Journal: NAR Cancer

Article Title: Targeted CRISPR knockout screening identifies known and novel chemogenomic interactions between DNA damaging agents and DNA repair genes

doi: 10.1093/narcan/zcaf052

Figure Lengend Snippet: Knockout of genes involved in TC-NER confers sensitivity to monofunctional alkylating agents. ( A and B ) MAGeCK-RRA results from illudin S and aflatoxin B1 screens. ( C ) Western blot demonstrating CRISPR–Cas9-mediated KO of ERCC8 in LN229 and SW620 cells. ( D and E ) Bar graph representing IC 50 values obtained of WT or ERCC8 KO LN229 and SW620 cells treated with (D) illudin S or (E) aflatoxin B1 for 5 days. Bars, geometric mean; error bars, ⋇ geometric SD, n = 3–4, ratio paired t -test (one-tailed) ** P < 0.01, *** P < 0.001.

Article Snippet: Cas9-expressing LN229 and SW620 cells were authenticated by either IDEXX Bioanalytics (Cell Check 16) or the Yale Keck DNA Sequencing Facility.

Techniques: Knock-Out, Western Blot, CRISPR, One-tailed Test

Journal: NAR Cancer

Article Title: Targeted CRISPR knockout screening identifies known and novel chemogenomic interactions between DNA damaging agents and DNA repair genes

doi: 10.1093/narcan/zcaf052

Figure Lengend Snippet: Knockout of genes involved in FA confers sensitivity to bifunctional and methylating agents. ( A–D ) MAGeCK-RRA results from bifunctional alkylating agents (A) cisplatin and (B) mitomycin C as well as methylating agents (C) TMZ and (D) MMS. ( E ) Western blot demonstrating CRISPR–Cas9-mediated knockout of FANCA and FANCD2 in LN229 and SW620 cells. ( F and G ) Bar graph representing IC 50 values from WT, FANCA KO, and FANCD2 KO LN229 and SW620 cells treated with (F) TMZ or (G) MMS for 5 days. Bars, geometric mean; error bars, ⋇ geometric SD, n = 3–4, ratio paired t -test (one-tailed) ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Cas9-expressing LN229 and SW620 cells were authenticated by either IDEXX Bioanalytics (Cell Check 16) or the Yale Keck DNA Sequencing Facility.

Techniques: Knock-Out, Western Blot, CRISPR, One-tailed Test

Journal: NAR Cancer

Article Title: Targeted CRISPR knockout screening identifies known and novel chemogenomic interactions between DNA damaging agents and DNA repair genes

doi: 10.1093/narcan/zcaf052

Figure Lengend Snippet: Topoisomerase poisons topotecan and etoposide exhibit differential genetic dependencies. ( A and B ) MAGeCK-RRA results from topoisomerase poisons (A) topotecan and (B) etoposide. ( C ) Western blot demonstrating CRISPR–Cas9-mediated knockout of NHEJ1 in LN229 and SW620 cells. ( D and E ) Bar graph representing IC 50 values in WT and NHEJ1 KO LN229 (clone #7) and SW620 cells treated with (D) etoposide or (E) topotecan for 5 days. Bars, geometric mean; error bars, ⋇ geometric SD, n = 3, ratio paired t -test (one-tailed for etoposide, two-tailed for topotecan) * P < 0.05, *** P < 0.001, n.s., not significant.

Article Snippet: Cas9-expressing LN229 and SW620 cells were authenticated by either IDEXX Bioanalytics (Cell Check 16) or the Yale Keck DNA Sequencing Facility.

Techniques: Western Blot, CRISPR, Knock-Out, One-tailed Test, Two Tailed Test