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Promega celltiter glo atp measurement system
Cellular antisense activity in HeLa pLuc705 cells of PNAs 4305, 4263, 4265, 4306, 2787 and 2534. After transfection of the cells with 3 µM PNA, irradiation of cells was carried out with the excitation wavelength specific for each fluorophore for 1, 5, 10, 20, 25 and 30 min. Cells were incubated further for 24 h after irradiation. All the samples were subjected to the luciferase analysis. Luciferase activities were analysed by <t>Bright-Glo</t> reagent (Promega) and are expressed as relative light units (RLU/well) normalized by the <t>ATP.</t> Each data set represents the mean ± SD of triplicate experiment. CQ: chloroquine. TM-Rhodamine: Tetramethylrhodamine.
Celltiter Glo Atp Measurement System, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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celltiter glo atp measurement system - by Bioz Stars, 2020-04
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1) Product Images from "Effective photo-enhancement of cellular activity of fluorophore-octaarginine antisense PNA conjugates correlates with singlet oxygen formation, endosomal escape and chromophore lipophilicity"

Article Title: Effective photo-enhancement of cellular activity of fluorophore-octaarginine antisense PNA conjugates correlates with singlet oxygen formation, endosomal escape and chromophore lipophilicity

Journal: Scientific Reports

doi: 10.1038/s41598-017-18947-x

Cellular antisense activity in HeLa pLuc705 cells of PNAs 4305, 4263, 4265, 4306, 2787 and 2534. After transfection of the cells with 3 µM PNA, irradiation of cells was carried out with the excitation wavelength specific for each fluorophore for 1, 5, 10, 20, 25 and 30 min. Cells were incubated further for 24 h after irradiation. All the samples were subjected to the luciferase analysis. Luciferase activities were analysed by Bright-Glo reagent (Promega) and are expressed as relative light units (RLU/well) normalized by the ATP. Each data set represents the mean ± SD of triplicate experiment. CQ: chloroquine. TM-Rhodamine: Tetramethylrhodamine.
Figure Legend Snippet: Cellular antisense activity in HeLa pLuc705 cells of PNAs 4305, 4263, 4265, 4306, 2787 and 2534. After transfection of the cells with 3 µM PNA, irradiation of cells was carried out with the excitation wavelength specific for each fluorophore for 1, 5, 10, 20, 25 and 30 min. Cells were incubated further for 24 h after irradiation. All the samples were subjected to the luciferase analysis. Luciferase activities were analysed by Bright-Glo reagent (Promega) and are expressed as relative light units (RLU/well) normalized by the ATP. Each data set represents the mean ± SD of triplicate experiment. CQ: chloroquine. TM-Rhodamine: Tetramethylrhodamine.

Techniques Used: Activity Assay, Transfection, Irradiation, Incubation, Luciferase

Related Articles

Irradiation:

Article Title: Effective photo-enhancement of cellular activity of fluorophore-octaarginine antisense PNA conjugates correlates with singlet oxygen formation, endosomal escape and chromophore lipophilicity
Article Snippet: At day three (24 h after irradiation) cells were lysed with passive lysis buffer (Promega) and subjected to the luciferase activity analysis by using the Bright-Glo luciferase assay system (Promega) based on the manufacturer’s protocol. .. Luminescent readings obtained by the Bright-Glo luciferase assay system were background-subtracted and normalized for the cell viability by the CellTiter-Glo ATP measurement system (Promega) based on the manufacturer protocol.

Incubation:

Article Title: Effective photo-enhancement of cellular activity of fluorophore-octaarginine antisense PNA conjugates correlates with singlet oxygen formation, endosomal escape and chromophore lipophilicity
Article Snippet: Luciferase activity assay Cells were seeded in 96 well plates, incubated with PNA and then subjected to irradiation (0–30 min) using the LED projector lamp light source. .. Luminescent readings obtained by the Bright-Glo luciferase assay system were background-subtracted and normalized for the cell viability by the CellTiter-Glo ATP measurement system (Promega) based on the manufacturer protocol.

Luciferase:

Article Title: Effective photo-enhancement of cellular activity of fluorophore-octaarginine antisense PNA conjugates correlates with singlet oxygen formation, endosomal escape and chromophore lipophilicity
Article Snippet: .. Luminescent readings obtained by the Bright-Glo luciferase assay system were background-subtracted and normalized for the cell viability by the CellTiter-Glo ATP measurement system (Promega) based on the manufacturer protocol. .. Cytotoxicity Supernatants from the 96-well plates of the luciferase activity assay (previous section) were collected and subjected to cytotoxicity test.

Activity Assay:

Article Title: Effective photo-enhancement of cellular activity of fluorophore-octaarginine antisense PNA conjugates correlates with singlet oxygen formation, endosomal escape and chromophore lipophilicity
Article Snippet: Paragraph title: Luciferase activity assay ... Luminescent readings obtained by the Bright-Glo luciferase assay system were background-subtracted and normalized for the cell viability by the CellTiter-Glo ATP measurement system (Promega) based on the manufacturer protocol.

Lysis:

Article Title: Effective photo-enhancement of cellular activity of fluorophore-octaarginine antisense PNA conjugates correlates with singlet oxygen formation, endosomal escape and chromophore lipophilicity
Article Snippet: At day three (24 h after irradiation) cells were lysed with passive lysis buffer (Promega) and subjected to the luciferase activity analysis by using the Bright-Glo luciferase assay system (Promega) based on the manufacturer’s protocol. .. Luminescent readings obtained by the Bright-Glo luciferase assay system were background-subtracted and normalized for the cell viability by the CellTiter-Glo ATP measurement system (Promega) based on the manufacturer protocol.

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    Promega celltiter glo atp measurement system
    Cellular antisense activity in HeLa pLuc705 cells of PNAs 4305, 4263, 4265, 4306, 2787 and 2534. After transfection of the cells with 3 µM PNA, irradiation of cells was carried out with the excitation wavelength specific for each fluorophore for 1, 5, 10, 20, 25 and 30 min. Cells were incubated further for 24 h after irradiation. All the samples were subjected to the luciferase analysis. Luciferase activities were analysed by <t>Bright-Glo</t> reagent (Promega) and are expressed as relative light units (RLU/well) normalized by the <t>ATP.</t> Each data set represents the mean ± SD of triplicate experiment. CQ: chloroquine. TM-Rhodamine: Tetramethylrhodamine.
    Celltiter Glo Atp Measurement System, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/celltiter glo atp measurement system/product/Promega
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    celltiter glo atp measurement system - by Bioz Stars, 2020-04
    93/100 stars
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    Cellular antisense activity in HeLa pLuc705 cells of PNAs 4305, 4263, 4265, 4306, 2787 and 2534. After transfection of the cells with 3 µM PNA, irradiation of cells was carried out with the excitation wavelength specific for each fluorophore for 1, 5, 10, 20, 25 and 30 min. Cells were incubated further for 24 h after irradiation. All the samples were subjected to the luciferase analysis. Luciferase activities were analysed by Bright-Glo reagent (Promega) and are expressed as relative light units (RLU/well) normalized by the ATP. Each data set represents the mean ± SD of triplicate experiment. CQ: chloroquine. TM-Rhodamine: Tetramethylrhodamine.

    Journal: Scientific Reports

    Article Title: Effective photo-enhancement of cellular activity of fluorophore-octaarginine antisense PNA conjugates correlates with singlet oxygen formation, endosomal escape and chromophore lipophilicity

    doi: 10.1038/s41598-017-18947-x

    Figure Lengend Snippet: Cellular antisense activity in HeLa pLuc705 cells of PNAs 4305, 4263, 4265, 4306, 2787 and 2534. After transfection of the cells with 3 µM PNA, irradiation of cells was carried out with the excitation wavelength specific for each fluorophore for 1, 5, 10, 20, 25 and 30 min. Cells were incubated further for 24 h after irradiation. All the samples were subjected to the luciferase analysis. Luciferase activities were analysed by Bright-Glo reagent (Promega) and are expressed as relative light units (RLU/well) normalized by the ATP. Each data set represents the mean ± SD of triplicate experiment. CQ: chloroquine. TM-Rhodamine: Tetramethylrhodamine.

    Article Snippet: Luminescent readings obtained by the Bright-Glo luciferase assay system were background-subtracted and normalized for the cell viability by the CellTiter-Glo ATP measurement system (Promega) based on the manufacturer protocol.

    Techniques: Activity Assay, Transfection, Irradiation, Incubation, Luciferase