cell activation cocktail  (BioLegend)

 
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    Name:
    Cell Activation Cocktail with Brefeldin A
    Description:
    Cell Activation Cocktail with Brefeldin A Apps Activ FC ICFC Size 100 μl
    Catalog Number:
    423303
    Price:
    95
    Applications:
    Activ, FC, ICFC
    Size:
    100 μl
    Category:
    Buffer Solution Chemical
    Preparation:
    Cell Activation Cocktail with Brefeldin A is composed of PMA ionomycin and Brefeldin A
    Quantity:
    1
    Buy from Supplier


    Structured Review

    BioLegend cell activation cocktail
    Cell Activation Cocktail with Brefeldin A
    Cell Activation Cocktail with Brefeldin A Apps Activ FC ICFC Size 100 μl
    https://www.bioz.com/result/cell activation cocktail/product/BioLegend
    Average 99 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    cell activation cocktail - by Bioz Stars, 2020-11
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Transcriptional profiling and therapeutic targeting of oxidative stress in neuroinflammation.
    Article Snippet: .. Oxidative stress is a central part of innate immune-induced neurodegeneration. .. Oxidative stress is a central part of innate immune-induced neurodegeneration.

    Article Title: CD147 blockade as a potential and novel treatment of graft rejection
    Article Snippet: .. Fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD4 and phycoerythrin (PE)-conjugated mouse anti-human CD3 antibodies, Foxp3 staining buffer and a cell activation cocktail used for intracellular staining were purchased from BioLegend. .. PE-Cy7-conjugated rat anti-mouse IFN-γ, PE-conjugated rat anti-mouse IL-4, Alexa 647-conjugated rat anti-mouse IL-17, FITC-conjugated rat anti-mouse CD4, allophycocynanin-H7-conjugated rat anti-mouse CD8, PE-Cy7-conjugated rat anti-mouse CD44, BUV737-conjugated rat anti-mouse CD62 L, allophycocyanin-conjugated rat anti-mouse CD25, and PE-conjugated rat anti-mouse Foxp3 antibodies were purchased from BD Biosciences (San Diego, CA, USA).

    Article Title: MUC1-induced immunosuppression in colon cancer can be reversed by blocking the PD1/PDL1 signaling pathway
    Article Snippet: .. For the intracellular staining of immune cells, 1×106 cells/ml were treated with Cell Activation Cocktail containing Brefeldin A (1:1,000; cat. no. 423304; BioLegend, Inc.). .. After 4 h, the cells were collected and stained with the aforementioned Brilliant Violet 510™- or FITC-conjugated mAbs against mouse or human CD45, CD4 or CD8 mAbs (BioLegend, Inc) at 4°C for 30 min.

    Article Title: Microwave ablation combined with OK-432 induces Th1-type response and specific antitumor immunity in a murine model of breast cancer
    Article Snippet: .. For intracellular cytokine staining, harvested cells were stimulated with phorbol myristate acetate (PMA), ionomycin, and brefeldin A (Cell Activation Cocktail, Biolegend, San Diego, USA) for 5 h. FITC-anti-CD4 (RM4-5), APC/Cy7-anti-CD8a (53-6.7), and matched isotype control antibodies were purchased from BD Biosciences. .. PerCP/Cy5.5-anti-CD4 (RM4-5), FITC-anti-IFN-γ (XMG1.2), APC-anti- interleukin (IL)-4 (11B11), and matched isotype control antibodies were purchased from Biolegend.

    Article Title: Decidual RANKL/RANK interaction promotes the residence and polarization of TGF-β1-producing regulatory γδ T cells
    Article Snippet: .. Intracellular cytokines were detected after stimulating with Cell Activation Cocktail (Biolegend) for 6 h. Intracellular proteins or intranuclear transcription factors were stained using Fixation/Permeabilization Solution Kit (BD Pharmingen) or Transcription Factor Buffer Set (BD Pharmingen), respectively. .. All samples were run on a Beckman-Coulter CyAN ADP Analyzer (Beckman-Coulter, Brea, CA, USA), and data were analyzed by FlowJo software (TreeStar, Ashland, OR, USA).

    Article Title: Cryo-thermal therapy induces macrophage polarization for durable anti-tumor immunity
    Article Snippet: .. True-Nuclear™ Transcription Factor Buffer Set, Fixation Buffer, Intracellular Staining Permeabilization Wash Buffer, and Cell Activation Cocktail (with Brefeldin A) were all purchased from Biolegend. .. The cells were analyzed on a FACS Aria II cytometer (BD Biosciences) and the data were analyzed using FlowJo software.

    Expressing:

    Article Title: CD226 Attenuates Treg Proliferation via Akt and Erk Signaling in an EAE Model
    Article Snippet: .. To examine the intracellular expression of the cytokines IFN-γ (PE anti-mouse IFN-γ, 505807, Biolegend, San Diego, CA, USA), IL-4 (PE anti-mouse IL-4, 504104, Biolegend, San Diego, CA, USA), IL-10 (PE anti-mouse IL-10, 505008, Biolegend, San Diego, CA, USA), and IL-17A (PE anti-mouse IL-17A, 506904, Biolegend, San Diego, CA, USA), the cells were stimulated with Cell Activation Cocktail (with Brefeldin A) (423303, Biolegend, San Diego, CA, USA) for 6 h according to the manufacturer's protocols. .. To determine the amount of Foxp3+ and Ki67+ cells in the population, the cells were sequentially fixed, permeabilized (Fixation/permeabilization Diluent, 00-5223, eBioscience, San Diego, CA, USA) and stained with Foxp3 (Alexa Fluor 488 anti-mouse FOXP3, 320011, Biolegend, San Diego, CA, USA) or Ki67 (PE anti-mouse Ki67, 652403, Biolegend, San Diego, CA, USA).

    Activation Assay:

    Article Title: Transcriptional profiling and therapeutic targeting of oxidative stress in neuroinflammation.
    Article Snippet: .. Oxidative stress is a central part of innate immune-induced neurodegeneration. .. Oxidative stress is a central part of innate immune-induced neurodegeneration.

    Article Title: A salt-sensing kinase in T lymphocytes, SGK1, drives hypertension and hypertensive end-organ damage
    Article Snippet: .. Approximately 3 × 106 cells were resuspended in RPMI medium containing 5% FBS and subsequently stimulated with 4 μl of Cell Activation Cocktail containing PMA, ionomycin, and the Golgi inhibitor brefeldin A (BioLegend) at 37°C for 3 hours. .. Cells were then washed and stained first with LIVE/DEAD Fixable Violet dead cell stain (Life Technologies).

    Article Title: CD147 blockade as a potential and novel treatment of graft rejection
    Article Snippet: .. Fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD4 and phycoerythrin (PE)-conjugated mouse anti-human CD3 antibodies, Foxp3 staining buffer and a cell activation cocktail used for intracellular staining were purchased from BioLegend. .. PE-Cy7-conjugated rat anti-mouse IFN-γ, PE-conjugated rat anti-mouse IL-4, Alexa 647-conjugated rat anti-mouse IL-17, FITC-conjugated rat anti-mouse CD4, allophycocynanin-H7-conjugated rat anti-mouse CD8, PE-Cy7-conjugated rat anti-mouse CD44, BUV737-conjugated rat anti-mouse CD62 L, allophycocyanin-conjugated rat anti-mouse CD25, and PE-conjugated rat anti-mouse Foxp3 antibodies were purchased from BD Biosciences (San Diego, CA, USA).

    Article Title: CD226 Attenuates Treg Proliferation via Akt and Erk Signaling in an EAE Model
    Article Snippet: .. To examine the intracellular expression of the cytokines IFN-γ (PE anti-mouse IFN-γ, 505807, Biolegend, San Diego, CA, USA), IL-4 (PE anti-mouse IL-4, 504104, Biolegend, San Diego, CA, USA), IL-10 (PE anti-mouse IL-10, 505008, Biolegend, San Diego, CA, USA), and IL-17A (PE anti-mouse IL-17A, 506904, Biolegend, San Diego, CA, USA), the cells were stimulated with Cell Activation Cocktail (with Brefeldin A) (423303, Biolegend, San Diego, CA, USA) for 6 h according to the manufacturer's protocols. .. To determine the amount of Foxp3+ and Ki67+ cells in the population, the cells were sequentially fixed, permeabilized (Fixation/permeabilization Diluent, 00-5223, eBioscience, San Diego, CA, USA) and stained with Foxp3 (Alexa Fluor 488 anti-mouse FOXP3, 320011, Biolegend, San Diego, CA, USA) or Ki67 (PE anti-mouse Ki67, 652403, Biolegend, San Diego, CA, USA).

    Article Title: MUC1-induced immunosuppression in colon cancer can be reversed by blocking the PD1/PDL1 signaling pathway
    Article Snippet: .. For the intracellular staining of immune cells, 1×106 cells/ml were treated with Cell Activation Cocktail containing Brefeldin A (1:1,000; cat. no. 423304; BioLegend, Inc.). .. After 4 h, the cells were collected and stained with the aforementioned Brilliant Violet 510™- or FITC-conjugated mAbs against mouse or human CD45, CD4 or CD8 mAbs (BioLegend, Inc) at 4°C for 30 min.

    Article Title: Microwave ablation combined with OK-432 induces Th1-type response and specific antitumor immunity in a murine model of breast cancer
    Article Snippet: .. For intracellular cytokine staining, harvested cells were stimulated with phorbol myristate acetate (PMA), ionomycin, and brefeldin A (Cell Activation Cocktail, Biolegend, San Diego, USA) for 5 h. FITC-anti-CD4 (RM4-5), APC/Cy7-anti-CD8a (53-6.7), and matched isotype control antibodies were purchased from BD Biosciences. .. PerCP/Cy5.5-anti-CD4 (RM4-5), FITC-anti-IFN-γ (XMG1.2), APC-anti- interleukin (IL)-4 (11B11), and matched isotype control antibodies were purchased from Biolegend.

    Article Title: Decidual RANKL/RANK interaction promotes the residence and polarization of TGF-β1-producing regulatory γδ T cells
    Article Snippet: .. Intracellular cytokines were detected after stimulating with Cell Activation Cocktail (Biolegend) for 6 h. Intracellular proteins or intranuclear transcription factors were stained using Fixation/Permeabilization Solution Kit (BD Pharmingen) or Transcription Factor Buffer Set (BD Pharmingen), respectively. .. All samples were run on a Beckman-Coulter CyAN ADP Analyzer (Beckman-Coulter, Brea, CA, USA), and data were analyzed by FlowJo software (TreeStar, Ashland, OR, USA).

    Article Title: Cryo-thermal therapy induces macrophage polarization for durable anti-tumor immunity
    Article Snippet: .. True-Nuclear™ Transcription Factor Buffer Set, Fixation Buffer, Intracellular Staining Permeabilization Wash Buffer, and Cell Activation Cocktail (with Brefeldin A) were all purchased from Biolegend. .. The cells were analyzed on a FACS Aria II cytometer (BD Biosciences) and the data were analyzed using FlowJo software.

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  • 99
    BioLegend premixed pma ionomycin
    Cytokine production by novel ILCs. C10 and C2 skew towards NK-type cytokines. Lineage Negative (CD3, CD14, CD19), CD34 − CD45 + cells were activated with <t>PMA/Ionomycin</t> for 6 h. Cytokine production was assessed via intracellular labeling. (A) Representative gating scheme of CD56 Bright CD94 + CD16 − CD127 − CD49a − (C10), CD49 + (C2), and of CD56 Dim CD16 + CD127 − CD117 − from the decidua basalis showing NK type cytokine production (B–E) Quantification of NK type cytokine production by C10, C2, and cNK. Decidua basalis, n = 24; decidua parietalis, n = 9. Data represented as max/min, median, and 25 and 75th percentiles. Statistical significance was determined by ANOVA, followed by Tukey post-hoc tests. * p
    Premixed Pma Ionomycin, supplied by BioLegend, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/premixed pma ionomycin/product/BioLegend
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    premixed pma ionomycin - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    Image Search Results


    Cytokine production by novel ILCs. C10 and C2 skew towards NK-type cytokines. Lineage Negative (CD3, CD14, CD19), CD34 − CD45 + cells were activated with PMA/Ionomycin for 6 h. Cytokine production was assessed via intracellular labeling. (A) Representative gating scheme of CD56 Bright CD94 + CD16 − CD127 − CD49a − (C10), CD49 + (C2), and of CD56 Dim CD16 + CD127 − CD117 − from the decidua basalis showing NK type cytokine production (B–E) Quantification of NK type cytokine production by C10, C2, and cNK. Decidua basalis, n = 24; decidua parietalis, n = 9. Data represented as max/min, median, and 25 and 75th percentiles. Statistical significance was determined by ANOVA, followed by Tukey post-hoc tests. * p

    Journal: Frontiers in Immunology

    Article Title: Transcriptional and Functional Programming of Decidual Innate Lymphoid Cells

    doi: 10.3389/fimmu.2019.03065

    Figure Lengend Snippet: Cytokine production by novel ILCs. C10 and C2 skew towards NK-type cytokines. Lineage Negative (CD3, CD14, CD19), CD34 − CD45 + cells were activated with PMA/Ionomycin for 6 h. Cytokine production was assessed via intracellular labeling. (A) Representative gating scheme of CD56 Bright CD94 + CD16 − CD127 − CD49a − (C10), CD49 + (C2), and of CD56 Dim CD16 + CD127 − CD117 − from the decidua basalis showing NK type cytokine production (B–E) Quantification of NK type cytokine production by C10, C2, and cNK. Decidua basalis, n = 24; decidua parietalis, n = 9. Data represented as max/min, median, and 25 and 75th percentiles. Statistical significance was determined by ANOVA, followed by Tukey post-hoc tests. * p

    Article Snippet: Innate Lymphoid Cell Activation Sorted ILCs were incubated for 24 h in complete culture media (RPMI with 10% FBS, 1% Pen/Strep, 2 mM Glutamine, 25 mM HEPES) supplemented with premixed PMA/Ionomycin (Cell Activation Cocktail without Brefeldin A, BioLegend, cat#423301, ) according to manufacturer's instructions.

    Techniques: Labeling

    Polyfunctional properties of Novel ILCs. Novel ILCs C10 and C2 display unique polyfunction properties upon PMA/Ionomycin or cytokine (IL-12/IL-15/IL-1β) activation. (A) Pie charts show the proportion of functions that C10, C2, and cNK display after activation with PMA/Ionomycin (top) and cytokines (bottom). Arcs indicate the proportion that produce indicated factor IL-17A, INFγ, TNFα, VEGF. (B) Proportion of positive cells for given combinations of assessed factors after PMA/Ionomyin or cytokine activation. n = 13. Data represented as mean ± SEM. Statistical significance was determined by Student's t -test. ** p

    Journal: Frontiers in Immunology

    Article Title: Transcriptional and Functional Programming of Decidual Innate Lymphoid Cells

    doi: 10.3389/fimmu.2019.03065

    Figure Lengend Snippet: Polyfunctional properties of Novel ILCs. Novel ILCs C10 and C2 display unique polyfunction properties upon PMA/Ionomycin or cytokine (IL-12/IL-15/IL-1β) activation. (A) Pie charts show the proportion of functions that C10, C2, and cNK display after activation with PMA/Ionomycin (top) and cytokines (bottom). Arcs indicate the proportion that produce indicated factor IL-17A, INFγ, TNFα, VEGF. (B) Proportion of positive cells for given combinations of assessed factors after PMA/Ionomyin or cytokine activation. n = 13. Data represented as mean ± SEM. Statistical significance was determined by Student's t -test. ** p

    Article Snippet: Innate Lymphoid Cell Activation Sorted ILCs were incubated for 24 h in complete culture media (RPMI with 10% FBS, 1% Pen/Strep, 2 mM Glutamine, 25 mM HEPES) supplemented with premixed PMA/Ionomycin (Cell Activation Cocktail without Brefeldin A, BioLegend, cat#423301, ) according to manufacturer's instructions.

    Techniques: Activation Assay

    Unique ILCs respond to cytokine stimulation. C10, C2, and cNKs were stimulated with PMA/Ionomycin, IL-12/IL-15/IL-1β, or IL-12/IL-15/IL-18 and production of (A) INFγ, (B) Granzyme B, (C) TNFα, and (D) VEGF was assessed. Data represented as max/min, median, and 25 and 75th percentiles. Statistical significance was determined by ANOVA followed by Tukey test and are demonstrated by letters, with different letters indicating statistical differences within a subset (

    Journal: Frontiers in Immunology

    Article Title: Transcriptional and Functional Programming of Decidual Innate Lymphoid Cells

    doi: 10.3389/fimmu.2019.03065

    Figure Lengend Snippet: Unique ILCs respond to cytokine stimulation. C10, C2, and cNKs were stimulated with PMA/Ionomycin, IL-12/IL-15/IL-1β, or IL-12/IL-15/IL-18 and production of (A) INFγ, (B) Granzyme B, (C) TNFα, and (D) VEGF was assessed. Data represented as max/min, median, and 25 and 75th percentiles. Statistical significance was determined by ANOVA followed by Tukey test and are demonstrated by letters, with different letters indicating statistical differences within a subset (

    Article Snippet: Innate Lymphoid Cell Activation Sorted ILCs were incubated for 24 h in complete culture media (RPMI with 10% FBS, 1% Pen/Strep, 2 mM Glutamine, 25 mM HEPES) supplemented with premixed PMA/Ionomycin (Cell Activation Cocktail without Brefeldin A, BioLegend, cat#423301, ) according to manufacturer's instructions.

    Techniques:

    Activation of C10 and C2 ILCs with PMA/Ionomycin Reveals Preference for TNFα. Activation of C10 and C2 led to a higher proportion of TNFα + cells compared to cNKs. Decidua basalis, n = 19; decidua parietalis, n = 7. Data represented as mean ± SEM. Statistical significance was determined by ANOVA followed by Tukey test. * p

    Journal: Frontiers in Immunology

    Article Title: Transcriptional and Functional Programming of Decidual Innate Lymphoid Cells

    doi: 10.3389/fimmu.2019.03065

    Figure Lengend Snippet: Activation of C10 and C2 ILCs with PMA/Ionomycin Reveals Preference for TNFα. Activation of C10 and C2 led to a higher proportion of TNFα + cells compared to cNKs. Decidua basalis, n = 19; decidua parietalis, n = 7. Data represented as mean ± SEM. Statistical significance was determined by ANOVA followed by Tukey test. * p

    Article Snippet: Innate Lymphoid Cell Activation Sorted ILCs were incubated for 24 h in complete culture media (RPMI with 10% FBS, 1% Pen/Strep, 2 mM Glutamine, 25 mM HEPES) supplemented with premixed PMA/Ionomycin (Cell Activation Cocktail without Brefeldin A, BioLegend, cat#423301, ) according to manufacturer's instructions.

    Techniques: Activation Assay

    Production of VEGF by novel ILCs C10 and C2. Lineage Negative (CD3, CD14, CD19), CD34 − CD45 + cells were activated with PMA/Ionomycin for 6 h. (A) Left, Representative gating scheme of C10, C2, and cNK showing VEGF production. Right, Fluorescence minus one control for VEGF staining. (B) Quantification of VEGF production by C10, C2, and cNK. Decidua basalis, n = 13; decidua parietalis, n = 3. Data represented as max/min, median, and 25 and 75th percentiles. Statistical significance was determined by ANOVA, followed by Tukey post-hoc tests. ** p

    Journal: Frontiers in Immunology

    Article Title: Transcriptional and Functional Programming of Decidual Innate Lymphoid Cells

    doi: 10.3389/fimmu.2019.03065

    Figure Lengend Snippet: Production of VEGF by novel ILCs C10 and C2. Lineage Negative (CD3, CD14, CD19), CD34 − CD45 + cells were activated with PMA/Ionomycin for 6 h. (A) Left, Representative gating scheme of C10, C2, and cNK showing VEGF production. Right, Fluorescence minus one control for VEGF staining. (B) Quantification of VEGF production by C10, C2, and cNK. Decidua basalis, n = 13; decidua parietalis, n = 3. Data represented as max/min, median, and 25 and 75th percentiles. Statistical significance was determined by ANOVA, followed by Tukey post-hoc tests. ** p

    Article Snippet: Innate Lymphoid Cell Activation Sorted ILCs were incubated for 24 h in complete culture media (RPMI with 10% FBS, 1% Pen/Strep, 2 mM Glutamine, 25 mM HEPES) supplemented with premixed PMA/Ionomycin (Cell Activation Cocktail without Brefeldin A, BioLegend, cat#423301, ) according to manufacturer's instructions.

    Techniques: Fluorescence, Staining