cd5 (Santa Cruz Biotechnology)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Santa Cruz Biotechnology cd5

Assessment of DEGs in peripheral Bregs. CD19 + <t>CD5</t> + Bregs were isolated from blood samples of 30 food allergy (FA) patients and 30 healthy control (HC) subjects and analyzed by RNAseq and RT-qPCR. (a) Heatmap shows the most active 10 DEGs in Bregs. (b) Violin plots show RT-qPCR results of the 10 DEGs in Bregs. The data of violin plots are presented as median (IQR). ∗∗∗ P < 0.001 (Mann-Whitney test), compared with the HC group. Each bubble in violin plots shows data obtained from one sample.

Cd5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd5/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

cd5 - by Bioz Stars, 2023-03

86/100 stars

Images

1) Product Images from "Modulating Oxidative Stress in B Cells Promotes Immunotherapy in Food Allergy"

Article Title: Modulating Oxidative Stress in B Cells Promotes Immunotherapy in Food Allergy

Journal: Oxidative Medicine and Cellular Longevity

doi: 10.1155/2022/3605977

Assessment of DEGs in peripheral Bregs. CD19 + CD5 + Bregs were isolated from blood samples of 30 food allergy (FA) patients and 30 healthy control (HC) subjects and analyzed by RNAseq and RT-qPCR. (a) Heatmap shows the most active 10 DEGs in Bregs. (b) Violin plots show RT-qPCR results of the 10 DEGs in Bregs. The data of violin plots are presented as median (IQR). ∗∗∗ P < 0.001 (Mann-Whitney test), compared with the HC group. Each bubble in violin plots shows data obtained from one sample.

Figure Legend Snippet: Assessment of DEGs in peripheral Bregs. CD19 + CD5 + Bregs were isolated from blood samples of 30 food allergy (FA) patients and 30 healthy control (HC) subjects and analyzed by RNAseq and RT-qPCR. (a) Heatmap shows the most active 10 DEGs in Bregs. (b) Violin plots show RT-qPCR results of the 10 DEGs in Bregs. The data of violin plots are presented as median (IQR). ∗∗∗ P < 0.001 (Mann-Whitney test), compared with the HC group. Each bubble in violin plots shows data obtained from one sample.

Techniques Used: Isolation, Quantitative RT-PCR, MANN-WHITNEY

FGN counteracts the specific antigen-induced Breg apoptosis. LPMCs were prepared from naïve BALB/c mice or mice treated by gavage-feeding with OVA daily for one week and exposed to OVA (or BSA) in the culture overnight. (a) LPMCs were analyzed by FACS. Gated plots show GL7 + B cells (activated B cells). (b) Gated plots show CD5 + IL-10 + cells in the gated cells in panel Ad. (c) Boxplots show activated (by OVA) B cell frequency. (d–g) CD5 + B cells were isolated from LPMCs prepared naïve mice and OVA-primed mice and cultured in the presence of OVA (or BSA) overnight. Cells were stained with propidium iodide and Annexin V reagent and analyzed by FACS. Gated plots show (d) apoptotic cells. Boxplots show apoptotic (e) Breg frequency or (f) FasL mRNA in Breg. Immunoblots show (g) FasL protein levels in Bregs. The data of boxplots are presented as median (IQR). ∗∗∗ P < 0.001 (ANOVA+Dunnett's test), compared with group a. Each bubble in boxplots presents data obtained from one sample.

Figure Legend Snippet: FGN counteracts the specific antigen-induced Breg apoptosis. LPMCs were prepared from naïve BALB/c mice or mice treated by gavage-feeding with OVA daily for one week and exposed to OVA (or BSA) in the culture overnight. (a) LPMCs were analyzed by FACS. Gated plots show GL7 + B cells (activated B cells). (b) Gated plots show CD5 + IL-10 + cells in the gated cells in panel Ad. (c) Boxplots show activated (by OVA) B cell frequency. (d–g) CD5 + B cells were isolated from LPMCs prepared naïve mice and OVA-primed mice and cultured in the presence of OVA (or BSA) overnight. Cells were stained with propidium iodide and Annexin V reagent and analyzed by FACS. Gated plots show (d) apoptotic cells. Boxplots show apoptotic (e) Breg frequency or (f) FasL mRNA in Breg. Immunoblots show (g) FasL protein levels in Bregs. The data of boxplots are presented as median (IQR). ∗∗∗ P < 0.001 (ANOVA+Dunnett's test), compared with group a. Each bubble in boxplots presents data obtained from one sample.

Techniques Used: Isolation, Cell Culture, Staining, Western Blot

cd5 (Santa Cruz Biotechnology)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Santa Cruz Biotechnology cd5
Clinical and pathological features of 18 SPTCL cases

cd5 - by Bioz Stars, 2023-03

86/100 stars

Images

1) Product Images from "A retrospective study of 18 children with subcutaneous panniculitis-like T-cell lymphoma: multidrug combination chemotherapy or immunomodulatory therapy?"

Article Title: A retrospective study of 18 children with subcutaneous panniculitis-like T-cell lymphoma: multidrug combination chemotherapy or immunomodulatory therapy?

Journal: Orphanet Journal of Rare Diseases

doi: 10.1186/s13023-022-02575-4

Figure Legend Snippet: Clinical and pathological features of 18 SPTCL cases

Techniques Used:

cd5 (Santa Cruz Biotechnology)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Santa Cruz Biotechnology cd5
Cd5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd5/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

cd5 - by Bioz Stars, 2023-03

86/100 stars

Images

cd5 (Santa Cruz Biotechnology)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Santa Cruz Biotechnology cd5

Lower serum PA levels are associated with lower B10 cell frequency in FA patients. Peripheral blood mononuclear cells (PBMCs) were obtained from 40 FA patients and 40 HC (healthy control) subjects, and analysed by FACS. (A) The SSC/FSC plots. (B) Gated plots show CD19 + B cell frequency. (C) Gated plots show <t>CD5</t> + CD1d + B cell frequency. (D) Violin plots show median (IQR) of CD5 + CD1d + B cells from 40 HC subjects and 40 FA subjects. (E and F) FACS plots show IL‐10 + B cells in CD19 + CD5 + CD1d + B cells (as shown in panel C). Violin plots show summarized B10 cell counts. (G) CD19 + CD5 + CD1d + B cells were isolated from PBMCs, and analysed by RT‐qPCR. Violin plots show IL‐10 mRNA levels in B10 cells. (H) B10 cells were isolated from 10 HC subjects and 10 FA subjects, and cultured in the presence of CpG (1 µg/ml) or saline overnight. Violin plots show median (IQR) of IL‐10 levels in culture supernatant from 10 tests. (I–J) Positive correlation between serum PA levels and peripheral B10 cell frequency in the HC group and the FA group. Each bubble in violin plots presents data obtained from one sample. *** p < 0.001, compared with the HC group (D), or the CD5¯ CD1d¯ B cell group (G), or the saline group (H). ### p < 0.001, compared with the HC/CpG group

cd5 - by Bioz Stars, 2023-03

86/100 stars

Images

1) Product Images from "Propionic acid regulates immune tolerant properties in B Cells"

Article Title: Propionic acid regulates immune tolerant properties in B Cells

Journal: Journal of Cellular and Molecular Medicine

doi: 10.1111/jcmm.17287

Figure Legend Snippet: Lower serum PA levels are associated with lower B10 cell frequency in FA patients. Peripheral blood mononuclear cells (PBMCs) were obtained from 40 FA patients and 40 HC (healthy control) subjects, and analysed by FACS. (A) The SSC/FSC plots. (B) Gated plots show CD19 + B cell frequency. (C) Gated plots show CD5 + CD1d + B cell frequency. (D) Violin plots show median (IQR) of CD5 + CD1d + B cells from 40 HC subjects and 40 FA subjects. (E and F) FACS plots show IL‐10 + B cells in CD19 + CD5 + CD1d + B cells (as shown in panel C). Violin plots show summarized B10 cell counts. (G) CD19 + CD5 + CD1d + B cells were isolated from PBMCs, and analysed by RT‐qPCR. Violin plots show IL‐10 mRNA levels in B10 cells. (H) B10 cells were isolated from 10 HC subjects and 10 FA subjects, and cultured in the presence of CpG (1 µg/ml) or saline overnight. Violin plots show median (IQR) of IL‐10 levels in culture supernatant from 10 tests. (I–J) Positive correlation between serum PA levels and peripheral B10 cell frequency in the HC group and the FA group. Each bubble in violin plots presents data obtained from one sample. *** p < 0.001, compared with the HC group (D), or the CD5¯ CD1d¯ B cell group (G), or the saline group (H). ### p < 0.001, compared with the HC/CpG group

Techniques Used: Isolation, Quantitative RT-PCR, Cell Culture

anti cd5 (Santa Cruz Biotechnology)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Santa Cruz Biotechnology anti cd5
Anti Cd5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd5/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

anti cd5 - by Bioz Stars, 2023-03

86/100 stars

Images

cd5 (Santa Cruz Biotechnology)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

cd5 - by Bioz Stars, 2023-03

86/100 stars

Images

anti cd5 (Santa Cruz Biotechnology)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Santa Cruz Biotechnology anti cd5
NFS-25 cells cultured with DS-AF568 (red) and stained with <t>anti-CD5</t> (green) and DAPI. DS and CD5 only partially co-localize. Each panel shows four quadrants: green (upper left), red (upper right), blue (lower left), and merged (lower right) channels.

NFS-25 cells cultured with DS-AF568 (red) and stained with <t>anti-CD5</t> (green) and DAPI. DS and CD5 only partially co-localize. Each panel shows four quadrants: green (upper left), red (upper right), blue (lower left), and merged (lower right) channels.

Anti Cd5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd5/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

anti cd5 - by Bioz Stars, 2023-03

86/100 stars

Images

1) Product Images from "Dermatan Sulfate Is a Potential Master Regulator of IgH via Interactions with Pre-BCR, GTF2I, and BiP ER Complex in Pre-B Lymphoblasts"

Article Title: Dermatan Sulfate Is a Potential Master Regulator of IgH via Interactions with Pre-BCR, GTF2I, and BiP ER Complex in Pre-B Lymphoblasts

Journal: bioRxiv

doi: 10.1101/2021.01.18.427153

NFS-25 cells cultured with DS-AF568 (red) and stained with anti-CD5 (green) and DAPI. DS and CD5 only partially co-localize. Each panel shows four quadrants: green (upper left), red (upper right), blue (lower left), and merged (lower right) channels.

Figure Legend Snippet: NFS-25 cells cultured with DS-AF568 (red) and stained with anti-CD5 (green) and DAPI. DS and CD5 only partially co-localize. Each panel shows four quadrants: green (upper left), red (upper right), blue (lower left), and merged (lower right) channels.

Techniques Used: Cell Culture, Staining

anti cd5 (Santa Cruz Biotechnology)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Santa Cruz Biotechnology anti cd5

Components of NF-κB signaling are selectively altered in CD5hi compared to CD5lo peripheral T cells. The surface levels of <t>CD5</t> and intracellular levels of Zap70 (A), PLCγ (B), LAT (C), IκBα (D), or NF-κB (E), are shown for either CD4 (Left) or CD8 (Right) T cells. The gMFI of CD5 (F), IκBα (G), or NFκB (H) expressed by CD4 or CD8 T cells from lymph node or spleen cells, gated on live, TCR-β+ cells (n = 11 from 3 independent experiments). Statistical significance was calculated using a paired t test. ***P < 0.001; anything not marked is not statistically significant. (I) The surface levels of CD5 and CD44 are shown for CD4 (Left) or CD8 (Right) T cells. (J) The distribution of IκBα expression with other cell surface markers (CD127, CD44, CD62L, TCR-β) on polyclonal peripheral T cells from lymph node or spleen gated on live, TCR-β+ cells are shown for CD4 (Left) or CD8 (Right). CD5 CV of 66.83 ± 2.91 in CD4 CD44hi; CD5 CV of 52.98 ± 0.69 in CD4 CD44lo; CD44 CV of 65.65 ± 1.24 in CD4 CD44hi; CD44 CV of 60.80 ± 2.35 in CD4 CD44lo. (K) Western blot analysis from B6 lymphocytes sorted on 20% CD5hi and CD5lo within CD4+ and CD8+ T cell (TCR-β+) populations. Representative Western blot is shown (n = 3 from 3 independent experiments). Sorting strategy is shown in SI Appendix, Fig. S1. (L) Peripheral T cells from WT animals were sorted on 20% CD5hi and CD5lo expression as indicated in SI Appendix, Fig. S1. Cells were lysed and RNA was harvested for reverse transcription. cDNA was analyzed for expression of Actb and Nfkbia between groups. Fold-change (2ΔΔCT) of CD5hi cells compared to CD5lo cells of either CD4 or CD8 populations normalized to expression of Actb (n = 6 from 2 independent experiments).

anti cd5 - by Bioz Stars, 2023-03

86/100 stars

Images

1) Product Images from "CD5 dynamically calibrates basal NF-κB signaling in T cells during thymic development and peripheral activation"

Article Title: CD5 dynamically calibrates basal NF-κB signaling in T cells during thymic development and peripheral activation

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi: 10.1073/pnas.1922525117

Components of NF-κB signaling are selectively altered in CD5hi compared to CD5lo peripheral T cells. The surface levels of CD5 and intracellular levels of Zap70 (A), PLCγ (B), LAT (C), IκBα (D), or NF-κB (E), are shown for either CD4 (Left) or CD8 (Right) T cells. The gMFI of CD5 (F), IκBα (G), or NFκB (H) expressed by CD4 or CD8 T cells from lymph node or spleen cells, gated on live, TCR-β+ cells (n = 11 from 3 independent experiments). Statistical significance was calculated using a paired t test. ***P < 0.001; anything not marked is not statistically significant. (I) The surface levels of CD5 and CD44 are shown for CD4 (Left) or CD8 (Right) T cells. (J) The distribution of IκBα expression with other cell surface markers (CD127, CD44, CD62L, TCR-β) on polyclonal peripheral T cells from lymph node or spleen gated on live, TCR-β+ cells are shown for CD4 (Left) or CD8 (Right). CD5 CV of 66.83 ± 2.91 in CD4 CD44hi; CD5 CV of 52.98 ± 0.69 in CD4 CD44lo; CD44 CV of 65.65 ± 1.24 in CD4 CD44hi; CD44 CV of 60.80 ± 2.35 in CD4 CD44lo. (K) Western blot analysis from B6 lymphocytes sorted on 20% CD5hi and CD5lo within CD4+ and CD8+ T cell (TCR-β+) populations. Representative Western blot is shown (n = 3 from 3 independent experiments). Sorting strategy is shown in SI Appendix, Fig. S1. (L) Peripheral T cells from WT animals were sorted on 20% CD5hi and CD5lo expression as indicated in SI Appendix, Fig. S1. Cells were lysed and RNA was harvested for reverse transcription. cDNA was analyzed for expression of Actb and Nfkbia between groups. Fold-change (2ΔΔCT) of CD5hi cells compared to CD5lo cells of either CD4 or CD8 populations normalized to expression of Actb (n = 6 from 2 independent experiments).

Figure Legend Snippet: Components of NF-κB signaling are selectively altered in CD5hi compared to CD5lo peripheral T cells. The surface levels of CD5 and intracellular levels of Zap70 (A), PLCγ (B), LAT (C), IκBα (D), or NF-κB (E), are shown for either CD4 (Left) or CD8 (Right) T cells. The gMFI of CD5 (F), IκBα (G), or NFκB (H) expressed by CD4 or CD8 T cells from lymph node or spleen cells, gated on live, TCR-β+ cells (n = 11 from 3 independent experiments). Statistical significance was calculated using a paired t test. ***P < 0.001; anything not marked is not statistically significant. (I) The surface levels of CD5 and CD44 are shown for CD4 (Left) or CD8 (Right) T cells. (J) The distribution of IκBα expression with other cell surface markers (CD127, CD44, CD62L, TCR-β) on polyclonal peripheral T cells from lymph node or spleen gated on live, TCR-β+ cells are shown for CD4 (Left) or CD8 (Right). CD5 CV of 66.83 ± 2.91 in CD4 CD44hi; CD5 CV of 52.98 ± 0.69 in CD4 CD44lo; CD44 CV of 65.65 ± 1.24 in CD4 CD44hi; CD44 CV of 60.80 ± 2.35 in CD4 CD44lo. (K) Western blot analysis from B6 lymphocytes sorted on 20% CD5hi and CD5lo within CD4+ and CD8+ T cell (TCR-β+) populations. Representative Western blot is shown (n = 3 from 3 independent experiments). Sorting strategy is shown in SI Appendix, Fig. S1. (L) Peripheral T cells from WT animals were sorted on 20% CD5hi and CD5lo expression as indicated in SI Appendix, Fig. S1. Cells were lysed and RNA was harvested for reverse transcription. cDNA was analyzed for expression of Actb and Nfkbia between groups. Fold-change (2ΔΔCT) of CD5hi cells compared to CD5lo cells of either CD4 or CD8 populations normalized to expression of Actb (n = 6 from 2 independent experiments).

Techniques Used: Expressing, Western Blot

Concomitant up-regulation of IκBα with developmental and peripheral modulation of CD5 levels. (A) Representative plots showing the levels of CD5 versus IκBα progressive stages of T cell development in the thymus. Thymocytes were stained for CD4, CD8, and CD69 and gated on markers listed on top of each panel (gating strategy shown in SI Appendix, Fig. S2A). DP = CD4+CD8+ and SP = single positive for either CD4 or CD8 expression as designated. (B) The CD5 and IκBα gMFI on thymocyte subsets as identified in A (n = 11 from 3 independent experiments; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons). (C) Representative peripheral expression levels of CD5 and IκBα in T cells gated by CD4+, TCR-β+, and CD25 expression, to enrich for regulatory T cells in pooled lymph node (the CD25hi population). (D) The CD5 and IκBα gMFI on CD25hi and CD25lo T cells (n = 7 from 3 independent experiments; statistical significance was calculated using paired t tests). (E) 5C.C7 TCR-tg CD4+ T cells were adoptively transferred to congenically distinct B10.A mice and challenged with MCC peptide and LPS challenge in B10.A hosts. Expression of CD5 was analyzed at varying time points after challenge on adoptively transferred TCR-tg CD4+ T cells (n = 2 to 3 from 1 independent experiment; statistical significance was calculated using Tukey’s multiple comparisons). (F) Representative flow plots gated on live, TCR-β+ and CD4+ or CD8+ cells, further subdivided by CD44 expression (gating strategy is shown in SI Appendix, Fig. S2). (G) CD5 and IκBα gMFI on CD4 or CD8 cells gated as indicated in F from lymph node or spleen (n = 11 from 3 independent experiments; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Anything not marked is not statistically significant.

Figure Legend Snippet: Concomitant up-regulation of IκBα with developmental and peripheral modulation of CD5 levels. (A) Representative plots showing the levels of CD5 versus IκBα progressive stages of T cell development in the thymus. Thymocytes were stained for CD4, CD8, and CD69 and gated on markers listed on top of each panel (gating strategy shown in SI Appendix, Fig. S2A). DP = CD4+CD8+ and SP = single positive for either CD4 or CD8 expression as designated. (B) The CD5 and IκBα gMFI on thymocyte subsets as identified in A (n = 11 from 3 independent experiments; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons). (C) Representative peripheral expression levels of CD5 and IκBα in T cells gated by CD4+, TCR-β+, and CD25 expression, to enrich for regulatory T cells in pooled lymph node (the CD25hi population). (D) The CD5 and IκBα gMFI on CD25hi and CD25lo T cells (n = 7 from 3 independent experiments; statistical significance was calculated using paired t tests). (E) 5C.C7 TCR-tg CD4+ T cells were adoptively transferred to congenically distinct B10.A mice and challenged with MCC peptide and LPS challenge in B10.A hosts. Expression of CD5 was analyzed at varying time points after challenge on adoptively transferred TCR-tg CD4+ T cells (n = 2 to 3 from 1 independent experiment; statistical significance was calculated using Tukey’s multiple comparisons). (F) Representative flow plots gated on live, TCR-β+ and CD4+ or CD8+ cells, further subdivided by CD44 expression (gating strategy is shown in SI Appendix, Fig. S2). (G) CD5 and IκBα gMFI on CD4 or CD8 cells gated as indicated in F from lymph node or spleen (n = 11 from 3 independent experiments; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Anything not marked is not statistically significant.

Techniques Used: Staining, Expressing

CD5-IκBα correlation is independent of SHP-1 and has a saturable level of IκBα up-regulation. (A) Western blot analysis of thymocytes from WT and SHP-1–KO animals (n = 1 from 1 independent experiment). (B) Flow cytometry analysis of WT and SHP-1–KO splenocytes (n = 1 from 1 independent experiment). (C) CD5 and IκBα gMFI in WT and SHP-1–KO splenocytes (n = 1 from 1 independent experiment). (D) CD5 and IκBα gMFI of peripheral T cells after 24 h ex vivo culture in the presence or absence of varying concentrations CX4945 (n = 3 from 1 independent experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). (E) WT and CD5 transgenic (CD5-Tg) thymocytes were harvested and analyzed for expression of CD5 and IκBα. Representative flow plots are shown. (F) CD5 and IκBα gMFI on peripheral CD4 and CD8 T cells from WT and CD5-Tg animals (n = 3 from 1 independent experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). (G) WT and CD5-Tg lymphocytes were harvested from pooled lymph nodes and grouped into bins by CD5 expression as detailed in SI Appendix, Fig. S5. Frequency of population in each bin is displayed for CD4 and CD8 peripheral T cells (n = 3 from 1 independent experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). (H) Lymphocytes from SMARTA CD4+ TCR-Tg mice were isolated and stimulated ex vivo with TCR-αβ KO antigen presenting cells and cognate peptide for the indicated time points. Cells were harvested and analyzed by flow cytometry. CD5 and IκBα gMFI of SMARTA CD4+ TCR-Tg T cells (n = 1 from 1 independent experiment). (I) BW-5147 cells were transduced with empty or CD5 retroviral vector. Cells were rested for 5 d after transduction and then analyzed by flow cytometry. Representative flow plots are shown. (J) CD5 and IκBα gMFI on retrovirally transduced BW-5147 cells (n = 4 from 3 independent experiments; statistical significance was calculated using one-way ANOVA with Tukey’s multiple comparisons test). **P < 0.01. (K) 3T3 cells were transduced with empty retroviral vector or with CD5 retroviral vector. Cells were rested for 48 h after transduction and then analyzed by flow cytometry. (n = 1 from 1 independent experiment).

Figure Legend Snippet: CD5-IκBα correlation is independent of SHP-1 and has a saturable level of IκBα up-regulation. (A) Western blot analysis of thymocytes from WT and SHP-1–KO animals (n = 1 from 1 independent experiment). (B) Flow cytometry analysis of WT and SHP-1–KO splenocytes (n = 1 from 1 independent experiment). (C) CD5 and IκBα gMFI in WT and SHP-1–KO splenocytes (n = 1 from 1 independent experiment). (D) CD5 and IκBα gMFI of peripheral T cells after 24 h ex vivo culture in the presence or absence of varying concentrations CX4945 (n = 3 from 1 independent experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). (E) WT and CD5 transgenic (CD5-Tg) thymocytes were harvested and analyzed for expression of CD5 and IκBα. Representative flow plots are shown. (F) CD5 and IκBα gMFI on peripheral CD4 and CD8 T cells from WT and CD5-Tg animals (n = 3 from 1 independent experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). (G) WT and CD5-Tg lymphocytes were harvested from pooled lymph nodes and grouped into bins by CD5 expression as detailed in SI Appendix, Fig. S5. Frequency of population in each bin is displayed for CD4 and CD8 peripheral T cells (n = 3 from 1 independent experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). (H) Lymphocytes from SMARTA CD4+ TCR-Tg mice were isolated and stimulated ex vivo with TCR-αβ KO antigen presenting cells and cognate peptide for the indicated time points. Cells were harvested and analyzed by flow cytometry. CD5 and IκBα gMFI of SMARTA CD4+ TCR-Tg T cells (n = 1 from 1 independent experiment). (I) BW-5147 cells were transduced with empty or CD5 retroviral vector. Cells were rested for 5 d after transduction and then analyzed by flow cytometry. Representative flow plots are shown. (J) CD5 and IκBα gMFI on retrovirally transduced BW-5147 cells (n = 4 from 3 independent experiments; statistical significance was calculated using one-way ANOVA with Tukey’s multiple comparisons test). **P < 0.01. (K) 3T3 cells were transduced with empty retroviral vector or with CD5 retroviral vector. Cells were rested for 48 h after transduction and then analyzed by flow cytometry. (n = 1 from 1 independent experiment).

Techniques Used: Western Blot, Flow Cytometry, Ex Vivo, Transgenic Assay, Expressing, Isolation, Transduction, Plasmid Preparation

The surface expression of CD5 is necessary for maintaining heterogenous levels of IκBα in T cells. (A) A similar gating strategy as in Fig. 2A was used to examine different thymocyte subsets in WT (Upper) or CD5-KO (Lower) mice and representative flow plots are shown. (B) The gMFI of CD5 and IκBα on thymocyte populations as gated in Fig. 2A from WT or CD5-KO mice (n = 3 to 5 from 1 experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). (C) Peripheral live, TCR-β+ CD4, or CD8 T cells from the pooled lymph nodes of WT or CD5-KO mice were analyzed for CD5 and IκBα expression and a representative set of flow plots are shown. (D) The gMFI of CD5 and IκBα gMFI in peripheral CD4 or CD8 T cells gated as indicated above from lymph nodes, respectively (n = 3 to 5 from 1 experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). (E) Representative flow plots of thymocytes and peripheral T cells isolated from lymph nodes of CD5-floxed mice with CAG-CreERT2 transgene (CD5 inducible knockouts; CD5-iKO) after treatment with tamoxifen for 5 d. Histograms of CD5 expression are shown. (F) The expression of CD5 and IκBα in thymocytes of either wild-type (WT, Upper) or CD5-floxed mice with CAG-CreERT2 transgene (CD5-iKO, Lower) after treatment with tamoxifen for 5 d. Representative flow plots are shown. (G) CD5 and IκBα gMFI on thymocytes as gated in Fig. 2A from WT or CD5-iKO mice (n = 2 to 5 from 1 experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). (H) The expression of CD5 and IκBα in peripheral T cells in either WT or CD5-iKO mice after treatment with tamoxifen for 5 d. Representative flow plots are shown. (I) CD5 and IκBα of peripheral CD4 or CD8 T cells gated as indicated above from lymph nodes of WT or CD5-iKO mice (n = 2 to 5 from 1 experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test).

Figure Legend Snippet: The surface expression of CD5 is necessary for maintaining heterogenous levels of IκBα in T cells. (A) A similar gating strategy as in Fig. 2A was used to examine different thymocyte subsets in WT (Upper) or CD5-KO (Lower) mice and representative flow plots are shown. (B) The gMFI of CD5 and IκBα on thymocyte populations as gated in Fig. 2A from WT or CD5-KO mice (n = 3 to 5 from 1 experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). (C) Peripheral live, TCR-β+ CD4, or CD8 T cells from the pooled lymph nodes of WT or CD5-KO mice were analyzed for CD5 and IκBα expression and a representative set of flow plots are shown. (D) The gMFI of CD5 and IκBα gMFI in peripheral CD4 or CD8 T cells gated as indicated above from lymph nodes, respectively (n = 3 to 5 from 1 experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). (E) Representative flow plots of thymocytes and peripheral T cells isolated from lymph nodes of CD5-floxed mice with CAG-CreERT2 transgene (CD5 inducible knockouts; CD5-iKO) after treatment with tamoxifen for 5 d. Histograms of CD5 expression are shown. (F) The expression of CD5 and IκBα in thymocytes of either wild-type (WT, Upper) or CD5-floxed mice with CAG-CreERT2 transgene (CD5-iKO, Lower) after treatment with tamoxifen for 5 d. Representative flow plots are shown. (G) CD5 and IκBα gMFI on thymocytes as gated in Fig. 2A from WT or CD5-iKO mice (n = 2 to 5 from 1 experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test). (H) The expression of CD5 and IκBα in peripheral T cells in either WT or CD5-iKO mice after treatment with tamoxifen for 5 d. Representative flow plots are shown. (I) CD5 and IκBα of peripheral CD4 or CD8 T cells gated as indicated above from lymph nodes of WT or CD5-iKO mice (n = 2 to 5 from 1 experiment; statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test).

Techniques Used: Expressing, Isolation

CD5 expression confers thymocyte survival advantage. (A) Thymocytes from B6 mice were cultured ex vivo for 24 h. Cells were stained and gated as in SI Appendix, Fig. S2A, together with Annexin V and 7AAD to assess cell death. The populations were grouped based on top and bottom 20% of CD5 expression within each population. Representative flow plots are shown. (B) The frequencies of Annexin V+ cells within the top and bottom 20% of CD5 expressers are shown (n = 4 from 1 experiment is shown and n = 3 from a second independent experiment). (C) Thymocytes from B6 mice were cultured ex vivo for 15 h with or without varying Bay 11-7082 doses. Cells were stained and gated as in SI Appendix, Fig. S2, together with Annexin V and 7AAD to assess cell death. Representative flow plots are shown for the double-positive CD69hi population. (D) The frequencies of Annexin V+ cells within the top and bottom 20% of CD5 expressers of the double-positive CD69hi population are shown (n = 3 from 1 independent experiment). *P < 0.05; **P < 0.01; ***P < 0.001; anything not marked is not statistically significant.

Figure Legend Snippet: CD5 expression confers thymocyte survival advantage. (A) Thymocytes from B6 mice were cultured ex vivo for 24 h. Cells were stained and gated as in SI Appendix, Fig. S2A, together with Annexin V and 7AAD to assess cell death. The populations were grouped based on top and bottom 20% of CD5 expression within each population. Representative flow plots are shown. (B) The frequencies of Annexin V+ cells within the top and bottom 20% of CD5 expressers are shown (n = 4 from 1 experiment is shown and n = 3 from a second independent experiment). (C) Thymocytes from B6 mice were cultured ex vivo for 15 h with or without varying Bay 11-7082 doses. Cells were stained and gated as in SI Appendix, Fig. S2, together with Annexin V and 7AAD to assess cell death. Representative flow plots are shown for the double-positive CD69hi population. (D) The frequencies of Annexin V+ cells within the top and bottom 20% of CD5 expressers of the double-positive CD69hi population are shown (n = 3 from 1 independent experiment). *P < 0.05; **P < 0.01; ***P < 0.001; anything not marked is not statistically significant.

Techniques Used: Expressing, Cell Culture, Ex Vivo, Staining

anti cd5 (Santa Cruz Biotechnology)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Santa Cruz Biotechnology anti cd5

anti cd5 - by Bioz Stars, 2023-03

86/100 stars

Images

1) Product Images from "CD5 dynamically calibrates basal NF-κB signaling in T cells during thymic development and peripheral activation"

Article Title: CD5 dynamically calibrates basal NF-κB signaling in T cells during thymic development and peripheral activation

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi: 10.1073/pnas.1922525117

Techniques Used: Expressing, Western Blot

Techniques Used: Staining, Expressing

Techniques Used: Western Blot, Flow Cytometry, Ex Vivo, Transgenic Assay, Expressing, Isolation, Transduction, Plasmid Preparation

Techniques Used: Expressing, Isolation

Techniques Used: Expressing, Cell Culture, Ex Vivo, Staining

cd5 pe (Santa Cruz Biotechnology)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Santa Cruz Biotechnology cd5 pe

Infiltrating T cells (CD3, <t>CD5),</t> B cells (CD 20) and macrophages [CD68], are increased in the renal cortical interstitium of the angiotensin-infused rats (Ang II group n = 7), and reduced by the administration of BBG (Ang II + BBG n = 7). CD45 antigen is a leukocyte common antigen predominantly expressed in lymphocytes. White bars (□) Sham; Black bars (■) AngII; and grey bars ( ■ ) Ang II + BBG. * p < 0.001 vs. sham, ** p < 0.05 vs. Ang II. Ang II = angiotensin II; BBG = brilliant blue G.

Cd5 Pe, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd5 pe/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

cd5 pe - by Bioz Stars, 2023-03

86/100 stars

Images

1) Product Images from "The Role of P2X7 Purinergic Receptors in the Renal Inflammation Associated with Angiotensin II-Induced Hypertension"

Article Title: The Role of P2X7 Purinergic Receptors in the Renal Inflammation Associated with Angiotensin II-Induced Hypertension

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms21114041

Infiltrating T cells (CD3, CD5), B cells (CD 20) and macrophages [CD68], are increased in the renal cortical interstitium of the angiotensin-infused rats (Ang II group n = 7), and reduced by the administration of BBG (Ang II + BBG n = 7). CD45 antigen is a leukocyte common antigen predominantly expressed in lymphocytes. White bars (□) Sham; Black bars (■) AngII; and grey bars ( ■ ) Ang II + BBG. * p < 0.001 vs. sham, ** p < 0.05 vs. Ang II. Ang II = angiotensin II; BBG = brilliant blue G.

Figure Legend Snippet: Infiltrating T cells (CD3, CD5), B cells (CD 20) and macrophages [CD68], are increased in the renal cortical interstitium of the angiotensin-infused rats (Ang II group n = 7), and reduced by the administration of BBG (Ang II + BBG n = 7). CD45 antigen is a leukocyte common antigen predominantly expressed in lymphocytes. White bars (□) Sham; Black bars (■) AngII; and grey bars ( ■ ) Ang II + BBG. * p < 0.001 vs. sham, ** p < 0.05 vs. Ang II. Ang II = angiotensin II; BBG = brilliant blue G.

Techniques Used:

P2X7 receptors (second column) in the infiltrating T cells of Ang II group co-localize with cluster differentiation (CD, first column) antigens in T cells (CD 3 positive cells), B cells (CD5 and CD20 positive cells), macrophages (CD68 positive cells) and leukocyte common antigen CD45, predominantly expressed in T lymphocytes.

Figure Legend Snippet: P2X7 receptors (second column) in the infiltrating T cells of Ang II group co-localize with cluster differentiation (CD, first column) antigens in T cells (CD 3 positive cells), B cells (CD5 and CD20 positive cells), macrophages (CD68 positive cells) and leukocyte common antigen CD45, predominantly expressed in T lymphocytes.

Techniques Used:

cd5 (Santa Cruz Biotechnology)

Structured Review

Images

1) Product Images from "Modulating Oxidative Stress in B Cells Promotes Immunotherapy in Food Allergy"

cd5 (Santa Cruz Biotechnology)

Structured Review

Images

1) Product Images from "A retrospective study of 18 children with subcutaneous panniculitis-like T-cell lymphoma: multidrug combination chemotherapy or immunomodulatory therapy?"

cd5 (Santa Cruz Biotechnology)

Structured Review

Images

cd5 (Santa Cruz Biotechnology)

Structured Review

Images

1) Product Images from "Propionic acid regulates immune tolerant properties in B Cells"

anti cd5 (Santa Cruz Biotechnology)

Structured Review

Images

cd5 (Santa Cruz Biotechnology)

Structured Review

Images

anti cd5 (Santa Cruz Biotechnology)

Structured Review

Images

1) Product Images from "Dermatan Sulfate Is a Potential Master Regulator of IgH via Interactions with Pre-BCR, GTF2I, and BiP ER Complex in Pre-B Lymphoblasts"

anti cd5 (Santa Cruz Biotechnology)

Structured Review

Images

1) Product Images from "CD5 dynamically calibrates basal NF-κB signaling in T cells during thymic development and peripheral activation"

anti cd5 (Santa Cruz Biotechnology)

Structured Review

Images

1) Product Images from "CD5 dynamically calibrates basal NF-κB signaling in T cells during thymic development and peripheral activation"

cd5 pe (Santa Cruz Biotechnology)

Structured Review

Images

1) Product Images from "The Role of P2X7 Purinergic Receptors in the Renal Inflammation Associated with Angiotensin II-Induced Hypertension"

Similar Products

Image Search Results