Structured Review

ATUM cdna
Cdna, supplied by ATUM, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdna/product/ATUM
Average 92 stars, based on 9 article reviews
Price from $9.99 to $1999.99
cdna - by Bioz Stars, 2020-08
92/100 stars

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Related Articles

Amplification:

Article Title: NG2 glial cells regulate neuroimmunological responses to maintain neuronal function and survival
Article Snippet: .. Complementary DNA (20 ng) was amplified using real time PCR. .. Real time PCR was performed in the Thermal Cycler Dice Real Time System (Takara Bio), using the KAPA SYBR qPCR Master Mix (Kapa Biosystems, Boston, MA).

Synthesized:

Article Title: Sequence and structure-specific elements of HERG mRNA determine channel synthesis and trafficking efficiency
Article Snippet: .. The desired cDNA was synthesized, and sequence was fully verified by DNA 2.0. .. All restriction enzymes were from New England Biolabs, (Ipswich, MA, USA).

Article Title: Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
Article Snippet: .. TagRFP from Entacmaea quadricolor was codon optimized for expression in gram positive bacteria with Gene Designer software and the cDNA was synthesized de novo (DNA2.0, Menlo Park, CA). .. The synthetic gene was cloned downstream of the actA promoter in the vector pPL2 and stably integrated at the tRNA Arg locus of the bacterial chromosome in the as described previously .

Real-time Polymerase Chain Reaction:

Article Title: NG2 glial cells regulate neuroimmunological responses to maintain neuronal function and survival
Article Snippet: .. Complementary DNA (20 ng) was amplified using real time PCR. .. Real time PCR was performed in the Thermal Cycler Dice Real Time System (Takara Bio), using the KAPA SYBR qPCR Master Mix (Kapa Biosystems, Boston, MA).

Sequencing:

Article Title: The C‐terminal cytosolic domain of the human zinc transporter ZnT8 and its diabetes risk variant
Article Snippet: .. The sequence encoding residues 267–369 of human R325 ZnT8 (ZnT8cR; any residue numbering refers to full‐length human ZnT8 long isoform, Ensembl transcript SLC30A8‐002 ) was optimised for E. coli expression and the cDNA synthesised by DNA2.0 (Menlo Park, CA, USA). ..

Article Title: Sequence and structure-specific elements of HERG mRNA determine channel synthesis and trafficking efficiency
Article Snippet: .. The desired cDNA was synthesized, and sequence was fully verified by DNA 2.0. .. All restriction enzymes were from New England Biolabs, (Ipswich, MA, USA).

Expressing:

Article Title: The C‐terminal cytosolic domain of the human zinc transporter ZnT8 and its diabetes risk variant
Article Snippet: .. The sequence encoding residues 267–369 of human R325 ZnT8 (ZnT8cR; any residue numbering refers to full‐length human ZnT8 long isoform, Ensembl transcript SLC30A8‐002 ) was optimised for E. coli expression and the cDNA synthesised by DNA2.0 (Menlo Park, CA, USA). ..

Article Title: Combination of secretin and fluvastatin ameliorates the polyuria associated with X-linked nephrogenic diabetes insipidus in mice
Article Snippet: .. The setup of reactions consisted of 1 μl of complementary DNA (20 ng), 1 μl of TaqMan Gene Expression Assay Mix, part no.: 4351370, 10 μl TaqMan Gene Expression Master Mix, catalog no.: 4369016, and 8 μl of Rnase-free water under the following PCR conditions: step 1, 95 °C for 10 min; step 2, 95 °C for 15 s; and step 3, 60 °C for 1 min; steps 2 and 3 were repeated 40 times. .. The relative quantification of target gene expression was evaluated with data from the Step-ONE software (Foster City, CA), using the arithmetic formula 2–ΔΔCt , according to the comparative Ct method, which represents the amount of target, as normalized to the glyceraldehyde 3-phosphate dehydrogenase endogenous control (reference).

Article Title: Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
Article Snippet: .. TagRFP from Entacmaea quadricolor was codon optimized for expression in gram positive bacteria with Gene Designer software and the cDNA was synthesized de novo (DNA2.0, Menlo Park, CA). .. The synthetic gene was cloned downstream of the actA promoter in the vector pPL2 and stably integrated at the tRNA Arg locus of the bacterial chromosome in the as described previously .

Polymerase Chain Reaction:

Article Title: Combination of secretin and fluvastatin ameliorates the polyuria associated with X-linked nephrogenic diabetes insipidus in mice
Article Snippet: .. The setup of reactions consisted of 1 μl of complementary DNA (20 ng), 1 μl of TaqMan Gene Expression Assay Mix, part no.: 4351370, 10 μl TaqMan Gene Expression Master Mix, catalog no.: 4369016, and 8 μl of Rnase-free water under the following PCR conditions: step 1, 95 °C for 10 min; step 2, 95 °C for 15 s; and step 3, 60 °C for 1 min; steps 2 and 3 were repeated 40 times. .. The relative quantification of target gene expression was evaluated with data from the Step-ONE software (Foster City, CA), using the arithmetic formula 2–ΔΔCt , according to the comparative Ct method, which represents the amount of target, as normalized to the glyceraldehyde 3-phosphate dehydrogenase endogenous control (reference).

Software:

Article Title: Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
Article Snippet: .. TagRFP from Entacmaea quadricolor was codon optimized for expression in gram positive bacteria with Gene Designer software and the cDNA was synthesized de novo (DNA2.0, Menlo Park, CA). .. The synthetic gene was cloned downstream of the actA promoter in the vector pPL2 and stably integrated at the tRNA Arg locus of the bacterial chromosome in the as described previously .