cd34 ve cadherin vegf  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    CD34 gp105 120 Recombinant Protein
    Description:
    CD34 gp105 120 Recombinant Protein for Ctrl
    Catalog Number:
    RP38972
    Price:
    None
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher cd34 ve cadherin vegf
    Expression of <t>CD34,</t> <t>VE-cadherin,</t> and <t>VEGF</t> protein under static culture and dynamic perfusion culture. (* p
    CD34 gp105 120 Recombinant Protein for Ctrl
    https://www.bioz.com/result/cd34 ve cadherin vegf/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd34 ve cadherin vegf - by Bioz Stars, 2021-04
    99/100 stars

    Images

    1) Product Images from "Dynamic Perfusion Culture of Human Outgrowth Endothelial Progenitor Cells on Demineralized Bone Matrix In Vitro"

    Article Title: Dynamic Perfusion Culture of Human Outgrowth Endothelial Progenitor Cells on Demineralized Bone Matrix In Vitro

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.897884

    Expression of CD34, VE-cadherin, and VEGF protein under static culture and dynamic perfusion culture. (* p
    Figure Legend Snippet: Expression of CD34, VE-cadherin, and VEGF protein under static culture and dynamic perfusion culture. (* p

    Techniques Used: Expressing

    The influence of dynamic perfusion culture on CD34/VE-cadherin/VEGF mRNA expression (* p
    Figure Legend Snippet: The influence of dynamic perfusion culture on CD34/VE-cadherin/VEGF mRNA expression (* p

    Techniques Used: Expressing

    Related Articles

    Flow Cytometry:

    Article Title: CpG island hypermethylation mediated by DNMT3A is a consequence of AML progression
    Article Snippet: PCR reactions were purified via spin columns (Macherey-Nagel PCR cleanup kit), and used as input for 20 uL Sanger sequencing reactions with 5 pmol forward primer, followed by capillary electrophoresis, which yielded unambiguous sequencing results for 44 of the 58 wells. .. On day 30, cells from these 44 wells were pooled according to genotype, counted, and a subset of the cells removed for analysis via flow cytometry for CD45-PerCPCy5.5 (eBioscience, 2D1), CD34-PE (as above), CD33-APC (eBioscience, clone WM-53, CD15-FITC (as above), CD11b-V450 (BD, clone ICRF44). .. The remaining cells were used for DNA and RNA extractions using the Qiagen QIAmp DNA Mini Kit and Zymo RNA Micro kits, respectively.

    Article Title: MUC1‐C drives myeloid leukaemogenesis and resistance to treatment by a survivin‐mediated mechanism, et al. MUC1‐C drives myeloid leukaemogenesis and resistance to treatment by a survivin‐mediated mechanism
    Article Snippet: Densitometry analysis was performed used image J software. .. 2.5 Analysis of intracellular protein expression by flow cytometry For β‐catenin expression ‐ isolated leukaemia CD34+ progenitors were permeabilized with a saponin‐based reagent (eBioscience). .. The cells were then stained with purified anti‐active β‐catenin (Millipore) for 1 hour followed by secondary labelling of the cells with FITC‐conjugated goat antimouse IgG and then analysis by flow cyometry.

    Article Title: The transcriptional architecture of early human hematopoiesis identifies multilevel control of lymphoid commitment
    Article Snippet: Lin- CB transduced with control or KD lentiviral constructs were harvested 72 hours post-transduction and stained for CD34 (CD34-APC, BD, 1:100). .. Live (SytoxBlue-, 1:104 , Life Technologies) GFP+ CD34+ cells were sorted on a FACSAria flow-cytometer and 3×104 –5×104 cells/mouse were injected intrafemorally. .. Mice were sacrificed 8–10 weeks post-transplantation, the injected femur and other bones were flushed separately in Iscove’s modified Dulbecco’s medium (IMDM) and cells were stained with the following antibodies (all from BD and dilution 1:100 unless otherwise specified): CD19 PE, GlyA PE(Beckman Coulter), CD45 PECy5 (Beckman Coulter), CD14 PECy7 (1:200, Beckman Coulter), CD33 APC, CD15 V450; or for assessment of all the steps of B cell differentiation with: CD19 PE, CD33 PECy5 (Beckman Coulter), CD34 PerCPE710 (eBiosciences), CD38 PECy7, CD10 APC, CD20 APCCy7, CD45RA BV (1:50, Biolegend).

    Cytometry:

    Article Title: CpG island hypermethylation mediated by DNMT3A is a consequence of AML progression
    Article Snippet: PCR reactions were purified via spin columns (Macherey-Nagel PCR cleanup kit), and used as input for 20 uL Sanger sequencing reactions with 5 pmol forward primer, followed by capillary electrophoresis, which yielded unambiguous sequencing results for 44 of the 58 wells. .. On day 30, cells from these 44 wells were pooled according to genotype, counted, and a subset of the cells removed for analysis via flow cytometry for CD45-PerCPCy5.5 (eBioscience, 2D1), CD34-PE (as above), CD33-APC (eBioscience, clone WM-53, CD15-FITC (as above), CD11b-V450 (BD, clone ICRF44). .. The remaining cells were used for DNA and RNA extractions using the Qiagen QIAmp DNA Mini Kit and Zymo RNA Micro kits, respectively.

    Article Title: MUC1‐C drives myeloid leukaemogenesis and resistance to treatment by a survivin‐mediated mechanism, et al. MUC1‐C drives myeloid leukaemogenesis and resistance to treatment by a survivin‐mediated mechanism
    Article Snippet: Densitometry analysis was performed used image J software. .. 2.5 Analysis of intracellular protein expression by flow cytometry For β‐catenin expression ‐ isolated leukaemia CD34+ progenitors were permeabilized with a saponin‐based reagent (eBioscience). .. The cells were then stained with purified anti‐active β‐catenin (Millipore) for 1 hour followed by secondary labelling of the cells with FITC‐conjugated goat antimouse IgG and then analysis by flow cyometry.

    Labeling:

    Article Title: High-Efficiency Lentiviral Transduction of Human CD34+ Cells in High-Density Culture with Poloxamer and Prostaglandin E2
    Article Snippet: CD34 expression in high-density CD34+ cell culture was evaluated with CD34 antibody (Clone 563, Becton Dickinson) 4 days after transduction. .. High-density CD34+ cells were labeled with cell proliferation dye (CellTrace FarRed, Thermo Fisher Scientific) without lentiviral transduction, and 7 days later, cell proliferation was evaluated by reduction in FarRed fluorescent color ( C). .. qPCR Genomic DNA was extracted from CD34+ cells in high-density culture 6–7 days after lentiviral transduction.

    Transduction:

    Article Title: High-Efficiency Lentiviral Transduction of Human CD34+ Cells in High-Density Culture with Poloxamer and Prostaglandin E2
    Article Snippet: CD34 expression in high-density CD34+ cell culture was evaluated with CD34 antibody (Clone 563, Becton Dickinson) 4 days after transduction. .. High-density CD34+ cells were labeled with cell proliferation dye (CellTrace FarRed, Thermo Fisher Scientific) without lentiviral transduction, and 7 days later, cell proliferation was evaluated by reduction in FarRed fluorescent color ( C). .. qPCR Genomic DNA was extracted from CD34+ cells in high-density culture 6–7 days after lentiviral transduction.

    Expressing:

    Article Title: MUC1‐C drives myeloid leukaemogenesis and resistance to treatment by a survivin‐mediated mechanism, et al. MUC1‐C drives myeloid leukaemogenesis and resistance to treatment by a survivin‐mediated mechanism
    Article Snippet: Densitometry analysis was performed used image J software. .. 2.5 Analysis of intracellular protein expression by flow cytometry For β‐catenin expression ‐ isolated leukaemia CD34+ progenitors were permeabilized with a saponin‐based reagent (eBioscience). .. The cells were then stained with purified anti‐active β‐catenin (Millipore) for 1 hour followed by secondary labelling of the cells with FITC‐conjugated goat antimouse IgG and then analysis by flow cyometry.

    Isolation:

    Article Title: MUC1‐C drives myeloid leukaemogenesis and resistance to treatment by a survivin‐mediated mechanism, et al. MUC1‐C drives myeloid leukaemogenesis and resistance to treatment by a survivin‐mediated mechanism
    Article Snippet: Densitometry analysis was performed used image J software. .. 2.5 Analysis of intracellular protein expression by flow cytometry For β‐catenin expression ‐ isolated leukaemia CD34+ progenitors were permeabilized with a saponin‐based reagent (eBioscience). .. The cells were then stained with purified anti‐active β‐catenin (Millipore) for 1 hour followed by secondary labelling of the cells with FITC‐conjugated goat antimouse IgG and then analysis by flow cyometry.

    Injection:

    Article Title: The transcriptional architecture of early human hematopoiesis identifies multilevel control of lymphoid commitment
    Article Snippet: Lin- CB transduced with control or KD lentiviral constructs were harvested 72 hours post-transduction and stained for CD34 (CD34-APC, BD, 1:100). .. Live (SytoxBlue-, 1:104 , Life Technologies) GFP+ CD34+ cells were sorted on a FACSAria flow-cytometer and 3×104 –5×104 cells/mouse were injected intrafemorally. .. Mice were sacrificed 8–10 weeks post-transplantation, the injected femur and other bones were flushed separately in Iscove’s modified Dulbecco’s medium (IMDM) and cells were stained with the following antibodies (all from BD and dilution 1:100 unless otherwise specified): CD19 PE, GlyA PE(Beckman Coulter), CD45 PECy5 (Beckman Coulter), CD14 PECy7 (1:200, Beckman Coulter), CD33 APC, CD15 V450; or for assessment of all the steps of B cell differentiation with: CD19 PE, CD33 PECy5 (Beckman Coulter), CD34 PerCPE710 (eBiosciences), CD38 PECy7, CD10 APC, CD20 APCCy7, CD45RA BV (1:50, Biolegend).

    Plasmid Preparation:

    Article Title: Production and First-in-Man Use of T Cells Engineered to Express a HSVTK-CD34 Sort-Suicide Gene
    Article Snippet: .. Plasmids and cell lines A gamma retroviral vector plasmid, encoding long terminal repeats from Myeloproliferative sarcoma virus (MPSV) and the leader 71 sequence from MESV and coding for a suicide/sort fusion gene comprising splice site corrected HSVTK fused to a truncated splice variant of human CD34 ( ) has been previously described and was produced by Geneart (Germany) along with two independent accessory plasmids encoding ecotropic env and gag/pol, plasmids. .. Transiently produced ecotropic retroviral supernatant was produced in 293T cells (from a qualified master cell bank) and filtered (0.45 um) before transduction of PG13 cells (ATCC, CRL-10686), a stable packaging line producing Gibbon Ape Leukaemia Virus (GALV) pseudotyped retroviral vector .

    Sequencing:

    Article Title: Production and First-in-Man Use of T Cells Engineered to Express a HSVTK-CD34 Sort-Suicide Gene
    Article Snippet: .. Plasmids and cell lines A gamma retroviral vector plasmid, encoding long terminal repeats from Myeloproliferative sarcoma virus (MPSV) and the leader 71 sequence from MESV and coding for a suicide/sort fusion gene comprising splice site corrected HSVTK fused to a truncated splice variant of human CD34 ( ) has been previously described and was produced by Geneart (Germany) along with two independent accessory plasmids encoding ecotropic env and gag/pol, plasmids. .. Transiently produced ecotropic retroviral supernatant was produced in 293T cells (from a qualified master cell bank) and filtered (0.45 um) before transduction of PG13 cells (ATCC, CRL-10686), a stable packaging line producing Gibbon Ape Leukaemia Virus (GALV) pseudotyped retroviral vector .

    Variant Assay:

    Article Title: Production and First-in-Man Use of T Cells Engineered to Express a HSVTK-CD34 Sort-Suicide Gene
    Article Snippet: .. Plasmids and cell lines A gamma retroviral vector plasmid, encoding long terminal repeats from Myeloproliferative sarcoma virus (MPSV) and the leader 71 sequence from MESV and coding for a suicide/sort fusion gene comprising splice site corrected HSVTK fused to a truncated splice variant of human CD34 ( ) has been previously described and was produced by Geneart (Germany) along with two independent accessory plasmids encoding ecotropic env and gag/pol, plasmids. .. Transiently produced ecotropic retroviral supernatant was produced in 293T cells (from a qualified master cell bank) and filtered (0.45 um) before transduction of PG13 cells (ATCC, CRL-10686), a stable packaging line producing Gibbon Ape Leukaemia Virus (GALV) pseudotyped retroviral vector .

    Produced:

    Article Title: Production and First-in-Man Use of T Cells Engineered to Express a HSVTK-CD34 Sort-Suicide Gene
    Article Snippet: .. Plasmids and cell lines A gamma retroviral vector plasmid, encoding long terminal repeats from Myeloproliferative sarcoma virus (MPSV) and the leader 71 sequence from MESV and coding for a suicide/sort fusion gene comprising splice site corrected HSVTK fused to a truncated splice variant of human CD34 ( ) has been previously described and was produced by Geneart (Germany) along with two independent accessory plasmids encoding ecotropic env and gag/pol, plasmids. .. Transiently produced ecotropic retroviral supernatant was produced in 293T cells (from a qualified master cell bank) and filtered (0.45 um) before transduction of PG13 cells (ATCC, CRL-10686), a stable packaging line producing Gibbon Ape Leukaemia Virus (GALV) pseudotyped retroviral vector .

    Mouse Assay:

    Article Title: Regulator of Calcineurin 1 (Rcan1) Is Required for the Development of Pulmonary Eosinophilia in Allergic Inflammation in Mice
    Article Snippet: Remaining cells were further separated into CD34+ and CD34− cell populations by cell sorting. .. CD34+ Lin-Sca-1− and CD34− Lin-Sca-1− cells from four mice (M1 to M4) were then subjected to PCR analysis for Rcan1 and GAPDH using Applied Biosystems Assays-on-Demand reagents according to the manufacturer's protocol. ..

    Polymerase Chain Reaction:

    Article Title: Regulator of Calcineurin 1 (Rcan1) Is Required for the Development of Pulmonary Eosinophilia in Allergic Inflammation in Mice
    Article Snippet: Remaining cells were further separated into CD34+ and CD34− cell populations by cell sorting. .. CD34+ Lin-Sca-1− and CD34− Lin-Sca-1− cells from four mice (M1 to M4) were then subjected to PCR analysis for Rcan1 and GAPDH using Applied Biosystems Assays-on-Demand reagents according to the manufacturer's protocol. ..

    Modification:

    Article Title: Three-Stage Ex Vivo Expansion of High-Ploidy Megakaryocytic Cells: Toward Large-Scale Platelet Production
    Article Snippet: .. Previously frozen mPB CD34+ HSPCs were seeded in either X-VIVO 10 or Iscove's modified Dulbecco's medium (IMDM)+20% BSA, insulin and transferin (BIT) (79% IMDM [Gibco], 20% BIT 9500 Serum Substitute [STEMCELL], 1% Glutamax [Gibco], 1 μg/mL low-density lipoproteins [Calbiochem]), pH 7.2, supplemented with cocktail “c” ( ) using IL-3 from R & D and SCF from Peprotech, and cultured at 5% O2 . ..

    Cell Culture:

    Article Title: Three-Stage Ex Vivo Expansion of High-Ploidy Megakaryocytic Cells: Toward Large-Scale Platelet Production
    Article Snippet: .. Previously frozen mPB CD34+ HSPCs were seeded in either X-VIVO 10 or Iscove's modified Dulbecco's medium (IMDM)+20% BSA, insulin and transferin (BIT) (79% IMDM [Gibco], 20% BIT 9500 Serum Substitute [STEMCELL], 1% Glutamax [Gibco], 1 μg/mL low-density lipoproteins [Calbiochem]), pH 7.2, supplemented with cocktail “c” ( ) using IL-3 from R & D and SCF from Peprotech, and cultured at 5% O2 . ..

    Article Title: Functionally distinct disease-associated fibroblast subsets in rheumatoid arthritis
    Article Snippet: .. Cell culture We sorted CD34– THY1– fibroblasts, CD34– THY1+ CDH11+ fibroblasts, and CD34+ THY1+ CDH11+ fibroblasts, and cultured them in DMEM supplemented with 10% FBS (Gemini), 2 mM l -glutamine, antibiotics (penicillin and streptomycin), and essential and nonessential amino ac ids (Life Technologies). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher cd34 ve cadherin vegf
    Expression of <t>CD34,</t> <t>VE-cadherin,</t> and <t>VEGF</t> protein under static culture and dynamic perfusion culture. (* p
    Cd34 Ve Cadherin Vegf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd34 ve cadherin vegf/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd34 ve cadherin vegf - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Expression of CD34, VE-cadherin, and VEGF protein under static culture and dynamic perfusion culture. (* p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Dynamic Perfusion Culture of Human Outgrowth Endothelial Progenitor Cells on Demineralized Bone Matrix In Vitro

    doi: 10.12659/MSM.897884

    Figure Lengend Snippet: Expression of CD34, VE-cadherin, and VEGF protein under static culture and dynamic perfusion culture. (* p

    Article Snippet: Relative mRNA levels of CD34/VE-cadherin/VEGF were quantified by quantitative reverse transcriptase real-time PCR (QRT-PCR) using cDNA obtained from the reverse transcription reactions as template, with a StepOne instrument (Applied Biosystems) and SYBR-Green PCR Master Mix (Applied Biosystems).

    Techniques: Expressing

    The influence of dynamic perfusion culture on CD34/VE-cadherin/VEGF mRNA expression (* p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Dynamic Perfusion Culture of Human Outgrowth Endothelial Progenitor Cells on Demineralized Bone Matrix In Vitro

    doi: 10.12659/MSM.897884

    Figure Lengend Snippet: The influence of dynamic perfusion culture on CD34/VE-cadherin/VEGF mRNA expression (* p

    Article Snippet: Relative mRNA levels of CD34/VE-cadherin/VEGF were quantified by quantitative reverse transcriptase real-time PCR (QRT-PCR) using cDNA obtained from the reverse transcription reactions as template, with a StepOne instrument (Applied Biosystems) and SYBR-Green PCR Master Mix (Applied Biosystems).

    Techniques: Expressing