Journal: Archives of Toxicology

Article Title: A hair-follicle reconstructed in vitro immunocompetent skin model for prediction of the sensitizing potential of chemicals

doi: 10.1007/s00204-025-04130-z

Figure Lengend Snippet: Characterization of ImmuSkin-MT, ImmuSkin-M, and ImmuSkin-T. Skin models were subjected to H&E and immunofluorescence staining against loricrin as a marker for differentiated keratinocytes (red) and CD1a as a specific DC marker (green). DAPI (blue) was used as a nucleus counter-staining. Carboxyfluorescein succinimidyl ester (CFSE, green) is an indicator of T-lymphocytes indicated the absence of T-cells in the epidermis, dermis, and the immune layer. The white dotted line indicates the boundary between epidermis and dermis. In some pictures from immunofluorescence staining, a membrane of the transwell insert is presented in a bright yellow-green strip. Images were captured at a magnification of 20x. Scale bar = 100 µm. n = 3. The schematic part of this figure was created with BioRender.com

Article Snippet: On day 6, CD1a + MoLCs were magnetically labeled and isolated using CD1a MicroBeads, human (Miltenyi Biotech, Bergisch Gladbach, Germany).

Techniques: Immunofluorescence, Staining, Marker, Membrane, Stripping Membranes

Journal: Archives of Toxicology

Article Title: A hair-follicle reconstructed in vitro immunocompetent skin model for prediction of the sensitizing potential of chemicals

doi: 10.1007/s00204-025-04130-z

Figure Lengend Snippet: Influence of cocultivation on the migration and maturation of MoLCs and the proliferation of T-cells. Cells harvested from the lower chamber of each ImmuSkin variant were subjected to flow cytometry analysis and stained with anti-CD1a-, anti-CD86- and anti CD4-antibodies. a Representative dot plots show the populations of T-lymphocytes and MoLCs from each ImmuSkin type. The upper left side of the quadrant represents T-lymphocytes, while MoLCs are shown in the lower right side of the quadrant. Moreover, MoLC migration ( b ), MoLC maturation ( c ) and T-cell proliferation ( d ) were measured. The black bar represents immune cells from ImmuSkin-M, the striped bar represents immune cells from ImmuSkin-T, while the gray bar represents immune cells from ImmuSkin-MT. Data are presented as mean ± SD, and statistical significance is indicated as * p -value ≤ 0.05, ** p -value ≤ 0.01, *** p -value ≤ 0.001. A paired t-test was used for the statistical analysis. n = 3. The schematic part of this figure was created with BioRender.com

Article Snippet: On day 6, CD1a + MoLCs were magnetically labeled and isolated using CD1a MicroBeads, human (Miltenyi Biotech, Bergisch Gladbach, Germany).

Techniques: Migration, Variant Assay, Flow Cytometry, Staining