cd163  (Bioss)

Journal: Frontiers in Microbiology

Article Title: Porcine peritoneal macrophages are susceptible to porcine reproductive and respiratory syndrome virus infection

doi: 10.3389/fmicb.2024.1505900

Figure Lengend Snippet: Expression of surface receptors. Freshly isolated PAMs and PPMs were analyzed by flow cytometry for the expression of CD172a, CD14, CD169, and CD163 proteins. (A) Percentage of cells individually expressing each of the examined markers. (B) Percentage of cells co-expressing CD163 and CD169 receptors. (C) MFI of the cell markers. The experiments were conducted using cells collected from three different pigs. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.

Article Snippet: The goat anti-human CD163 polyclonal antibody (R&D Systems; clone AF1407) was used for the receptor-blocking assay.

Techniques: Expressing, Isolation, Flow Cytometry

Journal: Frontiers in Microbiology

Article Title: Porcine peritoneal macrophages are susceptible to porcine reproductive and respiratory syndrome virus infection

doi: 10.3389/fmicb.2024.1505900

Figure Lengend Snippet: Expression of CD163 and CD169 in PRRSV-infected cells. Freshly isolated cells were infected with the PRRSV isolate RFLP-144 at an MOI of 2. At 24 hpi, cells were stained with antibodies against CD163, CD169, and PRRSV-N protein and analyzed by flow cytometry. Cells were first gated for PRRSV + populations and subsequently analyzed for CD163 and CD169 expression within the PRRSV + population. (A) Representative gating strategy. (B) Percentage of different cell populations within the PRRSV + cells. (C) MFI of PRRSV-N protein expression in different cell populations. Experiments were performed using cells from three different pigs. ** p ≤ 0.01.

Article Snippet: The goat anti-human CD163 polyclonal antibody (R&D Systems; clone AF1407) was used for the receptor-blocking assay.

Techniques: Expressing, Infection, Isolation, Staining, Flow Cytometry

Journal: Frontiers in Microbiology

Article Title: Porcine peritoneal macrophages are susceptible to porcine reproductive and respiratory syndrome virus infection

doi: 10.3389/fmicb.2024.1505900

Figure Lengend Snippet: Cultured PPMs are more susceptible to PRRSV. Freshly isolated or 24-h cultured PPMs and PAMs were inoculated with the PRRSV isolate RFLP-144 at an MOI of 2. At 24 hpi, cells were analyzed for the expression of viral N protein and the cellular markers CD14, CD163, and CD169 by flow cytometry. (A) Frequency of PRRSV-infected cells. (B) Frequency of cells expressing the indicated cellular markers. (C) MFI of the indicated markers. The experiments were conducted using cells from three different pigs. PAM/PPM, Freshly isolated cells; cPAM/cPPM, Cells cultured for 24 h before infection. * p ≤ 0.05.

Article Snippet: The goat anti-human CD163 polyclonal antibody (R&D Systems; clone AF1407) was used for the receptor-blocking assay.

Techniques: Cell Culture, Isolation, Expressing, Flow Cytometry, Infection

Journal: Frontiers in Microbiology

Article Title: Porcine peritoneal macrophages are susceptible to porcine reproductive and respiratory syndrome virus infection

doi: 10.3389/fmicb.2024.1505900

Figure Lengend Snippet: Infection of PPMs is dependent on CD163. PAMs and PPMs were cultured for 24 h and incubated with anti-human CD163 polyclonal antibody for 1 h prior to infection with PRRSV FL12 at an MOI of 2. Cells without antibody treatment (No Ab) were used as controls. At 24 hpi, cells were fixed and stained with an antibody specific to the viral N protein to detect infected cells. (A) Representative images showing PRRSV-infected cells (green). Cell nuclei were counterstained with DAPI (blue). Scale bar = 100 μm. (B) Percentage of PRRSV-positive cells determined by flow cytometry. The experiments were conducted using cells from three different pigs. ** p ≤ 0.01, *** p ≤ 0.001.

Article Snippet: The goat anti-human CD163 polyclonal antibody (R&D Systems; clone AF1407) was used for the receptor-blocking assay.

Techniques: Infection, Cell Culture, Incubation, Staining, Flow Cytometry

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: CD163 immunostaining showed that ( A ) PDAC Tumor cells were negative (IHCx40) ( B ) High expressions of CD163 in TAM in PDAC (IHCx200) ( C ) High expressions of CD163 in TAM in PDAC (IHCx400) ( D ) Negative expressions in control pancreatic tissue (IHCx200)

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques: Immunostaining, Control

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: Comparison between PDAC and control groups regarding CD163 staining

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques: Comparison, Control

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: High H score values of CD163 staining showed significant associations with ( A ) higher histological grade ( p < 0.026) ( B ) T3 tumor stage ( p < 0.002) ( C ) advanced stage group ( p < 0.001) ( D ) larger tumor size ( p < 0.002) ( E ) Significant direct correlation between high mean H score value of CD163 staining with larger tumor size ( p < 0.031)

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques: Staining

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: Relationships between HOXA9 and CD163 in PDAC ( n = 98)

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques:

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: Shorter overall survival by univariate survival analysis of PDAC cases showed significant associations with ( A ) Positive virology (HCV infection) ( P < 0.040) ( B ) high direct bilirubin ( P < 0.046) ( C ) larger tumor size ( P < 0.046) ( D ) advanced stage ( P < 0.034) (( E ) high median H score of HOXA9 ( P < 0.015) and CD163 ( P < 0.042)

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques: Infection

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: Multivariate COX regression analysis for the parameters affecting overall survival

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques: Infection

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: Multivariate COX regression analysis for the parameters affecting overall survival (continuous variables)

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques:

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: Univariate and multivariate COX regression analysis for the parameters affecting overall survival in negative Virology (HCV) cases

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques:

Journal: Diagnostic Pathology

Article Title: HOXA9 and CD163 potentiate pancreatic ductal adenocarcinoma progression

doi: 10.1186/s13000-024-01563-5

Figure Lengend Snippet: Univariate overall survival of PDAC cases (53 cases)

Article Snippet: Immunostaining was done using an IgG anti- HOXA9 rabbit’s polyclonal antibody (0.1 mL concentrated and diluted 1:150) (Chongqing Biospes Co., Ltd, China. catalog# YPA2228) and Rabbit polyclonal antibody against CD163 (Chongqing Biospes Co., Ltd, China. cat. # YPA1450), 100 ul concentrated with dilution of 1:100 as primary antibodies.

Techniques: Infection

Journal: Stem Cell Research & Therapy

Article Title: Human uncultured adipose-derived stromal vascular fraction shows therapeutic potential against osteoarthritis in immunodeficient rats via direct effects of transplanted M2 macrophages

doi: 10.1186/s13287-024-03946-3

Figure Lengend Snippet: A Immunohistochemical analysis of F4/80 in synovial tissue showing representative samples at 4 and 8 weeks after DMM. The sections were counterstained with hematoxylin. Scale bar = 100 μm. B F4/80 scores in each group (n = 5/group). C Immunohistochemical analysis of CD86 in synovial tissue showing representative samples at 4 and 8 weeks after DMM. The sections were counterstained with hematoxylin. Scale bar = 100 μm. D Immunohistochemical analysis of CD163 in synovial tissue showing representative samples at 4 and 8 weeks after DMM. The sections were counterstained with hematoxylin. Scale bar = 100 μm. ( E ) CD86/CD163 expression ratio (M1/M2 ratio) in each group (n = 5/group). SVF, stromal vascular fraction; ADSC, adipose-derived stromal cell; DMM, destabilization of the medial meniscus; MΦ, macrophages

Article Snippet: The samples were incubated overnight at 4 °C with a conjugated primary antibody against F4/80 (1:100; orb466169-FITC, Biorbyt), conjugated primary antibody against CD163 (1:200; BS-2527R-A594, Bioss), and primary anti-mouse antibody against hNA (1:500; ab191181, Abcam).

Techniques: Immunohistochemical staining, Expressing, Derivative Assay

Journal: Stem Cell Research & Therapy

Article Title: Human uncultured adipose-derived stromal vascular fraction shows therapeutic potential against osteoarthritis in immunodeficient rats via direct effects of transplanted M2 macrophages

doi: 10.1186/s13287-024-03946-3

Figure Lengend Snippet: A Immunofluorescence analysis of F4/80, CD163, and hNA in synovial tissue of the rats at 4 weeks after DMM showing representative triple immunostaining of F4/80 (green), CD163 (red), and hNA (violet) in each group. Scale bar = 20 μm. B Comparison of the ratio of CD163- and hNA-positive cells in the F4/80-positive cells via immunofluorescence staining (n = 5/group). SVF, stromal vascular fraction; ADSC, adipose-derived stromal cell; hNA, human nuclear antigen

Article Snippet: The samples were incubated overnight at 4 °C with a conjugated primary antibody against F4/80 (1:100; orb466169-FITC, Biorbyt), conjugated primary antibody against CD163 (1:200; BS-2527R-A594, Bioss), and primary anti-mouse antibody against hNA (1:500; ab191181, Abcam).

Techniques: Immunofluorescence, Triple Immunostaining, Comparison, Staining, Derivative Assay

Journal: Stem Cell Research & Therapy

Article Title: Human uncultured adipose-derived stromal vascular fraction shows therapeutic potential against osteoarthritis in immunodeficient rats via direct effects of transplanted M2 macrophages

doi: 10.1186/s13287-024-03946-3

Figure Lengend Snippet: A Flow cytometry analysis revealing the proportion of CD163-positive cells in the SVF (n = 5). B Setup of the separated pellet co-culture system. Groups including ADSC, M2Φ, and SVF were established, with each administered cell type in membrane plates and OA chondrocytes in 15 mL tubes. A control group was established with no cells in membrane plates and only OA chondrocytes in 15-mL tubes. C Gross photographs and safranin-O staining of the resulting pellets in each group. D Comparison of pellet size among each group (n = 5/group). E Analysis of TGF-β, IL-10, and MMP-13 levels in the supernatant following coculture with ADSC, M2Φ, and SVF and the chondrocyte (n = 5/group). SVF, stromal vascular fraction; M2Φ, M2 macrophages; ADSC, adipose-derived stromal cell; TGF-β, transforming growth factor-β; IL-10, interleukin-10; MMP-13, matrix metalloproteinase 13

Article Snippet: The samples were incubated overnight at 4 °C with a conjugated primary antibody against F4/80 (1:100; orb466169-FITC, Biorbyt), conjugated primary antibody against CD163 (1:200; BS-2527R-A594, Bioss), and primary anti-mouse antibody against hNA (1:500; ab191181, Abcam).

Techniques: Flow Cytometry, Co-Culture Assay, Membrane, Control, Staining, Comparison, Derivative Assay