centrifugal filter device  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Amicon Ultra 15
    Description:
    High recovery centrifugal filters for concentrating samples Can be used to concentrate biological samples containing antigens antibodies enzymes nucleic acids or micoorganisms Excellen for use to purify macromolecular components from cell lysates Ideal for desalting and buffer exchanges Swing bucket rotorL 4 000 x g Fixed angle rotor 35 5 000 x g High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut offs High retentate recovery of 90 Vertical membrane reduces concentration polarization for ultra fast spin times as fast as 10 15 mins Heat sealed membrane minimizes downstream extractables 100 integrity tested for reliable performance Convenient sample monitoring with translucent housing and volume graduations Direct pipettor sample access eliminates processing step to recover concentrate High concentration factors of 80 100X
    Catalog Number:
    z740211
    Price:
    None
    Buy from Supplier


    Structured Review

    Millipore centrifugal filter device
    Amicon Ultra 15
    High recovery centrifugal filters for concentrating samples Can be used to concentrate biological samples containing antigens antibodies enzymes nucleic acids or micoorganisms Excellen for use to purify macromolecular components from cell lysates Ideal for desalting and buffer exchanges Swing bucket rotorL 4 000 x g Fixed angle rotor 35 5 000 x g High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut offs High retentate recovery of 90 Vertical membrane reduces concentration polarization for ultra fast spin times as fast as 10 15 mins Heat sealed membrane minimizes downstream extractables 100 integrity tested for reliable performance Convenient sample monitoring with translucent housing and volume graduations Direct pipettor sample access eliminates processing step to recover concentrate High concentration factors of 80 100X
    https://www.bioz.com/result/centrifugal filter device/product/Millipore
    Average 99 stars, based on 200 article reviews
    Price from $9.99 to $1999.99
    centrifugal filter device - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Flow Cytometry:

    Article Title: PRMT1-mediated methylation of MICU1 determines the UCP2/3 dependency of mitochondrial Ca2+ uptake in immortalized cells
    Article Snippet: .. The flow through was further concentrated to a final volume of 2 ml using 10 kD cutoff Amicon Ultra-15 ml centrifugal filter units (Millipore) in a centrifuge with Swinging-bucket rotor. .. A size-exclusion step was performed to remove contaminants using a Superdex 75 16/600 column attached to an Äkta pure FPLC system and equilibrated in MICU1 ITC buffer (20 mM Tris pH 7.0, 500 mM NaCl, 50 mM Arg and 2 mM BME).

    Filtration:

    Article Title: Solution NMR and calorimetric analysis of Rem2 binding to the Ca2+ channel β4 subunit: a low affinity interaction is required for inhibition of Cav2.1 Ca2+ currents
    Article Snippet: .. Protein purity was analyzed on SDS-PAGE gels, and the protein samples were concentrated via centrifugal filtration using 10 kDa exclusion filters for Rem2, SH3, and GK and a 30 kDa exclusion filter for the SH3-GK complex (Amicon Ultra 15; EMD, Millipore). ..

    Purification:

    Article Title: Nucleocapsid protein-dependent assembly of the RNA packaging signal of Middle East respiratory syndrome coronavirus
    Article Snippet: .. The purified proteins were concentrated with Amicon Ultra-15 concentrator (Millipore) and kept in 50% glycerol at − 80 °C for filter binding assay. ..

    Article Title: Expression of mouse beta defensin 2 in escherichia coli and its broad-spectrum antimicrobial activity
    Article Snippet: .. The purified fusion protein (TrxA-mBD2) was desalted by Amicon® Ultra-15 10K Centrifugal Filter Devices (Millipore, USA) and subjected to recombinant Enterokinase-His (rEK-His, Zhongda Nanhai Marine Biotech, Guangdong, China) digestion at 25°C for 16 h in recommended buffer (0.2 mM Tris-HCl, 100 mM NaCl, pH 8.0). .. The mixture buffer was further purified by His Trap™ HP crude.

    Filter-binding Assay:

    Article Title: Nucleocapsid protein-dependent assembly of the RNA packaging signal of Middle East respiratory syndrome coronavirus
    Article Snippet: .. The purified proteins were concentrated with Amicon Ultra-15 concentrator (Millipore) and kept in 50% glycerol at − 80 °C for filter binding assay. ..

    Recombinant:

    Article Title: Expression of mouse beta defensin 2 in escherichia coli and its broad-spectrum antimicrobial activity
    Article Snippet: .. The purified fusion protein (TrxA-mBD2) was desalted by Amicon® Ultra-15 10K Centrifugal Filter Devices (Millipore, USA) and subjected to recombinant Enterokinase-His (rEK-His, Zhongda Nanhai Marine Biotech, Guangdong, China) digestion at 25°C for 16 h in recommended buffer (0.2 mM Tris-HCl, 100 mM NaCl, pH 8.0). .. The mixture buffer was further purified by His Trap™ HP crude.

    SDS Page:

    Article Title: Solution NMR and calorimetric analysis of Rem2 binding to the Ca2+ channel β4 subunit: a low affinity interaction is required for inhibition of Cav2.1 Ca2+ currents
    Article Snippet: .. Protein purity was analyzed on SDS-PAGE gels, and the protein samples were concentrated via centrifugal filtration using 10 kDa exclusion filters for Rem2, SH3, and GK and a 30 kDa exclusion filter for the SH3-GK complex (Amicon Ultra 15; EMD, Millipore). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore ultrafiltered
    Results of ion selective electrode measurements from (a) lumber blocks and (b) sawdust treatments using MCA high treated lumber. In addition, results of periodic sampling for ICP-AES analysis on unfiltered (blocks), 0.45 μm filtered (sawdust), and 3 kDa <t>ultrafiltered</t> samples are presented. Electrode data are culled to report an hourly measurement.
    Ultrafiltered, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrafiltered/product/Millipore
    Average 99 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    ultrafiltered - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    85
    Millipore microcon centrifugal filtering device
    Evaluation of pore formation within cross-linked polymersomes following surfactant solubilization. The water-soluble, low-molecular-weight dye carboxyfluorescein was encapsulated into chemically cross-linked polymersomes with and without 15% phospholipid. The polymerized vesicles were treated with an equivalent volume of buffer or Triton X-100 (final concentration = 10 mM) and then centrifuged on a <t>Microcon</t> centrifugal filtering device with a 100 kDa MWCO membrane (Millipore). The liquid that flowed through the filter was tested for fluorescence. (a) Cross-linked polymersomes doped with 15% phospholipid. (b) Cross-linked polymersomes without phospholipids.
    Microcon Centrifugal Filtering Device, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcon centrifugal filtering device/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microcon centrifugal filtering device - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    99
    Millipore microcon centrifugal filter devices
    Centrifugal filter device assay. Biofilms were cultured in the sample reservoirs of <t>Microcon</t> centrifugal filter devices as described in Materials and Methods. The sample reservoir is positioned on top of a filter with a 100-kDa cutoff. Inocula were transferred
    Microcon Centrifugal Filter Devices, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcon centrifugal filter devices/product/Millipore
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    microcon centrifugal filter devices - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Results of ion selective electrode measurements from (a) lumber blocks and (b) sawdust treatments using MCA high treated lumber. In addition, results of periodic sampling for ICP-AES analysis on unfiltered (blocks), 0.45 μm filtered (sawdust), and 3 kDa ultrafiltered samples are presented. Electrode data are culled to report an hourly measurement.

    Journal: Environmental toxicology and chemistry

    Article Title: Assessing the Release of Copper from Nanocopper-treated and Conventional Copper-treated Lumber into Marine Waters: (2) Forms and Bioavailability

    doi: 10.1002/etc.4140

    Figure Lengend Snippet: Results of ion selective electrode measurements from (a) lumber blocks and (b) sawdust treatments using MCA high treated lumber. In addition, results of periodic sampling for ICP-AES analysis on unfiltered (blocks), 0.45 μm filtered (sawdust), and 3 kDa ultrafiltered samples are presented. Electrode data are culled to report an hourly measurement.

    Article Snippet: In addition, at time points 1, 7, and 28 da, an additional ultrafiltered (3 kDa Amicon Ultra-15 centrifugal filter device, EMD Millipore) water sample was taken to quantify the presence of dissolved copper.

    Techniques: Sampling

    Evaluation of pore formation within cross-linked polymersomes following surfactant solubilization. The water-soluble, low-molecular-weight dye carboxyfluorescein was encapsulated into chemically cross-linked polymersomes with and without 15% phospholipid. The polymerized vesicles were treated with an equivalent volume of buffer or Triton X-100 (final concentration = 10 mM) and then centrifuged on a Microcon centrifugal filtering device with a 100 kDa MWCO membrane (Millipore). The liquid that flowed through the filter was tested for fluorescence. (a) Cross-linked polymersomes doped with 15% phospholipid. (b) Cross-linked polymersomes without phospholipids.

    Journal: Langmuir

    Article Title: Paramagnetic Porous Polymersomes

    doi: 10.1021/la801027q

    Figure Lengend Snippet: Evaluation of pore formation within cross-linked polymersomes following surfactant solubilization. The water-soluble, low-molecular-weight dye carboxyfluorescein was encapsulated into chemically cross-linked polymersomes with and without 15% phospholipid. The polymerized vesicles were treated with an equivalent volume of buffer or Triton X-100 (final concentration = 10 mM) and then centrifuged on a Microcon centrifugal filtering device with a 100 kDa MWCO membrane (Millipore). The liquid that flowed through the filter was tested for fluorescence. (a) Cross-linked polymersomes doped with 15% phospholipid. (b) Cross-linked polymersomes without phospholipids.

    Article Snippet: Following the extraction of the phospholipids with Triton X-100, the polymersomes were centrifuged on a Microcon centrifugal filtering device (Millipore) with a molecular weight cutoff of 100 kDa.

    Techniques: Molecular Weight, Concentration Assay, Fluorescence

    Retention of generation-2 dendrimer within cross-linked porous polymersomes. TRITC-labeled dendrimer was encapsulated into chemically cross-linked polymersomes containing 15% phospholipid. The polymerized vesicles were treated with an equivalent volume of buffer or Triton X-100 (final concentration = 10 mM) and then centrifuged on a Microcon centrifugal filtering device with a 100 kDa MWCO membrane (Millipore). The liquid that flowed through the filter was tested for fluorescence. The emission spectrum of vesicles treated with Triton X-100 was nearly identical to that of the vesicles treated by buffer, indicating that the dendrimer is retained within the porous vesicles.

    Journal: Langmuir

    Article Title: Paramagnetic Porous Polymersomes

    doi: 10.1021/la801027q

    Figure Lengend Snippet: Retention of generation-2 dendrimer within cross-linked porous polymersomes. TRITC-labeled dendrimer was encapsulated into chemically cross-linked polymersomes containing 15% phospholipid. The polymerized vesicles were treated with an equivalent volume of buffer or Triton X-100 (final concentration = 10 mM) and then centrifuged on a Microcon centrifugal filtering device with a 100 kDa MWCO membrane (Millipore). The liquid that flowed through the filter was tested for fluorescence. The emission spectrum of vesicles treated with Triton X-100 was nearly identical to that of the vesicles treated by buffer, indicating that the dendrimer is retained within the porous vesicles.

    Article Snippet: Following the extraction of the phospholipids with Triton X-100, the polymersomes were centrifuged on a Microcon centrifugal filtering device (Millipore) with a molecular weight cutoff of 100 kDa.

    Techniques: Labeling, Concentration Assay, Fluorescence

    Centrifugal filter device assay. Biofilms were cultured in the sample reservoirs of Microcon centrifugal filter devices as described in Materials and Methods. The sample reservoir is positioned on top of a filter with a 100-kDa cutoff. Inocula were transferred

    Journal:

    Article Title: Poly-N-Acetylglucosamine Matrix Polysaccharide Impedes Fluid Convection and Transport of the Cationic Surfactant Cetylpyridinium Chloride through Bacterial Biofilms ▿

    doi: 10.1128/AEM.01900-08

    Figure Lengend Snippet: Centrifugal filter device assay. Biofilms were cultured in the sample reservoirs of Microcon centrifugal filter devices as described in Materials and Methods. The sample reservoir is positioned on top of a filter with a 100-kDa cutoff. Inocula were transferred

    Article Snippet: Biofilms were cultured in Microcon centrifugal filter devices (catalog no. YM-100; Millipore).

    Techniques: Cell Culture