cdna synthesis  (Thermo Fisher)


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    Name:
    Oligo d T 16 50 µM
    Description:
    Invitrogen Oligo d T 16 is a homo oligomeric deoxyribonucleotide poly dT that is non phosphorylated and chemically synthesized It is used for priming and reverse transcription of polyadenylated poly A mRNA Five nmoles of primer is supplied at a concentration of 50 µM
    Catalog Number:
    n8080128
    Price:
    None
    Category:
    Oligos Primers Probes Nucleotides
    Applications:
    PCR & Real-Time PCR|RT-PCR|Reverse Transcription|Two-Step RT-PCR
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    Structured Review

    Thermo Fisher cdna synthesis
    qPCR analysis of cpxP , degP , ompF , aroK , ompC , and ppiD transcript levels. <t>RNA</t> was isolated from late log (OD 600 of 0.8) cultures of MC4100 and TR10 ( cpxA24 ) and converted to <t>cDNA.</t> The cDNA was subjected to qPCR analysis, and PCR product was quantified
    Invitrogen Oligo d T 16 is a homo oligomeric deoxyribonucleotide poly dT that is non phosphorylated and chemically synthesized It is used for priming and reverse transcription of polyadenylated poly A mRNA Five nmoles of primer is supplied at a concentration of 50 µM
    https://www.bioz.com/result/cdna synthesis/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cdna synthesis - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "Characterization of the Cpx Regulon in Escherichia coli Strain MC4100 ▿"

    Article Title: Characterization of the Cpx Regulon in Escherichia coli Strain MC4100 ▿

    Journal:

    doi: 10.1128/JB.00798-08

    qPCR analysis of cpxP , degP , ompF , aroK , ompC , and ppiD transcript levels. RNA was isolated from late log (OD 600 of 0.8) cultures of MC4100 and TR10 ( cpxA24 ) and converted to cDNA. The cDNA was subjected to qPCR analysis, and PCR product was quantified
    Figure Legend Snippet: qPCR analysis of cpxP , degP , ompF , aroK , ompC , and ppiD transcript levels. RNA was isolated from late log (OD 600 of 0.8) cultures of MC4100 and TR10 ( cpxA24 ) and converted to cDNA. The cDNA was subjected to qPCR analysis, and PCR product was quantified

    Techniques Used: Real-time Polymerase Chain Reaction, Isolation, Polymerase Chain Reaction

    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Mda-9/Syntenin Is Expressed in Uveal Melanoma and Correlates with Metastatic Progression
    Article Snippet: .. RT-PCR analysis cDNA was synthesized with oligo dT primers from 2 micrograms of total RNA with Superscript II (RT Invitrogen). .. For conventional RT-PCR two microliters of cDNA were amplified with 2.5 IU of Taq Polymerase (Roche), by using the following primers: Human SDCBP upper primer: 5′TGG TGG CTC CTG TAA CTG GTA A , lower primer: 3′TGC ATG GTA ATC GTC CGT TCA A .

    Synthesized:

    Article Title: Mda-9/Syntenin Is Expressed in Uveal Melanoma and Correlates with Metastatic Progression
    Article Snippet: .. RT-PCR analysis cDNA was synthesized with oligo dT primers from 2 micrograms of total RNA with Superscript II (RT Invitrogen). .. For conventional RT-PCR two microliters of cDNA were amplified with 2.5 IU of Taq Polymerase (Roche), by using the following primers: Human SDCBP upper primer: 5′TGG TGG CTC CTG TAA CTG GTA A , lower primer: 3′TGC ATG GTA ATC GTC CGT TCA A .

    Quantitative RT-PCR:

    Article Title: Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI-60 cancer cells
    Article Snippet: Finally, the real-time RT-PCR results for five transcripts of the ABC transporter family are provided (Additional data file ). .. Supplementary Material Additional data file 1 A figure that shows scatter plots of cell-cell Pearson correlation coefficients relating the cDNA array and oligo array data sets Click here for additional data file Additional data file 2 Scatter plots of cell standard deviations across the set of 2,344 genes for the cDNA array and oligo array data sets Click here for additional data file Additional data file 3 An Excel sheet for the original Affymetrix oligo array data on 6,810 human and 319 control transcripts Click here for additional data file Additional data file 4 An Excel sheet for the ratio data on 9,706 cDNA-clone database Click here for additional data file Additional data file 5 An Excel file that gives a summary for the UniGene-matched pairs Click here for additional data file Additional data file 6 An Excel file for the consensus data set based on both cDNA- and oligo-arrays Click here for additional data file Additional data file 7 Descriptive information on the oligo-array transcripts listed in Additional data file 8 Click here for additional data file Additional data file 8 An excel file contains the Oligo database Click here for additional data file Additional data file 9 A table showing mapping coordinates for human PPs Click here for additional data file Additional data file 10 Descriptive information on the cDNA-array transcripts listed in Additional data file 10 Click here for additional data file Additional data file 11 The real-time RT-PCR results for five transcripts of the ABC transporter family Click here for additional data file ..

    Amplification:

    Article Title: Microarray analysis of defined Mycobacterium tuberculosis populations using RNA amplification strategies
    Article Snippet: The products from five ADP (+/- T7 sequences) primed RT-labelling reactions were hybridised independently against genomic DNA to the M. tuberculosis microarray (as described below), and the signal from the RNA-derived products compared to measure the effect of the additional T7 sequence on ADP primer specificity. .. RNA amplification 50 μM M. tb amplification-directed primers (ADP) were used in the place of oligo-dT in the MessageAmp II eukaryotic amplification system (Ambion); the second priming strategy included an initial polyadenylation step (with E. coli polyA polymerase at 37°C for 15 minutes) followed by oligo-dT based amplification (MessageAmp II Bacteria, Ambion). .. Amplification reactions were conducted according to manufacturers instructions, with inputs of 500 ng, 50 ng and 5 ng total mycobacterial RNA.

    Transfection:

    Article Title: MyoD induced enhancer RNA interacts with hnRNPL to activate target gene transcription during myogenic differentiation
    Article Snippet: In each case, the concentration used for transient transfections was 100 nM. .. Transient transfection of cells with siRNA oligos or DNA constructs was performed on 60 or 100 mm dishes or six-well plate with Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocol. .. Sequences of siRNA oligos are listed in Supplementary Data .

    Construct:

    Article Title: MyoD induced enhancer RNA interacts with hnRNPL to activate target gene transcription during myogenic differentiation
    Article Snippet: In each case, the concentration used for transient transfections was 100 nM. .. Transient transfection of cells with siRNA oligos or DNA constructs was performed on 60 or 100 mm dishes or six-well plate with Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocol. .. Sequences of siRNA oligos are listed in Supplementary Data .

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  • 97
    Thermo Fisher maxima cdna kit
    CD80-Fc activates transcription factors downstream of CD28 and the TcR. A, CD80-Fc induces transcription of EGR1/2/3/4. CD3 + cells were isolated by negative selection from the blood of healthy human donors, activated with PHA for 72 hours, and then either untreated or incubated for two hours with isotype control mAb, agonist CD28 mAb, TROY-Fc, or CD80-Fc. <t>RNA</t> was then isolated, converted into <t>cDNA,</t> and the DNA analyzed by qRT-PCR for EGR1/2/3/4. Values were normalized to β-actin expression. Results are shown as fold change in expression level compared to untreated T cells. Statistical analysis was performed using Student’s t test. Data are from one of three independent experiments. B, CD3 + CD28+ Jurkat cells were treated for 30 min at 37OC with isotype mAb, agonist CD28 mAb, TROY-Fc, or CD80-Fc. Cells were then fixed and permeabilized, and stained with mAbs to pMAPK or pNF-κB followed by anti-rabbit IgG-FITC. C, Jurkat cells were untreated or treated for 30 min with agonist anti-CD28 mAb and then lysed and analyzed by western blot for phosphorylated MAPK and NFκB. Data for B and C are representative of one of two and one of three independent experiments, respectively.
    Maxima Cdna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maxima cdna kit/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    maxima cdna kit - by Bioz Stars, 2021-03
    97/100 stars
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    97
    Thermo Fisher first strand cdna
    Development of AtMYB111 -expressing transgenic tobacco lines and their phenotypic characterization. (a) Schematic representation of T-DNA region of plant expression construct carrying AtMYB111 <t>cDNA</t> in pBI121 vector used for tobacco transformation. (b) Flower color alterations in different transgenic lines. The pigmentation in petals of transgenic lines expressing AtMYB111 (1, 2 and 3) was compared with WT and empty vector (EV) transformed tobacco lines. (c) Confirmation of the presence of the transgene by PCR amplification of transgene using forward and reverse primers of AtMYB111 in different transgenic lines. (d) Expression analysis of transgene through semiquantitative RT-PCR using total <t>RNA</t> from leaves of WT, EV and different transgenic lines. (e) Total anthocyanin content in petals of WT, EV and different transgenic lines. Data are expressed as means ± SE of at least 3 independent experiments, each experiment consisting of 3 technical replicates. In (c) and (d), gels have been cropped for clarity and conciseness of the presentation. *** indicate values that differ significantly from WT at P
    First Strand Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand cdna/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    first strand cdna - by Bioz Stars, 2021-03
    97/100 stars
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    Image Search Results


    CD80-Fc activates transcription factors downstream of CD28 and the TcR. A, CD80-Fc induces transcription of EGR1/2/3/4. CD3 + cells were isolated by negative selection from the blood of healthy human donors, activated with PHA for 72 hours, and then either untreated or incubated for two hours with isotype control mAb, agonist CD28 mAb, TROY-Fc, or CD80-Fc. RNA was then isolated, converted into cDNA, and the DNA analyzed by qRT-PCR for EGR1/2/3/4. Values were normalized to β-actin expression. Results are shown as fold change in expression level compared to untreated T cells. Statistical analysis was performed using Student’s t test. Data are from one of three independent experiments. B, CD3 + CD28+ Jurkat cells were treated for 30 min at 37OC with isotype mAb, agonist CD28 mAb, TROY-Fc, or CD80-Fc. Cells were then fixed and permeabilized, and stained with mAbs to pMAPK or pNF-κB followed by anti-rabbit IgG-FITC. C, Jurkat cells were untreated or treated for 30 min with agonist anti-CD28 mAb and then lysed and analyzed by western blot for phosphorylated MAPK and NFκB. Data for B and C are representative of one of two and one of three independent experiments, respectively.

    Journal: Cancer immunology research

    Article Title: Soluble CD80 protein delays tumor growth and promotes tumor infiltrating lymphocytes

    doi: 10.1158/2326-6066.CIR-17-0026

    Figure Lengend Snippet: CD80-Fc activates transcription factors downstream of CD28 and the TcR. A, CD80-Fc induces transcription of EGR1/2/3/4. CD3 + cells were isolated by negative selection from the blood of healthy human donors, activated with PHA for 72 hours, and then either untreated or incubated for two hours with isotype control mAb, agonist CD28 mAb, TROY-Fc, or CD80-Fc. RNA was then isolated, converted into cDNA, and the DNA analyzed by qRT-PCR for EGR1/2/3/4. Values were normalized to β-actin expression. Results are shown as fold change in expression level compared to untreated T cells. Statistical analysis was performed using Student’s t test. Data are from one of three independent experiments. B, CD3 + CD28+ Jurkat cells were treated for 30 min at 37OC with isotype mAb, agonist CD28 mAb, TROY-Fc, or CD80-Fc. Cells were then fixed and permeabilized, and stained with mAbs to pMAPK or pNF-κB followed by anti-rabbit IgG-FITC. C, Jurkat cells were untreated or treated for 30 min with agonist anti-CD28 mAb and then lysed and analyzed by western blot for phosphorylated MAPK and NFκB. Data for B and C are representative of one of two and one of three independent experiments, respectively.

    Article Snippet: RNA was isolated from T cells according to the manufacturer’s protocol and quantified using a BioTek Synergy 2 microplate reader. cDNA was created from each RNA sample using the Maxima cDNA kit (Thermo Scientific) per the manufacturer’s protocol.

    Techniques: Isolation, Selection, Incubation, Quantitative RT-PCR, Expressing, Staining, Western Blot

    Development of AtMYB111 -expressing transgenic tobacco lines and their phenotypic characterization. (a) Schematic representation of T-DNA region of plant expression construct carrying AtMYB111 cDNA in pBI121 vector used for tobacco transformation. (b) Flower color alterations in different transgenic lines. The pigmentation in petals of transgenic lines expressing AtMYB111 (1, 2 and 3) was compared with WT and empty vector (EV) transformed tobacco lines. (c) Confirmation of the presence of the transgene by PCR amplification of transgene using forward and reverse primers of AtMYB111 in different transgenic lines. (d) Expression analysis of transgene through semiquantitative RT-PCR using total RNA from leaves of WT, EV and different transgenic lines. (e) Total anthocyanin content in petals of WT, EV and different transgenic lines. Data are expressed as means ± SE of at least 3 independent experiments, each experiment consisting of 3 technical replicates. In (c) and (d), gels have been cropped for clarity and conciseness of the presentation. *** indicate values that differ significantly from WT at P

    Journal: Scientific Reports

    Article Title: Expression of Arabidopsis MYB transcription factor, AtMYB111, in tobacco requires light to modulate flavonol content

    doi: 10.1038/srep05018

    Figure Lengend Snippet: Development of AtMYB111 -expressing transgenic tobacco lines and their phenotypic characterization. (a) Schematic representation of T-DNA region of plant expression construct carrying AtMYB111 cDNA in pBI121 vector used for tobacco transformation. (b) Flower color alterations in different transgenic lines. The pigmentation in petals of transgenic lines expressing AtMYB111 (1, 2 and 3) was compared with WT and empty vector (EV) transformed tobacco lines. (c) Confirmation of the presence of the transgene by PCR amplification of transgene using forward and reverse primers of AtMYB111 in different transgenic lines. (d) Expression analysis of transgene through semiquantitative RT-PCR using total RNA from leaves of WT, EV and different transgenic lines. (e) Total anthocyanin content in petals of WT, EV and different transgenic lines. Data are expressed as means ± SE of at least 3 independent experiments, each experiment consisting of 3 technical replicates. In (c) and (d), gels have been cropped for clarity and conciseness of the presentation. *** indicate values that differ significantly from WT at P

    Article Snippet: Total RNA was subjected to the reverse transcription reaction to generate first-strand cDNA using oligo(dT) primers (Fermentas Life Sciences, Ontario, Canada).

    Techniques: Expressing, Transgenic Assay, Construct, Plasmid Preparation, Transformation Assay, Polymerase Chain Reaction, Amplification, Reverse Transcription Polymerase Chain Reaction

    CpG methylation status in the 5′ and 3′-LTR regions of BLV proviruses integrated in different infected cell lines. A , mRNA expression levels in the BLV-infected YR2, L267, and L267 LTaxSN cell lines. Total RNA samples were extracted and digested by DNase I. First strand cDNA was synthesized by reverse transcription, and quantitative PCR was performed with oligonucleotide primers amplifying the 5′-UTR region of BLV or the β-actin gene. Results are presented as the ratio of 5′-UTR to β-actin and are the mean values of triplicate samples. The results from a representative experiment of three independent experiments are shown. B , ex vivo expression of BLV in infected YR2, L267, and L267 LTaxSN cell lines. The BLV p24 major gag antigen levels were titrated in the culture supernatants by ELISA. Data are the mean values of triplicate samples. The results from a representative experiment of three independent ELISA experiments are shown. C , bisulfite sequencing analysis of the CpG methylation status in the 5′- and 3′-LTRs of the YR2, L267, and L267 LTaxSN cell lines. Genomic DNA from the three cell lines was extracted and treated by sodium bisulfite/hydroquinone. The two LTRs were amplified by nested PCR, amplified PCR products were subcloned in a TA cloning vector, and 10–11 clones were sequenced for each cell line. The methylation status of these clones is presented for each cell line. The positions and orientations of PCR primers ( NA , NB , NC , ND , NE , NF , NG , and NH ) used to amplify bisulfite-treated BLV DNA by nested PCR are indicated by arrows. Open circles represent unmethylated CpG dinucleotides, and filled circles represent methylated CpG dinucleotides. The −154 CpG and −129 CpG (located within the CRE1 and CRE2 sites, respectively) are hypermethylated in the L267 cell line and are indicated by asterisks .

    Journal: The Journal of Biological Chemistry

    Article Title: DNA Cytosine Methylation in the Bovine Leukemia Virus Promoter Is Associated with Latency in a Lymphoma-derived B-cell Line

    doi: 10.1074/jbc.M110.107607

    Figure Lengend Snippet: CpG methylation status in the 5′ and 3′-LTR regions of BLV proviruses integrated in different infected cell lines. A , mRNA expression levels in the BLV-infected YR2, L267, and L267 LTaxSN cell lines. Total RNA samples were extracted and digested by DNase I. First strand cDNA was synthesized by reverse transcription, and quantitative PCR was performed with oligonucleotide primers amplifying the 5′-UTR region of BLV or the β-actin gene. Results are presented as the ratio of 5′-UTR to β-actin and are the mean values of triplicate samples. The results from a representative experiment of three independent experiments are shown. B , ex vivo expression of BLV in infected YR2, L267, and L267 LTaxSN cell lines. The BLV p24 major gag antigen levels were titrated in the culture supernatants by ELISA. Data are the mean values of triplicate samples. The results from a representative experiment of three independent ELISA experiments are shown. C , bisulfite sequencing analysis of the CpG methylation status in the 5′- and 3′-LTRs of the YR2, L267, and L267 LTaxSN cell lines. Genomic DNA from the three cell lines was extracted and treated by sodium bisulfite/hydroquinone. The two LTRs were amplified by nested PCR, amplified PCR products were subcloned in a TA cloning vector, and 10–11 clones were sequenced for each cell line. The methylation status of these clones is presented for each cell line. The positions and orientations of PCR primers ( NA , NB , NC , ND , NE , NF , NG , and NH ) used to amplify bisulfite-treated BLV DNA by nested PCR are indicated by arrows. Open circles represent unmethylated CpG dinucleotides, and filled circles represent methylated CpG dinucleotides. The −154 CpG and −129 CpG (located within the CRE1 and CRE2 sites, respectively) are hypermethylated in the L267 cell line and are indicated by asterisks .

    Article Snippet: First strand cDNA was synthesized using SuperScript III reverse transcriptase (Invitrogen).

    Techniques: CpG Methylation Assay, Infection, Expressing, Synthesized, Real-time Polymerase Chain Reaction, Ex Vivo, Enzyme-linked Immunosorbent Assay, Methylation Sequencing, Amplification, Nested PCR, Polymerase Chain Reaction, TA Cloning, Plasmid Preparation, Clone Assay, Methylation

    RNA extraction and cDNA synthesis

    Journal: The Journal of Experimental Biology

    Article Title: Muscular tissues of the squid Doryteuthis pealeii express identical myosin heavy chain isoforms: an alternative mechanism for tuning contractile speed

    doi: 10.1242/jeb.064055

    Figure Lengend Snippet: RNA extraction and cDNA synthesis

    Article Snippet: First strand cDNA synthesis was performed using 125–250 ng total RNA with the Moloney murine leukemia virus reverse transcriptase (M-MuLV RT) from the Phusion RT-PCR Kit according to the manufacturer's instructions (Finnzymes, Espoo, Finland).

    Techniques: RNA Extraction