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Promega celltiter glo one solution assay
Celltiter Glo One Solution Assay, supplied by Promega, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/celltiter glo one solution assay/product/Promega
Average 96 stars, based on 2 article reviews
Price from $9.99 to $1999.99
celltiter glo one solution assay - by Bioz Stars, 2020-04
96/100 stars

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Negative Control:

Article Title: Innate and adaptive immunity associated with resolution of acute woodchuck hepatitis virus infection in adult woodchucks
Article Snippet: PBMCs were stimulated with 0.02% dimethyl sulfoxide (DMSO, Sigma, unstimulated negative control), 0.5 μg/mL lipopolysaccharide (LPS, Sigma, positive control), and pools of peptides covering the entire WHcAg or WHsAg (Thermo Fisher Scientific). .. After 5 days of stimulation, cell proliferation was determined with the CellTiter Glo One Solution assay (Promega, Madison, WI).

Positive Control:

Article Title: Innate and adaptive immunity associated with resolution of acute woodchuck hepatitis virus infection in adult woodchucks
Article Snippet: PBMCs were stimulated with 0.02% dimethyl sulfoxide (DMSO, Sigma, unstimulated negative control), 0.5 μg/mL lipopolysaccharide (LPS, Sigma, positive control), and pools of peptides covering the entire WHcAg or WHsAg (Thermo Fisher Scientific). .. After 5 days of stimulation, cell proliferation was determined with the CellTiter Glo One Solution assay (Promega, Madison, WI).

Isolation:

Article Title: Innate and adaptive immunity associated with resolution of acute woodchuck hepatitis virus infection in adult woodchucks
Article Snippet: WHV-specific T-cell response Peripheral blood mononuclear cells (PBMCs) were isolated using the Ficoll-Paque density gradient centrifugation method and cultured in complete AIM-V medium (Thermo Fisher Scientific, Waltham, MA) in 96-well opaque plates (Sigma, St. Louis, MO) as described previously [ ]. .. After 5 days of stimulation, cell proliferation was determined with the CellTiter Glo One Solution assay (Promega, Madison, WI).

Infection:

Article Title: The Glucocorticoid Receptor (GR) Stimulates Herpes Simplex Virus 1 Productive Infection, in Part Because the Infected Cell Protein 0 (ICP0) Promoter Is Cooperatively Transactivated by the GR and Krüppel-Like Transcription Factor 15
Article Snippet: Paragraph title: Inhibition of productive infection by glucocorticoid receptor. ... Viability was assessed using the CellTiter-Glo luminescent cell viability assay (G8461; Promega, Madison, WI) according to the manufacturer’s instructions.

Concentration Assay:

Article Title: Innate and adaptive immunity associated with resolution of acute woodchuck hepatitis virus infection in adult woodchucks
Article Snippet: Peptides were dissolved for obtaining a final concentration of 10.0 μg/mL for each peptide in 0.02% DMSO. .. After 5 days of stimulation, cell proliferation was determined with the CellTiter Glo One Solution assay (Promega, Madison, WI).

Cell Culture:

Article Title: Innate and adaptive immunity associated with resolution of acute woodchuck hepatitis virus infection in adult woodchucks
Article Snippet: WHV-specific T-cell response Peripheral blood mononuclear cells (PBMCs) were isolated using the Ficoll-Paque density gradient centrifugation method and cultured in complete AIM-V medium (Thermo Fisher Scientific, Waltham, MA) in 96-well opaque plates (Sigma, St. Louis, MO) as described previously [ ]. .. After 5 days of stimulation, cell proliferation was determined with the CellTiter Glo One Solution assay (Promega, Madison, WI).

Staining:

Article Title: The Glucocorticoid Receptor (GR) Stimulates Herpes Simplex Virus 1 Productive Infection, in Part Because the Infected Cell Protein 0 (ICP0) Promoter Is Cooperatively Transactivated by the GR and Krüppel-Like Transcription Factor 15
Article Snippet: Plates were monitored for plaque development and stained using crystal violet. .. Viability was assessed using the CellTiter-Glo luminescent cell viability assay (G8461; Promega, Madison, WI) according to the manufacturer’s instructions.

Article Title: The Glucocorticoid Receptor (GR) Stimulates Herpes Simplex Virus 1 Productive Infection, in Part Because the Infected Cell Protein 0 (ICP0) Promoter Is Cooperatively Transactivated by the GR and Krüppel-Like Transcription Factor 15
Article Snippet: Plates were monitored for plaque development and stained using crystal violet. .. Viability was assessed using the CellTiter-Glo luminescent cell viability assay (G8461; Promega, Madison, WI) according to the manufacturer’s instructions.

Cell Viability Assay:

Article Title: The Glucocorticoid Receptor (GR) Stimulates Herpes Simplex Virus 1 Productive Infection, in Part Because the Infected Cell Protein 0 (ICP0) Promoter Is Cooperatively Transactivated by the GR and Krüppel-Like Transcription Factor 15
Article Snippet: .. Viability was assessed using the CellTiter-Glo luminescent cell viability assay (G8461; Promega, Madison, WI) according to the manufacturer’s instructions. .. Plates were read using a SpectraMax M3 microplate reader (Molecular Devices, San Jose, CA).

Article Title: The Glucocorticoid Receptor (GR) Stimulates Herpes Simplex Virus 1 Productive Infection, in Part Because the Infected Cell Protein 0 (ICP0) Promoter Is Cooperatively Transactivated by the GR and Krüppel-Like Transcription Factor 15
Article Snippet: .. Viability was assessed using the CellTiter-Glo luminescent cell viability assay (G8461; Promega, Madison, WI) according to the manufacturer’s instructions. .. Plates were read using a SpectraMax M3 microplate reader (Molecular Devices, San Jose, CA).

Gradient Centrifugation:

Article Title: Innate and adaptive immunity associated with resolution of acute woodchuck hepatitis virus infection in adult woodchucks
Article Snippet: WHV-specific T-cell response Peripheral blood mononuclear cells (PBMCs) were isolated using the Ficoll-Paque density gradient centrifugation method and cultured in complete AIM-V medium (Thermo Fisher Scientific, Waltham, MA) in 96-well opaque plates (Sigma, St. Louis, MO) as described previously [ ]. .. After 5 days of stimulation, cell proliferation was determined with the CellTiter Glo One Solution assay (Promega, Madison, WI).

Inhibition:

Article Title: The Glucocorticoid Receptor (GR) Stimulates Herpes Simplex Virus 1 Productive Infection, in Part Because the Infected Cell Protein 0 (ICP0) Promoter Is Cooperatively Transactivated by the GR and Krüppel-Like Transcription Factor 15
Article Snippet: Paragraph title: Inhibition of productive infection by glucocorticoid receptor. ... Viability was assessed using the CellTiter-Glo luminescent cell viability assay (G8461; Promega, Madison, WI) according to the manufacturer’s instructions.

Derivative Assay:

Article Title: Innate and adaptive immunity associated with resolution of acute woodchuck hepatitis virus infection in adult woodchucks
Article Snippet: After 5 days of stimulation, cell proliferation was determined with the CellTiter Glo One Solution assay (Promega, Madison, WI). .. The derived luminescence signal of triplicate cell cultures was averaged and expressed as a fold-change by dividing the average signal in the presence of stimulator (WHcAg- or WHsAg-derived peptides) by that in the absence of stimulator (DMSO-containing medium) at a particular week.

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    Promega celltiter glo one solution cell proliferation assay
    Linc00659 knockdown conferred colon cancer cell drug sensitivity. a HCT116 cells with shLinc00659#1 knockdown were exposed to various concentration (0, 1.3, 2.5 and 5.0 μg/ml) of oxaliplatin(left panel), 5-FU(middle panel), and irinotecan(right panel) for 48 h, respectively. Cell viability was then examined using <t>CellTiter-Glo</t> One Solution cell proliferation assay. b and c After exposure to 5 μg/mL oxaliplatin, 5-FU, and irinotecan for 48 h, the cell cycle distribution was examined by an image flow cytometry assay, and the percentage of sub-G1 was quantified in HCT116 cells with shLinc00659#1 knockdown and in control cells. d and e Cells were stained with both propidium iodide and Annexin V; apoptotic cells were analyzed by an image flow assay, and the percentage of apoptotic cells was quantified. 5-FU, 5-fluorouracil
    Celltiter Glo One Solution Cell Proliferation Assay, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 2389 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/celltiter glo one solution cell proliferation assay/product/Promega
    Average 99 stars, based on 2389 article reviews
    Price from $9.99 to $1999.99
    celltiter glo one solution cell proliferation assay - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    Linc00659 knockdown conferred colon cancer cell drug sensitivity. a HCT116 cells with shLinc00659#1 knockdown were exposed to various concentration (0, 1.3, 2.5 and 5.0 μg/ml) of oxaliplatin(left panel), 5-FU(middle panel), and irinotecan(right panel) for 48 h, respectively. Cell viability was then examined using CellTiter-Glo One Solution cell proliferation assay. b and c After exposure to 5 μg/mL oxaliplatin, 5-FU, and irinotecan for 48 h, the cell cycle distribution was examined by an image flow cytometry assay, and the percentage of sub-G1 was quantified in HCT116 cells with shLinc00659#1 knockdown and in control cells. d and e Cells were stained with both propidium iodide and Annexin V; apoptotic cells were analyzed by an image flow assay, and the percentage of apoptotic cells was quantified. 5-FU, 5-fluorouracil

    Journal: Molecular Cancer

    Article Title: Linc00659, a long noncoding RNA, acts as novel oncogene in regulating cancer cell growth in colorectal cancer

    doi: 10.1186/s12943-018-0821-1

    Figure Lengend Snippet: Linc00659 knockdown conferred colon cancer cell drug sensitivity. a HCT116 cells with shLinc00659#1 knockdown were exposed to various concentration (0, 1.3, 2.5 and 5.0 μg/ml) of oxaliplatin(left panel), 5-FU(middle panel), and irinotecan(right panel) for 48 h, respectively. Cell viability was then examined using CellTiter-Glo One Solution cell proliferation assay. b and c After exposure to 5 μg/mL oxaliplatin, 5-FU, and irinotecan for 48 h, the cell cycle distribution was examined by an image flow cytometry assay, and the percentage of sub-G1 was quantified in HCT116 cells with shLinc00659#1 knockdown and in control cells. d and e Cells were stained with both propidium iodide and Annexin V; apoptotic cells were analyzed by an image flow assay, and the percentage of apoptotic cells was quantified. 5-FU, 5-fluorouracil

    Article Snippet: Cell viability was determined using a CellTiter-Glo One Solution cell proliferation assay (Promega Corporation, Madison, WI, USA), which was performed according to the manufacturer’s instructions.

    Techniques: Concentration Assay, Proliferation Assay, Flow Cytometry, Cytometry, Staining

    A) MDAMB-231 cells transfected with siRNA targeting either no mRNA (= control, CTL), βarr1 or βarr2 were treated with 100 μM CoCl2 or grown under 1% O2. Untreated cells grown under normal oxygen levels represent the normoxia condition. 24 hours later, the amount of ATP was determined using CellTiter-Glo reagent (Promega). Cell viability was calculated as percentage ATP present according to the manufacturer’s protocol. The data presented are mean ± SEM from three experiments. *** p

    Journal: Oncogene

    Article Title: ?-arrestin1 mediates metastatic growth of breast cancer cells by facilitating HIF-1-dependent VEGF expression

    doi: 10.1038/onc.2011.238

    Figure Lengend Snippet: A) MDAMB-231 cells transfected with siRNA targeting either no mRNA (= control, CTL), βarr1 or βarr2 were treated with 100 μM CoCl2 or grown under 1% O2. Untreated cells grown under normal oxygen levels represent the normoxia condition. 24 hours later, the amount of ATP was determined using CellTiter-Glo reagent (Promega). Cell viability was calculated as percentage ATP present according to the manufacturer’s protocol. The data presented are mean ± SEM from three experiments. *** p

    Article Snippet: β-arrestin1 expression is critical for the viability of breast carcinoma cells under hypoxic stress To discern whether expression of β-arrestins 1 and 2 affects cancer cell viability, we performed CellTiter-Glo® (Promega) luminescent cell viability assay on breast carcinoma cells transfected with siRNA targeting no mRNA (control), β-arrestin1 or β-arrestin2.

    Techniques: Transfection, CTL Assay

    ADH-1 induces cell death in vitro. ADH-1 (1 mg/ml) was added to exponentially growing cultures of epithelial cells, fibroblasts and the indicated NB cell lines. Cell survival was measured at 12, 24 and 48 h after addition of ADH-1, using the CellTiter-Glo luminescent Cell Viability assay (Promega). Data were normalized to no drug treatment. Data are mean ± standard deviation (n = 3).

    Journal: PLoS ONE

    Article Title: N-Cadherin in Neuroblastoma Disease: Expression and Clinical Significance

    doi: 10.1371/journal.pone.0031206

    Figure Lengend Snippet: ADH-1 induces cell death in vitro. ADH-1 (1 mg/ml) was added to exponentially growing cultures of epithelial cells, fibroblasts and the indicated NB cell lines. Cell survival was measured at 12, 24 and 48 h after addition of ADH-1, using the CellTiter-Glo luminescent Cell Viability assay (Promega). Data were normalized to no drug treatment. Data are mean ± standard deviation (n = 3).

    Article Snippet: The number of viable cells in culture was measured using the CellTiter-Glo luminescent Cell Viability assay (Promega) at 12 h, 24 h and 48 h after addition of ADH-1 (0.25 mg/ml; 0.50 mg/ml and 1 mg/ml).

    Techniques: In Vitro, Cell Viability Assay, Standard Deviation

    Linc00659 knockdown conferred colon cancer cell drug sensitivity. a HCT116 cells with shLinc00659#1 knockdown were exposed to various concentration (0, 1.3, 2.5 and 5.0 μg/ml) of oxaliplatin(left panel), 5-FU(middle panel), and irinotecan(right panel) for 48 h, respectively. Cell viability was then examined using CellTiter-Glo One Solution cell proliferation assay. b and c After exposure to 5 μg/mL oxaliplatin, 5-FU, and irinotecan for 48 h, the cell cycle distribution was examined by an image flow cytometry assay, and the percentage of sub-G1 was quantified in HCT116 cells with shLinc00659#1 knockdown and in control cells. d and e Cells were stained with both propidium iodide and Annexin V; apoptotic cells were analyzed by an image flow assay, and the percentage of apoptotic cells was quantified. 5-FU, 5-fluorouracil

    Journal: Molecular Cancer

    Article Title: Linc00659, a long noncoding RNA, acts as novel oncogene in regulating cancer cell growth in colorectal cancer

    doi: 10.1186/s12943-018-0821-1

    Figure Lengend Snippet: Linc00659 knockdown conferred colon cancer cell drug sensitivity. a HCT116 cells with shLinc00659#1 knockdown were exposed to various concentration (0, 1.3, 2.5 and 5.0 μg/ml) of oxaliplatin(left panel), 5-FU(middle panel), and irinotecan(right panel) for 48 h, respectively. Cell viability was then examined using CellTiter-Glo One Solution cell proliferation assay. b and c After exposure to 5 μg/mL oxaliplatin, 5-FU, and irinotecan for 48 h, the cell cycle distribution was examined by an image flow cytometry assay, and the percentage of sub-G1 was quantified in HCT116 cells with shLinc00659#1 knockdown and in control cells. d and e Cells were stained with both propidium iodide and Annexin V; apoptotic cells were analyzed by an image flow assay, and the percentage of apoptotic cells was quantified. 5-FU, 5-fluorouracil

    Article Snippet: Cell viability was determined using a CellTiter-Glo One Solution cell proliferation assay (Promega Corporation, Madison, WI, USA), which was performed according to the manufacturer’s instructions.

    Techniques: Concentration Assay, Proliferation Assay, Flow Cytometry, Cytometry, Staining