cells mscs  (Thermo Fisher)


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    Name:
    StemPro BM Mesenchymal Stem Cells
    Description:
    StemPro BM Mesenchymal Stem Cells are cryopreserved human bone marrow mesenchymal stem cells BM MSCs These Off the Shelf cells are manufactured to meet Good Manufacturing Practice GMP manufacturing standards in a California licensed facility and available for Research Use Only They are isolated and expanded in culture to passage 4 under a low oxygen ischemia tolerant it proprietary clinical scale manufacturing process using Bovine Growth Serum BGS supplemented media and cryopreserved in an animal origin free cryopreservation medium The cells are supplied at 1x106 and 5x106 viable cells vial A Certificate of Analysis is available for each lot which documents the quality control specifications test results and donor information StemPro BM Mesenchymal Stem Cells allow you to • Minimize assay variability with itMSCs manufactured in compliance with GMP manufacturing standards• Improve functionality studies with immature high potency MSCs• No observed tumorigenicity and toxicity in GLP compliant animal studies• Accommodate experiments requiring one large lot of MSCs• Minimize your chance of assay failure when using tested cells reagents and protocolsImmature High Potency Low Oxygen Cultured MSCsStemPro BM Mesenchymal Stem Cells are derived from human bone marrow aspirates from qualified traceable donors The cells are isolated cultured and expanded in compliance with GMP manufacturing standards in a California licensed facility using a proprietary clinical scale Reduced Oxygen Tension manufacturing process Cells manufactured in reduced oxygen tension environments result in higher cell yields of highly potent immature stem cells compared to cells expanded in normal oxygen culture conditions Proven Non toxic ProfilesCells have demonstrated to be non toxic and non tumorigenic in GLP animal studies which were performed according to FDA guidelines A large lot can be made available for those requiring assay standardization Protocols using tested reagents will be provided to help minimize your chance of assay failure when using reagents and cells that have not been evaluated to work together View our tested media and reagents High Post Cryopreservation ViabilityStemPro Mesenchymal Stem Cells are supplied in CryoStor cryopreservation medium at 1x106 and 5x106 viable cells vial This medium is animal origin free and shown to support high cell viabilities post thawing Controlled processes are used for cryopreservation handling and shipping to help minimize risk of adverse effects to the quality of the cells Made in USA by Stemedica for Thermo Fisher Scientific
    Catalog Number:
    A15652
    Price:
    None
    Category:
    Eukaryotic Cells
    Applications:
    Cell Culture|Mesenchymal Stem Cell Culture|Stem Cell Culture|Stem Cell Research
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    Structured Review

    Thermo Fisher cells mscs
    Gene expression analysis for fold-change of chondrogenic markers in physioxia relative to hyperoxia demonstrates that high-GAG groups of both <t>MSCs</t> and <t>ACPs</t> are highly responsive to oxygen level and upregulate a majority of genes representative of the articular cartilage phenotype in low-oxygen environments. Data are mean ± standard deviation of fold-change in gene expression for each group ( n = 8). Statistical significance defined as * p
    StemPro BM Mesenchymal Stem Cells are cryopreserved human bone marrow mesenchymal stem cells BM MSCs These Off the Shelf cells are manufactured to meet Good Manufacturing Practice GMP manufacturing standards in a California licensed facility and available for Research Use Only They are isolated and expanded in culture to passage 4 under a low oxygen ischemia tolerant it proprietary clinical scale manufacturing process using Bovine Growth Serum BGS supplemented media and cryopreserved in an animal origin free cryopreservation medium The cells are supplied at 1x106 and 5x106 viable cells vial A Certificate of Analysis is available for each lot which documents the quality control specifications test results and donor information StemPro BM Mesenchymal Stem Cells allow you to • Minimize assay variability with itMSCs manufactured in compliance with GMP manufacturing standards• Improve functionality studies with immature high potency MSCs• No observed tumorigenicity and toxicity in GLP compliant animal studies• Accommodate experiments requiring one large lot of MSCs• Minimize your chance of assay failure when using tested cells reagents and protocolsImmature High Potency Low Oxygen Cultured MSCsStemPro BM Mesenchymal Stem Cells are derived from human bone marrow aspirates from qualified traceable donors The cells are isolated cultured and expanded in compliance with GMP manufacturing standards in a California licensed facility using a proprietary clinical scale Reduced Oxygen Tension manufacturing process Cells manufactured in reduced oxygen tension environments result in higher cell yields of highly potent immature stem cells compared to cells expanded in normal oxygen culture conditions Proven Non toxic ProfilesCells have demonstrated to be non toxic and non tumorigenic in GLP animal studies which were performed according to FDA guidelines A large lot can be made available for those requiring assay standardization Protocols using tested reagents will be provided to help minimize your chance of assay failure when using reagents and cells that have not been evaluated to work together View our tested media and reagents High Post Cryopreservation ViabilityStemPro Mesenchymal Stem Cells are supplied in CryoStor cryopreservation medium at 1x106 and 5x106 viable cells vial This medium is animal origin free and shown to support high cell viabilities post thawing Controlled processes are used for cryopreservation handling and shipping to help minimize risk of adverse effects to the quality of the cells Made in USA by Stemedica for Thermo Fisher Scientific
    https://www.bioz.com/result/cells mscs/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cells mscs - by Bioz Stars, 2021-09
    99/100 stars

    Images

    1) Product Images from "Responses to altered oxygen tension are distinct between human stem cells of high and low chondrogenic capacity"

    Article Title: Responses to altered oxygen tension are distinct between human stem cells of high and low chondrogenic capacity

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-016-0419-8

    Gene expression analysis for fold-change of chondrogenic markers in physioxia relative to hyperoxia demonstrates that high-GAG groups of both MSCs and ACPs are highly responsive to oxygen level and upregulate a majority of genes representative of the articular cartilage phenotype in low-oxygen environments. Data are mean ± standard deviation of fold-change in gene expression for each group ( n = 8). Statistical significance defined as * p
    Figure Legend Snippet: Gene expression analysis for fold-change of chondrogenic markers in physioxia relative to hyperoxia demonstrates that high-GAG groups of both MSCs and ACPs are highly responsive to oxygen level and upregulate a majority of genes representative of the articular cartilage phenotype in low-oxygen environments. Data are mean ± standard deviation of fold-change in gene expression for each group ( n = 8). Statistical significance defined as * p

    Techniques Used: Expressing, Standard Deviation

    a Representative toluidine blue stain for total proteoglycans demonstrates smaller pellets with less metachromasia for low-GAG MSC preparations and ACP clones in both hyperoxia and physioxia relative to paired high-GAG MSC preparations and ACP clones at the respective oxygen levels. Images were acquired with bright-field microscopy, scale bars = 100 μm. b Measurement of pellet diameter revealed a statistically significant difference in pellet size between both MSCs and ACPs of high or low chondrogenicity and between high-GAG MSCs at physioxia or hyperoxia. Statistical significance defined as * p
    Figure Legend Snippet: a Representative toluidine blue stain for total proteoglycans demonstrates smaller pellets with less metachromasia for low-GAG MSC preparations and ACP clones in both hyperoxia and physioxia relative to paired high-GAG MSC preparations and ACP clones at the respective oxygen levels. Images were acquired with bright-field microscopy, scale bars = 100 μm. b Measurement of pellet diameter revealed a statistically significant difference in pellet size between both MSCs and ACPs of high or low chondrogenicity and between high-GAG MSCs at physioxia or hyperoxia. Statistical significance defined as * p

    Techniques Used: Staining, Clone Assay, Microscopy

    Clustering of oxygen-dependent gene expression based on z -score demonstrates that groups of MSCs and ACPs are more similar between GAG level than within cell type in response to culture in physioxia relative to culture in hyperoxia. ACP articular cartilage progenitor, GAG glycosaminoglycan, MSC mesenchymal stem cell
    Figure Legend Snippet: Clustering of oxygen-dependent gene expression based on z -score demonstrates that groups of MSCs and ACPs are more similar between GAG level than within cell type in response to culture in physioxia relative to culture in hyperoxia. ACP articular cartilage progenitor, GAG glycosaminoglycan, MSC mesenchymal stem cell

    Techniques Used: Expressing

    Gene expression analysis for fold-change of chondrogenic markers of the articular cartilage phenotype ( COL2A1 , ACAN ), the fibrocartilaginous phenotype ( COL1A1 ), and the hypertrophic phenotype ( COL10A1 , MMP13 ) demonstrates varied chondrogenic responses by high-GAG and low-GAG groups of each cell type, MSCs and ACPs, during pellet culture in physioxic relative to hyperoxic conditions. Data are mean ± standard deviation of fold-change for each group ( n = 6–10). Statistical significance defined as * p
    Figure Legend Snippet: Gene expression analysis for fold-change of chondrogenic markers of the articular cartilage phenotype ( COL2A1 , ACAN ), the fibrocartilaginous phenotype ( COL1A1 ), and the hypertrophic phenotype ( COL10A1 , MMP13 ) demonstrates varied chondrogenic responses by high-GAG and low-GAG groups of each cell type, MSCs and ACPs, during pellet culture in physioxic relative to hyperoxic conditions. Data are mean ± standard deviation of fold-change for each group ( n = 6–10). Statistical significance defined as * p

    Techniques Used: Expressing, Standard Deviation

    Related Articles

    other:

    Article Title: Stem cells in end-to-side neurorrhaphy. Experimental study in rats 1
    Article Snippet: The culture was performed according to a pre-established protocol in the Molecular Biology and Cell Engineering Laboratory for mesenchymal stem cells obtained from the adipose tissue.

    Flow Cytometry:

    Article Title: Comparative Analysis of Tenogenic Gene Expression in Tenocyte-Derived Induced Pluripotent Stem Cells and Bone Marrow-Derived Mesenchymal Stem Cells in Response to Biochemical and Biomechanical Stimuli
    Article Snippet: .. Characterization of mesenchymal stem cell was carried out by flow cytometry with positive expression of CD29, CD44, CD90, CD105, and MHC-I and with negative expression of CD45, CD79, and MHC-II. ..

    Expressing:

    Article Title: Comparative Analysis of Tenogenic Gene Expression in Tenocyte-Derived Induced Pluripotent Stem Cells and Bone Marrow-Derived Mesenchymal Stem Cells in Response to Biochemical and Biomechanical Stimuli
    Article Snippet: .. Characterization of mesenchymal stem cell was carried out by flow cytometry with positive expression of CD29, CD44, CD90, CD105, and MHC-I and with negative expression of CD45, CD79, and MHC-II. ..

    Purification:

    Article Title: Exercise reduced the formation of new adipocytes in the adipose tissue of mice in vivo
    Article Snippet: .. The adipocytes were purified by incubation with a cocktail of antibodies against markers of endothelial cells (CD31- eBioscience), hematopoietic cells (CD45- BioLegend), and mesenchymal stem cells (CD34- eBioscience) for 15min at room temperature. ..

    Incubation:

    Article Title: Exercise reduced the formation of new adipocytes in the adipose tissue of mice in vivo
    Article Snippet: .. The adipocytes were purified by incubation with a cocktail of antibodies against markers of endothelial cells (CD31- eBioscience), hematopoietic cells (CD45- BioLegend), and mesenchymal stem cells (CD34- eBioscience) for 15min at room temperature. ..

    Mouse Assay:

    Article Title: Reestablishment of Redox Homeostasis in the Nociceptive Primary Afferent as a Mechanism of Antinociception Promoted by Mesenchymal Stem/Stromal Cells in Oxaliplatin-Induced Chronic Peripheral Neuropathy
    Article Snippet: .. Production of Mesenchymal Stem/Stromal Cells (MSC) MSC were obtained from the bone marrow of femurs and tibiae of male C57Bl/6 mice, following the methods described by Soleimani and Nadri [ ]. ..

    Isolation:

    Article Title: Inhibition of TGFβ improves hematopoietic stem cell niche and ameliorates cancer-related anemia
    Article Snippet: .. Isolation of mesenchymal stromal cells Mesenchymal stromal cells (MSC) were isolated as reported [ ]. ..

    Modification:

    Article Title: Extracorporeal shock wave therapy combined with engineered mesenchymal stem cells expressing stromal cell-derived factor-1 can improve erectile dysfunction in streptozotocin-induced diabetic rats
    Article Snippet: .. The culture of bone marrow mesenchymal stem cells (BM-MSCs) were done in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, Gaithersburg, MD, USA) supplemented with 20% fetal bovine serum (FBS; Gibco) and 5 ng/mL basic fibroblast growth factor (bFGF; Cell Signaling Technology, Danvers, MA, USA) at 37 °C with 5% CO2 . ..

    Cell Culture:

    Article Title: Stem cells in end-to-side neurorrhaphy. Experimental study in rats 1
    Article Snippet: .. A random sample of mesenchymal stem cells was cultured for differentiation in the adipogenic, osteogenic and chondrogenic lines. ..

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    Thermo Fisher bm mscs
    <t>rAMF</t> increases the in vitro chemotaxis of <t>MSCs</t> towards HCC and their adhesion to endothelial cells. A) Pretreatment of BM-MSCs with 1 µg/ml rAMF (black bars) increases chemotaxis towards TCM derived from HuH7 or HC-PT-5 cells compared to untreated cells (white bars). B) Wound-healing assay of MSCs after pretreatment with rAMF or control (DMEM). Representative images were taken 24 hours after scratching. C) Adhesion to HMEC-1 endothelial cells was increased in BM-MSCs exposed to rAMF. D) Expression of AMF receptor (AMFR), GDP dissociation inhibitor 2 (GDI-1), caveolin-1 (CAV-1) and caveolin-2 (CAV-2) by qRT-PCR. *p
    Bm Mscs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    rAMF increases the in vitro chemotaxis of MSCs towards HCC and their adhesion to endothelial cells. A) Pretreatment of BM-MSCs with 1 µg/ml rAMF (black bars) increases chemotaxis towards TCM derived from HuH7 or HC-PT-5 cells compared to untreated cells (white bars). B) Wound-healing assay of MSCs after pretreatment with rAMF or control (DMEM). Representative images were taken 24 hours after scratching. C) Adhesion to HMEC-1 endothelial cells was increased in BM-MSCs exposed to rAMF. D) Expression of AMF receptor (AMFR), GDP dissociation inhibitor 2 (GDI-1), caveolin-1 (CAV-1) and caveolin-2 (CAV-2) by qRT-PCR. *p

    Journal: PLoS ONE

    Article Title: Increased Migration of Human Mesenchymal Stromal Cells by Autocrine Motility Factor (AMF) Resulted in Enhanced Recruitment towards Hepatocellular Carcinoma

    doi: 10.1371/journal.pone.0095171

    Figure Lengend Snippet: rAMF increases the in vitro chemotaxis of MSCs towards HCC and their adhesion to endothelial cells. A) Pretreatment of BM-MSCs with 1 µg/ml rAMF (black bars) increases chemotaxis towards TCM derived from HuH7 or HC-PT-5 cells compared to untreated cells (white bars). B) Wound-healing assay of MSCs after pretreatment with rAMF or control (DMEM). Representative images were taken 24 hours after scratching. C) Adhesion to HMEC-1 endothelial cells was increased in BM-MSCs exposed to rAMF. D) Expression of AMF receptor (AMFR), GDP dissociation inhibitor 2 (GDI-1), caveolin-1 (CAV-1) and caveolin-2 (CAV-2) by qRT-PCR. *p

    Article Snippet: For in vivo migration studies, BM-MSCs or BM-MSCs pretreated with rAMF (5×105 ) were prelabeled with CMDiI for histological analysis and DiR (Molecular Probes, Invitrogen) for fluorescence imaging (FI).

    Techniques: In Vitro, Chemotaxis Assay, Derivative Assay, Wound Healing Assay, Expressing, Quantitative RT-PCR

    rAMF increases the in vivo migration and anchorage of MSCs to HCC tumors. BM-MSCs prestimulated with 1 µg/ml of rAMF were labeled with DiR and CMDiI cell trackers and IV injected in SC HuH7 tumor-bearing mice. After 3 days, tumors were removed and exposed to FI. A) Total FI was calculated by measuring the region of interest (ROI) for all the tissues isolated and the results were expressed as total radiant efficiency. ns, non significant. B) Representative tumor images of mice inoculated with rAMF-prestimulated BM-MSCs (MSC-rAMF) or unstimulated cells (MSCs). Images represent the average radiant efficiency. Region of interest (ROI) was calculated for the isolated tumor (C), liver (D), lung (E) and spleen (F) and the results were expressed as the average radiant efficiency. **p

    Journal: PLoS ONE

    Article Title: Increased Migration of Human Mesenchymal Stromal Cells by Autocrine Motility Factor (AMF) Resulted in Enhanced Recruitment towards Hepatocellular Carcinoma

    doi: 10.1371/journal.pone.0095171

    Figure Lengend Snippet: rAMF increases the in vivo migration and anchorage of MSCs to HCC tumors. BM-MSCs prestimulated with 1 µg/ml of rAMF were labeled with DiR and CMDiI cell trackers and IV injected in SC HuH7 tumor-bearing mice. After 3 days, tumors were removed and exposed to FI. A) Total FI was calculated by measuring the region of interest (ROI) for all the tissues isolated and the results were expressed as total radiant efficiency. ns, non significant. B) Representative tumor images of mice inoculated with rAMF-prestimulated BM-MSCs (MSC-rAMF) or unstimulated cells (MSCs). Images represent the average radiant efficiency. Region of interest (ROI) was calculated for the isolated tumor (C), liver (D), lung (E) and spleen (F) and the results were expressed as the average radiant efficiency. **p

    Article Snippet: For in vivo migration studies, BM-MSCs or BM-MSCs pretreated with rAMF (5×105 ) were prelabeled with CMDiI for histological analysis and DiR (Molecular Probes, Invitrogen) for fluorescence imaging (FI).

    Techniques: In Vivo, Migration, Labeling, Injection, Mouse Assay, Isolation

    AMF potently stimulates in vitro chemotaxis of MSCs from different sources. A) Detection of AMF (55 kDa) by western blot in CCM derived from HCC cells and TCM from ex vivo HCC SC tumors (upper panel). Colloidal Coomassie staining was performed as loading control (lower panel). MSC migration was analyzed with a Boyden chamber assay using rAMF as chemoattractant for BM-MSCs (B), HUCPVCs (C) or AT-MSCs (D). Results were expressed as percentage of control (DMEM) ±SEM. *p

    Journal: PLoS ONE

    Article Title: Increased Migration of Human Mesenchymal Stromal Cells by Autocrine Motility Factor (AMF) Resulted in Enhanced Recruitment towards Hepatocellular Carcinoma

    doi: 10.1371/journal.pone.0095171

    Figure Lengend Snippet: AMF potently stimulates in vitro chemotaxis of MSCs from different sources. A) Detection of AMF (55 kDa) by western blot in CCM derived from HCC cells and TCM from ex vivo HCC SC tumors (upper panel). Colloidal Coomassie staining was performed as loading control (lower panel). MSC migration was analyzed with a Boyden chamber assay using rAMF as chemoattractant for BM-MSCs (B), HUCPVCs (C) or AT-MSCs (D). Results were expressed as percentage of control (DMEM) ±SEM. *p

    Article Snippet: For in vivo migration studies, BM-MSCs or BM-MSCs pretreated with rAMF (5×105 ) were prelabeled with CMDiI for histological analysis and DiR (Molecular Probes, Invitrogen) for fluorescence imaging (FI).

    Techniques: In Vitro, Chemotaxis Assay, Western Blot, Derivative Assay, Ex Vivo, Staining, Migration, Boyden Chamber Assay

    MMP3 expression and MMP2 activity is induced in MSCs by rAMF. A) Analysis of MMP3 expression by qRT-PCR in BM-MSCs (black bars), HUCPVCs (white bars) or AT-MSCs (gray bars) stimulated with 1 µg/ml of rAMF. **p

    Journal: PLoS ONE

    Article Title: Increased Migration of Human Mesenchymal Stromal Cells by Autocrine Motility Factor (AMF) Resulted in Enhanced Recruitment towards Hepatocellular Carcinoma

    doi: 10.1371/journal.pone.0095171

    Figure Lengend Snippet: MMP3 expression and MMP2 activity is induced in MSCs by rAMF. A) Analysis of MMP3 expression by qRT-PCR in BM-MSCs (black bars), HUCPVCs (white bars) or AT-MSCs (gray bars) stimulated with 1 µg/ml of rAMF. **p

    Article Snippet: For in vivo migration studies, BM-MSCs or BM-MSCs pretreated with rAMF (5×105 ) were prelabeled with CMDiI for histological analysis and DiR (Molecular Probes, Invitrogen) for fluorescence imaging (FI).

    Techniques: Expressing, Activity Assay, Quantitative RT-PCR

    Effects of gelatin-coated matrix on the differentiation potential of BM-MSCs. By passage 5, BM-MSCs were cultured on dishes coated without or with 1% (wt/v) gelatin. (a) For analysis of chondrogenic differentiation potential, BM-MSCs were cultured on a 1% (wt/v) gelatin matrix using a chondrogenesis differentiation kit for 3 weeks, and differentiation into chondrocytes was assessed by alcian blue staining. (b) For analysis of adipogenic differentiation potential, BM-MSCs were incubated for 3 weeks in adipogenic differentiation medium. BM-MSCs that differentiated into adipocytes were identified by Oil red O staining and quantified by measuring the absorbance at 490 nm. There was no significant difference in adipogenic differentiation potential between BM-MSCs cultured without and with 1% (wt/v) gelatin. (c) For analysis of osteogenic differentiation potential, BM-MSCs were incubated in osteogenic differentiation medium for 2 weeks. BM-MSCs that differentiated into osteoblasts were identified by alizarin red staining (ARS), which stains calcium deposits in the differentiated cells, and quantified by measuring the absorbance at 550 nm. There was no significant difference in osteogenic differentiation potential between BM-MSCs cultured with and without 1% (wt/v) gelatin. (d) For analysis of neurogenic differentiation potential, BM-MSCs were cultured for 7 days in neurogenic differentiation medium. BM-MSCs that differentiated into neurogenic cells were stained with a fluorescence-conjugated primary antibody against Nestin (neural lineage marker) and assessed by flow cytometry. A significantly higher percentage of Nestin-positive cells was detected in BM-MSCs cultured on 1% (wt/v) gelatin matrix than in those cultured on plates without gelatin. All data shown are means ± S.D. of three (d) or four ((b) and (c)) independent experiments. * P

    Journal: BioMed Research International

    Article Title: Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated Matrix

    doi: 10.1155/2013/347618

    Figure Lengend Snippet: Effects of gelatin-coated matrix on the differentiation potential of BM-MSCs. By passage 5, BM-MSCs were cultured on dishes coated without or with 1% (wt/v) gelatin. (a) For analysis of chondrogenic differentiation potential, BM-MSCs were cultured on a 1% (wt/v) gelatin matrix using a chondrogenesis differentiation kit for 3 weeks, and differentiation into chondrocytes was assessed by alcian blue staining. (b) For analysis of adipogenic differentiation potential, BM-MSCs were incubated for 3 weeks in adipogenic differentiation medium. BM-MSCs that differentiated into adipocytes were identified by Oil red O staining and quantified by measuring the absorbance at 490 nm. There was no significant difference in adipogenic differentiation potential between BM-MSCs cultured without and with 1% (wt/v) gelatin. (c) For analysis of osteogenic differentiation potential, BM-MSCs were incubated in osteogenic differentiation medium for 2 weeks. BM-MSCs that differentiated into osteoblasts were identified by alizarin red staining (ARS), which stains calcium deposits in the differentiated cells, and quantified by measuring the absorbance at 550 nm. There was no significant difference in osteogenic differentiation potential between BM-MSCs cultured with and without 1% (wt/v) gelatin. (d) For analysis of neurogenic differentiation potential, BM-MSCs were cultured for 7 days in neurogenic differentiation medium. BM-MSCs that differentiated into neurogenic cells were stained with a fluorescence-conjugated primary antibody against Nestin (neural lineage marker) and assessed by flow cytometry. A significantly higher percentage of Nestin-positive cells was detected in BM-MSCs cultured on 1% (wt/v) gelatin matrix than in those cultured on plates without gelatin. All data shown are means ± S.D. of three (d) or four ((b) and (c)) independent experiments. * P

    Article Snippet: Chondrogenic Differentiation of BM-MSCs Differentiation of BM-MSCs into chondrocytes was conducted using a StemPro Chondrogenesis Differentiation Kit (Gibco Invitrogen), according to the manufacturer's instructions.

    Techniques: Cell Culture, Staining, Incubation, Fluorescence, Marker, Flow Cytometry, Cytometry

    Gene expression analysis for fold-change of chondrogenic markers in physioxia relative to hyperoxia demonstrates that high-GAG groups of both MSCs and ACPs are highly responsive to oxygen level and upregulate a majority of genes representative of the articular cartilage phenotype in low-oxygen environments. Data are mean ± standard deviation of fold-change in gene expression for each group ( n = 8). Statistical significance defined as * p

    Journal: Stem Cell Research & Therapy

    Article Title: Responses to altered oxygen tension are distinct between human stem cells of high and low chondrogenic capacity

    doi: 10.1186/s13287-016-0419-8

    Figure Lengend Snippet: Gene expression analysis for fold-change of chondrogenic markers in physioxia relative to hyperoxia demonstrates that high-GAG groups of both MSCs and ACPs are highly responsive to oxygen level and upregulate a majority of genes representative of the articular cartilage phenotype in low-oxygen environments. Data are mean ± standard deviation of fold-change in gene expression for each group ( n = 8). Statistical significance defined as * p

    Article Snippet: Pellet cultures were formed by centrifuging 5 × 104 cells (MSCs) or 1 × 105 cells (ACPs) at 500 × g for 5 min in 240 μl of medium in Nunc polypropylene V-bottom 96-well plates (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Expressing, Standard Deviation

    a Representative toluidine blue stain for total proteoglycans demonstrates smaller pellets with less metachromasia for low-GAG MSC preparations and ACP clones in both hyperoxia and physioxia relative to paired high-GAG MSC preparations and ACP clones at the respective oxygen levels. Images were acquired with bright-field microscopy, scale bars = 100 μm. b Measurement of pellet diameter revealed a statistically significant difference in pellet size between both MSCs and ACPs of high or low chondrogenicity and between high-GAG MSCs at physioxia or hyperoxia. Statistical significance defined as * p

    Journal: Stem Cell Research & Therapy

    Article Title: Responses to altered oxygen tension are distinct between human stem cells of high and low chondrogenic capacity

    doi: 10.1186/s13287-016-0419-8

    Figure Lengend Snippet: a Representative toluidine blue stain for total proteoglycans demonstrates smaller pellets with less metachromasia for low-GAG MSC preparations and ACP clones in both hyperoxia and physioxia relative to paired high-GAG MSC preparations and ACP clones at the respective oxygen levels. Images were acquired with bright-field microscopy, scale bars = 100 μm. b Measurement of pellet diameter revealed a statistically significant difference in pellet size between both MSCs and ACPs of high or low chondrogenicity and between high-GAG MSCs at physioxia or hyperoxia. Statistical significance defined as * p

    Article Snippet: Pellet cultures were formed by centrifuging 5 × 104 cells (MSCs) or 1 × 105 cells (ACPs) at 500 × g for 5 min in 240 μl of medium in Nunc polypropylene V-bottom 96-well plates (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Staining, Clone Assay, Microscopy

    Clustering of oxygen-dependent gene expression based on z -score demonstrates that groups of MSCs and ACPs are more similar between GAG level than within cell type in response to culture in physioxia relative to culture in hyperoxia. ACP articular cartilage progenitor, GAG glycosaminoglycan, MSC mesenchymal stem cell

    Journal: Stem Cell Research & Therapy

    Article Title: Responses to altered oxygen tension are distinct between human stem cells of high and low chondrogenic capacity

    doi: 10.1186/s13287-016-0419-8

    Figure Lengend Snippet: Clustering of oxygen-dependent gene expression based on z -score demonstrates that groups of MSCs and ACPs are more similar between GAG level than within cell type in response to culture in physioxia relative to culture in hyperoxia. ACP articular cartilage progenitor, GAG glycosaminoglycan, MSC mesenchymal stem cell

    Article Snippet: Pellet cultures were formed by centrifuging 5 × 104 cells (MSCs) or 1 × 105 cells (ACPs) at 500 × g for 5 min in 240 μl of medium in Nunc polypropylene V-bottom 96-well plates (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Expressing

    Gene expression analysis for fold-change of chondrogenic markers of the articular cartilage phenotype ( COL2A1 , ACAN ), the fibrocartilaginous phenotype ( COL1A1 ), and the hypertrophic phenotype ( COL10A1 , MMP13 ) demonstrates varied chondrogenic responses by high-GAG and low-GAG groups of each cell type, MSCs and ACPs, during pellet culture in physioxic relative to hyperoxic conditions. Data are mean ± standard deviation of fold-change for each group ( n = 6–10). Statistical significance defined as * p

    Journal: Stem Cell Research & Therapy

    Article Title: Responses to altered oxygen tension are distinct between human stem cells of high and low chondrogenic capacity

    doi: 10.1186/s13287-016-0419-8

    Figure Lengend Snippet: Gene expression analysis for fold-change of chondrogenic markers of the articular cartilage phenotype ( COL2A1 , ACAN ), the fibrocartilaginous phenotype ( COL1A1 ), and the hypertrophic phenotype ( COL10A1 , MMP13 ) demonstrates varied chondrogenic responses by high-GAG and low-GAG groups of each cell type, MSCs and ACPs, during pellet culture in physioxic relative to hyperoxic conditions. Data are mean ± standard deviation of fold-change for each group ( n = 6–10). Statistical significance defined as * p

    Article Snippet: Pellet cultures were formed by centrifuging 5 × 104 cells (MSCs) or 1 × 105 cells (ACPs) at 500 × g for 5 min in 240 μl of medium in Nunc polypropylene V-bottom 96-well plates (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Expressing, Standard Deviation

    Differential gene expression by Ad-MSC versus BM-MSC. The Partek Genomics Suite was used to import microarray data from the canine 1.0 ST Affymetrix chip. (A) A principle component analysis plot generated from these data depicts three biological replicates of each cell type, with red representing Ad-MSC, and blue representing BM-MSC. An ellipsoid was drawn around cell types using a sample standard deviation of 2. PC1 depicts a variation of 36.3%, PC2 a variation of 22.4%, and PC3 a variation of 5.6%. (B) Volcano plot of all differentially expressed genes in the analysis of Ad-MSC versus BM-MSC. x-Axis represents fold change, thick axis lines drawn at fold change −2 and 2. y-Axis represents P value, thick axis line marks 0.02. Color code represents column number assigned to each gene location on the Affymetrix chip. (C) section. Red represents a higher expression, and blue represents lower expression with scale bar for each category (Ad-MSC or BM-MSC) on the left side of plots.

    Journal: Stem Cells and Development

    Article Title: Mechanisms of Immune Suppression Utilized by Canine Adipose and Bone Marrow-Derived Mesenchymal Stem Cells

    doi: 10.1089/scd.2016.0207

    Figure Lengend Snippet: Differential gene expression by Ad-MSC versus BM-MSC. The Partek Genomics Suite was used to import microarray data from the canine 1.0 ST Affymetrix chip. (A) A principle component analysis plot generated from these data depicts three biological replicates of each cell type, with red representing Ad-MSC, and blue representing BM-MSC. An ellipsoid was drawn around cell types using a sample standard deviation of 2. PC1 depicts a variation of 36.3%, PC2 a variation of 22.4%, and PC3 a variation of 5.6%. (B) Volcano plot of all differentially expressed genes in the analysis of Ad-MSC versus BM-MSC. x-Axis represents fold change, thick axis lines drawn at fold change −2 and 2. y-Axis represents P value, thick axis line marks 0.02. Color code represents column number assigned to each gene location on the Affymetrix chip. (C) section. Red represents a higher expression, and blue represents lower expression with scale bar for each category (Ad-MSC or BM-MSC) on the left side of plots.

    Article Snippet: To compare the gene expression patterns of Ad-MSC and BM-MSC, microarray studies were done, using Affymetrix canine OST 1.0 chips. (The full array data have been deposited in the Gene Expression Omnibus database online.)

    Techniques: Expressing, Microarray, Chromatin Immunoprecipitation, Generated, Standard Deviation