cdh 11 antibody  (Thermo Fisher)


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    Name:
    CDH11 Cadherin OB Polyclonal Antibody
    Description:
    CDH11 Cadherin OB Polyclonal Antibody for Western Blot IF IP
    Catalog Number:
    A16652
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
    Applications:
    Cell Analysis|Cellular Imaging|Immunofluorescence (IF)|Immunofluorescence Staining & Detection|Immunoprecipitation|Protein Assays and Analysis|Protein Biology|Protein Purification & Isolation|Western Blotting
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    Structured Review

    Thermo Fisher cdh 11 antibody
    Fresh and cryopreserved UCT expresses <t>CDH-11</t> mRNA. (A) Fresh and (B) frozen UCT from 4 different donors were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager.
    CDH11 Cadherin OB Polyclonal Antibody for Western Blot IF IP
    https://www.bioz.com/result/cdh 11 antibody/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cdh 11 antibody - by Bioz Stars, 2021-06
    93/100 stars

    Images

    1) Product Images from "CD73, CD90, CD105 and Cadherin-11 RT-PCR Screening for Mesenchymal Stem Cells from Cryopreserved Human Cord Tissue"

    Article Title: CD73, CD90, CD105 and Cadherin-11 RT-PCR Screening for Mesenchymal Stem Cells from Cryopreserved Human Cord Tissue

    Journal: International Journal of Stem Cells

    doi: 10.15283/ijsc17015

    Fresh and cryopreserved UCT expresses CDH-11 mRNA. (A) Fresh and (B) frozen UCT from 4 different donors were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager.
    Figure Legend Snippet: Fresh and cryopreserved UCT expresses CDH-11 mRNA. (A) Fresh and (B) frozen UCT from 4 different donors were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, Agarose Gel Electrophoresis

    MSC expansion from cryopreserved UCT expresses CDH-11. MSCs expanded from (A) fresh and (B) cryopreserved UCT sections were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager. (C) MSCs were processed for protein, denatured in loading buffer containing SDS, resolved on a 4~20% pre-cast gel and transferred onto nitrocellulose membranes. Membranes were probed with CDH-11 or GAPDH antibodies, visualized by ECL and captured on an imager.
    Figure Legend Snippet: MSC expansion from cryopreserved UCT expresses CDH-11. MSCs expanded from (A) fresh and (B) cryopreserved UCT sections were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager. (C) MSCs were processed for protein, denatured in loading buffer containing SDS, resolved on a 4~20% pre-cast gel and transferred onto nitrocellulose membranes. Membranes were probed with CDH-11 or GAPDH antibodies, visualized by ECL and captured on an imager.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, Agarose Gel Electrophoresis

    Related Articles

    Blocking Assay:

    Article Title: Regulation of cadherin expression in the chicken neural crest by the Wnt/?-catenin signaling pathway
    Article Snippet: The embryo lysates were mixed with the 2X Laemmli sample buffer (Bio-Rad) and 5% BME (β-mercaptoethanol), boiled at 92°C for 3 min and loaded on to 10% gels of 1 mm thickness for which electrophoresis was carried out for 90 min at 100 V. Proteins were transferred onto PVDF (Bio-Rad) membranes at 300 mA for 90 min under cooling conditions. .. The membranes were blocked using Aqua block (EastCoast Bio) for 1 h at RT, incubated with the cadherin-11 primary antibody (mouse anti-human cadherin-11, 2 µg/mL, Invitrogen), washed with 1X TBST and incubated with the IRDye 680 nm-labeled secondary antibody, (1:5,000, LI-COR) for detection with the Odyssey infrared imaging system (LI-COR). .. Ladder: Precision Plus Protein Standards (Bio-Rad).

    Incubation:

    Article Title: Regulation of cadherin expression in the chicken neural crest by the Wnt/?-catenin signaling pathway
    Article Snippet: The embryo lysates were mixed with the 2X Laemmli sample buffer (Bio-Rad) and 5% BME (β-mercaptoethanol), boiled at 92°C for 3 min and loaded on to 10% gels of 1 mm thickness for which electrophoresis was carried out for 90 min at 100 V. Proteins were transferred onto PVDF (Bio-Rad) membranes at 300 mA for 90 min under cooling conditions. .. The membranes were blocked using Aqua block (EastCoast Bio) for 1 h at RT, incubated with the cadherin-11 primary antibody (mouse anti-human cadherin-11, 2 µg/mL, Invitrogen), washed with 1X TBST and incubated with the IRDye 680 nm-labeled secondary antibody, (1:5,000, LI-COR) for detection with the Odyssey infrared imaging system (LI-COR). .. Ladder: Precision Plus Protein Standards (Bio-Rad).

    Article Title: Trio Controls the Mature Organization of Neuronal Clusters in the Hindbrain
    Article Snippet: After three washing steps with the lysis buffer, immunoprecipitated proteins were eluted by boiling with the Laemli's loading buffer, resolved on a 6 or 10% polyacrylamide gel, and blotted on to nitrocellulose membrane (GE Healthcare). .. After saturation in TBS/Tween 20 with 5% milk, membranes were incubated with the following antibodies: anti-Notch (1:500 dilution; Calbiochem, La Jolla, CA); anti-Abl (Abelson murine leukemia viral oncogene homolog; 1:100 dilution; Calbiochem); anti-DCC (deleted in colorectal cancer) (1:500 dilution; BD Biosciences PharMingen, San Diego, CA); anti-Cadherin-11 (1:100 dilution; Zymed Laboratories, South San Francisco, CA), anti-N-Cadherin (1:1000 dilution; Transduction Laboratories, Lexington, KY), and anti-Nr-CAM (1:3000 dilution; a kind gift from Dr. M. Grumet, Rutgers, Piscataway, NJ). .. Primary antibodies were revealed by incubation with HRP-conjugated anti-mouse or anti-rabbit IgG (1:25,000; Jackson ImmunoResearch).

    Article Title: Extracellular Vesicle Transfer from Endothelial Cells Drives VE-Cadherin Expression in Breast Cancer Cells, Thereby Causing Heterotypic Cell Contacts
    Article Snippet: Lysate proteins were separated by SDS-PAGE in a 10% polyacrylamide gel and transferred by wet blotting onto nitrocellulose membranes (Whatman, Dassel, Germany). .. Membranes were incubated with the following primary antibodies: goat anti-VE-cadherin (C-19) (1:100; Santa Cruz, Dallas, TX, USA), mouse anti-VE-cadherin (BV9) (1:100; Santa Cruz), mouse anti-Cadherin 11 (1:50; Invitrogen), mouse anti-E-cadherin (1:100; BD Biosciences), or mouse anti-vimentin (1:50; BD Biosciences), followed by incubation with HRP-conjugated secondary IgG (1:1000; Dako, Hamburg, Germany). .. For detection of immunoblots with fluorescently labelled antibodies, we incubated membranes with IRDye 600 CW and 800 CW infrared secondary anti-mouse- and anti-rabbit-IgG antibodies (1:10,000, Li-COR Biotechnology, Bad Homburg, Germany).

    Imaging:

    Article Title: Regulation of cadherin expression in the chicken neural crest by the Wnt/?-catenin signaling pathway
    Article Snippet: The embryo lysates were mixed with the 2X Laemmli sample buffer (Bio-Rad) and 5% BME (β-mercaptoethanol), boiled at 92°C for 3 min and loaded on to 10% gels of 1 mm thickness for which electrophoresis was carried out for 90 min at 100 V. Proteins were transferred onto PVDF (Bio-Rad) membranes at 300 mA for 90 min under cooling conditions. .. The membranes were blocked using Aqua block (EastCoast Bio) for 1 h at RT, incubated with the cadherin-11 primary antibody (mouse anti-human cadherin-11, 2 µg/mL, Invitrogen), washed with 1X TBST and incubated with the IRDye 680 nm-labeled secondary antibody, (1:5,000, LI-COR) for detection with the Odyssey infrared imaging system (LI-COR). .. Ladder: Precision Plus Protein Standards (Bio-Rad).

    Transduction:

    Article Title: Trio Controls the Mature Organization of Neuronal Clusters in the Hindbrain
    Article Snippet: After three washing steps with the lysis buffer, immunoprecipitated proteins were eluted by boiling with the Laemli's loading buffer, resolved on a 6 or 10% polyacrylamide gel, and blotted on to nitrocellulose membrane (GE Healthcare). .. After saturation in TBS/Tween 20 with 5% milk, membranes were incubated with the following antibodies: anti-Notch (1:500 dilution; Calbiochem, La Jolla, CA); anti-Abl (Abelson murine leukemia viral oncogene homolog; 1:100 dilution; Calbiochem); anti-DCC (deleted in colorectal cancer) (1:500 dilution; BD Biosciences PharMingen, San Diego, CA); anti-Cadherin-11 (1:100 dilution; Zymed Laboratories, South San Francisco, CA), anti-N-Cadherin (1:1000 dilution; Transduction Laboratories, Lexington, KY), and anti-Nr-CAM (1:3000 dilution; a kind gift from Dr. M. Grumet, Rutgers, Piscataway, NJ). .. Primary antibodies were revealed by incubation with HRP-conjugated anti-mouse or anti-rabbit IgG (1:25,000; Jackson ImmunoResearch).

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  • About
  • News
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  • Team
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  • Bioz Stars
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  • 93
    Thermo Fisher cdh 11 antibody
    Fresh and cryopreserved UCT expresses <t>CDH-11</t> mRNA. (A) Fresh and (B) frozen UCT from 4 different donors were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager.
    Cdh 11 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdh 11 antibody/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cdh 11 antibody - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    93
    Thermo Fisher antibody diluent anti cadherin 11 antibody
    Fresh and cryopreserved UCT expresses <t>CDH-11</t> mRNA. (A) Fresh and (B) frozen UCT from 4 different donors were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager.
    Antibody Diluent Anti Cadherin 11 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody diluent anti cadherin 11 antibody/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody diluent anti cadherin 11 antibody - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    Fresh and cryopreserved UCT expresses CDH-11 mRNA. (A) Fresh and (B) frozen UCT from 4 different donors were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager.

    Journal: International Journal of Stem Cells

    Article Title: CD73, CD90, CD105 and Cadherin-11 RT-PCR Screening for Mesenchymal Stem Cells from Cryopreserved Human Cord Tissue

    doi: 10.15283/ijsc17015

    Figure Lengend Snippet: Fresh and cryopreserved UCT expresses CDH-11 mRNA. (A) Fresh and (B) frozen UCT from 4 different donors were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager.

    Article Snippet: CDH-11 antibody (Life Technologies, Carlsbad, CA), horseradish peroxidase-conjugated anti-rabbit IgG or anti-mouse IgG and ECL reagents were acquired from Thermo Fisher Scientific (Waltham, MA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Agarose Gel Electrophoresis

    MSC expansion from cryopreserved UCT expresses CDH-11. MSCs expanded from (A) fresh and (B) cryopreserved UCT sections were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager. (C) MSCs were processed for protein, denatured in loading buffer containing SDS, resolved on a 4~20% pre-cast gel and transferred onto nitrocellulose membranes. Membranes were probed with CDH-11 or GAPDH antibodies, visualized by ECL and captured on an imager.

    Journal: International Journal of Stem Cells

    Article Title: CD73, CD90, CD105 and Cadherin-11 RT-PCR Screening for Mesenchymal Stem Cells from Cryopreserved Human Cord Tissue

    doi: 10.15283/ijsc17015

    Figure Lengend Snippet: MSC expansion from cryopreserved UCT expresses CDH-11. MSCs expanded from (A) fresh and (B) cryopreserved UCT sections were processed for RNA, converted to cDNA and screened for CDH-11 by RT-PCR. GAPDH was used as an internal control and data are expressed as mean fold expression against GAPDH±SD from 3 experiments. Representative reactions were resolved on a 1.5% agarose gel and captured on an imager. (C) MSCs were processed for protein, denatured in loading buffer containing SDS, resolved on a 4~20% pre-cast gel and transferred onto nitrocellulose membranes. Membranes were probed with CDH-11 or GAPDH antibodies, visualized by ECL and captured on an imager.

    Article Snippet: CDH-11 antibody (Life Technologies, Carlsbad, CA), horseradish peroxidase-conjugated anti-rabbit IgG or anti-mouse IgG and ECL reagents were acquired from Thermo Fisher Scientific (Waltham, MA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Agarose Gel Electrophoresis