apoptosis signaling cascade anti cd45 polyclonal igg rabbit  (Proteintech)

 
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    Structured Review

    Proteintech apoptosis signaling cascade anti cd45 polyclonal igg rabbit
    Apoptosis Signaling Cascade Anti Cd45 Polyclonal Igg Rabbit, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoptosis signaling cascade anti cd45 polyclonal igg rabbit/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apoptosis signaling cascade anti cd45 polyclonal igg rabbit - by Bioz Stars, 2024-04
    86/100 stars

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    Proteintech rabbit polyclonal anti cd45
    a – j Zbp1 −/− and wild-type mice were divided into three groups: Ad5-hACE2 non-transduced and non-infected ( n = 3), transduced but non-infected ( n = 3), transduced and infected ( n = 6). Mice were intranasally transduced with Ad5-hACE2 (2.5 × 10 8 PFU) and infected with SARS-CoV-2 (1 × 10 5 TCID 50 ) at 5 days post transduction. Lung samples were harvested at 2 dpi. a Relative level of indicated genes was analyzed with qRT-PCR and normalized to non-transduced and uninfected wild-type controls. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t- test. ** P < 0.01; ns, no significance. b The histopathological changes in lung were evaluated by H&E staining. Scale bars, 1000 μm and 50 μm in enlarged image. Inflammatory cell infiltration (green arrow) and alveolar septa expansion (red arrow) were observed in the lung sections. Immunostaining of <t>CD45</t> ( c ), CD68 ( e ), MPO ( g ) and CD3 ( i ) representing the infiltration of leukocytes, macrophages, neutrophils and T cells, respectively. c , i Scale bars, 50 μm and 10 μm in the enlarged image. e , g Scale bars, 50 μm. d , f , h , j Quantitative analysis of positive cells was performed with ImageJ for c , e , g , i , respectively. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t -test. ** P < 0.01; **** P < 0.0001.
    Rabbit Polyclonal Anti Cd45, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti cd45/product/Proteintech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti cd45 - by Bioz Stars, 2024-04
    94/100 stars

    Images

    1) Product Images from "SARS-CoV-2 Z-RNA activates the ZBP1-RIPK3 pathway to promote virus-induced inflammatory responses"

    Article Title: SARS-CoV-2 Z-RNA activates the ZBP1-RIPK3 pathway to promote virus-induced inflammatory responses

    Journal: Cell Research

    doi: 10.1038/s41422-022-00775-y

    a – j Zbp1 −/− and wild-type mice were divided into three groups: Ad5-hACE2 non-transduced and non-infected ( n = 3), transduced but non-infected ( n = 3), transduced and infected ( n = 6). Mice were intranasally transduced with Ad5-hACE2 (2.5 × 10 8 PFU) and infected with SARS-CoV-2 (1 × 10 5 TCID 50 ) at 5 days post transduction. Lung samples were harvested at 2 dpi. a Relative level of indicated genes was analyzed with qRT-PCR and normalized to non-transduced and uninfected wild-type controls. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t- test. ** P < 0.01; ns, no significance. b The histopathological changes in lung were evaluated by H&E staining. Scale bars, 1000 μm and 50 μm in enlarged image. Inflammatory cell infiltration (green arrow) and alveolar septa expansion (red arrow) were observed in the lung sections. Immunostaining of CD45 ( c ), CD68 ( e ), MPO ( g ) and CD3 ( i ) representing the infiltration of leukocytes, macrophages, neutrophils and T cells, respectively. c , i Scale bars, 50 μm and 10 μm in the enlarged image. e , g Scale bars, 50 μm. d , f , h , j Quantitative analysis of positive cells was performed with ImageJ for c , e , g , i , respectively. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t -test. ** P < 0.01; **** P < 0.0001.
    Figure Legend Snippet: a – j Zbp1 −/− and wild-type mice were divided into three groups: Ad5-hACE2 non-transduced and non-infected ( n = 3), transduced but non-infected ( n = 3), transduced and infected ( n = 6). Mice were intranasally transduced with Ad5-hACE2 (2.5 × 10 8 PFU) and infected with SARS-CoV-2 (1 × 10 5 TCID 50 ) at 5 days post transduction. Lung samples were harvested at 2 dpi. a Relative level of indicated genes was analyzed with qRT-PCR and normalized to non-transduced and uninfected wild-type controls. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t- test. ** P < 0.01; ns, no significance. b The histopathological changes in lung were evaluated by H&E staining. Scale bars, 1000 μm and 50 μm in enlarged image. Inflammatory cell infiltration (green arrow) and alveolar septa expansion (red arrow) were observed in the lung sections. Immunostaining of CD45 ( c ), CD68 ( e ), MPO ( g ) and CD3 ( i ) representing the infiltration of leukocytes, macrophages, neutrophils and T cells, respectively. c , i Scale bars, 50 μm and 10 μm in the enlarged image. e , g Scale bars, 50 μm. d , f , h , j Quantitative analysis of positive cells was performed with ImageJ for c , e , g , i , respectively. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t -test. ** P < 0.01; **** P < 0.0001.

    Techniques Used: Infection, Transduction, Quantitative RT-PCR, Staining, Immunostaining

    a – e Ad5-hACE2 (2.5 × 10 8 PFU) transduced Ripk3 −/− , Mlkl −/− or wild-type C57BL/6 mice were infected with SARS-CoV-2 (1 × 10 5 TCID 50 ). Lung samples were harvested at 2 dpi. a The histopathological changes were evaluated by H&E staining. Inflammatory cell infiltration (green arrow) and alveolar septa expansion (red arrow) were indicated. Scale bars, 1000 μm in the original image and 50 μm in the enlarged image. b – j Immunostaining of CD45 ( b ), CD68 ( d ), CD3 ( f ) and MPO ( h ) representing the infiltration of leukocytes, macrophages, T cells and neutrophils respectively. b , f Scale bars, 50 μm and 10 μm in the enlarged image. d , h Scale bars, 50 μm. c , e , g , i Quantitative analysis of positive cells performed with ImageJ for b , d , f , h , respectively. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t -test. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no significance. j Co-staining of CD8 (red) and CXCR3 (green) representing the recruitment of CD8 + CXCR3 + T cells in the lung section of SARS-CoV-2-infected wild-type and Ripk3 −/− mice. Scale bars, 10 μm.
    Figure Legend Snippet: a – e Ad5-hACE2 (2.5 × 10 8 PFU) transduced Ripk3 −/− , Mlkl −/− or wild-type C57BL/6 mice were infected with SARS-CoV-2 (1 × 10 5 TCID 50 ). Lung samples were harvested at 2 dpi. a The histopathological changes were evaluated by H&E staining. Inflammatory cell infiltration (green arrow) and alveolar septa expansion (red arrow) were indicated. Scale bars, 1000 μm in the original image and 50 μm in the enlarged image. b – j Immunostaining of CD45 ( b ), CD68 ( d ), CD3 ( f ) and MPO ( h ) representing the infiltration of leukocytes, macrophages, T cells and neutrophils respectively. b , f Scale bars, 50 μm and 10 μm in the enlarged image. d , h Scale bars, 50 μm. c , e , g , i Quantitative analysis of positive cells performed with ImageJ for b , d , f , h , respectively. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t -test. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no significance. j Co-staining of CD8 (red) and CXCR3 (green) representing the recruitment of CD8 + CXCR3 + T cells in the lung section of SARS-CoV-2-infected wild-type and Ripk3 −/− mice. Scale bars, 10 μm.

    Techniques Used: Infection, Staining, Immunostaining


    Structured Review

    Proteintech cd45 rabbit polyclonal antibody
    Cd45 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd45 rabbit polyclonal antibody/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd45 rabbit polyclonal antibody - by Bioz Stars, 2024-04
    86/100 stars

    Images


    Structured Review

    Proteintech cd45 rabbit polyclonal antibody
    Cd45 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd45 rabbit polyclonal antibody/product/Proteintech
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd45 rabbit polyclonal antibody - by Bioz Stars, 2024-04
    80/100 stars

    Images


    Structured Review

    Proteintech rabbit polyclonal cd45
    Rabbit Polyclonal Cd45, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal cd45/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal cd45 - by Bioz Stars, 2024-04
    86/100 stars

    Images


    Structured Review

    Proteintech rabbit cd45 polyclonal antibody
    Disease‐related transcriptomic changes in EC‐KO. (A) IHC staining of Aβ, <t>CD45,</t> Iba1, and CD147 in 18‐month‐old APP/PS1 mouse brain slices and peer comparisons. Scale bar: 100 μm (B) The expression of DEGs whose human orthologs are related to neurodegenerative and cerebrovascular diseases in GWAS datasets, expression is represented as FPKM. Disease abbreviations: ALS, amyotrophic lateral sclerosis; WMH burden/SVD, white matter hyperintensity burden and cerebral small vessel disease; MSA, multiple systems atrophy; AD, Alzheimer's disease; PD, Parkinson's disease; FTD, frontotemporal dementia; HD, Huntington's disease. There were 21 DEGs related to AD ( p = 0.00266). (C) Visual PPI network of the AD‐related dataset. The edges between nodes represent different protein–protein associations, including experimental determination (purple), interactions from current databases (blue), and coexpression (black). (D) Expression changes of selected genes (App, Psen2, Epha1, Cd2ap, Bin1, Mef2c, Scarb2) by RT‐qPCR. Data are presented as the mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. (E) Human AD brain differential expression versus the control ( p < 0.05) served as the y‐axis, and DEGs (fold change ≥2 with FDR‐adjusted p < 0.05) from EC‐KO mice versus WT mice served as the x‐axis. The genes and their human orthologs were concordant in the first and third quadrants (light green), whereas they were discordant in the second and fourth quadrants (light red). Twenty‐one DEGs were specific in the groups compared at 8–12 weeks of age (red dot, EC‐KO versus WT), and 35 DEGs were specific in the groups compared at 6–7 months of age (green dot), whereas 11 DEGs were shared (blue dot) in both groups
    Rabbit Cd45 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit cd45 polyclonal antibody/product/Proteintech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit cd45 polyclonal antibody - by Bioz Stars, 2024-04
    94/100 stars

    Images

    1) Product Images from "Endothelial genetic deletion of CD147 induces changes in the dual function of the blood‐brain barrier and is implicated in Alzheimer’s disease"

    Article Title: Endothelial genetic deletion of CD147 induces changes in the dual function of the blood‐brain barrier and is implicated in Alzheimer’s disease

    Journal: CNS Neuroscience & Therapeutics

    doi: 10.1111/cns.13659

    Disease‐related transcriptomic changes in EC‐KO. (A) IHC staining of Aβ, CD45, Iba1, and CD147 in 18‐month‐old APP/PS1 mouse brain slices and peer comparisons. Scale bar: 100 μm (B) The expression of DEGs whose human orthologs are related to neurodegenerative and cerebrovascular diseases in GWAS datasets, expression is represented as FPKM. Disease abbreviations: ALS, amyotrophic lateral sclerosis; WMH burden/SVD, white matter hyperintensity burden and cerebral small vessel disease; MSA, multiple systems atrophy; AD, Alzheimer's disease; PD, Parkinson's disease; FTD, frontotemporal dementia; HD, Huntington's disease. There were 21 DEGs related to AD ( p = 0.00266). (C) Visual PPI network of the AD‐related dataset. The edges between nodes represent different protein–protein associations, including experimental determination (purple), interactions from current databases (blue), and coexpression (black). (D) Expression changes of selected genes (App, Psen2, Epha1, Cd2ap, Bin1, Mef2c, Scarb2) by RT‐qPCR. Data are presented as the mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. (E) Human AD brain differential expression versus the control ( p < 0.05) served as the y‐axis, and DEGs (fold change ≥2 with FDR‐adjusted p < 0.05) from EC‐KO mice versus WT mice served as the x‐axis. The genes and their human orthologs were concordant in the first and third quadrants (light green), whereas they were discordant in the second and fourth quadrants (light red). Twenty‐one DEGs were specific in the groups compared at 8–12 weeks of age (red dot, EC‐KO versus WT), and 35 DEGs were specific in the groups compared at 6–7 months of age (green dot), whereas 11 DEGs were shared (blue dot) in both groups
    Figure Legend Snippet: Disease‐related transcriptomic changes in EC‐KO. (A) IHC staining of Aβ, CD45, Iba1, and CD147 in 18‐month‐old APP/PS1 mouse brain slices and peer comparisons. Scale bar: 100 μm (B) The expression of DEGs whose human orthologs are related to neurodegenerative and cerebrovascular diseases in GWAS datasets, expression is represented as FPKM. Disease abbreviations: ALS, amyotrophic lateral sclerosis; WMH burden/SVD, white matter hyperintensity burden and cerebral small vessel disease; MSA, multiple systems atrophy; AD, Alzheimer's disease; PD, Parkinson's disease; FTD, frontotemporal dementia; HD, Huntington's disease. There were 21 DEGs related to AD ( p = 0.00266). (C) Visual PPI network of the AD‐related dataset. The edges between nodes represent different protein–protein associations, including experimental determination (purple), interactions from current databases (blue), and coexpression (black). (D) Expression changes of selected genes (App, Psen2, Epha1, Cd2ap, Bin1, Mef2c, Scarb2) by RT‐qPCR. Data are presented as the mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. (E) Human AD brain differential expression versus the control ( p < 0.05) served as the y‐axis, and DEGs (fold change ≥2 with FDR‐adjusted p < 0.05) from EC‐KO mice versus WT mice served as the x‐axis. The genes and their human orthologs were concordant in the first and third quadrants (light green), whereas they were discordant in the second and fourth quadrants (light red). Twenty‐one DEGs were specific in the groups compared at 8–12 weeks of age (red dot, EC‐KO versus WT), and 35 DEGs were specific in the groups compared at 6–7 months of age (green dot), whereas 11 DEGs were shared (blue dot) in both groups

    Techniques Used: Immunohistochemistry, Expressing, Quantitative RT-PCR


    Structured Review

    Proteintech cd45 rabbit polyclonal antibody
    Cd45 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd45 rabbit polyclonal antibody/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd45 rabbit polyclonal antibody - by Bioz Stars, 2024-04
    86/100 stars

    Images


    Structured Review

    Proteintech cd45 rabbit polyclonal antibody
    Cd45 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd45 rabbit polyclonal antibody/product/Proteintech
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd45 rabbit polyclonal antibody - by Bioz Stars, 2024-04
    80/100 stars

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    Proteintech apoptosis signaling cascade anti cd45 polyclonal igg rabbit
    Apoptosis Signaling Cascade Anti Cd45 Polyclonal Igg Rabbit, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoptosis signaling cascade anti cd45 polyclonal igg rabbit/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apoptosis signaling cascade anti cd45 polyclonal igg rabbit - by Bioz Stars, 2024-04
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    94
    Proteintech rabbit polyclonal anti cd45
    a – j Zbp1 −/− and wild-type mice were divided into three groups: Ad5-hACE2 non-transduced and non-infected ( n = 3), transduced but non-infected ( n = 3), transduced and infected ( n = 6). Mice were intranasally transduced with Ad5-hACE2 (2.5 × 10 8 PFU) and infected with SARS-CoV-2 (1 × 10 5 TCID 50 ) at 5 days post transduction. Lung samples were harvested at 2 dpi. a Relative level of indicated genes was analyzed with qRT-PCR and normalized to non-transduced and uninfected wild-type controls. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t- test. ** P < 0.01; ns, no significance. b The histopathological changes in lung were evaluated by H&E staining. Scale bars, 1000 μm and 50 μm in enlarged image. Inflammatory cell infiltration (green arrow) and alveolar septa expansion (red arrow) were observed in the lung sections. Immunostaining of <t>CD45</t> ( c ), CD68 ( e ), MPO ( g ) and CD3 ( i ) representing the infiltration of leukocytes, macrophages, neutrophils and T cells, respectively. c , i Scale bars, 50 μm and 10 μm in the enlarged image. e , g Scale bars, 50 μm. d , f , h , j Quantitative analysis of positive cells was performed with ImageJ for c , e , g , i , respectively. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t -test. ** P < 0.01; **** P < 0.0001.
    Rabbit Polyclonal Anti Cd45, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti cd45/product/Proteintech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti cd45 - by Bioz Stars, 2024-04
    94/100 stars
      Buy from Supplier

    86
    Proteintech cd45 rabbit polyclonal antibody
    a – j Zbp1 −/− and wild-type mice were divided into three groups: Ad5-hACE2 non-transduced and non-infected ( n = 3), transduced but non-infected ( n = 3), transduced and infected ( n = 6). Mice were intranasally transduced with Ad5-hACE2 (2.5 × 10 8 PFU) and infected with SARS-CoV-2 (1 × 10 5 TCID 50 ) at 5 days post transduction. Lung samples were harvested at 2 dpi. a Relative level of indicated genes was analyzed with qRT-PCR and normalized to non-transduced and uninfected wild-type controls. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t- test. ** P < 0.01; ns, no significance. b The histopathological changes in lung were evaluated by H&E staining. Scale bars, 1000 μm and 50 μm in enlarged image. Inflammatory cell infiltration (green arrow) and alveolar septa expansion (red arrow) were observed in the lung sections. Immunostaining of <t>CD45</t> ( c ), CD68 ( e ), MPO ( g ) and CD3 ( i ) representing the infiltration of leukocytes, macrophages, neutrophils and T cells, respectively. c , i Scale bars, 50 μm and 10 μm in the enlarged image. e , g Scale bars, 50 μm. d , f , h , j Quantitative analysis of positive cells was performed with ImageJ for c , e , g , i , respectively. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t -test. ** P < 0.01; **** P < 0.0001.
    Cd45 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd45 rabbit polyclonal antibody/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd45 rabbit polyclonal antibody - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    Proteintech rabbit polyclonal cd45
    a – j Zbp1 −/− and wild-type mice were divided into three groups: Ad5-hACE2 non-transduced and non-infected ( n = 3), transduced but non-infected ( n = 3), transduced and infected ( n = 6). Mice were intranasally transduced with Ad5-hACE2 (2.5 × 10 8 PFU) and infected with SARS-CoV-2 (1 × 10 5 TCID 50 ) at 5 days post transduction. Lung samples were harvested at 2 dpi. a Relative level of indicated genes was analyzed with qRT-PCR and normalized to non-transduced and uninfected wild-type controls. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t- test. ** P < 0.01; ns, no significance. b The histopathological changes in lung were evaluated by H&E staining. Scale bars, 1000 μm and 50 μm in enlarged image. Inflammatory cell infiltration (green arrow) and alveolar septa expansion (red arrow) were observed in the lung sections. Immunostaining of <t>CD45</t> ( c ), CD68 ( e ), MPO ( g ) and CD3 ( i ) representing the infiltration of leukocytes, macrophages, neutrophils and T cells, respectively. c , i Scale bars, 50 μm and 10 μm in the enlarged image. e , g Scale bars, 50 μm. d , f , h , j Quantitative analysis of positive cells was performed with ImageJ for c , e , g , i , respectively. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t -test. ** P < 0.01; **** P < 0.0001.
    Rabbit Polyclonal Cd45, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal cd45/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal cd45 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    94
    Proteintech rabbit cd45 polyclonal antibody
    Disease‐related transcriptomic changes in EC‐KO. (A) IHC staining of Aβ, <t>CD45,</t> Iba1, and CD147 in 18‐month‐old APP/PS1 mouse brain slices and peer comparisons. Scale bar: 100 μm (B) The expression of DEGs whose human orthologs are related to neurodegenerative and cerebrovascular diseases in GWAS datasets, expression is represented as FPKM. Disease abbreviations: ALS, amyotrophic lateral sclerosis; WMH burden/SVD, white matter hyperintensity burden and cerebral small vessel disease; MSA, multiple systems atrophy; AD, Alzheimer's disease; PD, Parkinson's disease; FTD, frontotemporal dementia; HD, Huntington's disease. There were 21 DEGs related to AD ( p = 0.00266). (C) Visual PPI network of the AD‐related dataset. The edges between nodes represent different protein–protein associations, including experimental determination (purple), interactions from current databases (blue), and coexpression (black). (D) Expression changes of selected genes (App, Psen2, Epha1, Cd2ap, Bin1, Mef2c, Scarb2) by RT‐qPCR. Data are presented as the mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. (E) Human AD brain differential expression versus the control ( p < 0.05) served as the y‐axis, and DEGs (fold change ≥2 with FDR‐adjusted p < 0.05) from EC‐KO mice versus WT mice served as the x‐axis. The genes and their human orthologs were concordant in the first and third quadrants (light green), whereas they were discordant in the second and fourth quadrants (light red). Twenty‐one DEGs were specific in the groups compared at 8–12 weeks of age (red dot, EC‐KO versus WT), and 35 DEGs were specific in the groups compared at 6–7 months of age (green dot), whereas 11 DEGs were shared (blue dot) in both groups
    Rabbit Cd45 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit cd45 polyclonal antibody/product/Proteintech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit cd45 polyclonal antibody - by Bioz Stars, 2024-04
    94/100 stars
      Buy from Supplier

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    a – j Zbp1 −/− and wild-type mice were divided into three groups: Ad5-hACE2 non-transduced and non-infected ( n = 3), transduced but non-infected ( n = 3), transduced and infected ( n = 6). Mice were intranasally transduced with Ad5-hACE2 (2.5 × 10 8 PFU) and infected with SARS-CoV-2 (1 × 10 5 TCID 50 ) at 5 days post transduction. Lung samples were harvested at 2 dpi. a Relative level of indicated genes was analyzed with qRT-PCR and normalized to non-transduced and uninfected wild-type controls. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t- test. ** P < 0.01; ns, no significance. b The histopathological changes in lung were evaluated by H&E staining. Scale bars, 1000 μm and 50 μm in enlarged image. Inflammatory cell infiltration (green arrow) and alveolar septa expansion (red arrow) were observed in the lung sections. Immunostaining of CD45 ( c ), CD68 ( e ), MPO ( g ) and CD3 ( i ) representing the infiltration of leukocytes, macrophages, neutrophils and T cells, respectively. c , i Scale bars, 50 μm and 10 μm in the enlarged image. e , g Scale bars, 50 μm. d , f , h , j Quantitative analysis of positive cells was performed with ImageJ for c , e , g , i , respectively. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t -test. ** P < 0.01; **** P < 0.0001.

    Journal: Cell Research

    Article Title: SARS-CoV-2 Z-RNA activates the ZBP1-RIPK3 pathway to promote virus-induced inflammatory responses

    doi: 10.1038/s41422-022-00775-y

    Figure Lengend Snippet: a – j Zbp1 −/− and wild-type mice were divided into three groups: Ad5-hACE2 non-transduced and non-infected ( n = 3), transduced but non-infected ( n = 3), transduced and infected ( n = 6). Mice were intranasally transduced with Ad5-hACE2 (2.5 × 10 8 PFU) and infected with SARS-CoV-2 (1 × 10 5 TCID 50 ) at 5 days post transduction. Lung samples were harvested at 2 dpi. a Relative level of indicated genes was analyzed with qRT-PCR and normalized to non-transduced and uninfected wild-type controls. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t- test. ** P < 0.01; ns, no significance. b The histopathological changes in lung were evaluated by H&E staining. Scale bars, 1000 μm and 50 μm in enlarged image. Inflammatory cell infiltration (green arrow) and alveolar septa expansion (red arrow) were observed in the lung sections. Immunostaining of CD45 ( c ), CD68 ( e ), MPO ( g ) and CD3 ( i ) representing the infiltration of leukocytes, macrophages, neutrophils and T cells, respectively. c , i Scale bars, 50 μm and 10 μm in the enlarged image. e , g Scale bars, 50 μm. d , f , h , j Quantitative analysis of positive cells was performed with ImageJ for c , e , g , i , respectively. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t -test. ** P < 0.01; **** P < 0.0001.

    Article Snippet: Rabbit polyclonal anti-CD45 (20103-1-AP), anti-α-tubulin (11224-1-AP) and mouse IgG (B900620) were obtained from Proteintech (Chicago, IL, USA).

    Techniques: Infection, Transduction, Quantitative RT-PCR, Staining, Immunostaining

    a – e Ad5-hACE2 (2.5 × 10 8 PFU) transduced Ripk3 −/− , Mlkl −/− or wild-type C57BL/6 mice were infected with SARS-CoV-2 (1 × 10 5 TCID 50 ). Lung samples were harvested at 2 dpi. a The histopathological changes were evaluated by H&E staining. Inflammatory cell infiltration (green arrow) and alveolar septa expansion (red arrow) were indicated. Scale bars, 1000 μm in the original image and 50 μm in the enlarged image. b – j Immunostaining of CD45 ( b ), CD68 ( d ), CD3 ( f ) and MPO ( h ) representing the infiltration of leukocytes, macrophages, T cells and neutrophils respectively. b , f Scale bars, 50 μm and 10 μm in the enlarged image. d , h Scale bars, 50 μm. c , e , g , i Quantitative analysis of positive cells performed with ImageJ for b , d , f , h , respectively. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t -test. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no significance. j Co-staining of CD8 (red) and CXCR3 (green) representing the recruitment of CD8 + CXCR3 + T cells in the lung section of SARS-CoV-2-infected wild-type and Ripk3 −/− mice. Scale bars, 10 μm.

    Journal: Cell Research

    Article Title: SARS-CoV-2 Z-RNA activates the ZBP1-RIPK3 pathway to promote virus-induced inflammatory responses

    doi: 10.1038/s41422-022-00775-y

    Figure Lengend Snippet: a – e Ad5-hACE2 (2.5 × 10 8 PFU) transduced Ripk3 −/− , Mlkl −/− or wild-type C57BL/6 mice were infected with SARS-CoV-2 (1 × 10 5 TCID 50 ). Lung samples were harvested at 2 dpi. a The histopathological changes were evaluated by H&E staining. Inflammatory cell infiltration (green arrow) and alveolar septa expansion (red arrow) were indicated. Scale bars, 1000 μm in the original image and 50 μm in the enlarged image. b – j Immunostaining of CD45 ( b ), CD68 ( d ), CD3 ( f ) and MPO ( h ) representing the infiltration of leukocytes, macrophages, T cells and neutrophils respectively. b , f Scale bars, 50 μm and 10 μm in the enlarged image. d , h Scale bars, 50 μm. c , e , g , i Quantitative analysis of positive cells performed with ImageJ for b , d , f , h , respectively. Data shown are means ± SEM. Statistical significance was analyzed by Student’s t -test. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no significance. j Co-staining of CD8 (red) and CXCR3 (green) representing the recruitment of CD8 + CXCR3 + T cells in the lung section of SARS-CoV-2-infected wild-type and Ripk3 −/− mice. Scale bars, 10 μm.

    Article Snippet: Rabbit polyclonal anti-CD45 (20103-1-AP), anti-α-tubulin (11224-1-AP) and mouse IgG (B900620) were obtained from Proteintech (Chicago, IL, USA).

    Techniques: Infection, Staining, Immunostaining

    Disease‐related transcriptomic changes in EC‐KO. (A) IHC staining of Aβ, CD45, Iba1, and CD147 in 18‐month‐old APP/PS1 mouse brain slices and peer comparisons. Scale bar: 100 μm (B) The expression of DEGs whose human orthologs are related to neurodegenerative and cerebrovascular diseases in GWAS datasets, expression is represented as FPKM. Disease abbreviations: ALS, amyotrophic lateral sclerosis; WMH burden/SVD, white matter hyperintensity burden and cerebral small vessel disease; MSA, multiple systems atrophy; AD, Alzheimer's disease; PD, Parkinson's disease; FTD, frontotemporal dementia; HD, Huntington's disease. There were 21 DEGs related to AD ( p = 0.00266). (C) Visual PPI network of the AD‐related dataset. The edges between nodes represent different protein–protein associations, including experimental determination (purple), interactions from current databases (blue), and coexpression (black). (D) Expression changes of selected genes (App, Psen2, Epha1, Cd2ap, Bin1, Mef2c, Scarb2) by RT‐qPCR. Data are presented as the mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. (E) Human AD brain differential expression versus the control ( p < 0.05) served as the y‐axis, and DEGs (fold change ≥2 with FDR‐adjusted p < 0.05) from EC‐KO mice versus WT mice served as the x‐axis. The genes and their human orthologs were concordant in the first and third quadrants (light green), whereas they were discordant in the second and fourth quadrants (light red). Twenty‐one DEGs were specific in the groups compared at 8–12 weeks of age (red dot, EC‐KO versus WT), and 35 DEGs were specific in the groups compared at 6–7 months of age (green dot), whereas 11 DEGs were shared (blue dot) in both groups

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Endothelial genetic deletion of CD147 induces changes in the dual function of the blood‐brain barrier and is implicated in Alzheimer’s disease

    doi: 10.1111/cns.13659

    Figure Lengend Snippet: Disease‐related transcriptomic changes in EC‐KO. (A) IHC staining of Aβ, CD45, Iba1, and CD147 in 18‐month‐old APP/PS1 mouse brain slices and peer comparisons. Scale bar: 100 μm (B) The expression of DEGs whose human orthologs are related to neurodegenerative and cerebrovascular diseases in GWAS datasets, expression is represented as FPKM. Disease abbreviations: ALS, amyotrophic lateral sclerosis; WMH burden/SVD, white matter hyperintensity burden and cerebral small vessel disease; MSA, multiple systems atrophy; AD, Alzheimer's disease; PD, Parkinson's disease; FTD, frontotemporal dementia; HD, Huntington's disease. There were 21 DEGs related to AD ( p = 0.00266). (C) Visual PPI network of the AD‐related dataset. The edges between nodes represent different protein–protein associations, including experimental determination (purple), interactions from current databases (blue), and coexpression (black). (D) Expression changes of selected genes (App, Psen2, Epha1, Cd2ap, Bin1, Mef2c, Scarb2) by RT‐qPCR. Data are presented as the mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. (E) Human AD brain differential expression versus the control ( p < 0.05) served as the y‐axis, and DEGs (fold change ≥2 with FDR‐adjusted p < 0.05) from EC‐KO mice versus WT mice served as the x‐axis. The genes and their human orthologs were concordant in the first and third quadrants (light green), whereas they were discordant in the second and fourth quadrants (light red). Twenty‐one DEGs were specific in the groups compared at 8–12 weeks of age (red dot, EC‐KO versus WT), and 35 DEGs were specific in the groups compared at 6–7 months of age (green dot), whereas 11 DEGs were shared (blue dot) in both groups

    Article Snippet: Tissue slices were stained with primary antibodies, including rabbit CD147 monoclonal antibody (1:3000, Abcam), rabbit beta Amyloid (1‐42) polyclonal antibody (1:500, cat. GTX134510, GeneTex), rabbit CD45 polyclonal antibody (1:500, cat. 20103‐1‐AP, Proteintech), and rabbit Iba1 monoclonal antibody (1:2000), followed by HRP‐conjugated secondary antibodies and DAB substrate.

    Techniques: Immunohistochemistry, Expressing, Quantitative RT-PCR