cdna synthesis  (TaKaRa)


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    Structured Review

    TaKaRa cdna synthesis
    <t>RNA</t> purification, <t>cDNA</t> synthesis, and qRT-PCR.
    Cdna Synthesis, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna synthesis/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cdna synthesis - by Bioz Stars, 2021-04
    86/100 stars

    Images

    1) Product Images from "Long Noncoding RNA MRUL Promotes ABCB1 Expression in Multidrug-Resistant Gastric Cancer Cell Sublines"

    Article Title: Long Noncoding RNA MRUL Promotes ABCB1 Expression in Multidrug-Resistant Gastric Cancer Cell Sublines

    Journal: Molecular and Cellular Biology

    doi: 10.1128/MCB.01580-13

    RNA purification, cDNA synthesis, and qRT-PCR.
    Figure Legend Snippet: RNA purification, cDNA synthesis, and qRT-PCR.

    Techniques Used: Purification, Quantitative RT-PCR

    2) Product Images from "Identification of genes involved in interactions between Biomphalaria glabrata and Schistosoma mansoni by suppression subtractive hybridization"

    Article Title: Identification of genes involved in interactions between Biomphalaria glabrata and Schistosoma mansoni by suppression subtractive hybridization

    Journal: Molecular and Biochemical Parasitology

    doi: 10.1016/j.molbiopara.2006.09.009

    Semi-quantitative RT-PCRs with specific primers for the confirmed differentially expressed fragments on haemocytes from the repeated experiment, exposed resistant and susceptible snails. (a) PCRs using cDNA and (b) PCRs using amplified cDNA (for those fragments not detected directly from cDNA) 0—no DNA control. For each candidate integrated optical densities were calculated from the bands and compared using a matched pairs t -test. * Significantly different expression ( p
    Figure Legend Snippet: Semi-quantitative RT-PCRs with specific primers for the confirmed differentially expressed fragments on haemocytes from the repeated experiment, exposed resistant and susceptible snails. (a) PCRs using cDNA and (b) PCRs using amplified cDNA (for those fragments not detected directly from cDNA) 0—no DNA control. For each candidate integrated optical densities were calculated from the bands and compared using a matched pairs t -test. * Significantly different expression ( p

    Techniques Used: Amplification, Expressing

    Related Articles

    other:

    Article Title: Genome-wide analysis reveals four key transcription factors associated with cadmium stress in creeping bentgrass (Agrostis stolonifera L.)
    Article Snippet: The First Strand cDNA Synthesis Kit as well as the PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara, Beijing, China) were used.

    Article Title: Carbon sources and XlnR-dependent transcriptional landscape of CAZymes in the industrial fungus Talaromyces versatilis: when exception seems to be the rule
    Article Snippet: One microgram of total RNA from mycelia samples were used for the cDNA synthesis reaction using the PrimeScript First Strand cDNA Synthesis Kit (Takara), according to the Manufacturers’ protocol.

    Article Title: Fibroblast growth factor 10 upregulates the expression of mucins in rat conjunctival epithelial cells
    Article Snippet: One microgram total RNA was used for cDNA (cDNA) synthesis using the Reverse Transcription System (Takara Bio, Otsu, Japan) according to the manufacturer’s instructions.

    Article Title: A Phytophthora effector recruits a host cytoplasmic transacetylase into nuclear speckles to enhance plant susceptibility
    Article Snippet: To synthesize first strand cDNA, we used PrimeScript1 st Strand cDNA Synthesis Kit (Takara, D6110A) with 2 μg of RNA.

    Article Title: Identification of key genes in human airway epithelial cells in response to respiratory pathogens using microarray analysis
    Article Snippet: Total RNA was used for synthesis of cDNA with the First Strand cDNA Synthesis Kit (TaKaRa).

    Article Title: Targeted release of stromal cell-derived factor-1α by reactive oxygen species-sensitive nanoparticles results in bone marrow stromal cell chemotaxis and homing, and repair of vascular injury caused by electrical burns
    Article Snippet: First Strand cDNA Synthesis Kit was purchased from Takara Bio (Dalian, China).

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    TaKaRa smart pcr cdna synthesis system
    TrkB.T1 tissue expression as fold regulation over heart expression determined by real-time quantitative <t>PCR.</t> Bar graphs represent mean ± SEM of two experiments done in triplicate. <t>cDNA</t> is normalized to the expression level of 12 different housekeeping genes.
    Smart Pcr Cdna Synthesis System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smart pcr cdna synthesis system/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    smart pcr cdna synthesis system - by Bioz Stars, 2021-04
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    TaKaRa cdna synthesis
    General biological properties of ovary-derived circRNAs. ( A ) Primer patterns of circRNAs and linear RNAs. The blocks represent exons. Red arrows represent divergent primers, blue arrows represent convergent primers, and the black vertical line represents the circRNA back-spliced junction. ( B ) CircRNAs were successfully amplified by divergent primers from <t>cDNA</t> but could not be amplified from gDNA. Linear RNAs were successfully amplified by convergent primers from both cDNA and gDNA. <t>PCR</t> products were examined by 2% agarose electrophoresis. The circCCSER2 and circATXN3 were used as experimental groups, and GAPDH was the control group. ( C ) Total RNA derived cDNA successfully amplified circRNAs, but this did not work for poly-A + RNA derived cDNAs. Four circRNAs were examined, and their linear forms were used as controls. ( D ) CircRNAs were resistant to RNase R digestion, whereas their linear forms were sensitive to RNase R digestion. Ten circRNAs were examined, and six of the corresponding linear RNAs were used as controls. ( E ) Ovary-derived circRNAs can be stably expressed in human granulosa cells (hGCs) from follicular fluid in 24 h, whereas their linear forms are degraded rapidly. ( F ) A schematic diagram of the alternative splicing. PCR products for three pairs of primers were examined by 2% agarose electrophoresis ( G ) and Sanger sequencing ( H ). CircCSE1L was explored as an example. *, P
    Cdna Synthesis, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna synthesis/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cdna synthesis - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    TrkB.T1 tissue expression as fold regulation over heart expression determined by real-time quantitative PCR. Bar graphs represent mean ± SEM of two experiments done in triplicate. cDNA is normalized to the expression level of 12 different housekeeping genes.

    Journal: Gene Expression

    Article Title: Translational Downregulation of the Noncatalytic Growth Factor Receptor TrkB.T1 by Ischemic Preconditioning of Primary Neurons

    doi:

    Figure Lengend Snippet: TrkB.T1 tissue expression as fold regulation over heart expression determined by real-time quantitative PCR. Bar graphs represent mean ± SEM of two experiments done in triplicate. cDNA is normalized to the expression level of 12 different housekeeping genes.

    Article Snippet: Virtual Northern blots were performed as described in the SMART PCR cDNA synthesis system (Clontech).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    General biological properties of ovary-derived circRNAs. ( A ) Primer patterns of circRNAs and linear RNAs. The blocks represent exons. Red arrows represent divergent primers, blue arrows represent convergent primers, and the black vertical line represents the circRNA back-spliced junction. ( B ) CircRNAs were successfully amplified by divergent primers from cDNA but could not be amplified from gDNA. Linear RNAs were successfully amplified by convergent primers from both cDNA and gDNA. PCR products were examined by 2% agarose electrophoresis. The circCCSER2 and circATXN3 were used as experimental groups, and GAPDH was the control group. ( C ) Total RNA derived cDNA successfully amplified circRNAs, but this did not work for poly-A + RNA derived cDNAs. Four circRNAs were examined, and their linear forms were used as controls. ( D ) CircRNAs were resistant to RNase R digestion, whereas their linear forms were sensitive to RNase R digestion. Ten circRNAs were examined, and six of the corresponding linear RNAs were used as controls. ( E ) Ovary-derived circRNAs can be stably expressed in human granulosa cells (hGCs) from follicular fluid in 24 h, whereas their linear forms are degraded rapidly. ( F ) A schematic diagram of the alternative splicing. PCR products for three pairs of primers were examined by 2% agarose electrophoresis ( G ) and Sanger sequencing ( H ). CircCSE1L was explored as an example. *, P

    Journal: Aging (Albany NY)

    Article Title: Identification and characterization of human ovary-derived circular RNAs and their potential roles in ovarian aging

    doi: 10.18632/aging.101565

    Figure Lengend Snippet: General biological properties of ovary-derived circRNAs. ( A ) Primer patterns of circRNAs and linear RNAs. The blocks represent exons. Red arrows represent divergent primers, blue arrows represent convergent primers, and the black vertical line represents the circRNA back-spliced junction. ( B ) CircRNAs were successfully amplified by divergent primers from cDNA but could not be amplified from gDNA. Linear RNAs were successfully amplified by convergent primers from both cDNA and gDNA. PCR products were examined by 2% agarose electrophoresis. The circCCSER2 and circATXN3 were used as experimental groups, and GAPDH was the control group. ( C ) Total RNA derived cDNA successfully amplified circRNAs, but this did not work for poly-A + RNA derived cDNAs. Four circRNAs were examined, and their linear forms were used as controls. ( D ) CircRNAs were resistant to RNase R digestion, whereas their linear forms were sensitive to RNase R digestion. Ten circRNAs were examined, and six of the corresponding linear RNAs were used as controls. ( E ) Ovary-derived circRNAs can be stably expressed in human granulosa cells (hGCs) from follicular fluid in 24 h, whereas their linear forms are degraded rapidly. ( F ) A schematic diagram of the alternative splicing. PCR products for three pairs of primers were examined by 2% agarose electrophoresis ( G ) and Sanger sequencing ( H ). CircCSE1L was explored as an example. *, P

    Article Snippet: cDNA synthesis, PCR, electrophoresis and quantitative real-time PCR (qRT-PCR) Total RNA was reverse transcribed using random primers with the PrimeScript RT reagent kit (RR047A, Takara, Dalian, China) according to the manufacturer’s protocol.

    Techniques: Derivative Assay, Amplification, Polymerase Chain Reaction, Electrophoresis, Stable Transfection, Sequencing

    RNA Extraction, cDNA Synthesis, and RT-PCR

    Journal: The Journal of Biological Chemistry

    Article Title: A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori *

    doi: 10.1074/jbc.M115.699819

    Figure Lengend Snippet: RNA Extraction, cDNA Synthesis, and RT-PCR

    Article Snippet: The Illustra RNAspin MINI RNA Isolation kit (GE Healthcare) was used for RNA extraction from hsp70 -GAL4/+ and hsp70 -GAL4/UAS- Antp individuals and was used for cDNA synthesis using a PrimeScript RT-PCR kit (Takara, Otsu, Japan) ( ).

    Techniques: RNA Extraction, Reverse Transcription Polymerase Chain Reaction

    Comparisons of alpha indices for mudflat sediments at different depths derived from amplicon and MT-16S datasets. (A) Observed OTU numbers; (B) Chao 1; (C) Shannon indices; (D) PD indices. For each dataset, 5000 sequences were randomly selected for each diversity calculation. S1, S2, and S3 represent the sampling locations; DNA and cDNA indicate 16S rDNA and 16S cDNA amplicon datasets, and Meta indicates MT-16S datasets.

    Journal: Frontiers in Microbiology

    Article Title: Microbial Communities and Diversities in Mudflat Sediments Analyzed Using a Modified Metatranscriptomic Method

    doi: 10.3389/fmicb.2018.00093

    Figure Lengend Snippet: Comparisons of alpha indices for mudflat sediments at different depths derived from amplicon and MT-16S datasets. (A) Observed OTU numbers; (B) Chao 1; (C) Shannon indices; (D) PD indices. For each dataset, 5000 sequences were randomly selected for each diversity calculation. S1, S2, and S3 represent the sampling locations; DNA and cDNA indicate 16S rDNA and 16S cDNA amplicon datasets, and Meta indicates MT-16S datasets.

    Article Snippet: cDNA Synthesis For the cDNA amplicon analyses, the total RNA from the S2 location samples was first digested with DNase I (TaKaRa, Dalian, China) for 1 h and then purified using a MinElute Cleanup Kit (Qiagen, Hilden, Germany).

    Techniques: Derivative Assay, Amplification, Sampling