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antibodies against il 5rα  (R&D Systems)


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    R&D Systems antibodies against il 5rα
    Antibodies Against Il 5rα, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd125/Human+IL-5+R+alpha%2FCD125+Antibody/pm41188935-77-11-16
    Average 92 stars, based on 6 article reviews
    antibodies against il 5rα - by Bioz Stars, 2026-10
    92/100 stars

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    other:

    Article Title: GM-CSF differentiation of human monocytes stabilizes macrophage state via oxidative signaling
    Article Snippet: Panel 1: CD11b-BV421 (Biolegend, 301323), CD32-PECy7 (Biolegend, 303213), CD36-APC (Biolegend, 336207), HLA-DR-BV510 (Biolegend, 307645) Panel 2: CD11b-BV421 (Biolegend, 301323), CD274-PE (Biolegend, 329705), CD86-APC (Biolegend, 305411), CD64-BV510 (Biolegend, 305027) Additional antibodies utilized in this study included CD36-PE (Biolegend, 336205), CD206-PE (Biolegend, 321105), CD123 (Biolegend, 306005), CD125 (R&D Systems, FAB253P), CD115-BV421 (Biolegend, 347321), CD116-PE (Biolegend, 305908).



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    IL-5 induced Gαi1/3 association with activated IL-5 receptor and Gab1. WT and Gαi1/3 DKO MEFs were treated with IL-5 (25 ng/mL) for 5 min, the association between <t>IL-5Rα,</t> Gab1, and Gαi1/3 was tested by co-ip assay (A) . EoL-1 cells were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 5 min, the association between IL-5Rα, Gab1, and Gαi1/3 was tested by co-ip (B) , and processed for confocal microscopy to detect the presence of Gαi1, Gαi3, and IL-5Rα (C) . Bar = 1 μm.
    Il 5rα, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cd125 biotinstreptavidin bv421  (Miltenyi Biotec)
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    IL-5 induced Gαi1/3 association with activated IL-5 receptor and Gab1. WT and Gαi1/3 DKO MEFs were treated with IL-5 (25 ng/mL) for 5 min, the association between <t>IL-5Rα,</t> Gab1, and Gαi1/3 was tested by co-ip assay (A) . EoL-1 cells were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 5 min, the association between IL-5Rα, Gab1, and Gαi1/3 was tested by co-ip (B) , and processed for confocal microscopy to detect the presence of Gαi1, Gαi3, and IL-5Rα (C) . Bar = 1 μm.
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    antibodies against il 5rα  (R&D Systems)
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    IL-5 induced Gαi1/3 association with activated IL-5 receptor and Gab1. WT and Gαi1/3 DKO MEFs were treated with IL-5 (25 ng/mL) for 5 min, the association between <t>IL-5Rα,</t> Gab1, and Gαi1/3 was tested by co-ip assay (A) . EoL-1 cells were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 5 min, the association between IL-5Rα, Gab1, and Gαi1/3 was tested by co-ip (B) , and processed for confocal microscopy to detect the presence of Gαi1, Gαi3, and IL-5Rα (C) . Bar = 1 μm.
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    IL-5 induced Gαi1/3 association with activated IL-5 receptor and Gab1. WT and Gαi1/3 DKO MEFs were treated with IL-5 (25 ng/mL) for 5 min, the association between <t>IL-5Rα,</t> Gab1, and Gαi1/3 was tested by co-ip assay (A) . EoL-1 cells were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 5 min, the association between IL-5Rα, Gab1, and Gαi1/3 was tested by co-ip (B) , and processed for confocal microscopy to detect the presence of Gαi1, Gαi3, and IL-5Rα (C) . Bar = 1 μm.
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    p stat5  (Bioss)
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    Gαi1/3 knockdown inhibits <t>STAT5</t> activation, differentiation and growth by IL-5 in EoL-1 cells. Stable EoL-1 cells, expressing the scramble control shRNA (“C-shRNA”), Gαi1 shRNA and Gαi3 shRNA, were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 24 hours, and were tested by Western blotting of listed proteins (A, E) . Relative expression of listed genes (24 hours after GM-CSF and IL-5 treatment) was shown (B, C) ; In each experiment, n=3 (three replicated wells). Cells were further cultured for the times indicated, cell proliferation were tested (F, G) , with cell viability tested by CCK-8 assay (D) . Bar = 100 μm. Blotting quantification was performed from five replicate blot data, data of all repeated experiments were pulled together to calculate mean ± SD. *P <0.05 (B, E, G) . *P <0.05 vs. “Control” treatment in “C-shRNA” EoL-1 cells (C) . # P <0.05 vs. GM-CSF and IL-5 treatment in “C-shRNA” EoL-1 cells (C) . *P <0.05 vs. “Control” treatment in “Gαi1/3-shRNA” EoL-1 cells (D) . #P <0.05 vs. GM-CSF and IL-5 treatment in “Gαi1/3-shRNA” EoL-1 cells (D) .
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    ( A ) <t>CD125</t> but not CD69 ( B ) or CD193 ( C ) was increased in circulating eosinophils from ER patients versus healthy donors. * p < 0.05. Each dot represents a unique donor. N = 7 ER; N = 10 HD.
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    ( A ) <t>CD125</t> but not CD69 ( B ) or CD193 ( C ) was increased in circulating eosinophils from ER patients versus healthy donors. * p < 0.05. Each dot represents a unique donor. N = 7 ER; N = 10 HD.
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    Image Search Results


    IL-5 induced Gαi1/3 association with activated IL-5 receptor and Gab1. WT and Gαi1/3 DKO MEFs were treated with IL-5 (25 ng/mL) for 5 min, the association between IL-5Rα, Gab1, and Gαi1/3 was tested by co-ip assay (A) . EoL-1 cells were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 5 min, the association between IL-5Rα, Gab1, and Gαi1/3 was tested by co-ip (B) , and processed for confocal microscopy to detect the presence of Gαi1, Gαi3, and IL-5Rα (C) . Bar = 1 μm.

    Journal: Frontiers in Immunology

    Article Title: Gαi1/3 signaling mediates IL-5-induced eosinophil activation and type 2 inflammation in eosinophilic chronic rhinosinusitis

    doi: 10.3389/fimmu.2024.1460104

    Figure Lengend Snippet: IL-5 induced Gαi1/3 association with activated IL-5 receptor and Gab1. WT and Gαi1/3 DKO MEFs were treated with IL-5 (25 ng/mL) for 5 min, the association between IL-5Rα, Gab1, and Gαi1/3 was tested by co-ip assay (A) . EoL-1 cells were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 5 min, the association between IL-5Rα, Gab1, and Gαi1/3 was tested by co-ip (B) , and processed for confocal microscopy to detect the presence of Gαi1, Gαi3, and IL-5Rα (C) . Bar = 1 μm.

    Article Snippet: Antibodies against Gαi1 (sc-13533), Gαi2 (sc-13534), Gαi3 (sc-365422), Gab1 (sc-133191), Akt (sc-81434), Erk1/2 (sc-514302), S6K (sc-8418), p-Gab1 (AP0256), p-Akt s473 (sc-101629), p-Erk1/2 (sc-136521), p-S6K (sc-8416), STAT5 (sc-74442), p-STAT5 (AP-0887), IL-5Rα (bs-2601R-100ul), IgE (ab75673), and tryptase (ab2378) were purchased from Bioss (Woburn, MA, USA), ABcolnal (Wuhan, China), Abcam (Cambridge, MA, USA), and Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Co-Immunoprecipitation Assay, Confocal Microscopy

    Gαi1/3 knockdown inhibits STAT5 activation, differentiation and growth by IL-5 in EoL-1 cells. Stable EoL-1 cells, expressing the scramble control shRNA (“C-shRNA”), Gαi1 shRNA and Gαi3 shRNA, were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 24 hours, and were tested by Western blotting of listed proteins (A, E) . Relative expression of listed genes (24 hours after GM-CSF and IL-5 treatment) was shown (B, C) ; In each experiment, n=3 (three replicated wells). Cells were further cultured for the times indicated, cell proliferation were tested (F, G) , with cell viability tested by CCK-8 assay (D) . Bar = 100 μm. Blotting quantification was performed from five replicate blot data, data of all repeated experiments were pulled together to calculate mean ± SD. *P <0.05 (B, E, G) . *P <0.05 vs. “Control” treatment in “C-shRNA” EoL-1 cells (C) . # P <0.05 vs. GM-CSF and IL-5 treatment in “C-shRNA” EoL-1 cells (C) . *P <0.05 vs. “Control” treatment in “Gαi1/3-shRNA” EoL-1 cells (D) . #P <0.05 vs. GM-CSF and IL-5 treatment in “Gαi1/3-shRNA” EoL-1 cells (D) .

    Journal: Frontiers in Immunology

    Article Title: Gαi1/3 signaling mediates IL-5-induced eosinophil activation and type 2 inflammation in eosinophilic chronic rhinosinusitis

    doi: 10.3389/fimmu.2024.1460104

    Figure Lengend Snippet: Gαi1/3 knockdown inhibits STAT5 activation, differentiation and growth by IL-5 in EoL-1 cells. Stable EoL-1 cells, expressing the scramble control shRNA (“C-shRNA”), Gαi1 shRNA and Gαi3 shRNA, were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 24 hours, and were tested by Western blotting of listed proteins (A, E) . Relative expression of listed genes (24 hours after GM-CSF and IL-5 treatment) was shown (B, C) ; In each experiment, n=3 (three replicated wells). Cells were further cultured for the times indicated, cell proliferation were tested (F, G) , with cell viability tested by CCK-8 assay (D) . Bar = 100 μm. Blotting quantification was performed from five replicate blot data, data of all repeated experiments were pulled together to calculate mean ± SD. *P <0.05 (B, E, G) . *P <0.05 vs. “Control” treatment in “C-shRNA” EoL-1 cells (C) . # P <0.05 vs. GM-CSF and IL-5 treatment in “C-shRNA” EoL-1 cells (C) . *P <0.05 vs. “Control” treatment in “Gαi1/3-shRNA” EoL-1 cells (D) . #P <0.05 vs. GM-CSF and IL-5 treatment in “Gαi1/3-shRNA” EoL-1 cells (D) .

    Article Snippet: Antibodies against Gαi1 (sc-13533), Gαi2 (sc-13534), Gαi3 (sc-365422), Gab1 (sc-133191), Akt (sc-81434), Erk1/2 (sc-514302), S6K (sc-8418), p-Gab1 (AP0256), p-Akt s473 (sc-101629), p-Erk1/2 (sc-136521), p-S6K (sc-8416), STAT5 (sc-74442), p-STAT5 (AP-0887), IL-5Rα (bs-2601R-100ul), IgE (ab75673), and tryptase (ab2378) were purchased from Bioss (Woburn, MA, USA), ABcolnal (Wuhan, China), Abcam (Cambridge, MA, USA), and Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Knockdown, Activation Assay, Expressing, Control, shRNA, Western Blot, Cell Culture, CCK-8 Assay

    ( A ) CD125 but not CD69 ( B ) or CD193 ( C ) was increased in circulating eosinophils from ER patients versus healthy donors. * p < 0.05. Each dot represents a unique donor. N = 7 ER; N = 10 HD.

    Journal: Cells

    Article Title: Granular Insights: Neutrophil Predominance and Elastase Release in Severe Asthma Exacerbations in a Pediatric Cohort

    doi: 10.3390/cells13060533

    Figure Lengend Snippet: ( A ) CD125 but not CD69 ( B ) or CD193 ( C ) was increased in circulating eosinophils from ER patients versus healthy donors. * p < 0.05. Each dot represents a unique donor. N = 7 ER; N = 10 HD.

    Article Snippet: CD11b (BD 555386), L-selectin (BD 555542), and ICAM-1 (BD 555510) were measured in neutrophils, and CD125 (IL5Ra) (BD 555902), CD69 (BD 560738), and CD193 (BD 564189) were measured in eosinophils.

    Techniques: