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    Name:
    CD34 h PR
    Description:
    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of CD34 gene silencing results individual duplex components or plasmids are also available upon request Suitable control antibody CD34 Antibody B 6 sc 74499 is recommended as control antibody for monitoring of CD34 expression knockdown by Western blotting or immunofluorescence
    Catalog Number:
    SC-29249-PR
    Price:
    None
    Category:
    Gene Editing siRNA shRNA Gene Silencers Lymphocyte Signaling CD34 siRNA shRNA Plasmid and Lentiviral Particle Gene Silencers CD34 siRNA and shRNA Plasmids h
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    Structured Review

    Santa Cruz Biotechnology cd34
    Characterization of rat bone marrow-derived mesenchymal stromal cells (BMSCs). (a) The plastic-adherent cells demonstrated a homogeneous fibroblast-like and spindle-shaped morphology. Original magnification, ×100. (b) Adipogenic differentiation of BMSCs stained with oil red O. Original magnification, ×200. (c) Osteogenic differentiation of BM-MSCs stained with alizarin red. Original magnification, ×400. (d) FACS analysis demonstrated expression of markers attributed to BMSCs. The cells were devoid of hematopoietic cells as indicated by the lack of CD45 and <t>CD34.</t> The MSC-specific markers, CD29, CD44, CD73, CD105, and CD90 were strongly expressed on the cells.
    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of CD34 gene silencing results individual duplex components or plasmids are also available upon request Suitable control antibody CD34 Antibody B 6 sc 74499 is recommended as control antibody for monitoring of CD34 expression knockdown by Western blotting or immunofluorescence
    https://www.bioz.com/result/cd34/product/Santa Cruz Biotechnology
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd34 - by Bioz Stars, 2021-06
    94/100 stars

    Images

    1) Product Images from "Thioredoxin-1 Protects Bone Marrow-Derived Mesenchymal Stromal Cells from Hyperoxia-Induced Injury In Vitro"

    Article Title: Thioredoxin-1 Protects Bone Marrow-Derived Mesenchymal Stromal Cells from Hyperoxia-Induced Injury In Vitro

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2018/1023025

    Characterization of rat bone marrow-derived mesenchymal stromal cells (BMSCs). (a) The plastic-adherent cells demonstrated a homogeneous fibroblast-like and spindle-shaped morphology. Original magnification, ×100. (b) Adipogenic differentiation of BMSCs stained with oil red O. Original magnification, ×200. (c) Osteogenic differentiation of BM-MSCs stained with alizarin red. Original magnification, ×400. (d) FACS analysis demonstrated expression of markers attributed to BMSCs. The cells were devoid of hematopoietic cells as indicated by the lack of CD45 and CD34. The MSC-specific markers, CD29, CD44, CD73, CD105, and CD90 were strongly expressed on the cells.
    Figure Legend Snippet: Characterization of rat bone marrow-derived mesenchymal stromal cells (BMSCs). (a) The plastic-adherent cells demonstrated a homogeneous fibroblast-like and spindle-shaped morphology. Original magnification, ×100. (b) Adipogenic differentiation of BMSCs stained with oil red O. Original magnification, ×200. (c) Osteogenic differentiation of BM-MSCs stained with alizarin red. Original magnification, ×400. (d) FACS analysis demonstrated expression of markers attributed to BMSCs. The cells were devoid of hematopoietic cells as indicated by the lack of CD45 and CD34. The MSC-specific markers, CD29, CD44, CD73, CD105, and CD90 were strongly expressed on the cells.

    Techniques Used: Derivative Assay, Staining, FACS, Expressing

    2) Product Images from "Human adipose-derived stem cells inhibit bioactivity of keloid fibroblasts"

    Article Title: Human adipose-derived stem cells inhibit bioactivity of keloid fibroblasts

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-018-0786-4

    Adipose-derived stem cell-conditioned medium (ADSC-CM) reduces angiogenesis in keloid explant culture. After culturing with ( b , d ) or without ( a , c ) ADSC-CM for 8 days, the keloid explants were sectioned and subjected to immunohistochemical analysis using antibodies against CD31 ( a , b ) and CD34 ( c , d ). Brown coloration indicates positive staining of related markers. The numbers of CD31-positive endothelial cells and CD34-positive microvascular endothelial cells were counted in three randomly selected fields for each sample under a microscope. Scale bars = 100 μm. * P
    Figure Legend Snippet: Adipose-derived stem cell-conditioned medium (ADSC-CM) reduces angiogenesis in keloid explant culture. After culturing with ( b , d ) or without ( a , c ) ADSC-CM for 8 days, the keloid explants were sectioned and subjected to immunohistochemical analysis using antibodies against CD31 ( a , b ) and CD34 ( c , d ). Brown coloration indicates positive staining of related markers. The numbers of CD31-positive endothelial cells and CD34-positive microvascular endothelial cells were counted in three randomly selected fields for each sample under a microscope. Scale bars = 100 μm. * P

    Techniques Used: Derivative Assay, Immunohistochemistry, Staining, Microscopy

    Characterization of human adipose-derived stem cells (ADSCs). a Flow cytometric characterization of ADSCs. ADSCs strongly expressed CD29, CD44, CD90, and CD105, and did not express CD31, CD34, or CD45. b ADSCs exhibited a fibroblast-like morphology. c Cells were induced to differentiate into adipocytes (left panel) and osteoblasts (right panel); scale bars = 100 μm
    Figure Legend Snippet: Characterization of human adipose-derived stem cells (ADSCs). a Flow cytometric characterization of ADSCs. ADSCs strongly expressed CD29, CD44, CD90, and CD105, and did not express CD31, CD34, or CD45. b ADSCs exhibited a fibroblast-like morphology. c Cells were induced to differentiate into adipocytes (left panel) and osteoblasts (right panel); scale bars = 100 μm

    Techniques Used: Derivative Assay, Flow Cytometry

    3) Product Images from "Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors"

    Article Title: Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-9-362

    Effect of VEGF-C overexpression on angiogenesis of orthotopic tumors . H E-staining of representative PC-3/VEGF-C (A, orthotopic; C, subcutaneous) tumors and PC-3/mock (B, orthotopic; D, subcutaneous) tumors. PC-3/VEGF-C tumors showed angiogenic morphology with a rich network of capillaries compared with PC-3/mock tumors. There were significantly more blood capillaries (CD34 positive, arrows) in the PC-3/VEGF-C tumors (E, 220 ± 15 μm/mm 2 , n = 29) compared with PC-3/mock tumors (F and G, 37 ± 6 μm/mm 2 , n = 24), p
    Figure Legend Snippet: Effect of VEGF-C overexpression on angiogenesis of orthotopic tumors . H E-staining of representative PC-3/VEGF-C (A, orthotopic; C, subcutaneous) tumors and PC-3/mock (B, orthotopic; D, subcutaneous) tumors. PC-3/VEGF-C tumors showed angiogenic morphology with a rich network of capillaries compared with PC-3/mock tumors. There were significantly more blood capillaries (CD34 positive, arrows) in the PC-3/VEGF-C tumors (E, 220 ± 15 μm/mm 2 , n = 29) compared with PC-3/mock tumors (F and G, 37 ± 6 μm/mm 2 , n = 24), p

    Techniques Used: Over Expression, Staining

    4) Product Images from "Thioredoxin-1 Protects Bone Marrow-Derived Mesenchymal Stromal Cells from Hyperoxia-Induced Injury In Vitro"

    Article Title: Thioredoxin-1 Protects Bone Marrow-Derived Mesenchymal Stromal Cells from Hyperoxia-Induced Injury In Vitro

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2018/1023025

    Characterization of rat bone marrow-derived mesenchymal stromal cells (BMSCs). (a) The plastic-adherent cells demonstrated a homogeneous fibroblast-like and spindle-shaped morphology. Original magnification, ×100. (b) Adipogenic differentiation of BMSCs stained with oil red O. Original magnification, ×200. (c) Osteogenic differentiation of BM-MSCs stained with alizarin red. Original magnification, ×400. (d) FACS analysis demonstrated expression of markers attributed to BMSCs. The cells were devoid of hematopoietic cells as indicated by the lack of CD45 and CD34. The MSC-specific markers, CD29, CD44, CD73, CD105, and CD90 were strongly expressed on the cells.
    Figure Legend Snippet: Characterization of rat bone marrow-derived mesenchymal stromal cells (BMSCs). (a) The plastic-adherent cells demonstrated a homogeneous fibroblast-like and spindle-shaped morphology. Original magnification, ×100. (b) Adipogenic differentiation of BMSCs stained with oil red O. Original magnification, ×200. (c) Osteogenic differentiation of BM-MSCs stained with alizarin red. Original magnification, ×400. (d) FACS analysis demonstrated expression of markers attributed to BMSCs. The cells were devoid of hematopoietic cells as indicated by the lack of CD45 and CD34. The MSC-specific markers, CD29, CD44, CD73, CD105, and CD90 were strongly expressed on the cells.

    Techniques Used: Derivative Assay, Staining, FACS, Expressing

    5) Product Images from "Transmembrane Stem Cell Factor Protein Therapeutics Enhance Revascularization in Ischemia without Mast Cell Activation"

    Article Title: Transmembrane Stem Cell Factor Protein Therapeutics Enhance Revascularization in Ischemia without Mast Cell Activation

    Journal: bioRxiv

    doi: 10.1101/2020.04.06.028563

    EPCs are recruited to peripheral blood and ischemic site. (A and B) Representative immunostaining images of CD34 (red) and VE-cadherin (green) on WT mice calf and thigh muscle, respectively. Scale bar is 300 μm. (C) CD34 and VE-cadherin double positive areas were quantified in WT mice (n = 3 for thigh, n = 3-4for calf). **p
    Figure Legend Snippet: EPCs are recruited to peripheral blood and ischemic site. (A and B) Representative immunostaining images of CD34 (red) and VE-cadherin (green) on WT mice calf and thigh muscle, respectively. Scale bar is 300 μm. (C) CD34 and VE-cadherin double positive areas were quantified in WT mice (n = 3 for thigh, n = 3-4for calf). **p

    Techniques Used: Immunostaining, Mouse Assay

    Summary of the experimental findings in the studies. Mast cells use primarily a clathrin-mediated pathway to internalize SCF, leading to mast cell activation and anaphylaxis. In contrast, mast cells use predominantly clathrin and caveolin-mediated pathway to uptake tmSCF-based treatments and these treatments do not cause mast cell activation. Endothelial cells use both of clathrin- and caveolin-mediated pathway to uptake SCF, inducing angiogenesis in endothelial cells. Endothelial cells use both of clathrin- and caveolin-mediated pathway to internalize tmSCFPLs with medium uptake speed, triggering tube formation of endothelial cells and therapeutic angiogenesis. However, tmSCFNDs are internalized through clathrin/caveolin-mediated pathways with slow kinetics and do not induce an angiogenic response from mature endothelial cells. Endothelial progenitor cells (EPCs) use clathrin-mediated pathway to uptake SCF, triggering colony formation of EPCs and bone marrow cell mobilization. For tmSCF-based treatments, EPCs use a caveolin-mediated pathway for internalization leading to colony formation and angiogenesis. Treatment with tmSCFNDs further induced the mobilization of CD34 − CD133 + EPCs to the peripheral blood.
    Figure Legend Snippet: Summary of the experimental findings in the studies. Mast cells use primarily a clathrin-mediated pathway to internalize SCF, leading to mast cell activation and anaphylaxis. In contrast, mast cells use predominantly clathrin and caveolin-mediated pathway to uptake tmSCF-based treatments and these treatments do not cause mast cell activation. Endothelial cells use both of clathrin- and caveolin-mediated pathway to uptake SCF, inducing angiogenesis in endothelial cells. Endothelial cells use both of clathrin- and caveolin-mediated pathway to internalize tmSCFPLs with medium uptake speed, triggering tube formation of endothelial cells and therapeutic angiogenesis. However, tmSCFNDs are internalized through clathrin/caveolin-mediated pathways with slow kinetics and do not induce an angiogenic response from mature endothelial cells. Endothelial progenitor cells (EPCs) use clathrin-mediated pathway to uptake SCF, triggering colony formation of EPCs and bone marrow cell mobilization. For tmSCF-based treatments, EPCs use a caveolin-mediated pathway for internalization leading to colony formation and angiogenesis. Treatment with tmSCFNDs further induced the mobilization of CD34 − CD133 + EPCs to the peripheral blood.

    Techniques Used: Activation Assay

    EPC subpopulation was analyzed by flow cytometry. (A) Bone marrow cells treated with our treatments for 30min in vitro , then analyzed for CD34 − CD133 + CD146 + cells population. (B) CD34 − CD133 + CD146 + FLK1 + subpopulation in bone marrow was analyzed after four days of subcutaneous injection of our treatment. (C) CD34 + CD133 + CD146 + FLK1 + subpopulation in bone marrow was analyzed after four days of subcutaneous injection of our treatment.
    Figure Legend Snippet: EPC subpopulation was analyzed by flow cytometry. (A) Bone marrow cells treated with our treatments for 30min in vitro , then analyzed for CD34 − CD133 + CD146 + cells population. (B) CD34 − CD133 + CD146 + FLK1 + subpopulation in bone marrow was analyzed after four days of subcutaneous injection of our treatment. (C) CD34 + CD133 + CD146 + FLK1 + subpopulation in bone marrow was analyzed after four days of subcutaneous injection of our treatment.

    Techniques Used: Flow Cytometry, In Vitro, Injection

    6) Product Images from "Enhanced antitumor effects of BPD-MA-mediated photodynamic therapy combined with adriamycin on breast cancer in mice"

    Article Title: Enhanced antitumor effects of BPD-MA-mediated photodynamic therapy combined with adriamycin on breast cancer in mice

    Journal: Acta Pharmacologica Sinica

    doi: 10.1038/aps.2012.45

    Combined inhibition of tumor angiogenesis in vivo . (A) Immunohistochemical detection for CD34 expression in 4T1 breast carcinoma sc xenografted tumors. Representative pictures for different treatment groups are shown. The positive expression of CD34 was mainly represented as brownish yellow or brownish granules in tumor vascular endothelial cells. (B) The tumor MVD in different treatment groups. PDT plus ADM combination treatment synergistically inhibited in vivo angiogenesis of 4T1 breast carcinoma sc xenografted tumors ( c P
    Figure Legend Snippet: Combined inhibition of tumor angiogenesis in vivo . (A) Immunohistochemical detection for CD34 expression in 4T1 breast carcinoma sc xenografted tumors. Representative pictures for different treatment groups are shown. The positive expression of CD34 was mainly represented as brownish yellow or brownish granules in tumor vascular endothelial cells. (B) The tumor MVD in different treatment groups. PDT plus ADM combination treatment synergistically inhibited in vivo angiogenesis of 4T1 breast carcinoma sc xenografted tumors ( c P

    Techniques Used: Inhibition, In Vivo, Immunohistochemistry, Expressing

    7) Product Images from "Homing of Cultured Endothelial Progenitor Cells and Their Effect on Traumatic Brain Injury in Rat Model"

    Article Title: Homing of Cultured Endothelial Progenitor Cells and Their Effect on Traumatic Brain Injury in Rat Model

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-04153-2

    Effect of EPCs on angiogenesis. CD34 was used as a marker of microvascular endothelial cells to evaluate the effect of EPCs on angiogenesis. CD34+ cells expression in the EPCs transplanted group ( A , B ) and the control group ( C , D ). CD34+ endothelial–like vessel-lumen structure appeared in the brain tissue ( E ). The number of CD34+ cells in the traumatic zone increased after EPC transplantation as compared to the control group (P
    Figure Legend Snippet: Effect of EPCs on angiogenesis. CD34 was used as a marker of microvascular endothelial cells to evaluate the effect of EPCs on angiogenesis. CD34+ cells expression in the EPCs transplanted group ( A , B ) and the control group ( C , D ). CD34+ endothelial–like vessel-lumen structure appeared in the brain tissue ( E ). The number of CD34+ cells in the traumatic zone increased after EPC transplantation as compared to the control group (P

    Techniques Used: Marker, Expressing, Transplantation Assay

    Identification of EPCs. After seven days of culture, the cells were labeled with fluorescent antibodies against CD34 or CD133, and positive staining was seen in the cultured EPCs.
    Figure Legend Snippet: Identification of EPCs. After seven days of culture, the cells were labeled with fluorescent antibodies against CD34 or CD133, and positive staining was seen in the cultured EPCs.

    Techniques Used: Labeling, Staining, Cell Culture

    8) Product Images from "KERATOCAN EXPRESSION OF MURINE KERATOCYTES IS MAINTAINED ON AMNIOTIC MEMBRANE BY DOWNREGULATING TGF-? SIGNALING"

    Article Title: KERATOCAN EXPRESSION OF MURINE KERATOCYTES IS MAINTAINED ON AMNIOTIC MEMBRANE BY DOWNREGULATING TGF-? SIGNALING

    Journal:

    doi: 10.1074/jbc.M409567200

    CD34 and fibronectin expression. CD34 is not expressed (A), but fibronectin is expressed intracellularly and extracellularly (B) by P2 cells cultured on plastic. In contrast, CD34 is maintained (C) while fibronectin is not expressed (D) by P2 cells cultured
    Figure Legend Snippet: CD34 and fibronectin expression. CD34 is not expressed (A), but fibronectin is expressed intracellularly and extracellularly (B) by P2 cells cultured on plastic. In contrast, CD34 is maintained (C) while fibronectin is not expressed (D) by P2 cells cultured

    Techniques Used: Expressing, Cell Culture

    9) Product Images from "Diagnostic Value of Immunohistochemical Staining of GP73, GPC3, DCP, CD34, CD31, and Reticulin Staining in Hepatocellular Carcinoma"

    Article Title: Diagnostic Value of Immunohistochemical Staining of GP73, GPC3, DCP, CD34, CD31, and Reticulin Staining in Hepatocellular Carcinoma

    Journal: Journal of Histochemistry and Cytochemistry

    doi: 10.1369/0022155413492771

    CD34
    Figure Legend Snippet: CD34

    Techniques Used:

    Three CD34 immunostaining patterns. (A) Negative CD34 immunoreactive pattern in a case of hepatolithiasis: only the blood vessels and bile ducts in portal tracts were positive, whereas most sinusoidal spaces were negative. (B) Diffuse CD34 immunostaining
    Figure Legend Snippet: Three CD34 immunostaining patterns. (A) Negative CD34 immunoreactive pattern in a case of hepatolithiasis: only the blood vessels and bile ducts in portal tracts were positive, whereas most sinusoidal spaces were negative. (B) Diffuse CD34 immunostaining

    Techniques Used: Immunostaining

    Combination of GP73, GPC3, CD34, and Reticulin Staining in the Pathological Diagnosis of HCC
    Figure Legend Snippet: Combination of GP73, GPC3, CD34, and Reticulin Staining in the Pathological Diagnosis of HCC

    Techniques Used: Staining

    10) Product Images from "Clonally selected primitive endothelial cells promote occlusive pulmonary arteriopathy and severe pulmonary hypertension in rats exposed to chronic hypoxia"

    Article Title: Clonally selected primitive endothelial cells promote occlusive pulmonary arteriopathy and severe pulmonary hypertension in rats exposed to chronic hypoxia

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-58083-7

    Isolation and characterization of EC clones from CD117 + ECs. ( A ) Isolation and clonal enrichment flow diagram for EC clones from the lungs of EGFP + rats. ( B ) Representative differential interference contrast (DIC) image shows the typical endothelial cobblestone morphology of EC clones. ( C ) Representative DIC image of 2D matrigel assay. ( D ) Representative optical section (confocal microscopy) demonstrate binding of Griffonia simplicifolia lectin (red pseudo colour) in EC clones, indicating a microvascular phenotype (scale bar 25 μm). ( E ) 24 h 3D tube formation assay in fibrin shows formation of tube networks by EC clones (scale bar 200 μm). Cells were visualized by phalloidin staining of actin filaments (red pseudo colour). ( F ) confocal imaging of Matrigel plug at day 14 with EC clones stained for GFP (green pseudo colour) demonstrating GFP + blood vessels. The red autofluorescence demonstrates blood and indicates active perfusion of the GFP + vascular structures after 14 days. Scale bar: 25 μm. Counterstaining in ( D – F ) with DAPI. ( G , H ) Representative images demonstrate that EC clones form free-floating spheroids in low adhesion cell culture wells. When overlaid with a 50Vol%/50Vol% EGM2/Matrigel mixture, EC clone spheroids underwent angiogenic sprouting. The image ( G ) shows green fluorescence channel of EC clone spheroids (green pseudo colour), whereas image ( H ) shows DIC brightfield image of an EC clone spheroid after 7 days of sprouting. Scale bars: 50 μm. ( I ) Representative flow cytometry analysis of EC clones shows expression of endothelial markers (vWF, CD144, VEGFR2, CD105, CD34) and CD117, but not of typical hematopoietic and myeloid markers (CD45, CD11b/c and CD133). Note that vWF is an intracellular marker and required permeabilization of the cells during the staining process. ( J ) The fraction of clonally expandable wells increases from clonal generation 1 to 3. n = 3 (1st) and 6 (2nd, 3rd, 4th). ( K ) 4th generation EC clones proliferate faster than non-expanded CD117 + ECs (n = 3–4). * P
    Figure Legend Snippet: Isolation and characterization of EC clones from CD117 + ECs. ( A ) Isolation and clonal enrichment flow diagram for EC clones from the lungs of EGFP + rats. ( B ) Representative differential interference contrast (DIC) image shows the typical endothelial cobblestone morphology of EC clones. ( C ) Representative DIC image of 2D matrigel assay. ( D ) Representative optical section (confocal microscopy) demonstrate binding of Griffonia simplicifolia lectin (red pseudo colour) in EC clones, indicating a microvascular phenotype (scale bar 25 μm). ( E ) 24 h 3D tube formation assay in fibrin shows formation of tube networks by EC clones (scale bar 200 μm). Cells were visualized by phalloidin staining of actin filaments (red pseudo colour). ( F ) confocal imaging of Matrigel plug at day 14 with EC clones stained for GFP (green pseudo colour) demonstrating GFP + blood vessels. The red autofluorescence demonstrates blood and indicates active perfusion of the GFP + vascular structures after 14 days. Scale bar: 25 μm. Counterstaining in ( D – F ) with DAPI. ( G , H ) Representative images demonstrate that EC clones form free-floating spheroids in low adhesion cell culture wells. When overlaid with a 50Vol%/50Vol% EGM2/Matrigel mixture, EC clone spheroids underwent angiogenic sprouting. The image ( G ) shows green fluorescence channel of EC clone spheroids (green pseudo colour), whereas image ( H ) shows DIC brightfield image of an EC clone spheroid after 7 days of sprouting. Scale bars: 50 μm. ( I ) Representative flow cytometry analysis of EC clones shows expression of endothelial markers (vWF, CD144, VEGFR2, CD105, CD34) and CD117, but not of typical hematopoietic and myeloid markers (CD45, CD11b/c and CD133). Note that vWF is an intracellular marker and required permeabilization of the cells during the staining process. ( J ) The fraction of clonally expandable wells increases from clonal generation 1 to 3. n = 3 (1st) and 6 (2nd, 3rd, 4th). ( K ) 4th generation EC clones proliferate faster than non-expanded CD117 + ECs (n = 3–4). * P

    Techniques Used: Isolation, Clone Assay, Matrigel Assay, Confocal Microscopy, Binding Assay, Tube Formation Assay, Staining, Imaging, Cell Culture, Fluorescence, Flow Cytometry, Expressing, Marker

    11) Product Images from "Transplanted Endothelial Progenitor Cells Improve Ischemia Muscle Regeneration in Mice by Diffusion Tensor MR Imaging"

    Article Title: Transplanted Endothelial Progenitor Cells Improve Ischemia Muscle Regeneration in Mice by Diffusion Tensor MR Imaging

    Journal: Stem Cells International

    doi: 10.1155/2016/3641401

    Morphological changes and immunocytochemical analysis in mouse bone marrow-derived EPCs. (a) MNCs changed from globe-like shape to being thin and flat and then round and fusiform at day 7 (bar = 30 μ m). These cells were positive by indirect immunofluorescent staining for the markers of hematopoietic stem cells and progenitor cells, CD34, CD133, and CXCR4 (bar = 30 μ m). (b) The EPCs were able to take up Dil-labeled acetylated low-density lipoprotein (LDL) and bind the endothelial-specific lectin FITC-labeled lectin after 14 days in culture, which were colocalized in > 95% cell (bar = 30 μ m).
    Figure Legend Snippet: Morphological changes and immunocytochemical analysis in mouse bone marrow-derived EPCs. (a) MNCs changed from globe-like shape to being thin and flat and then round and fusiform at day 7 (bar = 30 μ m). These cells were positive by indirect immunofluorescent staining for the markers of hematopoietic stem cells and progenitor cells, CD34, CD133, and CXCR4 (bar = 30 μ m). (b) The EPCs were able to take up Dil-labeled acetylated low-density lipoprotein (LDL) and bind the endothelial-specific lectin FITC-labeled lectin after 14 days in culture, which were colocalized in > 95% cell (bar = 30 μ m).

    Techniques Used: Derivative Assay, Staining, Labeling

    In FACS analyses, EPCs cultured for 15 days were positive for CD34 ((b), 52.73%), VEGFR2 ((c), 61.63%), CD133 ((e), 14.28%), and the mature endothelial-specific marker CD31 ((f), 3.26%). Isotype controls were used in the FACS analyses ((a), (d)). A bar graph (g) showed that the positive rate of CD34, VEGFR2, CD133, and CD31. After being cultured for 30 days, the EPCs were positive for CD34 ((h), 15.65%).
    Figure Legend Snippet: In FACS analyses, EPCs cultured for 15 days were positive for CD34 ((b), 52.73%), VEGFR2 ((c), 61.63%), CD133 ((e), 14.28%), and the mature endothelial-specific marker CD31 ((f), 3.26%). Isotype controls were used in the FACS analyses ((a), (d)). A bar graph (g) showed that the positive rate of CD34, VEGFR2, CD133, and CD31. After being cultured for 30 days, the EPCs were positive for CD34 ((h), 15.65%).

    Techniques Used: FACS, Cell Culture, Marker

    12) Product Images from "Isolation and Characterization of Novel, Highly Proliferative Human CD34/CD73-Double-Positive Testis-Derived Stem Cells for Cell Therapy"

    Article Title: Isolation and Characterization of Novel, Highly Proliferative Human CD34/CD73-Double-Positive Testis-Derived Stem Cells for Cell Therapy

    Journal: Stem Cells and Development

    doi: 10.1089/scd.2012.0385

    (A) Specific marker expression in primary testis interstitial cells. Immunocytochemistry analysis reveals the expression of CD34 ( red, white arrowheads ) and GATA4 ( green, open arrowheads ) in the first row, 3β-HSD ( green, open arrowheads ) in the
    Figure Legend Snippet: (A) Specific marker expression in primary testis interstitial cells. Immunocytochemistry analysis reveals the expression of CD34 ( red, white arrowheads ) and GATA4 ( green, open arrowheads ) in the first row, 3β-HSD ( green, open arrowheads ) in the

    Techniques Used: Marker, Expressing, Immunocytochemistry

    Flow cytometric analyses of HTSCs. HTSCs were strongly positive for HLA-ABC, CD73, CD166, CD44, CD29, CD90, CD105, and CD34; weakly positive for CD14, CD133, and StroI; and negative for SSEA3, TRA-1-81, c-Kit, CD31, TRA-1-60, CD140, HLA-DR, CD45, and
    Figure Legend Snippet: Flow cytometric analyses of HTSCs. HTSCs were strongly positive for HLA-ABC, CD73, CD166, CD44, CD29, CD90, CD105, and CD34; weakly positive for CD14, CD133, and StroI; and negative for SSEA3, TRA-1-81, c-Kit, CD31, TRA-1-60, CD140, HLA-DR, CD45, and

    Techniques Used: Flow Cytometry

    13) Product Images from "Bone marrow derived “fibrocytes” contribute to tumor proliferation and fibrosis in gastric cancer"

    Article Title: Bone marrow derived “fibrocytes” contribute to tumor proliferation and fibrosis in gastric cancer

    Journal: Gastric Cancer

    doi: 10.1007/s10120-014-0380-0

    Morphology of fibrocytes isolated from cultures of peripheral blood mononuclear cells. a Cells cultured for 8 days in control medium ( left ). Cells cultured for 6 days in control medium and then for 2 days in control medium containing 5 ng/mL recombinant TGF-β1 ( right ). b Effects of TGF-β1 on fibrocyte differentiation in vitro. c Western blot analysis of the expression of CD34, collagen type I, and αSMA in these cultured cells. Each experiment was repeated three times, with the data expressed as means and standard deviations
    Figure Legend Snippet: Morphology of fibrocytes isolated from cultures of peripheral blood mononuclear cells. a Cells cultured for 8 days in control medium ( left ). Cells cultured for 6 days in control medium and then for 2 days in control medium containing 5 ng/mL recombinant TGF-β1 ( right ). b Effects of TGF-β1 on fibrocyte differentiation in vitro. c Western blot analysis of the expression of CD34, collagen type I, and αSMA in these cultured cells. Each experiment was repeated three times, with the data expressed as means and standard deviations

    Techniques Used: Isolation, Cell Culture, Recombinant, In Vitro, Western Blot, Expressing

    Co-culturing effects with MKN45 and fibrocytes. a Western blot analysis for E-cadherin and SDF-1 in MKN45 cells co-cultured with fibrocytes. b Western blot analysis for CD34, Collagen type I, αSMA, and CXCR4 in fibrocytes co-cultured with MKN45
    Figure Legend Snippet: Co-culturing effects with MKN45 and fibrocytes. a Western blot analysis for E-cadherin and SDF-1 in MKN45 cells co-cultured with fibrocytes. b Western blot analysis for CD34, Collagen type I, αSMA, and CXCR4 in fibrocytes co-cultured with MKN45

    Techniques Used: Western Blot, Cell Culture

    14) Product Images from "Multipotent mesenchymal progenitor cells are present in endarterectomized tissues from patients with chronic thromboembolic pulmonary hypertension"

    Article Title: Multipotent mesenchymal progenitor cells are present in endarterectomized tissues from patients with chronic thromboembolic pulmonary hypertension

    Journal: American Journal of Physiology - Cell Physiology

    doi: 10.1152/ajpcell.00416.2009

    CD105, CD166, CD44, CD73 positive, CD34 and CD45 negative cells are present in cells isolated from PTE tissues. A : representative histograms of fluorescent activated cells sorting for cell surface markers present or absent in mesenchymal stem cells.
    Figure Legend Snippet: CD105, CD166, CD44, CD73 positive, CD34 and CD45 negative cells are present in cells isolated from PTE tissues. A : representative histograms of fluorescent activated cells sorting for cell surface markers present or absent in mesenchymal stem cells.

    Techniques Used: Isolation

    15) Product Images from "Retinoblastoma Binding Protein 2 (RBP2) Promotes HIF-1α–VEGF-Induced Angiogenesis of Non-Small Cell Lung Cancer via the Akt Pathway"

    Article Title: Retinoblastoma Binding Protein 2 (RBP2) Promotes HIF-1α–VEGF-Induced Angiogenesis of Non-Small Cell Lung Cancer via the Akt Pathway

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0106032

    Immunohistochemical staining of the RBP2 protein and microvessels using the streptavidin–peroxidase method (magnification ×400). (A) High RBP2 protein expression in squamous cell cancer; (B) high RBP2 protein expression in adenocarcinoma; (C) negative RBP2 protein expression in NSCLC; (D) high HIF-1α expression in squamous cell cancer; (E) high HIF-1α protein expression in adenocarcinoma; (F) high VEGF expression in squamous cell cancer; (G) high VEGF in adenocarcinoma; (H) high CD34 expression in squamous cell cancer; (I) high CD34 expression in adenocarcinoma.
    Figure Legend Snippet: Immunohistochemical staining of the RBP2 protein and microvessels using the streptavidin–peroxidase method (magnification ×400). (A) High RBP2 protein expression in squamous cell cancer; (B) high RBP2 protein expression in adenocarcinoma; (C) negative RBP2 protein expression in NSCLC; (D) high HIF-1α expression in squamous cell cancer; (E) high HIF-1α protein expression in adenocarcinoma; (F) high VEGF expression in squamous cell cancer; (G) high VEGF in adenocarcinoma; (H) high CD34 expression in squamous cell cancer; (I) high CD34 expression in adenocarcinoma.

    Techniques Used: Immunohistochemistry, Staining, Expressing

    16) Product Images from "Reactive astrocytes promote adhesive interactions between brain endothelium and endothelial progenitor cells via HMGB1 and beta-2 integrin signaling"

    Article Title: Reactive astrocytes promote adhesive interactions between brain endothelium and endothelial progenitor cells via HMGB1 and beta-2 integrin signaling

    Journal: Stem cell research

    doi: 10.1016/j.scr.2013.12.008

    IL-1β-stimulated EPCs was enhanced the adhesive interaction to brain endothelial cells. (a) Early EPCs (5 days after seeding) were identified by representative markers including Flk1 and CD34. (b) Ac-LDL labeled EPCs adhered to rat brain endothelial
    Figure Legend Snippet: IL-1β-stimulated EPCs was enhanced the adhesive interaction to brain endothelial cells. (a) Early EPCs (5 days after seeding) were identified by representative markers including Flk1 and CD34. (b) Ac-LDL labeled EPCs adhered to rat brain endothelial

    Techniques Used: Labeling

    17) Product Images from "Transplantation of endothelial progenitor cells in treating rats with IgA nephropathy"

    Article Title: Transplantation of endothelial progenitor cells in treating rats with IgA nephropathy

    Journal: BMC Nephrology

    doi: 10.1186/1471-2369-15-110

    Identification of EPCs. The cells were collected and analyzed by flow cytometer for CD34, CD133 and FLK-1. The population of positive cells was indicated as percentage number in figures (A) . The cells were also fixed and dual stained with DiL-acLDL (red)/ FITC-UEA-1 (green). Merged images showed that most cells were dual-postive. Dual-postive cells were defined as EPCs (B) .
    Figure Legend Snippet: Identification of EPCs. The cells were collected and analyzed by flow cytometer for CD34, CD133 and FLK-1. The population of positive cells was indicated as percentage number in figures (A) . The cells were also fixed and dual stained with DiL-acLDL (red)/ FITC-UEA-1 (green). Merged images showed that most cells were dual-postive. Dual-postive cells were defined as EPCs (B) .

    Techniques Used: Flow Cytometry, Cytometry, Staining

    18) Product Images from "Bone-marrow mesenchymal stem cells reduce rat intestinal ischemia-reperfusion injury, ZO-1 downregulation and tight junction disruption via a TNF-?-regulated mechanism"

    Article Title: Bone-marrow mesenchymal stem cells reduce rat intestinal ischemia-reperfusion injury, ZO-1 downregulation and tight junction disruption via a TNF-?-regulated mechanism

    Journal: World Journal of Gastroenterology : WJG

    doi: 10.3748/wjg.v19.i23.3583

    Flow cytometric analysis of third-passage bone-marrow mesenchymal stem cells. A: The proportion of CD29-positive and CD34-negative cells was approximately 96%; B: The proportion of CD90-positive and CD450-negative was approximately 98%; C: The proportion
    Figure Legend Snippet: Flow cytometric analysis of third-passage bone-marrow mesenchymal stem cells. A: The proportion of CD29-positive and CD34-negative cells was approximately 96%; B: The proportion of CD90-positive and CD450-negative was approximately 98%; C: The proportion

    Techniques Used: Flow Cytometry

    19) Product Images from "Hydroxyapatite coating of cellulose sponges attracts bone-marrow-derived stem cells in rat subcutaneous tissue"

    Article Title: Hydroxyapatite coating of cellulose sponges attracts bone-marrow-derived stem cells in rat subcutaneous tissue

    Journal: Journal of the Royal Society Interface

    doi: 10.1098/rsif.2009.0020

    Stem cell markers in 7-day-old implants ((i) coated and (ii) uncoated). ( a ) Numerous c-kit-positive cells were observed in the sponge centre of HA-coated implants. ( b ) Anti-CD34-stained small rounded cells in the middle of the HA-coated sponge. ( c ) Stro-1,
    Figure Legend Snippet: Stem cell markers in 7-day-old implants ((i) coated and (ii) uncoated). ( a ) Numerous c-kit-positive cells were observed in the sponge centre of HA-coated implants. ( b ) Anti-CD34-stained small rounded cells in the middle of the HA-coated sponge. ( c ) Stro-1,

    Techniques Used: Staining

    20) Product Images from "Identification, Isolation and Expansion of Myoendothelial Cells Involved in Leech Muscle Regeneration"

    Article Title: Identification, Isolation and Expansion of Myoendothelial Cells Involved in Leech Muscle Regeneration

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0007652

    Effect of VEGF on cell differentiation in vitro . (A) Phase contrast image of a single colony that was picked with a micropipette and clonally expanded for a total of 3 days in vitro being exposed to VEGF from day 1 (B–H): Photomicrographs were obtained from clonally expanded colonies the were continually exposed to VEGF for a total of 20 days in vitro. (B) Expansion of a single colony in tissue medium. May Grunwald Giemsa staining (B1) and ultrastructural analysis (C) images show that the cultures consisted largely of rounded undifferentiated cells. (D) The Trypan blue assay showing that most of cells are viable (excluding the dye) after 20 days in VEGF supplemented medium (the blue background is due to vital dye precipitation). These cells are proliferating (E) as demonstrated by BrdU incorporation in their nuclei (arrowhead in E1). Double labelling staining showing the expression of desmin (red in F) and CD34 (green in F) or desmin (red in G) and FLK-1 (green in G). The cells exposed to VEGF in vitro for 20 days are MyHC − (red in H). Nuclei are stained with DAPI (blue in F, G, H). (I) Quantitative evaluation of elongated cell numbers. Column 1: cells cultured for 20 days in a VEGF free.medium. Column 2: cells cultured for 20 days in a VEGF supplemented medium.*p
    Figure Legend Snippet: Effect of VEGF on cell differentiation in vitro . (A) Phase contrast image of a single colony that was picked with a micropipette and clonally expanded for a total of 3 days in vitro being exposed to VEGF from day 1 (B–H): Photomicrographs were obtained from clonally expanded colonies the were continually exposed to VEGF for a total of 20 days in vitro. (B) Expansion of a single colony in tissue medium. May Grunwald Giemsa staining (B1) and ultrastructural analysis (C) images show that the cultures consisted largely of rounded undifferentiated cells. (D) The Trypan blue assay showing that most of cells are viable (excluding the dye) after 20 days in VEGF supplemented medium (the blue background is due to vital dye precipitation). These cells are proliferating (E) as demonstrated by BrdU incorporation in their nuclei (arrowhead in E1). Double labelling staining showing the expression of desmin (red in F) and CD34 (green in F) or desmin (red in G) and FLK-1 (green in G). The cells exposed to VEGF in vitro for 20 days are MyHC − (red in H). Nuclei are stained with DAPI (blue in F, G, H). (I) Quantitative evaluation of elongated cell numbers. Column 1: cells cultured for 20 days in a VEGF free.medium. Column 2: cells cultured for 20 days in a VEGF supplemented medium.*p

    Techniques Used: Cell Differentiation, In Vitro, Staining, BrdU Incorporation Assay, Expressing, Cell Culture

    Culture of cells recruited into the matrigel sponges by VEGF. After 1 week in vivo the MG was removed and the cells infiltrating the matrigel sponge were plated out at a low density and allowed to form colonies over 24 hrs. (A) Phase contrast image of Matrigel extracted after 1 week in vivo . It is infiltrated by numerous rounded cells that stained with Giemsa solution (A1). (B) Phase contrast image of small colonies after 24 hrs from seeding formed by rounded cells which stained with Giemsa (C) incorporate BrdU in their nuclei (arrowheads in D). (E–G) Double labelling experiments showing the expression in these cells of the myogenic marker MyoD (green in E–G) and the endothelial markers FLK-1 (red in E), VE-cadherin (red in F), and CD34 (red in G). (H) TEM micrograph of precursors cells showing an undifferentiated phenotype with large nucleus (n) and scarce cytoplasm. (I) Phase contrast image of a single colony that was picked with a micropipette and clonally expanded for a total of 3 days in vitro. (J–L) Double labelling experiments of clonal colonies showing the expression of MyoD (green in J–L) and the endothelial markers FLK-1 (red in J), VE-cadherin (red in K) or CD34 (red in L). (M) Clonal expansion of single colonies in phase contrast after 8 days in vitro. Rounded (arrowhead) and spindle-shape cells (arrow) that stained with Giemsa solution (N) are visible. (O) Positivity for BrdU incorporation is detectable only in the nuclei (arrowhead) of the rounded cells. Double immunofluorescence experiments detect the expression of CD34 (red in P) and MyoD (green in P) in the cultured cells. These cells are desmin + as well (red in Q). (R) TEM image showing thick filaments (arrowheads) in differentiating elongated cells. Phase contrast image (S) and Giemsa staining (T) of spindle-shape cells that dominate the clonal cultures after 20 days in vitro. The differentiating spindle shaped cells are BrdU − (U), CD34 + (green in V) and skeletal MyHC + (red in Z). (W) TEM photomicrograph showing a well differentiated muscle fibre with contractile material clearly organized into sarcomeres (arrowhead in W). Nuclei are counterstained with DAPI (blue in Q, V, Z). Bars in A–D: 100 µm; Bars in E–G: 20 µm Bars in I–L, V, Z 15 µm; Bars in M–Q, S–U: 10 µm; Bars in H, R, W: 400 nm.
    Figure Legend Snippet: Culture of cells recruited into the matrigel sponges by VEGF. After 1 week in vivo the MG was removed and the cells infiltrating the matrigel sponge were plated out at a low density and allowed to form colonies over 24 hrs. (A) Phase contrast image of Matrigel extracted after 1 week in vivo . It is infiltrated by numerous rounded cells that stained with Giemsa solution (A1). (B) Phase contrast image of small colonies after 24 hrs from seeding formed by rounded cells which stained with Giemsa (C) incorporate BrdU in their nuclei (arrowheads in D). (E–G) Double labelling experiments showing the expression in these cells of the myogenic marker MyoD (green in E–G) and the endothelial markers FLK-1 (red in E), VE-cadherin (red in F), and CD34 (red in G). (H) TEM micrograph of precursors cells showing an undifferentiated phenotype with large nucleus (n) and scarce cytoplasm. (I) Phase contrast image of a single colony that was picked with a micropipette and clonally expanded for a total of 3 days in vitro. (J–L) Double labelling experiments of clonal colonies showing the expression of MyoD (green in J–L) and the endothelial markers FLK-1 (red in J), VE-cadherin (red in K) or CD34 (red in L). (M) Clonal expansion of single colonies in phase contrast after 8 days in vitro. Rounded (arrowhead) and spindle-shape cells (arrow) that stained with Giemsa solution (N) are visible. (O) Positivity for BrdU incorporation is detectable only in the nuclei (arrowhead) of the rounded cells. Double immunofluorescence experiments detect the expression of CD34 (red in P) and MyoD (green in P) in the cultured cells. These cells are desmin + as well (red in Q). (R) TEM image showing thick filaments (arrowheads) in differentiating elongated cells. Phase contrast image (S) and Giemsa staining (T) of spindle-shape cells that dominate the clonal cultures after 20 days in vitro. The differentiating spindle shaped cells are BrdU − (U), CD34 + (green in V) and skeletal MyHC + (red in Z). (W) TEM photomicrograph showing a well differentiated muscle fibre with contractile material clearly organized into sarcomeres (arrowhead in W). Nuclei are counterstained with DAPI (blue in Q, V, Z). Bars in A–D: 100 µm; Bars in E–G: 20 µm Bars in I–L, V, Z 15 µm; Bars in M–Q, S–U: 10 µm; Bars in H, R, W: 400 nm.

    Techniques Used: In Vivo, Staining, Expressing, Marker, Transmission Electron Microscopy, In Vitro, BrdU Incorporation Assay, Immunofluorescence, Cell Culture

    Characterization of myoendothelial cells recruited into the matrigel sponges by VEGF. (A, B) Cryosections of MG, supplemented with VEGF and inoculated in leeches after an intramuscular Dil-Ac-LDL injection. After 1 week in vivo the matrigel implant contains rounded cells stained with Giemsa (A) and showing uptake of Dil-Ac-LDL (red in B). (C–I) Double labelling on serial cryosections of MG, supplemented with VEGF and inoculated in leeches. After 1 week in vivo , MG is infiltrated by cells expressing the MyoD muscle transcriptional factor (green in C–E and in the inserts) and the hematopoietic/endothelial precursor markers either CD34 (red in C and the insert), VE-cadherin (red in D and the insert), or Flk-1 (red in E and the insert). No staining is detected for the macrophage markers CD11c (red in F) CD68 (red in G) and CD14 (red in H). (I) Negative control where the primary antibodies were omitted and the secondary antibodies were applied together. After 15 days (J, K), MG is infiltrated by elongated Giemsa stained cells (J) that expressed desmin (green in K). After 1 month (L–N) groups of differentiated muscle fibres (L) are present in the MG sponge that are stained by the anti-skeletal MyHC A4.1025 antibody (green in M) and show the contractile material organized in sarcomeres with TEM (N). Nuclei are counterstained with DAPI (blue in B, K, M). (A1, J1, L1): cryosections of matrigel without VEGF injected as controls. Bars in A, A1, F-1: 100 µm; Bars in B–E: 50 µm; J, J1, K: 20 µm; Bars in L, L1, M: 10 µm; Bar in N: 2 µm.
    Figure Legend Snippet: Characterization of myoendothelial cells recruited into the matrigel sponges by VEGF. (A, B) Cryosections of MG, supplemented with VEGF and inoculated in leeches after an intramuscular Dil-Ac-LDL injection. After 1 week in vivo the matrigel implant contains rounded cells stained with Giemsa (A) and showing uptake of Dil-Ac-LDL (red in B). (C–I) Double labelling on serial cryosections of MG, supplemented with VEGF and inoculated in leeches. After 1 week in vivo , MG is infiltrated by cells expressing the MyoD muscle transcriptional factor (green in C–E and in the inserts) and the hematopoietic/endothelial precursor markers either CD34 (red in C and the insert), VE-cadherin (red in D and the insert), or Flk-1 (red in E and the insert). No staining is detected for the macrophage markers CD11c (red in F) CD68 (red in G) and CD14 (red in H). (I) Negative control where the primary antibodies were omitted and the secondary antibodies were applied together. After 15 days (J, K), MG is infiltrated by elongated Giemsa stained cells (J) that expressed desmin (green in K). After 1 month (L–N) groups of differentiated muscle fibres (L) are present in the MG sponge that are stained by the anti-skeletal MyHC A4.1025 antibody (green in M) and show the contractile material organized in sarcomeres with TEM (N). Nuclei are counterstained with DAPI (blue in B, K, M). (A1, J1, L1): cryosections of matrigel without VEGF injected as controls. Bars in A, A1, F-1: 100 µm; Bars in B–E: 50 µm; J, J1, K: 20 µm; Bars in L, L1, M: 10 µm; Bar in N: 2 µm.

    Techniques Used: Injection, In Vivo, Staining, Expressing, Negative Control, Transmission Electron Microscopy

    Double immunolocalization of endothelial and myogenic markers in the interstitial spaces of leech skeletal muscle. (A–E) Cryosections of unlesioned leech. (F–J) Serial cryosection of injured leeches. Small rounded cells (arrows) dispersed among muscle (m) in control animals (A–E) and close to the regenerating area (arrowheads) in wounded leeches (F–J) express the MyoD muscle transcriptional factor (green) and the hematopoietic and endothelial markers CD34 (in red A, F), VE-cadherin (in red B, G), Flk-1 (in red C, H), CD31 (in red D, I). CD68 + cells (in red E, J) are MyoD − (in green E, J) and in injured animals (J) are mainly located in the regenerating area (arrowhead). The muscle cells (m) do not express the endothelial or the macrophage markers (A–E). (K) The percentage of cells expressing myogenic, endothelial and macrophage markers. (L) Expression of CD markers in injured leeches. The antibodies detected a band of 120 kDa for CD31 (lane 2), 140 kDa for CD34 (lane 3). The molecular weights are in accordance with those of the corresponding vertebrate molecules. Lanes 1 molecular weight standards. Lanes 4: negative controls. Bars in A–J: 100 µm.
    Figure Legend Snippet: Double immunolocalization of endothelial and myogenic markers in the interstitial spaces of leech skeletal muscle. (A–E) Cryosections of unlesioned leech. (F–J) Serial cryosection of injured leeches. Small rounded cells (arrows) dispersed among muscle (m) in control animals (A–E) and close to the regenerating area (arrowheads) in wounded leeches (F–J) express the MyoD muscle transcriptional factor (green) and the hematopoietic and endothelial markers CD34 (in red A, F), VE-cadherin (in red B, G), Flk-1 (in red C, H), CD31 (in red D, I). CD68 + cells (in red E, J) are MyoD − (in green E, J) and in injured animals (J) are mainly located in the regenerating area (arrowhead). The muscle cells (m) do not express the endothelial or the macrophage markers (A–E). (K) The percentage of cells expressing myogenic, endothelial and macrophage markers. (L) Expression of CD markers in injured leeches. The antibodies detected a band of 120 kDa for CD31 (lane 2), 140 kDa for CD34 (lane 3). The molecular weights are in accordance with those of the corresponding vertebrate molecules. Lanes 1 molecular weight standards. Lanes 4: negative controls. Bars in A–J: 100 µm.

    Techniques Used: Expressing, Molecular Weight

    21) Product Images from "Infiltrated pre-adipocytes increase prostate cancer metastasis via modulation of the miR-301a/androgen receptor (AR)/TGF-β1/Smad/MMP9 signals"

    Article Title: Infiltrated pre-adipocytes increase prostate cancer metastasis via modulation of the miR-301a/androgen receptor (AR)/TGF-β1/Smad/MMP9 signals

    Journal: Oncotarget

    doi:

    Prostate cancer recruits more pre-adipocytes than normal prostate A. Pref-1 and CD34 staining to show pre-adipocyte distribution in human PCa and adjacent normal tissues by immunofluorescence co-staining. B. Quantification of pre-adipocyte numbers in PCa and normal prostate tissues. C. Cartoon illustration of the pre-adipocyte recruitment to PCa and normal prostate epithelial cells experimental procedure. Pre-adipocyte cells (5 × 10 4 ) were placed in the upper chambers and the PCa cells were cultured in the bottom chambers to assay the migration of pre-adipocyte cells. After 24 hours, the membranes were fixed and stained to visualize the migrated pre-adipocyte cells on the bottom of the membrane. D. The representative figures and quantitative data of pre-adipocyte recruitment migration by normal prostate cell, RWPE-1, and PCa C4-2 and CWR22Rv1 (22Rv1) cells. *** p
    Figure Legend Snippet: Prostate cancer recruits more pre-adipocytes than normal prostate A. Pref-1 and CD34 staining to show pre-adipocyte distribution in human PCa and adjacent normal tissues by immunofluorescence co-staining. B. Quantification of pre-adipocyte numbers in PCa and normal prostate tissues. C. Cartoon illustration of the pre-adipocyte recruitment to PCa and normal prostate epithelial cells experimental procedure. Pre-adipocyte cells (5 × 10 4 ) were placed in the upper chambers and the PCa cells were cultured in the bottom chambers to assay the migration of pre-adipocyte cells. After 24 hours, the membranes were fixed and stained to visualize the migrated pre-adipocyte cells on the bottom of the membrane. D. The representative figures and quantitative data of pre-adipocyte recruitment migration by normal prostate cell, RWPE-1, and PCa C4-2 and CWR22Rv1 (22Rv1) cells. *** p

    Techniques Used: Staining, Immunofluorescence, Cell Culture, Migration

    22) Product Images from "Hematopoietic stem cells and progenitors of chronic myeloid leukemia express leukemia-associated antigens: implications for the graft-versus-leukemia effect and peptide vaccine-based immunotherapy"

    Article Title: Hematopoietic stem cells and progenitors of chronic myeloid leukemia express leukemia-associated antigens: implications for the graft-versus-leukemia effect and peptide vaccine-based immunotherapy

    Journal:

    doi: 10.1038/leu.2008.161

    Expression of genes encoding leukemia-associated antigens in CD34+ subpopulations of healthy individuals and CML patients
    Figure Legend Snippet: Expression of genes encoding leukemia-associated antigens in CD34+ subpopulations of healthy individuals and CML patients

    Techniques Used: Expressing

    Expression of genes encoding minor histocompatibility antigens in CD34+ subpopulations of CML patients
    Figure Legend Snippet: Expression of genes encoding minor histocompatibility antigens in CD34+ subpopulations of CML patients

    Techniques Used: Expressing

    Spectrum of graft-versus-leukemia target antigen expression according to disease phase and CD34+ progenitor maturation in CML
    Figure Legend Snippet: Spectrum of graft-versus-leukemia target antigen expression according to disease phase and CD34+ progenitor maturation in CML

    Techniques Used: Expressing

    23) Product Images from "Colorectal cancer-promoting activity of the senescent peritoneal mesothelium"

    Article Title: Colorectal cancer-promoting activity of the senescent peritoneal mesothelium

    Journal: Oncotarget

    doi:

    Microscopic evaluation of tumor vascularization of xenografts excised from the mouse peritoneal cavity after i.p. injection of SW480 and PSN-1 cells together with young or senescent (sen) HPMCs The development of microvessels was analyzed according to immunohistochemical reactions against CD31 A, B. and CD34 C, D. i.e. markers of endothelial cells. Panels A and C. show the representative results of CD31 and CD34 staining (the brown color indicates a positive reaction; magnification x100). Panels B and D. show the results of the quantification of the brown-stained area reflecting the presence of CD31- B. and CD34-positive cells D. The results are expressed as a percentage (%), and the whole area of a specimen is considered to be 100%. The asterisks indicate a significant difference ( P
    Figure Legend Snippet: Microscopic evaluation of tumor vascularization of xenografts excised from the mouse peritoneal cavity after i.p. injection of SW480 and PSN-1 cells together with young or senescent (sen) HPMCs The development of microvessels was analyzed according to immunohistochemical reactions against CD31 A, B. and CD34 C, D. i.e. markers of endothelial cells. Panels A and C. show the representative results of CD31 and CD34 staining (the brown color indicates a positive reaction; magnification x100). Panels B and D. show the results of the quantification of the brown-stained area reflecting the presence of CD31- B. and CD34-positive cells D. The results are expressed as a percentage (%), and the whole area of a specimen is considered to be 100%. The asterisks indicate a significant difference ( P

    Techniques Used: Injection, Immunohistochemistry, Staining

    24) Product Images from "Expansion of Endothelial Progenitor Cells in High Density Dot Culture of Rat Bone Marrow Cells"

    Article Title: Expansion of Endothelial Progenitor Cells in High Density Dot Culture of Rat Bone Marrow Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0107127

    Endothelial precursors are enriched in the CD45 + cell population. A, Flow cytometric analysis showed that the majority of cells in regular density (RD) culture became larger after their expansion, whereas the cells in high density (HD) culture maintained the small size of their parental cells (P0). B, Cells from high density culture were analyzed further by gating based on different cell sizes. The small cells showed higher expression levels of EPC related markers than those of the large cells. C, In high density culture, the majority of cells positive for KDR, CD34 and DiI were also positive for CD45. D, In vitro angiogenesis assay of CD45 + and CD45 − cells sorted from high density culture. E, CD45 + , but not CD45 − , cells from high density culture differentiated into endothelial cells that were able to uptake DiI-ac-LDL and express vWF and eNOs. Scale bars, 100 µm. F, Percentages of endothelial cell marker-positive cells in CD45 + and CD45 − populations after 14 days of induction (n = 3). *p
    Figure Legend Snippet: Endothelial precursors are enriched in the CD45 + cell population. A, Flow cytometric analysis showed that the majority of cells in regular density (RD) culture became larger after their expansion, whereas the cells in high density (HD) culture maintained the small size of their parental cells (P0). B, Cells from high density culture were analyzed further by gating based on different cell sizes. The small cells showed higher expression levels of EPC related markers than those of the large cells. C, In high density culture, the majority of cells positive for KDR, CD34 and DiI were also positive for CD45. D, In vitro angiogenesis assay of CD45 + and CD45 − cells sorted from high density culture. E, CD45 + , but not CD45 − , cells from high density culture differentiated into endothelial cells that were able to uptake DiI-ac-LDL and express vWF and eNOs. Scale bars, 100 µm. F, Percentages of endothelial cell marker-positive cells in CD45 + and CD45 − populations after 14 days of induction (n = 3). *p

    Techniques Used: Flow Cytometry, Expressing, In Vitro, Angiogenesis Assay, Marker

    25) Product Images from "Expansion of Endothelial Progenitor Cells in High Density Dot Culture of Rat Bone Marrow Cells"

    Article Title: Expansion of Endothelial Progenitor Cells in High Density Dot Culture of Rat Bone Marrow Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0107127

    Endothelial precursors are enriched in the CD45 + cell population. A, Flow cytometric analysis showed that the majority of cells in regular density (RD) culture became larger after their expansion, whereas the cells in high density (HD) culture maintained the small size of their parental cells (P0). B, Cells from high density culture were analyzed further by gating based on different cell sizes. The small cells showed higher expression levels of EPC related markers than those of the large cells. C, In high density culture, the majority of cells positive for KDR, CD34 and DiI were also positive for CD45. D, In vitro angiogenesis assay of CD45 + and CD45 − cells sorted from high density culture. E, CD45 + , but not CD45 − , cells from high density culture differentiated into endothelial cells that were able to uptake DiI-ac-LDL and express vWF and eNOs. Scale bars, 100 µm. F, Percentages of endothelial cell marker-positive cells in CD45 + and CD45 − populations after 14 days of induction (n = 3). *p
    Figure Legend Snippet: Endothelial precursors are enriched in the CD45 + cell population. A, Flow cytometric analysis showed that the majority of cells in regular density (RD) culture became larger after their expansion, whereas the cells in high density (HD) culture maintained the small size of their parental cells (P0). B, Cells from high density culture were analyzed further by gating based on different cell sizes. The small cells showed higher expression levels of EPC related markers than those of the large cells. C, In high density culture, the majority of cells positive for KDR, CD34 and DiI were also positive for CD45. D, In vitro angiogenesis assay of CD45 + and CD45 − cells sorted from high density culture. E, CD45 + , but not CD45 − , cells from high density culture differentiated into endothelial cells that were able to uptake DiI-ac-LDL and express vWF and eNOs. Scale bars, 100 µm. F, Percentages of endothelial cell marker-positive cells in CD45 + and CD45 − populations after 14 days of induction (n = 3). *p

    Techniques Used: Flow Cytometry, Expressing, In Vitro, Angiogenesis Assay, Marker

    26) Product Images from "Telocytes in the human kidney cortex"

    Article Title: Telocytes in the human kidney cortex

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/j.1582-4934.2012.01582.x

    In situ IHC of CD117, CD34, vimentin, CD31, D2–40 and Tryptase on serial slides. One cell with positive expression of CD117 can be seen in the kidney interstitium (A; magenta arrow). It has three long and thin Tps. Positive expressions of CD34 and vimentin can be observed in the same area of serial slides (B) and (C). Staining results of CD31, D2–40 and tryptase are presented (D)–(F) on serial slides. Their negative expressions in same area help us exclude mast cell staining of CD117 and endothelial cell staining of CD34. Magnification: 400×.
    Figure Legend Snippet: In situ IHC of CD117, CD34, vimentin, CD31, D2–40 and Tryptase on serial slides. One cell with positive expression of CD117 can be seen in the kidney interstitium (A; magenta arrow). It has three long and thin Tps. Positive expressions of CD34 and vimentin can be observed in the same area of serial slides (B) and (C). Staining results of CD31, D2–40 and tryptase are presented (D)–(F) on serial slides. Their negative expressions in same area help us exclude mast cell staining of CD117 and endothelial cell staining of CD34. Magnification: 400×.

    Techniques Used: In Situ, Immunohistochemistry, Expressing, Staining

    Immunofluorescence labelling for CD117, CD34 and vimentin of cultured cells. (A) FITC labelling for CD117 (green). (B) FITC labelling for CD34 (yellow). (C) TRITC labelling for vimentin (red). DAPI staining for nuclei (blue) in (A), (B), and (C). FITC: fluorescein isothiocyanate; TRITC: tetraethyl rhodamine isothiocyanate; DAPI: 4′, 6-diamidino-2-phenylindole.
    Figure Legend Snippet: Immunofluorescence labelling for CD117, CD34 and vimentin of cultured cells. (A) FITC labelling for CD117 (green). (B) FITC labelling for CD34 (yellow). (C) TRITC labelling for vimentin (red). DAPI staining for nuclei (blue) in (A), (B), and (C). FITC: fluorescein isothiocyanate; TRITC: tetraethyl rhodamine isothiocyanate; DAPI: 4′, 6-diamidino-2-phenylindole.

    Techniques Used: Immunofluorescence, Cell Culture, Staining

    27) Product Images from "The carboxyl terminal trimer of procollagen I induces pro-metastatic changes and vascularization in breast cancer cells xenografts"

    Article Title: The carboxyl terminal trimer of procollagen I induces pro-metastatic changes and vascularization in breast cancer cells xenografts

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-9-59

    Vessel formation in subcutaneous tumors in xenografts of MDA MB231 in presence or absence of PICP . Immunohistochemistry and immunofluorescence identify CD31- and CD34-expressing endothelial cells. Exemplary micrographs of tumors are shown out of 3 to 9 tumors examined for each time point. Left: Sections from criopreserved tumors dissected after 14, 21 and 28 days are processed for immunohistochemistry with CD31 antibody, followed by a secondary antibody conjugated either with TRITC (A, B and G, H) or biotin (C-F). Bar is 50 μm for A-D and G-H, and 25 μm for E-F. Sections from paraffin embedded tumors dissected after 24 and 38 days are decorated with antibody to CD34 and revealed by Immunoperoxidase.100× Bar is 50 μm.
    Figure Legend Snippet: Vessel formation in subcutaneous tumors in xenografts of MDA MB231 in presence or absence of PICP . Immunohistochemistry and immunofluorescence identify CD31- and CD34-expressing endothelial cells. Exemplary micrographs of tumors are shown out of 3 to 9 tumors examined for each time point. Left: Sections from criopreserved tumors dissected after 14, 21 and 28 days are processed for immunohistochemistry with CD31 antibody, followed by a secondary antibody conjugated either with TRITC (A, B and G, H) or biotin (C-F). Bar is 50 μm for A-D and G-H, and 25 μm for E-F. Sections from paraffin embedded tumors dissected after 24 and 38 days are decorated with antibody to CD34 and revealed by Immunoperoxidase.100× Bar is 50 μm.

    Techniques Used: Multiple Displacement Amplification, Immunohistochemistry, Immunofluorescence, Expressing

    28) Product Images from "Adipose-Derived Mesenchymal Stromal Cells Improve the Healing of Colonic Anastomoses Following High Dose of Irradiation Through Anti-Inflammatory and Angiogenic Processes"

    Article Title: Adipose-Derived Mesenchymal Stromal Cells Improve the Healing of Colonic Anastomoses Following High Dose of Irradiation Through Anti-Inflammatory and Angiogenic Processes

    Journal: Cell Transplantation

    doi: 10.1177/0963689717721515

    Characterization of MSCs before injection. (a) Morphology of the Ad-MSCs in culture before injection (b) Representative picture of colony-forming unit-fibroblast (CFU-F) assay stained with crystal violet. The CFU-F assay has been used to enumerate the number of Ad-MSCs within the initial cell preparation. (c) Flow cytometry analysis of MSCs before injection. MSCs expressed high levels of CD90, CD73 and CD29 specific markers (upper panel). MSCs did not express hematopoietic marker as CD34 and CD45 (lower panel).
    Figure Legend Snippet: Characterization of MSCs before injection. (a) Morphology of the Ad-MSCs in culture before injection (b) Representative picture of colony-forming unit-fibroblast (CFU-F) assay stained with crystal violet. The CFU-F assay has been used to enumerate the number of Ad-MSCs within the initial cell preparation. (c) Flow cytometry analysis of MSCs before injection. MSCs expressed high levels of CD90, CD73 and CD29 specific markers (upper panel). MSCs did not express hematopoietic marker as CD34 and CD45 (lower panel).

    Techniques Used: Injection, Staining, Flow Cytometry, Cytometry, Marker

    29) Product Images from "Transplanted Endothelial Progenitor Cells Improve Ischemia Muscle Regeneration in Mice by Diffusion Tensor MR Imaging"

    Article Title: Transplanted Endothelial Progenitor Cells Improve Ischemia Muscle Regeneration in Mice by Diffusion Tensor MR Imaging

    Journal: Stem Cells International

    doi: 10.1155/2016/3641401

    Morphological changes and immunocytochemical analysis in mouse bone marrow-derived EPCs. (a) MNCs changed from globe-like shape to being thin and flat and then round and fusiform at day 7 (bar = 30 μ m). These cells were positive by indirect immunofluorescent staining for the markers of hematopoietic stem cells and progenitor cells, CD34, CD133, and CXCR4 (bar = 30 μ m). (b) The EPCs were able to take up Dil-labeled acetylated low-density lipoprotein (LDL) and bind the endothelial-specific lectin FITC-labeled lectin after 14 days in culture, which were colocalized in > 95% cell (bar = 30 μ m).
    Figure Legend Snippet: Morphological changes and immunocytochemical analysis in mouse bone marrow-derived EPCs. (a) MNCs changed from globe-like shape to being thin and flat and then round and fusiform at day 7 (bar = 30 μ m). These cells were positive by indirect immunofluorescent staining for the markers of hematopoietic stem cells and progenitor cells, CD34, CD133, and CXCR4 (bar = 30 μ m). (b) The EPCs were able to take up Dil-labeled acetylated low-density lipoprotein (LDL) and bind the endothelial-specific lectin FITC-labeled lectin after 14 days in culture, which were colocalized in > 95% cell (bar = 30 μ m).

    Techniques Used: Derivative Assay, Staining, Labeling

    In FACS analyses, EPCs cultured for 15 days were positive for CD34 ((b), 52.73%), VEGFR2 ((c), 61.63%), CD133 ((e), 14.28%), and the mature endothelial-specific marker CD31 ((f), 3.26%). Isotype controls were used in the FACS analyses ((a), (d)). A bar graph (g) showed that the positive rate of CD34, VEGFR2, CD133, and CD31. After being cultured for 30 days, the EPCs were positive for CD34 ((h), 15.65%).
    Figure Legend Snippet: In FACS analyses, EPCs cultured for 15 days were positive for CD34 ((b), 52.73%), VEGFR2 ((c), 61.63%), CD133 ((e), 14.28%), and the mature endothelial-specific marker CD31 ((f), 3.26%). Isotype controls were used in the FACS analyses ((a), (d)). A bar graph (g) showed that the positive rate of CD34, VEGFR2, CD133, and CD31. After being cultured for 30 days, the EPCs were positive for CD34 ((h), 15.65%).

    Techniques Used: FACS, Cell Culture, Marker

    30) Product Images from "Differential Effects of Isoxazole-9 on Neural Stem/Progenitor Cells, Oligodendrocyte Precursor Cells, and Endothelial Progenitor Cells"

    Article Title: Differential Effects of Isoxazole-9 on Neural Stem/Progenitor Cells, Oligodendrocyte Precursor Cells, and Endothelial Progenitor Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0138724

    Early EPC cultures. Immunohistochmistry showed that our early EPC cultures were positive for CD31, CD34, CD133, Flk-1/VEGFR2, vWF, and UFA-1. Nuclei were stained by DAPI shown in blue.
    Figure Legend Snippet: Early EPC cultures. Immunohistochmistry showed that our early EPC cultures were positive for CD31, CD34, CD133, Flk-1/VEGFR2, vWF, and UFA-1. Nuclei were stained by DAPI shown in blue.

    Techniques Used: Staining

    Outgrowh EPC cultures. Immunohistochmistry showed that our outgrowth EPC cultures were positive for CD31, CD34, Flk-1/VEGFR2, vWF, and UFA-1, but negative for CD133. Nuclei were stained by DAPI shown in blue.
    Figure Legend Snippet: Outgrowh EPC cultures. Immunohistochmistry showed that our outgrowth EPC cultures were positive for CD31, CD34, Flk-1/VEGFR2, vWF, and UFA-1, but negative for CD133. Nuclei were stained by DAPI shown in blue.

    Techniques Used: Staining

    31) Product Images from "Reconstruction of the corneal epithelium with induced marrow mesenchymal stem cells in rats"

    Article Title: Reconstruction of the corneal epithelium with induced marrow mesenchymal stem cells in rats

    Journal: Molecular Vision

    doi:

    Subcultured mesenchymal stem cells (MSCs) in vitro. A : Phase contrast micrographs of mesenchymal stem cells cultured on day seven (40×). B : Immunofluorescence staining of CD29 was positive. C : Flow cytometric analysis showed that the positive rates of CD29, CD34, CD44, CD45, CD71, CD90, and CD133 were 81.56%, 0.10%, 88.77%, 0.17%, 10.02%, 98.43%, and 1.56%, respectively. D : Scanning electron microscopy (SEM) of MSCs showed a long spindle-shaped appearance with rich processes (1000×).
    Figure Legend Snippet: Subcultured mesenchymal stem cells (MSCs) in vitro. A : Phase contrast micrographs of mesenchymal stem cells cultured on day seven (40×). B : Immunofluorescence staining of CD29 was positive. C : Flow cytometric analysis showed that the positive rates of CD29, CD34, CD44, CD45, CD71, CD90, and CD133 were 81.56%, 0.10%, 88.77%, 0.17%, 10.02%, 98.43%, and 1.56%, respectively. D : Scanning electron microscopy (SEM) of MSCs showed a long spindle-shaped appearance with rich processes (1000×).

    Techniques Used: In Vitro, Cell Culture, Immunofluorescence, Staining, Flow Cytometry, Electron Microscopy

    32) Product Images from "MicroRNA-338 Inhibits Growth, Invasion and Metastasis of Gastric Cancer by Targeting NRP1 Expression"

    Article Title: MicroRNA-338 Inhibits Growth, Invasion and Metastasis of Gastric Cancer by Targeting NRP1 Expression

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0094422

    miR-338 decreases tumor growth and suppresses D-MVA by targeting NRP1 in vivo. (A) miR-338 expression in nude mouse tumors was measured using qRT-PCR. (B) Left panel: primary tumor growth after the orthotopic injection of 1×10 6 AGS cells with the forced expression of miR-338 or cont-miR, with or without NRP1 restoration. Right panel: a representative image of xenograft tumors from nude mice. (C) NRP1 expression in nude mouse tumors was detected by western blot. (D) NRP1 expression in nude mouse tumors was detected by immunohistochemical staining. The arrows indicate NRP1. (E) CD34staining revealed few patent vessels per field in the tumors that overexpressed miR-338; however, the vessel number was restored in the tumors that overexpressed NRP1. (F) Binary images of CD34 staining with lumens digitally filled show lower D-MVA in miR-338 tumor sections and D-MVA was restored in the tumors that overexpressed NRP1. (G) The D-MVA was decreased in nude mouse tumors that overexpressed miR-338; however, the D-MVA was restored in the tumors that overexpressed NRP1. The data represent the means±s.d.; * p
    Figure Legend Snippet: miR-338 decreases tumor growth and suppresses D-MVA by targeting NRP1 in vivo. (A) miR-338 expression in nude mouse tumors was measured using qRT-PCR. (B) Left panel: primary tumor growth after the orthotopic injection of 1×10 6 AGS cells with the forced expression of miR-338 or cont-miR, with or without NRP1 restoration. Right panel: a representative image of xenograft tumors from nude mice. (C) NRP1 expression in nude mouse tumors was detected by western blot. (D) NRP1 expression in nude mouse tumors was detected by immunohistochemical staining. The arrows indicate NRP1. (E) CD34staining revealed few patent vessels per field in the tumors that overexpressed miR-338; however, the vessel number was restored in the tumors that overexpressed NRP1. (F) Binary images of CD34 staining with lumens digitally filled show lower D-MVA in miR-338 tumor sections and D-MVA was restored in the tumors that overexpressed NRP1. (G) The D-MVA was decreased in nude mouse tumors that overexpressed miR-338; however, the D-MVA was restored in the tumors that overexpressed NRP1. The data represent the means±s.d.; * p

    Techniques Used: In Vivo, Expressing, Quantitative RT-PCR, Injection, Mouse Assay, Western Blot, Immunohistochemistry, Staining

    33) Product Images from "Transplantation of human matrix metalloproteinase-1 gene-modified bone marrow-derived mesenchymal stem cell attenuates CCL4-induced liver fibrosis in rats"

    Article Title: Transplantation of human matrix metalloproteinase-1 gene-modified bone marrow-derived mesenchymal stem cell attenuates CCL4-induced liver fibrosis in rats

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2018.3516

    Expression of molecules on cells by flow cytometry analysis. Cells were positive on (A) CD90, and (B) CD105, while negative on (C) CD14, (D) CD34, (E) CD45 and (F) CD79a, indicating that isolated cells were bone marrow-derived mesenchymal stem cells (BMSCs).
    Figure Legend Snippet: Expression of molecules on cells by flow cytometry analysis. Cells were positive on (A) CD90, and (B) CD105, while negative on (C) CD14, (D) CD34, (E) CD45 and (F) CD79a, indicating that isolated cells were bone marrow-derived mesenchymal stem cells (BMSCs).

    Techniques Used: Expressing, Flow Cytometry, Cytometry, Isolation, Derivative Assay

    34) Product Images from "ATP6L promotes metastasis of colorectal cancer by inducing epithelial‐mesenchymal transition, et al. ATP6L promotes metastasis of colorectal cancer by inducing epithelial‐mesenchymal transition"

    Article Title: ATP6L promotes metastasis of colorectal cancer by inducing epithelial‐mesenchymal transition, et al. ATP6L promotes metastasis of colorectal cancer by inducing epithelial‐mesenchymal transition

    Journal: Cancer Science

    doi: 10.1111/cas.14283

    Effect of ATP6L overexpression on necrosis and angiogenesis of harvested mouse tumor. A, H E photographs illustrating necrosis in mice tumor tissues derived from ATP6L‐transfected HCT116 cells and control cells, 40×. B, Quantitative analysis of the proportions of necrotic tissue in the largest section of tumor masses is shown as mean ± SD. C, Immunohistochemical staining for CD34 expression in harvested mouse tumor samples, 200×. Black arrows point to the microvessels in the tumor
    Figure Legend Snippet: Effect of ATP6L overexpression on necrosis and angiogenesis of harvested mouse tumor. A, H E photographs illustrating necrosis in mice tumor tissues derived from ATP6L‐transfected HCT116 cells and control cells, 40×. B, Quantitative analysis of the proportions of necrotic tissue in the largest section of tumor masses is shown as mean ± SD. C, Immunohistochemical staining for CD34 expression in harvested mouse tumor samples, 200×. Black arrows point to the microvessels in the tumor

    Techniques Used: Over Expression, Mouse Assay, Derivative Assay, Transfection, Immunohistochemistry, Staining, Expressing

    35) Product Images from "Low Intensity Pulsed Ultrasound Enhanced Mesenchymal Stem Cell Recruitment through Stromal Derived Factor-1 Signaling in Fracture Healing"

    Article Title: Low Intensity Pulsed Ultrasound Enhanced Mesenchymal Stem Cell Recruitment through Stromal Derived Factor-1 Signaling in Fracture Healing

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0106722

    MSCs characterization. ( A ) The expressions of selected surface markers on the isolated cells from BM of SD rats. This figure shows the expressions of mesenchymal stem cell markers (CD90 and CD44), endothelial cell marker (CD31) and hematopoietic cell markers (CD34 and CD45) on the isolated cell colonies. ( B ) Representative microphotograph of Alizarin Red S-stained cells isolated from BM of SD rats. White arrows indicate the obvious calcium deposition areas in the matrix. Scale bar = 100 µm. ( C ) Representative microphotograph of Oil Red O-stained cells isolated from BM of SD rats. The black arrows indicate the adipose-differentiated cells. The small red bubbles in cells are lipids. Scale bar = 100 µm.
    Figure Legend Snippet: MSCs characterization. ( A ) The expressions of selected surface markers on the isolated cells from BM of SD rats. This figure shows the expressions of mesenchymal stem cell markers (CD90 and CD44), endothelial cell marker (CD31) and hematopoietic cell markers (CD34 and CD45) on the isolated cell colonies. ( B ) Representative microphotograph of Alizarin Red S-stained cells isolated from BM of SD rats. White arrows indicate the obvious calcium deposition areas in the matrix. Scale bar = 100 µm. ( C ) Representative microphotograph of Oil Red O-stained cells isolated from BM of SD rats. The black arrows indicate the adipose-differentiated cells. The small red bubbles in cells are lipids. Scale bar = 100 µm.

    Techniques Used: Isolation, Marker, Staining

    36) Product Images from "Characteristics and Cardiomyogenic Potential of Rat Fetal Cardiac Progenitor Cells at Different Developmental Stage"

    Article Title: Characteristics and Cardiomyogenic Potential of Rat Fetal Cardiac Progenitor Cells at Different Developmental Stage

    Journal: Tissue Engineering and Regenerative Medicine

    doi: 10.1007/s13770-016-0016-z

    Flow cytometric analyses of rFCPCs of E12 and E15. rFCPCs at passages 1, 5, and 10 were strongly positive for CD29, CD73 and CD105, and negative for CD34 and CD45 in both E12 and E15
    Figure Legend Snippet: Flow cytometric analyses of rFCPCs of E12 and E15. rFCPCs at passages 1, 5, and 10 were strongly positive for CD29, CD73 and CD105, and negative for CD34 and CD45 in both E12 and E15

    Techniques Used: Flow Cytometry

    37) Product Images from "Human adipose-derived stem cells inhibit bioactivity of keloid fibroblasts"

    Article Title: Human adipose-derived stem cells inhibit bioactivity of keloid fibroblasts

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-018-0786-4

    Adipose-derived stem cell-conditioned medium (ADSC-CM) reduces angiogenesis in keloid explant culture. After culturing with ( b , d ) or without ( a , c ) ADSC-CM for 8 days, the keloid explants were sectioned and subjected to immunohistochemical analysis using antibodies against CD31 ( a , b ) and CD34 ( c , d ). Brown coloration indicates positive staining of related markers. The numbers of CD31-positive endothelial cells and CD34-positive microvascular endothelial cells were counted in three randomly selected fields for each sample under a microscope. Scale bars = 100 μm. * P
    Figure Legend Snippet: Adipose-derived stem cell-conditioned medium (ADSC-CM) reduces angiogenesis in keloid explant culture. After culturing with ( b , d ) or without ( a , c ) ADSC-CM for 8 days, the keloid explants were sectioned and subjected to immunohistochemical analysis using antibodies against CD31 ( a , b ) and CD34 ( c , d ). Brown coloration indicates positive staining of related markers. The numbers of CD31-positive endothelial cells and CD34-positive microvascular endothelial cells were counted in three randomly selected fields for each sample under a microscope. Scale bars = 100 μm. * P

    Techniques Used: Derivative Assay, Immunohistochemistry, Staining, Microscopy

    Characterization of human adipose-derived stem cells (ADSCs). a Flow cytometric characterization of ADSCs. ADSCs strongly expressed CD29, CD44, CD90, and CD105, and did not express CD31, CD34, or CD45. b ADSCs exhibited a fibroblast-like morphology. c Cells were induced to differentiate into adipocytes (left panel) and osteoblasts (right panel); scale bars = 100 μm
    Figure Legend Snippet: Characterization of human adipose-derived stem cells (ADSCs). a Flow cytometric characterization of ADSCs. ADSCs strongly expressed CD29, CD44, CD90, and CD105, and did not express CD31, CD34, or CD45. b ADSCs exhibited a fibroblast-like morphology. c Cells were induced to differentiate into adipocytes (left panel) and osteoblasts (right panel); scale bars = 100 μm

    Techniques Used: Derivative Assay, Flow Cytometry

    38) Product Images from "Transplantation of human matrix metalloproteinase-1 gene-modified bone marrow-derived mesenchymal stem cell attenuates CCL4-induced liver fibrosis in rats"

    Article Title: Transplantation of human matrix metalloproteinase-1 gene-modified bone marrow-derived mesenchymal stem cell attenuates CCL4-induced liver fibrosis in rats

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2018.3516

    Expression of molecules on cells by flow cytometry analysis. Cells were positive on (A) CD90, and (B) CD105, while negative on (C) CD14, (D) CD34, (E) CD45 and (F) CD79a, indicating that isolated cells were bone marrow-derived mesenchymal stem cells (BMSCs).
    Figure Legend Snippet: Expression of molecules on cells by flow cytometry analysis. Cells were positive on (A) CD90, and (B) CD105, while negative on (C) CD14, (D) CD34, (E) CD45 and (F) CD79a, indicating that isolated cells were bone marrow-derived mesenchymal stem cells (BMSCs).

    Techniques Used: Expressing, Flow Cytometry, Cytometry, Isolation, Derivative Assay

    39) Product Images from "Intraperitoneal but Not Intravenous Cryopreserved Mesenchymal Stromal Cells Home to the Inflamed Colon and Ameliorate Experimental Colitis"

    Article Title: Intraperitoneal but Not Intravenous Cryopreserved Mesenchymal Stromal Cells Home to the Inflamed Colon and Ameliorate Experimental Colitis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0033360

    Biological characterization of mesenchymal stromal cells. Phenotypic analysis of MSCs was carried out by flow cytometry, which revealed that BM- and AT-MSCs expressed the cell markers CD90 (Thy-1) and CD29, but did not express lineage markers such as CD45, CD11b and CD34 (A–F). Solid black lines show AT-MSC, dotted black lines show BM-MSC, and gray lines are isotype control. Functionally, MSCs have the capacity to form different cell lineages. AT- (G–I) and BM- (J–K) MSCs were able to differentiate into adipocytes (H, K) and osteocytes (I, L). These cells were used in subsequent experiments. Oil Red O (G–H, J–K) and Von Kossa (I, L) staining. Length bars represent 50 µm.
    Figure Legend Snippet: Biological characterization of mesenchymal stromal cells. Phenotypic analysis of MSCs was carried out by flow cytometry, which revealed that BM- and AT-MSCs expressed the cell markers CD90 (Thy-1) and CD29, but did not express lineage markers such as CD45, CD11b and CD34 (A–F). Solid black lines show AT-MSC, dotted black lines show BM-MSC, and gray lines are isotype control. Functionally, MSCs have the capacity to form different cell lineages. AT- (G–I) and BM- (J–K) MSCs were able to differentiate into adipocytes (H, K) and osteocytes (I, L). These cells were used in subsequent experiments. Oil Red O (G–H, J–K) and Von Kossa (I, L) staining. Length bars represent 50 µm.

    Techniques Used: Flow Cytometry, Cytometry, Staining

    40) Product Images from "Senescent peritoneal mesothelium induces a pro-angiogenic phenotype in ovarian cancer cells in vitro and in a mouse xenograft model in vivo"

    Article Title: Senescent peritoneal mesothelium induces a pro-angiogenic phenotype in ovarian cancer cells in vitro and in a mouse xenograft model in vivo

    Journal: Clinical & Experimental Metastasis

    doi: 10.1007/s10585-015-9753-y

    Magnitude of angiogenesis in tumors that developed upon i.p. injection of ovarian cancer cells alone or in combination with young and senescent HPMCs. The results derive from experiments as shown and described in Fig. 4 . Representative images showing the results of immunostaining for markers of the vascular endothelium, CD31 ( a ) and CD34 ( c ). CD31/CD34-positive cells display brown membrane and/or cytoplasm staining (some examples are marked with arrows ). Quantification of the brown-stained area reflecting the presence of CD31- ( b ) and CD34-positive cells ( d ). The results are expressed as a percentage, and the whole area of a specimen is considered as 100 %. The single asterisks indicate a significant difference as compared with the xenografts that developed in the presence of young HPMCs. The double asterisks indicate a significant difference as compared with the xenografts that developed in the absence of HPMCs. Experiments were performed with HPMCs from 6 different donors and with 6 pairs of animals (each pair received cancer cells and young or senescent HPMCs from the same donor)
    Figure Legend Snippet: Magnitude of angiogenesis in tumors that developed upon i.p. injection of ovarian cancer cells alone or in combination with young and senescent HPMCs. The results derive from experiments as shown and described in Fig. 4 . Representative images showing the results of immunostaining for markers of the vascular endothelium, CD31 ( a ) and CD34 ( c ). CD31/CD34-positive cells display brown membrane and/or cytoplasm staining (some examples are marked with arrows ). Quantification of the brown-stained area reflecting the presence of CD31- ( b ) and CD34-positive cells ( d ). The results are expressed as a percentage, and the whole area of a specimen is considered as 100 %. The single asterisks indicate a significant difference as compared with the xenografts that developed in the presence of young HPMCs. The double asterisks indicate a significant difference as compared with the xenografts that developed in the absence of HPMCs. Experiments were performed with HPMCs from 6 different donors and with 6 pairs of animals (each pair received cancer cells and young or senescent HPMCs from the same donor)

    Techniques Used: Injection, Immunostaining, Staining

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    Article Snippet: Although these HTSCs exhibit a similar morphology and characteristics to human testicular MSC-like cells [ ] and BM-MSCs [ ], these HTSCs are different from other known MSCs or MSC-like cells in that they initially coexpress both CD34 and CD73.

    Incubation:

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    Article Snippet: Endogenous peroxidase activity was blocked with 3% hydrogen peroxidase for 30 min at room temperature. .. Sections were incubated in humid chambers with a mouse LYVE-1 (a kind gift from Dr. David Jackson [ ]), CD34 (Santa Cruz, USA), VEGFR3 (a kind gift from Professor Kari Alitalo [ ]) and VEGFR2 (R & D, UK) antibodies o/n at 4°C. .. The samples were treated with biotin-labeled goat anti-mouse (Vector, Burlingame, USA), a biotin-labeled rabbit anti-rat (DAKO, Denmark) or biotin-labeled rabbit anti-mouse secondary antibodies, according to the nature of the primary antibodies.

    Isolation:

    Article Title: Thioredoxin-1 Protects Bone Marrow-Derived Mesenchymal Stromal Cells from Hyperoxia-Induced Injury In Vitro
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    Flow Cytometry:

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    Article Title: Transplanted Endothelial Progenitor Cells Improve Ischemia Muscle Regeneration in Mice by Diffusion Tensor MR Imaging
    Article Snippet: .. The results of flow cytometry showed the expression levels of CD34 (52.73%), CD133 (14.28%), and VEGF receptor 2 (61.63%) (Figures – ). .. These cells also expressed CD31 (3.26%), which is a specific marker of endothelial cell.

    Cytometry:

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    Article Snippet: .. At day 14, EPCs were identified by CD34, CD133, FLK-1 using flow cytometer. .. DiL-acLDL (red)/FITC-UEA-1 (green) dual staining cells were observed under the laser scanning confocal microscopy.

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    Expressing:

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    Article Snippet: .. The results of flow cytometry showed the expression levels of CD34 (52.73%), CD133 (14.28%), and VEGF receptor 2 (61.63%) (Figures – ). .. These cells also expressed CD31 (3.26%), which is a specific marker of endothelial cell.

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    Santa Cruz Biotechnology cd34
    Characterization of rat bone marrow-derived mesenchymal stromal cells (BMSCs). (a) The plastic-adherent cells demonstrated a homogeneous fibroblast-like and spindle-shaped morphology. Original magnification, ×100. (b) Adipogenic differentiation of BMSCs stained with oil red O. Original magnification, ×200. (c) Osteogenic differentiation of BM-MSCs stained with alizarin red. Original magnification, ×400. (d) FACS analysis demonstrated expression of markers attributed to BMSCs. The cells were devoid of hematopoietic cells as indicated by the lack of CD45 and <t>CD34.</t> The MSC-specific markers, CD29, CD44, CD73, CD105, and CD90 were strongly expressed on the cells.
    Cd34, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Characterization of rat bone marrow-derived mesenchymal stromal cells (BMSCs). (a) The plastic-adherent cells demonstrated a homogeneous fibroblast-like and spindle-shaped morphology. Original magnification, ×100. (b) Adipogenic differentiation of BMSCs stained with oil red O. Original magnification, ×200. (c) Osteogenic differentiation of BM-MSCs stained with alizarin red. Original magnification, ×400. (d) FACS analysis demonstrated expression of markers attributed to BMSCs. The cells were devoid of hematopoietic cells as indicated by the lack of CD45 and CD34. The MSC-specific markers, CD29, CD44, CD73, CD105, and CD90 were strongly expressed on the cells.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Thioredoxin-1 Protects Bone Marrow-Derived Mesenchymal Stromal Cells from Hyperoxia-Induced Injury In Vitro

    doi: 10.1155/2018/1023025

    Figure Lengend Snippet: Characterization of rat bone marrow-derived mesenchymal stromal cells (BMSCs). (a) The plastic-adherent cells demonstrated a homogeneous fibroblast-like and spindle-shaped morphology. Original magnification, ×100. (b) Adipogenic differentiation of BMSCs stained with oil red O. Original magnification, ×200. (c) Osteogenic differentiation of BM-MSCs stained with alizarin red. Original magnification, ×400. (d) FACS analysis demonstrated expression of markers attributed to BMSCs. The cells were devoid of hematopoietic cells as indicated by the lack of CD45 and CD34. The MSC-specific markers, CD29, CD44, CD73, CD105, and CD90 were strongly expressed on the cells.

    Article Snippet: The isolated primary BMSCs in this study have properties of mesenchymal stromal cells, according to the criteria of the International Society for Cellular Therapy (ISCT) [ ], such as being spindle shaped, plastic adherent, CD29+, CD44+, CD73+, CD105+, CD90+, CD45−, and CD34− and having multipotent differentiation ( ).

    Techniques: Derivative Assay, Staining, FACS, Expressing

    Adipose-derived stem cell-conditioned medium (ADSC-CM) reduces angiogenesis in keloid explant culture. After culturing with ( b , d ) or without ( a , c ) ADSC-CM for 8 days, the keloid explants were sectioned and subjected to immunohistochemical analysis using antibodies against CD31 ( a , b ) and CD34 ( c , d ). Brown coloration indicates positive staining of related markers. The numbers of CD31-positive endothelial cells and CD34-positive microvascular endothelial cells were counted in three randomly selected fields for each sample under a microscope. Scale bars = 100 μm. * P

    Journal: Stem Cell Research & Therapy

    Article Title: Human adipose-derived stem cells inhibit bioactivity of keloid fibroblasts

    doi: 10.1186/s13287-018-0786-4

    Figure Lengend Snippet: Adipose-derived stem cell-conditioned medium (ADSC-CM) reduces angiogenesis in keloid explant culture. After culturing with ( b , d ) or without ( a , c ) ADSC-CM for 8 days, the keloid explants were sectioned and subjected to immunohistochemical analysis using antibodies against CD31 ( a , b ) and CD34 ( c , d ). Brown coloration indicates positive staining of related markers. The numbers of CD31-positive endothelial cells and CD34-positive microvascular endothelial cells were counted in three randomly selected fields for each sample under a microscope. Scale bars = 100 μm. * P

    Article Snippet: The numbers of CD31+ and CD34+ vessels were evaluated in six randomly selected fields under the microscope.

    Techniques: Derivative Assay, Immunohistochemistry, Staining, Microscopy

    Characterization of human adipose-derived stem cells (ADSCs). a Flow cytometric characterization of ADSCs. ADSCs strongly expressed CD29, CD44, CD90, and CD105, and did not express CD31, CD34, or CD45. b ADSCs exhibited a fibroblast-like morphology. c Cells were induced to differentiate into adipocytes (left panel) and osteoblasts (right panel); scale bars = 100 μm

    Journal: Stem Cell Research & Therapy

    Article Title: Human adipose-derived stem cells inhibit bioactivity of keloid fibroblasts

    doi: 10.1186/s13287-018-0786-4

    Figure Lengend Snippet: Characterization of human adipose-derived stem cells (ADSCs). a Flow cytometric characterization of ADSCs. ADSCs strongly expressed CD29, CD44, CD90, and CD105, and did not express CD31, CD34, or CD45. b ADSCs exhibited a fibroblast-like morphology. c Cells were induced to differentiate into adipocytes (left panel) and osteoblasts (right panel); scale bars = 100 μm

    Article Snippet: The numbers of CD31+ and CD34+ vessels were evaluated in six randomly selected fields under the microscope.

    Techniques: Derivative Assay, Flow Cytometry

    Effect of VEGF-C overexpression on angiogenesis of orthotopic tumors . H E-staining of representative PC-3/VEGF-C (A, orthotopic; C, subcutaneous) tumors and PC-3/mock (B, orthotopic; D, subcutaneous) tumors. PC-3/VEGF-C tumors showed angiogenic morphology with a rich network of capillaries compared with PC-3/mock tumors. There were significantly more blood capillaries (CD34 positive, arrows) in the PC-3/VEGF-C tumors (E, 220 ± 15 μm/mm 2 , n = 29) compared with PC-3/mock tumors (F and G, 37 ± 6 μm/mm 2 , n = 24), p

    Journal: BMC Cancer

    Article Title: Overexpression of vascular endothelial growth factor C increases growth and alters the metastatic pattern of orthotopic PC-3 prostate tumors

    doi: 10.1186/1471-2407-9-362

    Figure Lengend Snippet: Effect of VEGF-C overexpression on angiogenesis of orthotopic tumors . H E-staining of representative PC-3/VEGF-C (A, orthotopic; C, subcutaneous) tumors and PC-3/mock (B, orthotopic; D, subcutaneous) tumors. PC-3/VEGF-C tumors showed angiogenic morphology with a rich network of capillaries compared with PC-3/mock tumors. There were significantly more blood capillaries (CD34 positive, arrows) in the PC-3/VEGF-C tumors (E, 220 ± 15 μm/mm 2 , n = 29) compared with PC-3/mock tumors (F and G, 37 ± 6 μm/mm 2 , n = 24), p

    Article Snippet: Sections were incubated in humid chambers with a mouse LYVE-1 (a kind gift from Dr. David Jackson [ ]), CD34 (Santa Cruz, USA), VEGFR3 (a kind gift from Professor Kari Alitalo [ ]) and VEGFR2 (R & D, UK) antibodies o/n at 4°C.

    Techniques: Over Expression, Staining

    Characterization of rat bone marrow-derived mesenchymal stromal cells (BMSCs). (a) The plastic-adherent cells demonstrated a homogeneous fibroblast-like and spindle-shaped morphology. Original magnification, ×100. (b) Adipogenic differentiation of BMSCs stained with oil red O. Original magnification, ×200. (c) Osteogenic differentiation of BM-MSCs stained with alizarin red. Original magnification, ×400. (d) FACS analysis demonstrated expression of markers attributed to BMSCs. The cells were devoid of hematopoietic cells as indicated by the lack of CD45 and CD34. The MSC-specific markers, CD29, CD44, CD73, CD105, and CD90 were strongly expressed on the cells.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Thioredoxin-1 Protects Bone Marrow-Derived Mesenchymal Stromal Cells from Hyperoxia-Induced Injury In Vitro

    doi: 10.1155/2018/1023025

    Figure Lengend Snippet: Characterization of rat bone marrow-derived mesenchymal stromal cells (BMSCs). (a) The plastic-adherent cells demonstrated a homogeneous fibroblast-like and spindle-shaped morphology. Original magnification, ×100. (b) Adipogenic differentiation of BMSCs stained with oil red O. Original magnification, ×200. (c) Osteogenic differentiation of BM-MSCs stained with alizarin red. Original magnification, ×400. (d) FACS analysis demonstrated expression of markers attributed to BMSCs. The cells were devoid of hematopoietic cells as indicated by the lack of CD45 and CD34. The MSC-specific markers, CD29, CD44, CD73, CD105, and CD90 were strongly expressed on the cells.

    Article Snippet: The isolated primary BMSCs in this study have properties of mesenchymal stromal cells, according to the criteria of the International Society for Cellular Therapy (ISCT) [ ], such as being spindle shaped, plastic adherent, CD29+, CD44+, CD73+, CD105+, CD90+, CD45−, and CD34− and having multipotent differentiation ( ).

    Techniques: Derivative Assay, Staining, FACS, Expressing