anti cd11b coated microbeads  (Miltenyi Biotec)

Journal: Biology Direct

Article Title: The role of SH3RF2 in lung squamous cell carcinoma and M2 polarization: insights into LZTS2 ubiquitination

doi: 10.1186/s13062-025-00677-0

Figure Lengend Snippet: ( A & B ) SKMES1 cells with SH3RF2 overexpression or knockdown were injected subcutaneously into the right armpit of Balb/c nude mice. Tumor size was measured every 3 days. Tumors were removed and weighed after 33 days. ( C & D ) The protein level of CCL2 in tumor determined by ELISA. ( E ) Detection of SH3RF2 expression in tumor tissues by immunohistochemistry, bar = 50 μm. ( F ) Immunohistochemistry for Ki-67 in tumor tissues, bar = 50 μm. ( G ) The proportion of CD11b + F4/80 + CD206 + cells in tumor. NC sh : negative control shRNA, SR sh−1 : SH3RF2 shRNA-1, SR sh−2 : SH3RF2 shRNA-2, EV: empty vector, SR oe : SH3RF3 overexpression. **: p < 0.01, ***: p < 0.001

Article Snippet: Resuspended cells were incubated with CD11b antibody-coated magnetic beads (130-097-142, Miltenyi Biotec, Germany) at 4°C for 15 min. CD11b + cells were resuspended with PBS and incubated with mouse F4/80 (F21480A02, Lianke Bio, China) and CD206 antibody (53-2061-80, Thermo Fisher Scientific, USA) for 30 min at 4°C in the dark, and detected by a flow cytometer.

Techniques: Over Expression, Knockdown, Injection, Enzyme-linked Immunosorbent Assay, Expressing, Immunohistochemistry, Negative Control, shRNA, Plasmid Preparation