Journal: STAR Protocols

Article Title: Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures

doi: 10.1016/j.xpro.2022.101447

Figure Lengend Snippet:

Article Snippet: Rat Monoclonal eFluor 450 anti-mouse CD31 (PECAM-1) (1:200) , Thermo Fisher Scientific , Cat # 48-0311-82 RRID: AB_10598807.

Techniques: Recombinant, Lysis, Blocking Assay, Software, Cell Culture, Hood, Incubation, Hybridization

Journal: STAR Protocols

Article Title: Protocols for endothelial cell isolation from mouse tissues: brain, choroid, lung, and muscle

doi: 10.1016/j.xpro.2021.100508

Figure Lengend Snippet: EC isolation from mouse brain (A) Detailed scheme illustrating isolation of ECs from brain. (B) Representative FACS plots for the gating strategy to sort ECs from brain based on sorting live/CD45 - /CD31 + cells.

Article Snippet: Centrifuge the cell suspension at 250 g for 5 min, remove the supernatant. b. Resuspend the pellet in 0.5 mL Wash Buffer-based staining solution containing: i. CD31 (PECAM-1) Monoclonal Antibody (390), e.g.: CD31-FITC (Thermo Fisher Scientific, Cat#11-0311-82; 1:300). ii.

Techniques: Isolation

Journal: STAR Protocols

Article Title: Protocols for endothelial cell isolation from mouse tissues: brain, choroid, lung, and muscle

doi: 10.1016/j.xpro.2021.100508

Figure Lengend Snippet: EC isolation from mouse choroid (A) Detailed scheme illustrating isolation of ECs from choroid. (B) Representative FACS plots for the gating strategy to sort ECs from choroid based on sorting live/CD45 - /CD31 + cells.

Article Snippet: Centrifuge the cell suspension at 250 g for 5 min, remove the supernatant. b. Resuspend the pellet in 0.5 mL Wash Buffer-based staining solution containing: i. CD31 (PECAM-1) Monoclonal Antibody (390), e.g.: CD31-FITC (Thermo Fisher Scientific, Cat#11-0311-82; 1:300). ii.

Techniques: Isolation

Journal: STAR Protocols

Article Title: Protocols for endothelial cell isolation from mouse tissues: brain, choroid, lung, and muscle

doi: 10.1016/j.xpro.2021.100508

Figure Lengend Snippet: EC isolation from mouse lung (A) Detailed scheme illustrating isolation of ECs from lung. (B) Representative FACS plots for the gating strategy to sort ECs from lung based on sorting live/CD45 - /CD31 + cells.

Article Snippet: Centrifuge the cell suspension at 250 g for 5 min, remove the supernatant. b. Resuspend the pellet in 0.5 mL Wash Buffer-based staining solution containing: i. CD31 (PECAM-1) Monoclonal Antibody (390), e.g.: CD31-FITC (Thermo Fisher Scientific, Cat#11-0311-82; 1:300). ii.

Techniques: Isolation

Journal: STAR Protocols

Article Title: Protocols for endothelial cell isolation from mouse tissues: brain, choroid, lung, and muscle

doi: 10.1016/j.xpro.2021.100508

Figure Lengend Snippet: EC isolation from mouse muscle (A) Detailed scheme illustrating isolation of ECs from muscle. (B and C) Representative FACS plots for the gating strategy to sort ECs from EDL (B) and soleus (C) muscle based on sorting live/CD45 - /CD31 + cells.

Article Snippet: Centrifuge the cell suspension at 250 g for 5 min, remove the supernatant. b. Resuspend the pellet in 0.5 mL Wash Buffer-based staining solution containing: i. CD31 (PECAM-1) Monoclonal Antibody (390), e.g.: CD31-FITC (Thermo Fisher Scientific, Cat#11-0311-82; 1:300). ii.

Techniques: Isolation

Journal: STAR Protocols

Article Title: Protocols for endothelial cell isolation from mouse tissues: brain, choroid, lung, and muscle

doi: 10.1016/j.xpro.2021.100508

Figure Lengend Snippet:

Article Snippet: Centrifuge the cell suspension at 250 g for 5 min, remove the supernatant. b. Resuspend the pellet in 0.5 mL Wash Buffer-based staining solution containing: i. CD31 (PECAM-1) Monoclonal Antibody (390), e.g.: CD31-FITC (Thermo Fisher Scientific, Cat#11-0311-82; 1:300). ii.

Techniques: Recombinant, Knock-Out, Software, Transferring