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Analysis of cell population in tumor of intact, SE and SE+ST animals on Days 7 (A), 12 (B) and 15 (C). Blue arrows—gating from CD3+ cell population, green arrows—gating from CD3− cell population. D, Percentage content of CD3+, CD8a+, CD4+, <t>CD45R+,</t> F4/80+Ly6C−, F4/80−Ly6C+, F4/80+Ly6C+ cells. (A–D) N = 3 per group. Statistical analysis was carried out by non‐parametric Kruskal–Wallis test with post hoc Dunn's test (mean with SEM), * р ‐value ≤ 0.05, ** p ‐value ≤ 0.005, *** p ‐value ≤ 0.0005.
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Analysis of cell population in tumor of intact, SE and SE+ST animals on Days 7 (A), 12 (B) and 15 (C). Blue arrows—gating from CD3+ cell population, green arrows—gating from CD3− cell population. D, Percentage content of CD3+, CD8a+, CD4+, <t>CD45R+,</t> F4/80+Ly6C−, F4/80−Ly6C+, F4/80+Ly6C+ cells. (A–D) N = 3 per group. Statistical analysis was carried out by non‐parametric Kruskal–Wallis test with post hoc Dunn's test (mean with SEM), * р ‐value ≤ 0.05, ** p ‐value ≤ 0.005, *** p ‐value ≤ 0.0005.
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Analysis of cell population in tumor of intact, SE and SE+ST animals on Days 7 (A), 12 (B) and 15 (C). Blue arrows—gating from CD3+ cell population, green arrows—gating from CD3− cell population. D, Percentage content of CD3+, CD8a+, CD4+, <t>CD45R+,</t> F4/80+Ly6C−, F4/80−Ly6C+, F4/80+Ly6C+ cells. (A–D) N = 3 per group. Statistical analysis was carried out by non‐parametric Kruskal–Wallis test with post hoc Dunn's test (mean with SEM), * р ‐value ≤ 0.05, ** p ‐value ≤ 0.005, *** p ‐value ≤ 0.0005.
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Analysis of cell population in tumor of intact, SE and SE+ST animals on Days 7 (A), 12 (B) and 15 (C). Blue arrows—gating from CD3+ cell population, green arrows—gating from CD3− cell population. D, Percentage content of CD3+, CD8a+, CD4+, <t>CD45R+,</t> F4/80+Ly6C−, F4/80−Ly6C+, F4/80+Ly6C+ cells. (A–D) N = 3 per group. Statistical analysis was carried out by non‐parametric Kruskal–Wallis test with post hoc Dunn's test (mean with SEM), * р ‐value ≤ 0.05, ** p ‐value ≤ 0.005, *** p ‐value ≤ 0.0005.
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Analysis of cell population in tumor of intact, SE and SE+ST animals on Days 7 (A), 12 (B) and 15 (C). Blue arrows—gating from CD3+ cell population, green arrows—gating from CD3− cell population. D, Percentage content of CD3+, CD8a+, CD4+, CD45R+, F4/80+Ly6C−, F4/80−Ly6C+, F4/80+Ly6C+ cells. (A–D) N = 3 per group. Statistical analysis was carried out by non‐parametric Kruskal–Wallis test with post hoc Dunn's test (mean with SEM), * р ‐value ≤ 0.05, ** p ‐value ≤ 0.005, *** p ‐value ≤ 0.0005.

Journal: FASEB BioAdvances

Article Title: The Impact of Heterotopic Spleen Regeneration on Tumor Growth

doi: 10.1096/fba.2025-00254

Figure Lengend Snippet: Analysis of cell population in tumor of intact, SE and SE+ST animals on Days 7 (A), 12 (B) and 15 (C). Blue arrows—gating from CD3+ cell population, green arrows—gating from CD3− cell population. D, Percentage content of CD3+, CD8a+, CD4+, CD45R+, F4/80+Ly6C−, F4/80−Ly6C+, F4/80+Ly6C+ cells. (A–D) N = 3 per group. Statistical analysis was carried out by non‐parametric Kruskal–Wallis test with post hoc Dunn's test (mean with SEM), * р ‐value ≤ 0.05, ** p ‐value ≤ 0.005, *** p ‐value ≤ 0.0005.

Article Snippet: To determine the phenotypes of tumor‐infiltrating cells, isolated cells were stained for 1 h at room temperature in the dark with the following panel of anti‐mouse antibodies: CD8a antibody anti‐mouse VioBlue (130‐123‐865, Miltenyi Biotec, Germany), CD3 antibody anti‐mouse FITC, REAfinity (130‐119‐758, Miltenyi Biotec, Germany), F4/80‐PE REAfinity (130‐102‐422, Miltenyi Biotec, Germany), rat anti‐mouse CD4 StarBright Blue 700 (SBB700) (MCA2691SBB700, Biorad, USA), CD45R antibody anti‐mouse PE‐Vio 770 (130‐102‐817, Miltenyi Biotec, Germany), Ly‐6C antibody anti‐mouse APC REAfinity (130‐111‐779, Miltenyi Biotec, Germany).

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