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Promega celltiter glo luminescent atp assay kit
Effects of APE1 overexpression on mitochondrial function in H/R-treated H9c2 cells. H9c2 cells were transfected with LV-APE1 or LV-Scr and subjected to H/R. (A) The mitochondrial membrane potential was detected using JC-1 staining. (B) Complex I, (C) complex II, (D) complex III and (E) complex IV activities were detected using microplate assay kits. (F) <t>ATP</t> production was assessed using the <t>CellTiter-Glo</t> luminescent ATP assay kit. (G) Bax/Bcl-2 ratio was measured using western blotting and (H) quantified. *P
Celltiter Glo Luminescent Atp Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/celltiter glo luminescent atp assay kit/product/Promega
Average 93 stars, based on 6 article reviews
Price from $9.99 to $1999.99
celltiter glo luminescent atp assay kit - by Bioz Stars, 2020-04
93/100 stars

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1) Product Images from "Apurinic/apyrimidinic endonuclease/redox factor 1 (APE1) alleviates myocardial hypoxia-reoxygenation injury by inhibiting oxidative stress and ameliorating mitochondrial dysfunction"

Article Title: Apurinic/apyrimidinic endonuclease/redox factor 1 (APE1) alleviates myocardial hypoxia-reoxygenation injury by inhibiting oxidative stress and ameliorating mitochondrial dysfunction

Journal: Experimental and Therapeutic Medicine

doi: 10.3892/etm.2019.7212

Effects of APE1 overexpression on mitochondrial function in H/R-treated H9c2 cells. H9c2 cells were transfected with LV-APE1 or LV-Scr and subjected to H/R. (A) The mitochondrial membrane potential was detected using JC-1 staining. (B) Complex I, (C) complex II, (D) complex III and (E) complex IV activities were detected using microplate assay kits. (F) ATP production was assessed using the CellTiter-Glo luminescent ATP assay kit. (G) Bax/Bcl-2 ratio was measured using western blotting and (H) quantified. *P
Figure Legend Snippet: Effects of APE1 overexpression on mitochondrial function in H/R-treated H9c2 cells. H9c2 cells were transfected with LV-APE1 or LV-Scr and subjected to H/R. (A) The mitochondrial membrane potential was detected using JC-1 staining. (B) Complex I, (C) complex II, (D) complex III and (E) complex IV activities were detected using microplate assay kits. (F) ATP production was assessed using the CellTiter-Glo luminescent ATP assay kit. (G) Bax/Bcl-2 ratio was measured using western blotting and (H) quantified. *P

Techniques Used: Over Expression, Transfection, Staining, ATP Assay, Western Blot

Related Articles

Luciferase:

Article Title: Hypoxia-reoxygenation of primary astrocytes results in a redistribution of mitochondrial size and mitophagy
Article Snippet: Mitochondrial ATP production was quantified using the CellTiter-Glo luminescent ATP assay kit (Promega, Madison WI) according to manufacturer’s guidelines. .. Luciferase luminescence was measured with a plate reader (BioTek).

Article Title: Inflammatory response of microglial BV-2 cells includes a glycolytic shift and is modulated by mitochondrial glucose-regulated protein 75/mortalin
Article Snippet: .. Cellular ATP concentrations after 3 h of LPS treatment were measured using the CellTiter-Glo luminescent ATP assay kit (Promega, Madison, WI), based on the luciferase/luciferin reaction. .. A Veritas luminescence counter (Turner BioSystems, Sunnyvale, CA) was used to measure the luminescence signal.

Article Title: Overexpression of mitochondrial Hsp70/Hsp75 protects astrocytes against ischemic injury in vitro
Article Snippet: .. Cellular ATP concentrations were measured using the CellTiter-Glo luminescent ATP assay kit, based on the luciferase/luciferin reaction (Promega, Madison, WI, USA) according to the manufacturer’s instructions. .. A Veritas luminescence counter (Turner BioSystems, Sunnyvale, CA, USA) was used to measure the luminescence signal of the samples in opaque white 96-well plates.

Article Title: Overexpression of mitochondrial Hsp70/Hsp75 in rat brain protects mitochondria, reduces oxidative stress, and protects from focal ischemia
Article Snippet: .. Cellular ATP concentrations were measured using the CellTiter-Glo luminescent ATP assay kit, based on the luciferase/luciferin reaction (Promega, Madison, WI, USA) according to the manufacturer's instructions. .. The luminescence measurements of the samples in opaque white 96-well plates were performed using a Veritas luminescence counter (Turner BioSystems, Sunnyvale, CA, USA).

Article Title: Inhibition of Mitochondrial Function in Astrocytes: Implications for Neuroprotection
Article Snippet: .. Cellular ATP concentrations were measured using the CellTiter-Glo luminescent ATP assay kit, based on the luciferase/luciferin reaction, from Promega (Madison, WI) according to the manufacturer’s instructions. .. A Veritas luminescence counter (Turner BioSystems) was used to measure the luminescence signal of the samples in opaque white 96-well plates.

ATP Assay:

Article Title: Apurinic/apyrimidinic endonuclease/redox factor 1 (APE1) alleviates myocardial hypoxia-reoxygenation injury by inhibiting oxidative stress and ameliorating mitochondrial dysfunction
Article Snippet: .. ATP generation was detected using a CellTiter-Glo luminescent ATP assay kit (Promega Corp., Madison, WI, USA) according to the manufacturer's protocol. ..

Article Title: Hypoxia-reoxygenation of primary astrocytes results in a redistribution of mitochondrial size and mitophagy
Article Snippet: .. Mitochondrial ATP production was quantified using the CellTiter-Glo luminescent ATP assay kit (Promega, Madison WI) according to manufacturer’s guidelines. ..

Article Title: Inflammatory response of microglial BV-2 cells includes a glycolytic shift and is modulated by mitochondrial glucose-regulated protein 75/mortalin
Article Snippet: .. Cellular ATP concentrations after 3 h of LPS treatment were measured using the CellTiter-Glo luminescent ATP assay kit (Promega, Madison, WI), based on the luciferase/luciferin reaction. .. A Veritas luminescence counter (Turner BioSystems, Sunnyvale, CA) was used to measure the luminescence signal.

Article Title: Acrolein cytotoxicity in hepatocytes involves endoplasmic reticulum stress, mitochondrial dysfunction and oxidative stress
Article Snippet: .. Cellular ATP levels were measured using a commercial CellTiter-Glo® Luminescent ATP Assay kit (Promega Corporation, Madison, WI) in accordance with manufacturer instructions. .. Total RNA was isolated using TRIzol (Invitrogen, Carlsbad, CA) and subjected to real time PCR using SYBR green I dye reagents with an ABI prism 7500 sequence detection system (Applied Biosystems, Foster City, CA).

Article Title: Overexpression of mitochondrial Hsp70/Hsp75 protects astrocytes against ischemic injury in vitro
Article Snippet: .. Cellular ATP concentrations were measured using the CellTiter-Glo luminescent ATP assay kit, based on the luciferase/luciferin reaction (Promega, Madison, WI, USA) according to the manufacturer’s instructions. .. A Veritas luminescence counter (Turner BioSystems, Sunnyvale, CA, USA) was used to measure the luminescence signal of the samples in opaque white 96-well plates.

Article Title: Overexpression of mitochondrial Hsp70/Hsp75 in rat brain protects mitochondria, reduces oxidative stress, and protects from focal ischemia
Article Snippet: .. Cellular ATP concentrations were measured using the CellTiter-Glo luminescent ATP assay kit, based on the luciferase/luciferin reaction (Promega, Madison, WI, USA) according to the manufacturer's instructions. .. The luminescence measurements of the samples in opaque white 96-well plates were performed using a Veritas luminescence counter (Turner BioSystems, Sunnyvale, CA, USA).

Article Title: Inhibition of Mitochondrial Function in Astrocytes: Implications for Neuroprotection
Article Snippet: .. Cellular ATP concentrations were measured using the CellTiter-Glo luminescent ATP assay kit, based on the luciferase/luciferin reaction, from Promega (Madison, WI) according to the manufacturer’s instructions. .. A Veritas luminescence counter (Turner BioSystems) was used to measure the luminescence signal of the samples in opaque white 96-well plates.

Bicinchoninic Acid Protein Assay:

Article Title: Overexpression of mitochondrial Hsp70/Hsp75 protects astrocytes against ischemic injury in vitro
Article Snippet: Cellular ATP concentrations were measured using the CellTiter-Glo luminescent ATP assay kit, based on the luciferase/luciferin reaction (Promega, Madison, WI, USA) according to the manufacturer’s instructions. .. Protein concentrations were measured using a bicinchoninic acid protein assay from Pierce per the manufacturer’s instructions.

Activity Assay:

Article Title: Apurinic/apyrimidinic endonuclease/redox factor 1 (APE1) alleviates myocardial hypoxia-reoxygenation injury by inhibiting oxidative stress and ameliorating mitochondrial dysfunction
Article Snippet: Paragraph title: Electron transport chain (ETC) activity chain complex activity and ATP production capacity ... ATP generation was detected using a CellTiter-Glo luminescent ATP assay kit (Promega Corp., Madison, WI, USA) according to the manufacturer's protocol.

BIA-KA:

Article Title: Inflammatory response of microglial BV-2 cells includes a glycolytic shift and is modulated by mitochondrial glucose-regulated protein 75/mortalin
Article Snippet: Cell lysate protein concentrations were measured with the BCA protein assay reagent kit (Pierce, Rockford, IL). .. Cellular ATP concentrations after 3 h of LPS treatment were measured using the CellTiter-Glo luminescent ATP assay kit (Promega, Madison, WI), based on the luciferase/luciferin reaction.

Article Title: Inhibition of Mitochondrial Function in Astrocytes: Implications for Neuroprotection
Article Snippet: Cellular ATP concentrations were measured using the CellTiter-Glo luminescent ATP assay kit, based on the luciferase/luciferin reaction, from Promega (Madison, WI) according to the manufacturer’s instructions. .. Protein concentrations were measured using bicinchoninic acid (BCA) protein assay reagent kit from Pierce (Rockford, IL) per manufacturer’s instructions.

Lactate Assay:

Article Title: Inflammatory response of microglial BV-2 cells includes a glycolytic shift and is modulated by mitochondrial glucose-regulated protein 75/mortalin
Article Snippet: Lactate levels were also independently estimated using the Lactate Assay Kit (Biovision Research, Mountain View, CA). .. Cellular ATP concentrations after 3 h of LPS treatment were measured using the CellTiter-Glo luminescent ATP assay kit (Promega, Madison, WI), based on the luciferase/luciferin reaction.

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  • 93
    Promega celltiter glo luminescent atp assay kit
    Effects of APE1 overexpression on mitochondrial function in H/R-treated H9c2 cells. H9c2 cells were transfected with LV-APE1 or LV-Scr and subjected to H/R. (A) The mitochondrial membrane potential was detected using JC-1 staining. (B) Complex I, (C) complex II, (D) complex III and (E) complex IV activities were detected using microplate assay kits. (F) <t>ATP</t> production was assessed using the <t>CellTiter-Glo</t> luminescent ATP assay kit. (G) Bax/Bcl-2 ratio was measured using western blotting and (H) quantified. *P
    Celltiter Glo Luminescent Atp Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/celltiter glo luminescent atp assay kit/product/Promega
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    celltiter glo luminescent atp assay kit - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    86
    Promega celltiter glo luminescence atp assay kit
    Viability of cells infected with ZP6248 and its mutants. The GHOST GPR15 cells were infected with ZP6248 and its mutants and cultured for 10(day 10) was determined by <t>CellTiter-Glo</t> Luminescent Cell Viability Assay. Each virus was assayed in triplicate and mean ± standard deviation is shown. Similar results were obtained in two independent experiments and the results from one experiment are shown.
    Celltiter Glo Luminescence Atp Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/celltiter glo luminescence atp assay kit/product/Promega
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    celltiter glo luminescence atp assay kit - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Effects of APE1 overexpression on mitochondrial function in H/R-treated H9c2 cells. H9c2 cells were transfected with LV-APE1 or LV-Scr and subjected to H/R. (A) The mitochondrial membrane potential was detected using JC-1 staining. (B) Complex I, (C) complex II, (D) complex III and (E) complex IV activities were detected using microplate assay kits. (F) ATP production was assessed using the CellTiter-Glo luminescent ATP assay kit. (G) Bax/Bcl-2 ratio was measured using western blotting and (H) quantified. *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Apurinic/apyrimidinic endonuclease/redox factor 1 (APE1) alleviates myocardial hypoxia-reoxygenation injury by inhibiting oxidative stress and ameliorating mitochondrial dysfunction

    doi: 10.3892/etm.2019.7212

    Figure Lengend Snippet: Effects of APE1 overexpression on mitochondrial function in H/R-treated H9c2 cells. H9c2 cells were transfected with LV-APE1 or LV-Scr and subjected to H/R. (A) The mitochondrial membrane potential was detected using JC-1 staining. (B) Complex I, (C) complex II, (D) complex III and (E) complex IV activities were detected using microplate assay kits. (F) ATP production was assessed using the CellTiter-Glo luminescent ATP assay kit. (G) Bax/Bcl-2 ratio was measured using western blotting and (H) quantified. *P

    Article Snippet: ATP generation was detected using a CellTiter-Glo luminescent ATP assay kit (Promega Corp., Madison, WI, USA) according to the manufacturer's protocol.

    Techniques: Over Expression, Transfection, Staining, ATP Assay, Western Blot

    Viability of cells infected with ZP6248 and its mutants. The GHOST GPR15 cells were infected with ZP6248 and its mutants and cultured for 10(day 10) was determined by CellTiter-Glo Luminescent Cell Viability Assay. Each virus was assayed in triplicate and mean ± standard deviation is shown. Similar results were obtained in two independent experiments and the results from one experiment are shown.

    Journal: PLoS ONE

    Article Title: The Variable Loop 3 in the Envelope Glycoprotein Is Critical for the Atypical Coreceptor Usage of an HIV-1 Strain

    doi: 10.1371/journal.pone.0098058

    Figure Lengend Snippet: Viability of cells infected with ZP6248 and its mutants. The GHOST GPR15 cells were infected with ZP6248 and its mutants and cultured for 10(day 10) was determined by CellTiter-Glo Luminescent Cell Viability Assay. Each virus was assayed in triplicate and mean ± standard deviation is shown. Similar results were obtained in two independent experiments and the results from one experiment are shown.

    Article Snippet: Cell viability assay Cellular ATP was measured in white 96-well plates using the CellTiter Glo luminescence ATP assay kit (Promega, Madison, WI, USA).

    Techniques: Infection, Cell Culture, Cell Viability Assay, Standard Deviation