization, Activity Assay, Flow Cytometry, Cytometry, Binding Assay, Staining, Fluorescence, Software

    5) Product Images from "Simultaneous detection of duck circovirus and novel goose parvovirus via SYBR green I-based duplex real-time polymerase chain reaction analysis"

    Article Title: Simultaneous detection of duck circovirus and novel goose parvovirus via SYBR green I-based duplex real-time polymerase chain reaction analysis

    Journal: Molecular and Cellular Probes

    doi: 10.1016/j.mcp.2020.101648

    Standard SYBR Green I-based quantitative PCR curves generated by plotting the mean Cq values from triplicate samples versus the concentrations of DuCV plasmid DNA standards, which were serially diluted 10-fold over concentrations ranging from 1 × 10 10 to 1 × 10 3 copies/μL. The coefficient of determination (R 2 ) and the line equation of the regression curve (y) were calculated using a CFX96™ Real-Time PCR Detection System. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Standard SYBR Green I-based quantitative PCR curves generated by plotting the mean Cq values from triplicate samples versus the concentrations of DuCV plasmid DNA standards, which were serially diluted 10-fold over concentrations ranging from 1 × 10 10 to 1 × 10 3 copies/μL. The coefficient of determination (R 2 ) and the line equation of the regression curve (y) were calculated using a CFX96™ Real-Time PCR Detection System. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: SYBR Green Assay, Real-time Polymerase Chain Reaction, Generated, Plasmid Preparation

    Standard SYBR Green I-based quantitative PCR curves generated by plotting the mean Cq values from triplicate samples versus the concentrations of NGPV plasmid DNA standards, which were serially diluted 10-fold over concentrations ranging from 1 × 10 10 to 1 × 10 3 copies/μL. The coefficient of determination (R 2 ) and the line equation of the regression curve (y) were calculated using a CFX96™ Real-Time PCR Detection System. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Standard SYBR Green I-based quantitative PCR curves generated by plotting the mean Cq values from triplicate samples versus the concentrations of NGPV plasmid DNA standards, which were serially diluted 10-fold over concentrations ranging from 1 × 10 10 to 1 × 10 3 copies/μL. The coefficient of determination (R 2 ) and the line equation of the regression curve (y) were calculated using a CFX96™ Real-Time PCR Detection System. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: SYBR Green Assay, Real-time Polymerase Chain Reaction, Generated, Plasmid Preparation

    6) Product Images from "Identification, gene expression and genetic polymorphism of zinc finger A20/AN1 stress-associated genes, HvSAP, in salt stressed barley from Kazakhstan"

    Article Title: Identification, gene expression and genetic polymorphism of zinc finger A20/AN1 stress-associated genes, HvSAP, in salt stressed barley from Kazakhstan

    Journal: BMC Plant Biology

    doi: 10.1186/s12870-020-02332-4

    Allele discrimination for the Amplifluor-like SNP marker KATU-B30. Fifty F 3 breeding lines from the segregating population Granal × Baisheshek were used. Parental genotypes are designated as: ♀ P 1 , Granal, and ♂ P 2 , Baisheshek. Relative Fluorescence Units (RFU) for fluorophores FAM and HEX were transformed automatically into genotyping of alleles 1 and 2, respectively, using a BioRad CFX96 Real-Time PCR Detection System Instrument
    Figure Legend Snippet: Allele discrimination for the Amplifluor-like SNP marker KATU-B30. Fifty F 3 breeding lines from the segregating population Granal × Baisheshek were used. Parental genotypes are designated as: ♀ P 1 , Granal, and ♂ P 2 , Baisheshek. Relative Fluorescence Units (RFU) for fluorophores FAM and HEX were transformed automatically into genotyping of alleles 1 and 2, respectively, using a BioRad CFX96 Real-Time PCR Detection System Instrument

    Techniques Used: Marker, Fluorescence, Transformation Assay, Real-time Polymerase Chain Reaction

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Performance Evaluation of the Real-Q Cytomegalovirus (CMV) Quantification Kit Using Two Real-Time PCR Systems for Quantifying CMV DNA in Whole Blood
    Article Snippet: .. In the present study, we assessed the performance of the Real-Q assay for quantifying CMV DNA in WB using two real-time PCR platforms: the 7500 Fast real-time PCR system (7500 Fast; Applied Biosystems, Foster City, CA, USA) and the CFX96 real-time PCR detection system (CFX96; Bio-Rad, Hercules, CA, USA). ..

    Article Title: A novel HRM assay for the simultaneous detection and differentiation of eight poxviruses of medical and veterinary importance
    Article Snippet: .. Furthermore, after performing the amplification steps using the classical PCR machine (Bio-Rad C1000) and transferring the plates containing the amplified product to the CFX96™ real-time PCR system (Bio-Rad) only for melting curve analysis, we have successfully assigned each virus to the correct species. ..

    Article Title: The Extracellular IFI16 Protein Propagates Inflammation in Endothelial Cells Via p38 MAPK and NF-κB p65 Activation
    Article Snippet: .. The quantitative real-time PCR analyses were performed using CFX96 Real-Time PCR Detection System (Bio-Rad) with SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad) and amplification conditions as instructed in the manufacturer's protocol, up to 40 cycles of PCR. ..

    Article Title: Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Sig
    Article Snippet: .. The fluorescent probe-based 2009 H1N1 NA signature-specific assay was conducted with the same cycling conditions and volumes described above for either the CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA) or the LightCycler 480 real-time PCR system, using the SuperScript III Platinum one-step quantitative RT-PCR kit (Invitrogen, Carlsbad, CA), with 200 nM each primer and 100 nM fluorescently labeled probe. ..

    Article Title: Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Sig
    Article Snippet: .. The SYBR green assays using the 2009 H1N1 NA signature primers were performed in two formats: a 96-well plate assay was done using a CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA) in a final volume of 25 μl, and a 384-well plate assay was performed in a LightCycler 480 real-time PCR system (Roche, Indianapolis, IN) in a 10-μl final reaction volume. .. Both assays were conducted using the SuperScript III Platinum SYBR green one-step qRT-PCR kit (Invitrogen, Carlsbad, CA), 1 to 2 μl of viral RNA, 200 nM each primer, and the following cycle conditions: an RT step at 55°C for 3 min, 95°C for 5 min, 40 cycles of 95°C for 15 s, 60°C for 30 s, and 40°C for 1 min, followed by a melting curve to confirm product specificity.

    Article Title: BCL-XL expression is essential for human erythropoiesis and engraftment of hematopoietic stem cells
    Article Snippet: .. Real time Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed by using BIO-RAD (CFX96 Touch) real time PCR detection system and SYBR Green master mix (Thermofisher). .. Expression of gene of interest was normalized to either 18S or 36B4 (Supplementary Table ).

    Amplification:

    Article Title: A novel HRM assay for the simultaneous detection and differentiation of eight poxviruses of medical and veterinary importance
    Article Snippet: .. Furthermore, after performing the amplification steps using the classical PCR machine (Bio-Rad C1000) and transferring the plates containing the amplified product to the CFX96™ real-time PCR system (Bio-Rad) only for melting curve analysis, we have successfully assigned each virus to the correct species. ..

    Article Title: The Extracellular IFI16 Protein Propagates Inflammation in Endothelial Cells Via p38 MAPK and NF-κB p65 Activation
    Article Snippet: .. The quantitative real-time PCR analyses were performed using CFX96 Real-Time PCR Detection System (Bio-Rad) with SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad) and amplification conditions as instructed in the manufacturer's protocol, up to 40 cycles of PCR. ..

    Transferring:

    Article Title: A novel HRM assay for the simultaneous detection and differentiation of eight poxviruses of medical and veterinary importance
    Article Snippet: .. Furthermore, after performing the amplification steps using the classical PCR machine (Bio-Rad C1000) and transferring the plates containing the amplified product to the CFX96™ real-time PCR system (Bio-Rad) only for melting curve analysis, we have successfully assigned each virus to the correct species. ..

    Quantitative RT-PCR:

    Article Title: Effects of Long-Term Supplementation of Blue-Green Algae on Lipid Metabolism in C57BL/6J mice
    Article Snippet: .. qRT-PCR analysis for gene expression was conducted as previously described using the SYBR Green procedure and CFX96™ realtime PCR detection system (BioRad, Hercules, CA) [ , ]. .. Primer sequences were designed according to GenBank database using the Beacon Designer software (Premier Biosoft, Palo Alto, CA) and the list of primer sequences is provided in .

    Article Title: Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Sig
    Article Snippet: .. The fluorescent probe-based 2009 H1N1 NA signature-specific assay was conducted with the same cycling conditions and volumes described above for either the CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA) or the LightCycler 480 real-time PCR system, using the SuperScript III Platinum one-step quantitative RT-PCR kit (Invitrogen, Carlsbad, CA), with 200 nM each primer and 100 nM fluorescently labeled probe. ..

    Article Title: BCL-XL expression is essential for human erythropoiesis and engraftment of hematopoietic stem cells
    Article Snippet: .. Real time Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed by using BIO-RAD (CFX96 Touch) real time PCR detection system and SYBR Green master mix (Thermofisher). .. Expression of gene of interest was normalized to either 18S or 36B4 (Supplementary Table ).

    SYBR Green Assay:

    Article Title: Effects of Long-Term Supplementation of Blue-Green Algae on Lipid Metabolism in C57BL/6J mice
    Article Snippet: .. qRT-PCR analysis for gene expression was conducted as previously described using the SYBR Green procedure and CFX96™ realtime PCR detection system (BioRad, Hercules, CA) [ , ]. .. Primer sequences were designed according to GenBank database using the Beacon Designer software (Premier Biosoft, Palo Alto, CA) and the list of primer sequences is provided in .

    Article Title: The Extracellular IFI16 Protein Propagates Inflammation in Endothelial Cells Via p38 MAPK and NF-κB p65 Activation
    Article Snippet: .. The quantitative real-time PCR analyses were performed using CFX96 Real-Time PCR Detection System (Bio-Rad) with SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad) and amplification conditions as instructed in the manufacturer's protocol, up to 40 cycles of PCR. ..

    Article Title: Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Sig
    Article Snippet: .. The SYBR green assays using the 2009 H1N1 NA signature primers were performed in two formats: a 96-well plate assay was done using a CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA) in a final volume of 25 μl, and a 384-well plate assay was performed in a LightCycler 480 real-time PCR system (Roche, Indianapolis, IN) in a 10-μl final reaction volume. .. Both assays were conducted using the SuperScript III Platinum SYBR green one-step qRT-PCR kit (Invitrogen, Carlsbad, CA), 1 to 2 μl of viral RNA, 200 nM each primer, and the following cycle conditions: an RT step at 55°C for 3 min, 95°C for 5 min, 40 cycles of 95°C for 15 s, 60°C for 30 s, and 40°C for 1 min, followed by a melting curve to confirm product specificity.

    Article Title: BCL-XL expression is essential for human erythropoiesis and engraftment of hematopoietic stem cells
    Article Snippet: .. Real time Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed by using BIO-RAD (CFX96 Touch) real time PCR detection system and SYBR Green master mix (Thermofisher). .. Expression of gene of interest was normalized to either 18S or 36B4 (Supplementary Table ).

    Polymerase Chain Reaction:

    Article Title: Effects of Long-Term Supplementation of Blue-Green Algae on Lipid Metabolism in C57BL/6J mice
    Article Snippet: .. qRT-PCR analysis for gene expression was conducted as previously described using the SYBR Green procedure and CFX96™ realtime PCR detection system (BioRad, Hercules, CA) [ , ]. .. Primer sequences were designed according to GenBank database using the Beacon Designer software (Premier Biosoft, Palo Alto, CA) and the list of primer sequences is provided in .

    Article Title: A novel HRM assay for the simultaneous detection and differentiation of eight poxviruses of medical and veterinary importance
    Article Snippet: .. Furthermore, after performing the amplification steps using the classical PCR machine (Bio-Rad C1000) and transferring the plates containing the amplified product to the CFX96™ real-time PCR system (Bio-Rad) only for melting curve analysis, we have successfully assigned each virus to the correct species. ..

    Article Title: The Extracellular IFI16 Protein Propagates Inflammation in Endothelial Cells Via p38 MAPK and NF-κB p65 Activation
    Article Snippet: .. The quantitative real-time PCR analyses were performed using CFX96 Real-Time PCR Detection System (Bio-Rad) with SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad) and amplification conditions as instructed in the manufacturer's protocol, up to 40 cycles of PCR. ..

    Article Title: Exploration of Using Antisense Peptide Nucleic Acid (PNA)-cell Penetrating Peptide (CPP) as a Novel Bactericide against Fire Blight Pathogen Erwinia amylovora
    Article Snippet: .. The reaction was performed on a Biorad CFX96 Realtime PCR machine. ..

    Labeling:

    Article Title: Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Sig
    Article Snippet: .. The fluorescent probe-based 2009 H1N1 NA signature-specific assay was conducted with the same cycling conditions and volumes described above for either the CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA) or the LightCycler 480 real-time PCR system, using the SuperScript III Platinum one-step quantitative RT-PCR kit (Invitrogen, Carlsbad, CA), with 200 nM each primer and 100 nM fluorescently labeled probe. ..

    Expressing:

    Article Title: Effects of Long-Term Supplementation of Blue-Green Algae on Lipid Metabolism in C57BL/6J mice
    Article Snippet: .. qRT-PCR analysis for gene expression was conducted as previously described using the SYBR Green procedure and CFX96™ realtime PCR detection system (BioRad, Hercules, CA) [ , ]. .. Primer sequences were designed according to GenBank database using the Beacon Designer software (Premier Biosoft, Palo Alto, CA) and the list of primer sequences is provided in .

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: BCL-XL expression is essential for human erythropoiesis and engraftment of hematopoietic stem cells
    Article Snippet: .. Real time Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed by using BIO-RAD (CFX96 Touch) real time PCR detection system and SYBR Green master mix (Thermofisher). .. Expression of gene of interest was normalized to either 18S or 36B4 (Supplementary Table ).

    Western Blot:

    Article Title: Performance Evaluation of the Real-Q Cytomegalovirus (CMV) Quantification Kit Using Two Real-Time PCR Systems for Quantifying CMV DNA in Whole Blood
    Article Snippet: .. In the present study, we assessed the performance of the Real-Q assay for quantifying CMV DNA in WB using two real-time PCR platforms: the 7500 Fast real-time PCR system (7500 Fast; Applied Biosystems, Foster City, CA, USA) and the CFX96 real-time PCR detection system (CFX96; Bio-Rad, Hercules, CA, USA). ..

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    <t>qRT-PCR</t> analysis of relative gene expression of human periosteum cells exposed to PASF scaffolds. Genes <t>(Col(Ⅰ)-α1/Col</t> (Ⅰ)-α2), Runx2, ATF4, ALP and OCN were assayed over a 16 day period after human periosteum cells were cultured on the respective scaffolds. (ANOVA with * indicating statistical significance for p
    Real Time Pcr Machine, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1485 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    real time pcr machine - by Bioz Stars, 2021-01
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    Modular thermal cycler platform includes C1000 Touch Thermal Cycler chassis CFX96 Deep Well Optical Reaction Module cables To be used with CFX Manager Software Industrial Diagnostic Edition
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    Modular thermal cycler platform includes C1000 Touch Thermal Cycler Chassis CFX96 Optical Reaction Module CFX Maestro Software qbase Software license cables reagents consumables
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    qRT-PCR analysis of relative gene expression of human periosteum cells exposed to PASF scaffolds. Genes (Col(Ⅰ)-α1/Col (Ⅰ)-α2), Runx2, ATF4, ALP and OCN were assayed over a 16 day period after human periosteum cells were cultured on the respective scaffolds. (ANOVA with * indicating statistical significance for p

    Journal: Journal of biomedical materials research. Part A

    Article Title: Human Periosteum Cell Osteogenic Differentiation Enhanced by Ionic Silicon Release from Porous Amorphous Silica Fibrous Scaffolds

    doi: 10.1002/jbm.a.35412

    Figure Lengend Snippet: qRT-PCR analysis of relative gene expression of human periosteum cells exposed to PASF scaffolds. Genes (Col(Ⅰ)-α1/Col (Ⅰ)-α2), Runx2, ATF4, ALP and OCN were assayed over a 16 day period after human periosteum cells were cultured on the respective scaffolds. (ANOVA with * indicating statistical significance for p

    Article Snippet: The gene expression of GAPDH, osteocalcin (OCN) and core-bending factor (Cbfa1/Runx2), activating transcription factor 4 (ATF4), collagen type 1 (Col(Ⅰ)-α1, Col(Ⅰ)-α2) and alkaline phosphatase (ALP) was detected by a real-time PCR machine (CFX96, Bio-RAD Inc., Emeryville, CA) and using TaqMan assay kits (Life Technologies; Carlsbad, CA).

    Techniques: Quantitative RT-PCR, Expressing, ALP Assay, Cell Culture

    Highly specific NA signature primers for the 2009 H1N1 pandemic influenza virus. (A) Amplification of pandemic 2009 H1N1 NA in a conventional RT-PCR assay. RNA extracted from 1 × 10 7 to 1 × 10 8 PFU/ml of the shown H1N1 or H3N2 influenza viruses was subjected to a one-step RT-PCR using either the 2009 H1N1 NA signature primers (upper panel) or M segment consensus primers (lower panel) and run on a 1.2% agarose gel for detection by ethidium bromide. (B) Performance of 2009 H1N1 NA signature primers in a SYBR green assay. Specific NA signal curves obtained in a SYBR green one-step RT-PCR assay for the reference strains are shown. (C) Melting curve confirmation of specific products. In panels B and C, each sample was run in triplicate in a 96-well plate (25-μl final volume) using a Bio-Rad CFX96 real-time PCR detection system. Data are representative of two independent experiments with identical results.

    Journal: Journal of Clinical Microbiology

    Article Title: Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿Development and Characterization of a Highly Specific and Sensitive SYBR Green Reverse Transcriptase PCR Assay for Detection of the 2009 Pandemic H1N1 Influenza Virus on the Basis of Sequence Signatures ▿ †

    doi: 10.1128/JCM.01142-10

    Figure Lengend Snippet: Highly specific NA signature primers for the 2009 H1N1 pandemic influenza virus. (A) Amplification of pandemic 2009 H1N1 NA in a conventional RT-PCR assay. RNA extracted from 1 × 10 7 to 1 × 10 8 PFU/ml of the shown H1N1 or H3N2 influenza viruses was subjected to a one-step RT-PCR using either the 2009 H1N1 NA signature primers (upper panel) or M segment consensus primers (lower panel) and run on a 1.2% agarose gel for detection by ethidium bromide. (B) Performance of 2009 H1N1 NA signature primers in a SYBR green assay. Specific NA signal curves obtained in a SYBR green one-step RT-PCR assay for the reference strains are shown. (C) Melting curve confirmation of specific products. In panels B and C, each sample was run in triplicate in a 96-well plate (25-μl final volume) using a Bio-Rad CFX96 real-time PCR detection system. Data are representative of two independent experiments with identical results.

    Article Snippet: The SYBR green assays using the 2009 H1N1 NA signature primers were performed in two formats: a 96-well plate assay was done using a CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA) in a final volume of 25 μl, and a 384-well plate assay was performed in a LightCycler 480 real-time PCR system (Roche, Indianapolis, IN) in a 10-μl final reaction volume.

    Techniques: Amplification, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, SYBR Green Assay, Real-time Polymerase Chain Reaction

    Bland-Altman plot comparing the artus cytomegalovirus (CMV) RG PCR kit and the Real-Q CMV Quantification kit on different real-time PCR platforms: (A) the 7500 Fast Real-time PCR system, (B) the CFX96 real-time PCR detection system. Solid lines are the mean differences between the values; dashed lines are the mean difference plus or minus 1.96 SD (95% confidential interval of mean difference).

    Journal: Annals of Laboratory Medicine

    Article Title: Performance Evaluation of the Real-Q Cytomegalovirus (CMV) Quantification Kit Using Two Real-Time PCR Systems for Quantifying CMV DNA in Whole Blood

    doi: 10.3343/alm.2016.36.6.603

    Figure Lengend Snippet: Bland-Altman plot comparing the artus cytomegalovirus (CMV) RG PCR kit and the Real-Q CMV Quantification kit on different real-time PCR platforms: (A) the 7500 Fast Real-time PCR system, (B) the CFX96 real-time PCR detection system. Solid lines are the mean differences between the values; dashed lines are the mean difference plus or minus 1.96 SD (95% confidential interval of mean difference).

    Article Snippet: In the present study, we assessed the performance of the Real-Q assay for quantifying CMV DNA in WB using two real-time PCR platforms: the 7500 Fast real-time PCR system (7500 Fast; Applied Biosystems, Foster City, CA, USA) and the CFX96 real-time PCR detection system (CFX96; Bio-Rad, Hercules, CA, USA).

    Techniques: Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    Linearity of the Real-Q cytomegalovirus (CMV) Quantification kit on different real-time PCR platforms: (A) the 7500 Fast real-time PCR system and (B) the CFX96 real-time PCR detection system.

    Journal: Annals of Laboratory Medicine

    Article Title: Performance Evaluation of the Real-Q Cytomegalovirus (CMV) Quantification Kit Using Two Real-Time PCR Systems for Quantifying CMV DNA in Whole Blood

    doi: 10.3343/alm.2016.36.6.603

    Figure Lengend Snippet: Linearity of the Real-Q cytomegalovirus (CMV) Quantification kit on different real-time PCR platforms: (A) the 7500 Fast real-time PCR system and (B) the CFX96 real-time PCR detection system.

    Article Snippet: In the present study, we assessed the performance of the Real-Q assay for quantifying CMV DNA in WB using two real-time PCR platforms: the 7500 Fast real-time PCR system (7500 Fast; Applied Biosystems, Foster City, CA, USA) and the CFX96 real-time PCR detection system (CFX96; Bio-Rad, Hercules, CA, USA).

    Techniques: Real-time Polymerase Chain Reaction

    Amount of E. amylovora Ea110 cells on detached apple stigmas after treatment with anti- acpP -CPP1 and streptomycin. E. amylovora Ea110 cells (10 7 CFU/ml) were inoculated on the stigma of freshly opened ‘McIntosh’ flowers (1 μl per flower by a pipette). Twenty microliters of anti- acpP -CPP1 (100, 20, and 4 μM) were evenly applied onto the stigmas of each flower 2 h before and 19 h after the inoculation. Water and streptomycin (100 μM) were used as negative and positive controls. In the PNA–bacteria mix treatment, anti- acpP -CPP1 (20 μM) was mixed with an equal volume of E. amylovora cells (10 7 CFU/ml) in a microcentrifuge tube and incubated for 30 min at room temperature (22–25°C). Two microliters of the mixture were applied evenly onto the five stigmas of the flowers. All flowers were incubated at room temperature and the pathogen population on the apple stigmas was quantified at 42 h post inoculation, using a Taqman probe realtime PCR assay. Statistical analysis was performed using ANOVA with α = 0.05. The level of significance (denoted by different letters) was calculated using LSD (α = 0.05).

    Journal: Frontiers in Microbiology

    Article Title: Exploration of Using Antisense Peptide Nucleic Acid (PNA)-cell Penetrating Peptide (CPP) as a Novel Bactericide against Fire Blight Pathogen Erwinia amylovora

    doi: 10.3389/fmicb.2017.00687

    Figure Lengend Snippet: Amount of E. amylovora Ea110 cells on detached apple stigmas after treatment with anti- acpP -CPP1 and streptomycin. E. amylovora Ea110 cells (10 7 CFU/ml) were inoculated on the stigma of freshly opened ‘McIntosh’ flowers (1 μl per flower by a pipette). Twenty microliters of anti- acpP -CPP1 (100, 20, and 4 μM) were evenly applied onto the stigmas of each flower 2 h before and 19 h after the inoculation. Water and streptomycin (100 μM) were used as negative and positive controls. In the PNA–bacteria mix treatment, anti- acpP -CPP1 (20 μM) was mixed with an equal volume of E. amylovora cells (10 7 CFU/ml) in a microcentrifuge tube and incubated for 30 min at room temperature (22–25°C). Two microliters of the mixture were applied evenly onto the five stigmas of the flowers. All flowers were incubated at room temperature and the pathogen population on the apple stigmas was quantified at 42 h post inoculation, using a Taqman probe realtime PCR assay. Statistical analysis was performed using ANOVA with α = 0.05. The level of significance (denoted by different letters) was calculated using LSD (α = 0.05).

    Article Snippet: The reaction was performed on a Biorad CFX96 Realtime PCR machine.

    Techniques: Transferring, Incubation, Polymerase Chain Reaction