cd34  (Sino Biological)


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    Name:
    CD34 cDNA ORF Clone in Cloning Vector Human
    Description:
    Full length Clone DNA of Human CD34 molecule transcript variant 2
    Catalog Number:
    HG10097-M
    Price:
    75.0
    Category:
    cDNA Clone
    Size:
    1Unit
    Molecule Name:
    CD34,AU040960,
    Buy from Supplier


    Structured Review

    Sino Biological cd34
    The proportion of <t>CD34</t> + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Full length Clone DNA of Human CD34 molecule transcript variant 2
    https://www.bioz.com/result/cd34/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd34 - by Bioz Stars, 2021-05
    93/100 stars

    Images

    1) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    2) Product Images from "Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus"

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2020.10922

    TIMP3, CD34 and substance P expression in rat NP tissue. Rats were percutaneously punctured with a 21G needle in coccygeal vertebra (puncture and TIMP3+puncture group). For TIMP3+puncture group, rats were injected with adenovirus vector (1×10 9 pfu/level) immediately after puncture. At day 28 after puncture, nucleus pulposus (NP) tissues were isolated for immunohistochemical staining. (A and B) Immunohistochemical staining was performed to measure TIMP3, CD34 and substance P expression in rat NP tissue. Overexpression of TIMP3 could reduce CD34 and substance P expression in NP tissue compared with the puncture group. Black arrow, positive staining. Data are presented as the mean ± SD. *P
    Figure Legend Snippet: TIMP3, CD34 and substance P expression in rat NP tissue. Rats were percutaneously punctured with a 21G needle in coccygeal vertebra (puncture and TIMP3+puncture group). For TIMP3+puncture group, rats were injected with adenovirus vector (1×10 9 pfu/level) immediately after puncture. At day 28 after puncture, nucleus pulposus (NP) tissues were isolated for immunohistochemical staining. (A and B) Immunohistochemical staining was performed to measure TIMP3, CD34 and substance P expression in rat NP tissue. Overexpression of TIMP3 could reduce CD34 and substance P expression in NP tissue compared with the puncture group. Black arrow, positive staining. Data are presented as the mean ± SD. *P

    Techniques Used: Expressing, Injection, Plasmid Preparation, Isolation, Immunohistochemistry, Staining, Over Expression

    3) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    4) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    5) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    6) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    7) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    8) Product Images from "Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus"

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2020.10922

    TIMP3, CD34 and substance P expression in rat NP tissue. Rats were percutaneously punctured with a 21G needle in coccygeal vertebra (puncture and TIMP3+puncture group). For TIMP3+puncture group, rats were injected with adenovirus vector (1×10 9 pfu/level) immediately after puncture. At day 28 after puncture, nucleus pulposus (NP) tissues were isolated for immunohistochemical staining. (A and B) Immunohistochemical staining was performed to measure TIMP3, CD34 and substance P expression in rat NP tissue. Overexpression of TIMP3 could reduce CD34 and substance P expression in NP tissue compared with the puncture group. Black arrow, positive staining. Data are presented as the mean ± SD. *P
    Figure Legend Snippet: TIMP3, CD34 and substance P expression in rat NP tissue. Rats were percutaneously punctured with a 21G needle in coccygeal vertebra (puncture and TIMP3+puncture group). For TIMP3+puncture group, rats were injected with adenovirus vector (1×10 9 pfu/level) immediately after puncture. At day 28 after puncture, nucleus pulposus (NP) tissues were isolated for immunohistochemical staining. (A and B) Immunohistochemical staining was performed to measure TIMP3, CD34 and substance P expression in rat NP tissue. Overexpression of TIMP3 could reduce CD34 and substance P expression in NP tissue compared with the puncture group. Black arrow, positive staining. Data are presented as the mean ± SD. *P

    Techniques Used: Expressing, Injection, Plasmid Preparation, Isolation, Immunohistochemistry, Staining, Over Expression

    9) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    10) Product Images from "Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus"

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2020.10922

    TIMP3, CD34 and substance P expression in rat NP tissue. Rats were percutaneously punctured with a 21G needle in coccygeal vertebra (puncture and TIMP3+puncture group). For TIMP3+puncture group, rats were injected with adenovirus vector (1×10 9 pfu/level) immediately after puncture. At day 28 after puncture, nucleus pulposus (NP) tissues were isolated for immunohistochemical staining. (A and B) Immunohistochemical staining was performed to measure TIMP3, CD34 and substance P expression in rat NP tissue. Overexpression of TIMP3 could reduce CD34 and substance P expression in NP tissue compared with the puncture group. Black arrow, positive staining. Data are presented as the mean ± SD. *P
    Figure Legend Snippet: TIMP3, CD34 and substance P expression in rat NP tissue. Rats were percutaneously punctured with a 21G needle in coccygeal vertebra (puncture and TIMP3+puncture group). For TIMP3+puncture group, rats were injected with adenovirus vector (1×10 9 pfu/level) immediately after puncture. At day 28 after puncture, nucleus pulposus (NP) tissues were isolated for immunohistochemical staining. (A and B) Immunohistochemical staining was performed to measure TIMP3, CD34 and substance P expression in rat NP tissue. Overexpression of TIMP3 could reduce CD34 and substance P expression in NP tissue compared with the puncture group. Black arrow, positive staining. Data are presented as the mean ± SD. *P

    Techniques Used: Expressing, Injection, Plasmid Preparation, Isolation, Immunohistochemistry, Staining, Over Expression

    11) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    12) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    Related Articles

    Expressing:

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: The similar KD values of the chimeric and humanized QBEND/10 indicated that the process of humanization did not alter the binding affinity of CD34. .. 3.6 Effects of various culture conditions on CD34 expression Previous data have shown that freshly isolated HUVECs are 90–95% CD34+ , but CD34 expression is rapidly lost when cells are cultured , . .. In this study, we kept HUVECs in culture (complete ECM) for a period of 7 days without being passaged, the proportion of CD34+ cells strongly increased (39.62%) at passage 8 ( A).

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus
    Article Snippet: .. TIMP3, CD34 and substance P expression levels were determined by immunohistochemical (IHC) staining. .. All staining procedures were performed following standard histochemical protocols.

    Isolation:

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: The similar KD values of the chimeric and humanized QBEND/10 indicated that the process of humanization did not alter the binding affinity of CD34. .. 3.6 Effects of various culture conditions on CD34 expression Previous data have shown that freshly isolated HUVECs are 90–95% CD34+ , but CD34 expression is rapidly lost when cells are cultured , . .. In this study, we kept HUVECs in culture (complete ECM) for a period of 7 days without being passaged, the proportion of CD34+ cells strongly increased (39.62%) at passage 8 ( A).

    Cell Culture:

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: The similar KD values of the chimeric and humanized QBEND/10 indicated that the process of humanization did not alter the binding affinity of CD34. .. 3.6 Effects of various culture conditions on CD34 expression Previous data have shown that freshly isolated HUVECs are 90–95% CD34+ , but CD34 expression is rapidly lost when cells are cultured , . .. In this study, we kept HUVECs in culture (complete ECM) for a period of 7 days without being passaged, the proportion of CD34+ cells strongly increased (39.62%) at passage 8 ( A).

    Staining:

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus
    Article Snippet: After injection of an adenovirus vector loading TIMP3, TIMP3 expression was significantly upregulated at day 28 ( ). .. The puncture group exhibited more positive CD34 and substance P staining, which indicated the neovascularization of IVDs after puncture. .. The positive staining rate of CD34 and substance P was significantly reduced in the TIMP3+puncture group compared with that in the control group ( ).

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus
    Article Snippet: .. TIMP3, CD34 and substance P expression levels were determined by immunohistochemical (IHC) staining. .. All staining procedures were performed following standard histochemical protocols.

    other:

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: Moreover, CD34+ HUVECs have been reported to show the antiangiogenic tip cell phenotype .

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: In this study, we kept HUVECs in culture (complete ECM) for a period of 7 days without being passaged, the proportion of CD34+ cells strongly increased (39.62%) at passage 8 ( A).

    Immunohistochemistry:

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus
    Article Snippet: .. TIMP3, CD34 and substance P expression levels were determined by immunohistochemical (IHC) staining. .. All staining procedures were performed following standard histochemical protocols.

    Binding Assay:

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: 3.5 Antibody binding analysis The binding affinities of two antibodies, chimeric and humanized QBEND/10, with recombinant CD34 were determined using a surface plasmon resonance biosensor-based assay, and the binding kinetics were determined. .. The KD of the binding of chimeric QBEND/10 with CD34 was 14.7 nM, whereas that of the humanized QBEND/10 with the CD34 protein was 7.34 nM ( ). ..

    Chromatin Immunoprecipitation:

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: 2.12 Surface plasmon resonance The binding kinetics of QBEND/10 antibodies to CD34 (Fc tag; Sino Biological Incorporation) were measured using the Biacore system (Biacore X, GE Healthcare) in the running buffer HBS-EP (10 mM HEPES, pH 7.4; 150 mM NaCl; 3 mM EDTA; 0.005% surfactant P20). .. In brief, CD34 was immobilized onto a CM5 sensor chip through amine coupling to a level of 1200 response units, and purified antibodies of different concentrations were injected at a flow rate of 30 μL/min. ..

    Purification:

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: 2.12 Surface plasmon resonance The binding kinetics of QBEND/10 antibodies to CD34 (Fc tag; Sino Biological Incorporation) were measured using the Biacore system (Biacore X, GE Healthcare) in the running buffer HBS-EP (10 mM HEPES, pH 7.4; 150 mM NaCl; 3 mM EDTA; 0.005% surfactant P20). .. In brief, CD34 was immobilized onto a CM5 sensor chip through amine coupling to a level of 1200 response units, and purified antibodies of different concentrations were injected at a flow rate of 30 μL/min. ..

    Injection:

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: 2.12 Surface plasmon resonance The binding kinetics of QBEND/10 antibodies to CD34 (Fc tag; Sino Biological Incorporation) were measured using the Biacore system (Biacore X, GE Healthcare) in the running buffer HBS-EP (10 mM HEPES, pH 7.4; 150 mM NaCl; 3 mM EDTA; 0.005% surfactant P20). .. In brief, CD34 was immobilized onto a CM5 sensor chip through amine coupling to a level of 1200 response units, and purified antibodies of different concentrations were injected at a flow rate of 30 μL/min. ..

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  • 88
    Sino Biological human cd34 protein
    The proportion of <t>CD34</t> + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Human Cd34 Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cd34 protein/product/Sino Biological
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human cd34 protein - by Bioz Stars, 2021-05
    88/100 stars
      Buy from Supplier

    93
    Sino Biological cd34
    The proportion of <t>CD34</t> + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Cd34, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd34/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd34 - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    N/A
    A DNA sequence encoding the extracellular domain of human CD34 precursor NP 001020280 1 Met 1 Thr 290 was expressed with a polyhistidine tag at the C terminus
      Buy from Supplier

    N/A
    The monoclonal antibody was purified by affinity chromatography Then it was conjugated with FITC under optimum conditions and the unreacted FITC was removed
      Buy from Supplier

    Image Search Results


    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Journal: Biochemistry and Biophysics Reports

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    doi: 10.1016/j.bbrep.2016.11.006

    Figure Lengend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Article Snippet: In brief, a Nunc™ MaxiSorp 96-well plate (Thermo Fisher Scientific) was coated with the human CD34 protein (Fc tag; Sino Biological Incorporation, Beijing, China) in a volume of 50 μL at a concentration of 5 µg/mL and incubated at 4 °C for 18 h. After blocking with the StartingBlock™ blocking buffer (Thermo Fisher Scientific) and being washing with PBS containing 0.01% Tween-20 (PBST) three times, the samples were added to the plates and incubated for 1 h at 37 °C.

    Techniques: Flow Cytometry, Incubation

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Journal: Biochemistry and Biophysics Reports

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    doi: 10.1016/j.bbrep.2016.11.006

    Figure Lengend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Article Snippet: In this study, we kept HUVECs in culture (complete ECM) for a period of 7 days without being passaged, the proportion of CD34+ cells strongly increased (39.62%) at passage 8 ( A).

    Techniques: Flow Cytometry, Incubation