cell culture flasks  (Thermo Fisher)


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  • 99
    Name:
    Nunc Cell Culture Treated Flasks
    Description:
    Achieve maximum adhesion for a broad range of cell types with Thermo Scientific Nunc Cell Culture Treated Flasks with Filter Caps These flasks receive the proprietary Nunclon Delta surface treatment to ensure consistent cell growth The filter caps allow constant airflow and minimize chances of contamination
    Catalog Number:
    136196
    Price:
    None
    Applications:
    Cell Culture|Cell Culture Plastics|Laboratory Plastics and Supplies|Microplates, Dishes and Flasks
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    Lab Supplies Plastics Glassware
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    Structured Review

    Thermo Fisher cell culture flasks
    Achieve maximum adhesion for a broad range of cell types with Thermo Scientific Nunc Cell Culture Treated Flasks with Filter Caps These flasks receive the proprietary Nunclon Delta surface treatment to ensure consistent cell growth The filter caps allow constant airflow and minimize chances of contamination
    https://www.bioz.com/result/cell culture flasks/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cell culture flasks - by Bioz Stars, 2020-09
    99/100 stars

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    Cell Culture:

    Article Title: Mitochondrial damage by α-synuclein causes cell death in human dopaminergic neurons
    Article Snippet: .. LUHMES cells were proliferated in cell culture flasks (Nunclon DELTA surface, NUNC A/S, St. Louis, MO, USA) coated with 0.1 mg/ml poly-l -lysine (PLL) (Sigma-Aldrich, St. Louis MO, USA) at +4 °C overnight. .. For experiments, cell culture dishes were coated with 0.1 mg/ml PLL overnight and washed three times with sterile water, followed by coating with 5 µg/ml fibronectin (Sigma-Aldrich, St. Louis MO, USA) overnight in the incubator (37 °C, 5% CO2 ).

    Article Title: Acarbose Protects From Central and Peripheral Metabolic Imbalance Induced by Benzene Exposure
    Article Snippet: .. After homogenization, the cells were grown in Nunc™ Cell Culture Treated Flasks with Filter Caps, (Thermo Fischer, 178905) containing 10 mL of the medium [DMEM/F-12; 10% FBS; 1% Antibiotic-Antimycotic] in a 5% CO2 incubator (Galaxy 170R, Eppendorf) at 37°C. .. The cells were maintained in culture for a period of 8–10 days, with a partial replacement of the incubation medium (70%) every 48–72h.

    Article Title: Functional arrays of human pluripotent stem cell-derived cardiac microtissues
    Article Snippet: .. Fibroblasts were cultured in T175 flasks (Nalgene) for up to 10 passages before being discarded. .. Seeding and cultivation of CaMiRi hPSC-CM were suspended in a collagen mastermix and seeded into cardiac microtissue wells at desired density (optimal condition determined was 90% cardiomyocytes, a collagen concentration of 2.0 mg/mL, and a total cell concentration of 75,000 per microtissue).

    Article Title: The lysine deacetylase inhibitor givinostat inhibits ?-cell IL-1? induced IL-1? transcription and processing
    Article Snippet: .. Cells were maintained in RPMI 1640 medium with glutamax (GIBCO, 153732), supplemented with 10% (heat-inactivated) fetal calf serum (GIBCO, 26140-079), 100 U/mL penicillin, 100 μg/mL streptomycin (GIBCO, 15140-122) and 50 μM β-mercaptoethanol (Sigma, M7522) and cultured at 37°C in a humidified atmosphere containing 5% CO2 . ..

    other:

    Article Title: RETrace: simultaneous retrospective lineage tracing and methylation profiling of single cells
    Article Snippet: The goal of single-cell culture was to create a phylogenetic tree with known structure.

    Microscopy:

    Article Title: Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome.
    Article Snippet: .. Despite decades of research, little is known about the cellular targets and the mode of action of the vast majority of antimalarial drugs. ..

    Magnetic Cell Separation:

    Article Title: Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome.
    Article Snippet: .. Despite decades of research, little is known about the cellular targets and the mode of action of the vast majority of antimalarial drugs. ..

    Homogenization:

    Article Title: Acarbose Protects From Central and Peripheral Metabolic Imbalance Induced by Benzene Exposure
    Article Snippet: .. After homogenization, the cells were grown in Nunc™ Cell Culture Treated Flasks with Filter Caps, (Thermo Fischer, 178905) containing 10 mL of the medium [DMEM/F-12; 10% FBS; 1% Antibiotic-Antimycotic] in a 5% CO2 incubator (Galaxy 170R, Eppendorf) at 37°C. .. The cells were maintained in culture for a period of 8–10 days, with a partial replacement of the incubation medium (70%) every 48–72h.

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  • 99
    Thermo Fisher nunclon δ surface t25 cm2 tissue culture flasks
    Time-course of IL-12p70 production by DCs . IL-12p70 was detected from fresh DCs from 6 h post LPS and IFN-γ stimulation and peaked at 16 h. Fresh replated DCs and thawed replated DCs continued to produce IL-12p70 albeit at a lower level than fresh DCs. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in duplicates in research-scale <t>Nunclon™Δ</t> Surface <t>T25</t> cm 2 flasks ± SEM. IL-12p70 concentration is expressed as pg/ml.
    Nunclon δ Surface T25 Cm2 Tissue Culture Flasks, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunclon δ surface t25 cm2 tissue culture flasks/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    nunclon δ surface t25 cm2 tissue culture flasks - by Bioz Stars, 2020-09
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    95
    Thermo Fisher biolite cell culture treated flasks
    Restrictively infected astrocytes were resistant to reactivation. Human astrocytes were infected with R/G-HIV-1-WT in T75 tissue <t>culture</t> <t>flasks.</t> At 2 weeks postinfection <t>cells</t> were <t>treated</t> with either IL-1β (20 ng/ml) or vorinostat (1 μM) alone or in combination for 24 h. (A, B, C, and D) Cells were harvested, and fluorescence reporter activity was measured by flow analysis. Histograms are representative of seven donors. (E) Quantitative flow data were normalized to the total untreated R/G-HIV-1-WT population. (F) In parallel, HIV-1 Tat levels were evaluated by real-time PCR. The graph shows cumulative results of three experiments with individual astrocyte donors. (G) Supernatants were collected at 24 h posttreatment and assayed for HIV-1 viral protein p24. The graph shows cumulative results for seven individual astrocyte donors (*, P
    Biolite Cell Culture Treated Flasks, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biolite cell culture treated flasks/product/Thermo Fisher
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biolite cell culture treated flasks - by Bioz Stars, 2020-09
    95/100 stars
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    99
    Thermo Fisher v bottom 96
    Dispersion and reaggregation of islet cells reduces heterogeneity in glucose-stimulated respiration among individual islets . (A) Dispersion and reaggregation of islets. Isolated mouse islets were cultured overnight, pooled and dispersed with accutase into single cells. Single islet cells were seeded at 6,000–10,000 cells/well and cultured for 48–72 h in V-bottom 96 well plates to facilitate reaggregation into islet structures (reaggregated islets). (B) Glucose-stimulated respiration of individual intact or reaggregated human islets, normalized to basal OCR (% Basal). Intact or reaggregated human islets were derived from the same donors. n = 5 intact islets and 7 reaggregated islets from 2 donors. (C) Glucose-stimulated respiration of individual intact or reaggregated mouse islets, normalized to basal OCR (% Basal). Intact and reaggregated islets were derived from the same islet preparation. n = 8 intact and 7 reaggregated islets from one islet isolation. Coefficient of variation (CV) was calculated as an estimate of variability. Human islets in (B) were obtained from deceased donors from University of Alberta Diabetes Institute Islet Core. Data in (B–C) are means ± SD.
    V Bottom 96, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/v bottom 96/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    v bottom 96 - by Bioz Stars, 2020-09
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    Time-course of IL-12p70 production by DCs . IL-12p70 was detected from fresh DCs from 6 h post LPS and IFN-γ stimulation and peaked at 16 h. Fresh replated DCs and thawed replated DCs continued to produce IL-12p70 albeit at a lower level than fresh DCs. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in duplicates in research-scale Nunclon™Δ Surface T25 cm 2 flasks ± SEM. IL-12p70 concentration is expressed as pg/ml.

    Journal: Journal of Translational Medicine

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    doi: 10.1186/1479-5876-9-198

    Figure Lengend Snippet: Time-course of IL-12p70 production by DCs . IL-12p70 was detected from fresh DCs from 6 h post LPS and IFN-γ stimulation and peaked at 16 h. Fresh replated DCs and thawed replated DCs continued to produce IL-12p70 albeit at a lower level than fresh DCs. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in duplicates in research-scale Nunclon™Δ Surface T25 cm 2 flasks ± SEM. IL-12p70 concentration is expressed as pg/ml.

    Article Snippet: Nunclon™Δ Surface T25 cm2 tissue culture flasks with filter caps and Nunclon™Δ Surface 1-tray Cell Factories were purchased from Thermo Fisher Scientific Inc., Rochester, NY, USA.

    Techniques: Concentration Assay

    Mixed leukocyte reaction capability of optimized DCs . DCs generated from clinical-scale Nunclon™Δ Surface 1-tray Cell Factory and DCs generated from research-scale Nunclon™Δ Surface T25 cm 2 flasks using the optimized protocol depicted in Figure 6 were equally potent in stimulating robust mixed leukocytes reactions from 3 different individuals' T cells.

    Journal: Journal of Translational Medicine

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    doi: 10.1186/1479-5876-9-198

    Figure Lengend Snippet: Mixed leukocyte reaction capability of optimized DCs . DCs generated from clinical-scale Nunclon™Δ Surface 1-tray Cell Factory and DCs generated from research-scale Nunclon™Δ Surface T25 cm 2 flasks using the optimized protocol depicted in Figure 6 were equally potent in stimulating robust mixed leukocytes reactions from 3 different individuals' T cells.

    Article Snippet: Nunclon™Δ Surface T25 cm2 tissue culture flasks with filter caps and Nunclon™Δ Surface 1-tray Cell Factories were purchased from Thermo Fisher Scientific Inc., Rochester, NY, USA.

    Techniques: Generated

    Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® CellSTACK ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.

    Journal: Journal of Translational Medicine

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    doi: 10.1186/1479-5876-9-198

    Figure Lengend Snippet: Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® CellSTACK ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.

    Article Snippet: Nunclon™Δ Surface T25 cm2 tissue culture flasks with filter caps and Nunclon™Δ Surface 1-tray Cell Factories were purchased from Thermo Fisher Scientific Inc., Rochester, NY, USA.

    Techniques: Cell Culture

    Comparison of TrypLE™ Select animal-free tyrpsin immobilization and cold PBS with cell scraping for harvesting mature HOCl-oxidized lysate-loaded DCs . (A) DCs were matured with LPS and IFN-γ for 16 h following lysate-loading. It was observed that mature lysate-loaded DCs that were harvested using TrypLE™ Select animal-free tyrpsin replacement exhibited approximately 30-40% reduction in the levels of MHC Class I ( P = 0.13; NS) and HLA-DR ( P = 0.15; NS) compared to DCs that were harvested with cold DPBS and cell scraping. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) ± SEM in research-scale Nunclon™Δ Surface T25 cm 2 flasks. P values were determined with unpaired Student's t test. NS indicated that P values were not significant. (B) Mature lysate-loaded DCs that were harvested with TrypLE™ Select animal-free tyrpsin replacement were as efficient as DCs that were harvested with cold DPBS and cell scraping in presenting MHC Class I-restricted HER-2/neu and MART-1 peptides and stimulating HER-2/neu and MART-1 CD8 + T cells in intracellular IFN-γ assessments.

    Journal: Journal of Translational Medicine

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    doi: 10.1186/1479-5876-9-198

    Figure Lengend Snippet: Comparison of TrypLE™ Select animal-free tyrpsin immobilization and cold PBS with cell scraping for harvesting mature HOCl-oxidized lysate-loaded DCs . (A) DCs were matured with LPS and IFN-γ for 16 h following lysate-loading. It was observed that mature lysate-loaded DCs that were harvested using TrypLE™ Select animal-free tyrpsin replacement exhibited approximately 30-40% reduction in the levels of MHC Class I ( P = 0.13; NS) and HLA-DR ( P = 0.15; NS) compared to DCs that were harvested with cold DPBS and cell scraping. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) ± SEM in research-scale Nunclon™Δ Surface T25 cm 2 flasks. P values were determined with unpaired Student's t test. NS indicated that P values were not significant. (B) Mature lysate-loaded DCs that were harvested with TrypLE™ Select animal-free tyrpsin replacement were as efficient as DCs that were harvested with cold DPBS and cell scraping in presenting MHC Class I-restricted HER-2/neu and MART-1 peptides and stimulating HER-2/neu and MART-1 CD8 + T cells in intracellular IFN-γ assessments.

    Article Snippet: Nunclon™Δ Surface T25 cm2 tissue culture flasks with filter caps and Nunclon™Δ Surface 1-tray Cell Factories were purchased from Thermo Fisher Scientific Inc., Rochester, NY, USA.

    Techniques:

    Comparing the phenotype of DCs previously cryopreserved in infusible cryomedia or human AB serum containing 10% DMSO . (A) Middle panel, DCs that had been cyropreserved with infusible cryomedia showed similar expressions of CD86 and CD1c as fresh DCs (first panel) and higher CD80, CD40 and MHC Class I levels after being thawed and replated for 24 h in fresh human AB serum-supplemented CellGenix DC media. Third panel, DCs that were cryopreserved with 90% human AB serum plus 10% DMSO exhibited similar level of CD80, CD40, MHC Class I and CD1c as fresh DCs. DCs cryopreserved in infusible cryomedia expressed approximately 50% lower levels of HLA-DR (** P = 0.001) compared to fresh DCs. On the other hand, DCs cryopreserved in human AB serum plus 10% DMSO media expressed approximately 80% lower levels of HLA-DR (** P = 0.004) compared to fresh DCs. Data were from a clinical-scale Nunclon™Δ Surface 1-tray Cell Factory and was representative of 3 independent research-scales experiments (i.e. DCs from 3 different individuals) in Nunclon™Δ Surface T25 cm 2 flasks. (B) Summary of flow cytometry results from the mean of 3 independent research-scales experiments in Nunclon™Δ Surface T25 cm 2 flasks (MFI ± SEM). P values are determined with unpaired Student's t test. ** denotes P value highly significant.

    Journal: Journal of Translational Medicine

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    doi: 10.1186/1479-5876-9-198

    Figure Lengend Snippet: Comparing the phenotype of DCs previously cryopreserved in infusible cryomedia or human AB serum containing 10% DMSO . (A) Middle panel, DCs that had been cyropreserved with infusible cryomedia showed similar expressions of CD86 and CD1c as fresh DCs (first panel) and higher CD80, CD40 and MHC Class I levels after being thawed and replated for 24 h in fresh human AB serum-supplemented CellGenix DC media. Third panel, DCs that were cryopreserved with 90% human AB serum plus 10% DMSO exhibited similar level of CD80, CD40, MHC Class I and CD1c as fresh DCs. DCs cryopreserved in infusible cryomedia expressed approximately 50% lower levels of HLA-DR (** P = 0.001) compared to fresh DCs. On the other hand, DCs cryopreserved in human AB serum plus 10% DMSO media expressed approximately 80% lower levels of HLA-DR (** P = 0.004) compared to fresh DCs. Data were from a clinical-scale Nunclon™Δ Surface 1-tray Cell Factory and was representative of 3 independent research-scales experiments (i.e. DCs from 3 different individuals) in Nunclon™Δ Surface T25 cm 2 flasks. (B) Summary of flow cytometry results from the mean of 3 independent research-scales experiments in Nunclon™Δ Surface T25 cm 2 flasks (MFI ± SEM). P values are determined with unpaired Student's t test. ** denotes P value highly significant.

    Article Snippet: Nunclon™Δ Surface T25 cm2 tissue culture flasks with filter caps and Nunclon™Δ Surface 1-tray Cell Factories were purchased from Thermo Fisher Scientific Inc., Rochester, NY, USA.

    Techniques: Flow Cytometry, Cytometry

    Comparison of the phenotype and IL-12p70 production of mature HOCl-oxidized lysate-loaded DCs generated in human AB serum-free or serum-containing AIM-V and CellGenix DC media, and supplementation with different concentrations of GM-CSF and IL-4 . (A-B) DCs cultured in CellGenix DC media containing 2% human AB serum displayed a desirable phenotype with the highest level of DC-LAMP (* P = 0.005; one-way ANOVA) when compared to DCs cultured in other media conditions, and significantly higher CD86 (* P = 0.05) and lower CD14 (* P = 0.04) when compared to DCs cultured in serum-free CellGenix DC media. (C) DCs generated in CellGenix DC media plus 2% human AB serum produced slightly, though not significantly, higher amount of IL-12p70 compared to DCs generated in other media conditions ( P = 0.2; one-way ANOVA). (D) 500 IU/ml recombinant GM-CSF and 250 IU/ml recombinant IL-4 were sufficient concentrations for generating a favorable immature DC phenotype with low CD14, and intermediate HLA-DR and CD1c. All the data presented were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures in Nunclon™Δ Surface T25 cm 2 flasks ± standard error of the mean (SEM). P values were determined with unpaired Student's t test unless otherwise stated. * denoted that P values were significant.

    Journal: Journal of Translational Medicine

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    doi: 10.1186/1479-5876-9-198

    Figure Lengend Snippet: Comparison of the phenotype and IL-12p70 production of mature HOCl-oxidized lysate-loaded DCs generated in human AB serum-free or serum-containing AIM-V and CellGenix DC media, and supplementation with different concentrations of GM-CSF and IL-4 . (A-B) DCs cultured in CellGenix DC media containing 2% human AB serum displayed a desirable phenotype with the highest level of DC-LAMP (* P = 0.005; one-way ANOVA) when compared to DCs cultured in other media conditions, and significantly higher CD86 (* P = 0.05) and lower CD14 (* P = 0.04) when compared to DCs cultured in serum-free CellGenix DC media. (C) DCs generated in CellGenix DC media plus 2% human AB serum produced slightly, though not significantly, higher amount of IL-12p70 compared to DCs generated in other media conditions ( P = 0.2; one-way ANOVA). (D) 500 IU/ml recombinant GM-CSF and 250 IU/ml recombinant IL-4 were sufficient concentrations for generating a favorable immature DC phenotype with low CD14, and intermediate HLA-DR and CD1c. All the data presented were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures in Nunclon™Δ Surface T25 cm 2 flasks ± standard error of the mean (SEM). P values were determined with unpaired Student's t test unless otherwise stated. * denoted that P values were significant.

    Article Snippet: Nunclon™Δ Surface T25 cm2 tissue culture flasks with filter caps and Nunclon™Δ Surface 1-tray Cell Factories were purchased from Thermo Fisher Scientific Inc., Rochester, NY, USA.

    Techniques: Generated, Cell Culture, Produced, Recombinant

    Restrictively infected astrocytes were resistant to reactivation. Human astrocytes were infected with R/G-HIV-1-WT in T75 tissue culture flasks. At 2 weeks postinfection cells were treated with either IL-1β (20 ng/ml) or vorinostat (1 μM) alone or in combination for 24 h. (A, B, C, and D) Cells were harvested, and fluorescence reporter activity was measured by flow analysis. Histograms are representative of seven donors. (E) Quantitative flow data were normalized to the total untreated R/G-HIV-1-WT population. (F) In parallel, HIV-1 Tat levels were evaluated by real-time PCR. The graph shows cumulative results of three experiments with individual astrocyte donors. (G) Supernatants were collected at 24 h posttreatment and assayed for HIV-1 viral protein p24. The graph shows cumulative results for seven individual astrocyte donors (*, P

    Journal: Journal of Virology

    Article Title: Insights into the Gene Expression Profiles of Active and Restricted Red/Green-HIV+ Human Astrocytes: Implications for Shock or Lock Therapies in the Brain

    doi: 10.1128/JVI.01563-19

    Figure Lengend Snippet: Restrictively infected astrocytes were resistant to reactivation. Human astrocytes were infected with R/G-HIV-1-WT in T75 tissue culture flasks. At 2 weeks postinfection cells were treated with either IL-1β (20 ng/ml) or vorinostat (1 μM) alone or in combination for 24 h. (A, B, C, and D) Cells were harvested, and fluorescence reporter activity was measured by flow analysis. Histograms are representative of seven donors. (E) Quantitative flow data were normalized to the total untreated R/G-HIV-1-WT population. (F) In parallel, HIV-1 Tat levels were evaluated by real-time PCR. The graph shows cumulative results of three experiments with individual astrocyte donors. (G) Supernatants were collected at 24 h posttreatment and assayed for HIV-1 viral protein p24. The graph shows cumulative results for seven individual astrocyte donors (*, P

    Article Snippet: Primary human astrocytes were seeded in either T75 tissue culture flasks (10 × 106 cells/flask) (catalog no. 130190; Thermo Fisher, Waltham, MA) or six-well plates (2 × 106 cells/well) and allowed to recover overnight.

    Techniques: Infection, Fluorescence, Activity Assay, Real-time Polymerase Chain Reaction

    Dispersion and reaggregation of islet cells reduces heterogeneity in glucose-stimulated respiration among individual islets . (A) Dispersion and reaggregation of islets. Isolated mouse islets were cultured overnight, pooled and dispersed with accutase into single cells. Single islet cells were seeded at 6,000–10,000 cells/well and cultured for 48–72 h in V-bottom 96 well plates to facilitate reaggregation into islet structures (reaggregated islets). (B) Glucose-stimulated respiration of individual intact or reaggregated human islets, normalized to basal OCR (% Basal). Intact or reaggregated human islets were derived from the same donors. n = 5 intact islets and 7 reaggregated islets from 2 donors. (C) Glucose-stimulated respiration of individual intact or reaggregated mouse islets, normalized to basal OCR (% Basal). Intact and reaggregated islets were derived from the same islet preparation. n = 8 intact and 7 reaggregated islets from one islet isolation. Coefficient of variation (CV) was calculated as an estimate of variability. Human islets in (B) were obtained from deceased donors from University of Alberta Diabetes Institute Islet Core. Data in (B–C) are means ± SD.

    Journal: Molecular Metabolism

    Article Title: Individual islet respirometry reveals functional diversity within the islet population of mice and human donors

    doi: 10.1016/j.molmet.2018.07.003

    Figure Lengend Snippet: Dispersion and reaggregation of islet cells reduces heterogeneity in glucose-stimulated respiration among individual islets . (A) Dispersion and reaggregation of islets. Isolated mouse islets were cultured overnight, pooled and dispersed with accutase into single cells. Single islet cells were seeded at 6,000–10,000 cells/well and cultured for 48–72 h in V-bottom 96 well plates to facilitate reaggregation into islet structures (reaggregated islets). (B) Glucose-stimulated respiration of individual intact or reaggregated human islets, normalized to basal OCR (% Basal). Intact or reaggregated human islets were derived from the same donors. n = 5 intact islets and 7 reaggregated islets from 2 donors. (C) Glucose-stimulated respiration of individual intact or reaggregated mouse islets, normalized to basal OCR (% Basal). Intact and reaggregated islets were derived from the same islet preparation. n = 8 intact and 7 reaggregated islets from one islet isolation. Coefficient of variation (CV) was calculated as an estimate of variability. Human islets in (B) were obtained from deceased donors from University of Alberta Diabetes Institute Islet Core. Data in (B–C) are means ± SD.

    Article Snippet: Cells were counted and seeded at 6,000, 8,000 or 10,000 cells per well of a V-bottom 96 well plate (Thermo Fisher Scientific, Roskilde, Denmark).

    Techniques: Isolation, Cell Culture, Derivative Assay