nk92 cd16 nk cells (ATCC)


Structured Review
![( A – C ) Cell viability (MTT) assays of ( A ) SKOV3, ( B ) SNU-119, and ( C ) SNU-251 cell lines treated with various samfenet concentrations for 72 h. Three independently repeated experiments were performed with similar results. ( D – E ) The NK cytotoxicity assay in the presence or absence of samfenet was analyzed using CFSE-7AAD assay. Target cells [( D ) SKOV3, ( E ) SNU-119, and ( F ) SNU-251] and effector cells <t>(NK92-CD16</t> cells) were co-cultured at 1:1, 5:1, and 10:1 E/T ratios for 4 h with 5 mg/mL of samfenet. Three independently repeated experiments were performed with similar results. Error bars represent the standard deviations of three independent experiments. Student’s t -test between NK92-CD16 and Samfenet+NK92-CD16: * p < 0.05, ** p < 0.01. Abbreviation: ns, not significant. ( G ) Colony formation assay in the presence or absence of samfenet and NK92-CD16 with or without CD16 blocking. Target cells (SKOV3, SNU-119, SNU-251) and effector cells (NK92-CD16 cells) were co-cultured at 10:1 E/T ratio for 4 h with 400 ug/mL of samfenet. The photographs were taken 10 days after culture. Abbreviation: Samf: Samfenet; NK: NK92-CD16; aCD16: CD16 antibody.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5925/pmc11545925/pmc11545925__ijms-25-11733-g002.jpg)
Nk92 Cd16 Nk Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Trastuzumab-Mediated Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) Enhances Natural Killer Cell Cytotoxicity in HER2-Overexpressing Ovarian Cancer"
Article Title: Trastuzumab-Mediated Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) Enhances Natural Killer Cell Cytotoxicity in HER2-Overexpressing Ovarian Cancer
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms252111733
![... and ( F ) SNU-251] and effector cells (NK92-CD16 cells) were co-cultured at 1:1, 5:1, and 10:1 ... ( A – C ) Cell viability (MTT) assays of ( A ) SKOV3, ( B ) SNU-119, and ( C ) SNU-251 cell lines treated with various samfenet concentrations for 72 h. Three independently repeated experiments were performed with similar results. ( D – E ) The NK cytotoxicity assay in the presence or absence of samfenet was analyzed using CFSE-7AAD assay. Target cells [( D ) SKOV3, ( E ) SNU-119, and ( F ) SNU-251] and effector cells (NK92-CD16 cells) were co-cultured at 1:1, 5:1, and 10:1 E/T ratios for 4 h with 5 mg/mL of samfenet. Three independently repeated experiments were performed with similar results. Error bars represent the standard deviations of three independent experiments. Student’s t -test between NK92-CD16 and Samfenet+NK92-CD16: * p < 0.05, ** p < 0.01. Abbreviation: ns, not significant. ( G ) Colony formation assay in the presence or absence of samfenet and NK92-CD16 with or without CD16 blocking. Target cells (SKOV3, SNU-119, SNU-251) and effector cells (NK92-CD16 cells) were co-cultured at 10:1 E/T ratio for 4 h with 400 ug/mL of samfenet. The photographs were taken 10 days after culture. Abbreviation: Samf: Samfenet; NK: NK92-CD16; aCD16: CD16 antibody.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5925/pmc11545925/pmc11545925__ijms-25-11733-g002.jpg)
Figure Legend Snippet: ( A – C ) Cell viability (MTT) assays of ( A ) SKOV3, ( B ) SNU-119, and ( C ) SNU-251 cell lines treated with various samfenet concentrations for 72 h. Three independently repeated experiments were performed with similar results. ( D – E ) The NK cytotoxicity assay in the presence or absence of samfenet was analyzed using CFSE-7AAD assay. Target cells [( D ) SKOV3, ( E ) SNU-119, and ( F ) SNU-251] and effector cells (NK92-CD16 cells) were co-cultured at 1:1, 5:1, and 10:1 E/T ratios for 4 h with 5 mg/mL of samfenet. Three independently repeated experiments were performed with similar results. Error bars represent the standard deviations of three independent experiments. Student’s t -test between NK92-CD16 and Samfenet+NK92-CD16: * p < 0.05, ** p < 0.01. Abbreviation: ns, not significant. ( G ) Colony formation assay in the presence or absence of samfenet and NK92-CD16 with or without CD16 blocking. Target cells (SKOV3, SNU-119, SNU-251) and effector cells (NK92-CD16 cells) were co-cultured at 10:1 E/T ratio for 4 h with 400 ug/mL of samfenet. The photographs were taken 10 days after culture. Abbreviation: Samf: Samfenet; NK: NK92-CD16; aCD16: CD16 antibody.
Techniques Used: Cytotoxicity Assay, Cell Culture, Colony Assay, Blocking Assay

Figure Legend Snippet: ( A ) Treatment schedule for main in vivo efficacy testing using HER2-overexpressing PDTX model (PDTX 18-4). ( B ) The average relative tumor growth of HER2-overexpressing PDTX 18-4 tumors, which were treated with indicated drugs, in the preliminary in vivo test using primary NK cells. Error bars represent the standard error of the mean (SEM) of two tumors per group. ( C ) The average tumor volume of HER2-overexpressing PDTX 18-4 tumors, which were treated with indicated drugs, in the main in vivo test using NK92-CD16 cells. Error bars represent the SEM of five tumors per group. Student’s t -test: * p < 0.05. Abbreviation: ns, not significant. ( D ) Tumor growth curves of individual mice in the indicated treatment groups of the HER2-overexpressing PDTX 18-4 model. ( E ) Average body weight of mice with indicated groups. Error bars represent the standard deviations of five mice per group. ( F ) Gross harvested tumor. ( G ) TUNEL assay using PDTX 18-4 xenografted tumor after sacrifice. Staining images per group are shown, and bar graphs represent the average number of apoptotic cells per group in five random, non-overlapping fields at 400× magnification. Data are presented as mean ± SD. Student’s t -test: *** p < 0.001. Abbreviation: Con: control; Samf: samfenet; NK: NK92-CD16; Combi: combination. ( H ) Immunohistochemistry for human leukocyte common antigen (LCA) using PDTX 18-4 xenografted tumor after sacrifice. Staining images per group are shown, and bar graph represents the average number of LCA-positive cells per group in five random, non-overlapping fields at 400× magnification. Data are presented as mean ± SD. Student’s t -test: ** p < 0.01. Abbreviation: Con: control; Samf: samfenet; NK: NK92-CD16; Combi: combination.
Techniques Used: In Vivo, TUNEL Assay, Staining, Control, Immunohistochemistry