ccl2  (Kingfisher Biotech)


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    Name:
    Bovine CCL2 MCP 1 Do It Yourself ELISA
    Description:

    Catalog Number:
    DIY0659B-003
    Price:
    700.0
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    1 Set
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    Kingfisher Biotech ccl2
    Changes in levels of mRNAs for porcine CC ligands following infection of pigs with different ASFV isolates. Changes in mRNA for chemokine and chemokine receptor genes in porcine blood cells were estimated by real time RT PCR and are shown for samples from experiment 2. Pigs were either uninfected or infected with ASFV isolates, OURT88/3 or Benin97/1. Samples were taken at 0 3, 7, 14 or 20 dpi and RNA was extracted. mRNA levels were measured by real time RT-PCR using SYBR Green (Sigma). Changes are shown as Log 2 fold changes (y-axis) compared to 2 time points pre-infection to give the calibrator sample. No amplification was detected for <t>CCL2</t> mRNA in calibrator samples so data shown is the Ct value. Data from real time RT PCR for (A) CCL2, (B) CCL3L, (C) CCL4, (D) CCL5, (E) CCR1, (F) CCR5, (G) CXCL10, are shown. The dpi is shown on the x axis and the different isolates are indicated on the right of each panel. The mean for each group ± SEM is shown, n = 3-6 pigs.

    https://www.bioz.com/result/ccl2/product/Kingfisher Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ccl2 - by Bioz Stars, 2021-10
    93/100 stars

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    1) Product Images from "Increase in chemokines CXCL10 and CCL2 in blood from pigs infected with high compared to low virulence African swine fever virus isolates"

    Article Title: Increase in chemokines CXCL10 and CCL2 in blood from pigs infected with high compared to low virulence African swine fever virus isolates

    Journal: Veterinary Research

    doi: 10.1186/1297-9716-44-87

    Changes in levels of mRNAs for porcine CC ligands following infection of pigs with different ASFV isolates. Changes in mRNA for chemokine and chemokine receptor genes in porcine blood cells were estimated by real time RT PCR and are shown for samples from experiment 2. Pigs were either uninfected or infected with ASFV isolates, OURT88/3 or Benin97/1. Samples were taken at 0 3, 7, 14 or 20 dpi and RNA was extracted. mRNA levels were measured by real time RT-PCR using SYBR Green (Sigma). Changes are shown as Log 2 fold changes (y-axis) compared to 2 time points pre-infection to give the calibrator sample. No amplification was detected for CCL2 mRNA in calibrator samples so data shown is the Ct value. Data from real time RT PCR for (A) CCL2, (B) CCL3L, (C) CCL4, (D) CCL5, (E) CCR1, (F) CCR5, (G) CXCL10, are shown. The dpi is shown on the x axis and the different isolates are indicated on the right of each panel. The mean for each group ± SEM is shown, n = 3-6 pigs.
    Figure Legend Snippet: Changes in levels of mRNAs for porcine CC ligands following infection of pigs with different ASFV isolates. Changes in mRNA for chemokine and chemokine receptor genes in porcine blood cells were estimated by real time RT PCR and are shown for samples from experiment 2. Pigs were either uninfected or infected with ASFV isolates, OURT88/3 or Benin97/1. Samples were taken at 0 3, 7, 14 or 20 dpi and RNA was extracted. mRNA levels were measured by real time RT-PCR using SYBR Green (Sigma). Changes are shown as Log 2 fold changes (y-axis) compared to 2 time points pre-infection to give the calibrator sample. No amplification was detected for CCL2 mRNA in calibrator samples so data shown is the Ct value. Data from real time RT PCR for (A) CCL2, (B) CCL3L, (C) CCL4, (D) CCL5, (E) CCR1, (F) CCR5, (G) CXCL10, are shown. The dpi is shown on the x axis and the different isolates are indicated on the right of each panel. The mean for each group ± SEM is shown, n = 3-6 pigs.

    Techniques Used: Infection, Quantitative RT-PCR, SYBR Green Assay, Amplification

    Amount of CCL2, IFN γ and CXCL8 in plasma samples from ASFV infected and uninfected pigs. Pigs were infected with high virulence ASFV isolates Benin 97/1, Uganda 1965 or low virulence isolate OURT88/3 and plasma samples were collected at different days post-infection as indicated on the x-axis. The amount of CCL2 ( Panels A and B) , CXCL8 ( Panels D and E) and IFN γ ( Panels E and F) present was estimated by ELISA assay. Values for samples from pigs infected with different isolates or uninfected pigs are indicated by different coloured bars. Error bars indicate SEMs. The results from experiment 1 are shown in panels A , C , E and from experiment 2 in B , D , F .
    Figure Legend Snippet: Amount of CCL2, IFN γ and CXCL8 in plasma samples from ASFV infected and uninfected pigs. Pigs were infected with high virulence ASFV isolates Benin 97/1, Uganda 1965 or low virulence isolate OURT88/3 and plasma samples were collected at different days post-infection as indicated on the x-axis. The amount of CCL2 ( Panels A and B) , CXCL8 ( Panels D and E) and IFN γ ( Panels E and F) present was estimated by ELISA assay. Values for samples from pigs infected with different isolates or uninfected pigs are indicated by different coloured bars. Error bars indicate SEMs. The results from experiment 1 are shown in panels A , C , E and from experiment 2 in B , D , F .

    Techniques Used: Infection, Enzyme-linked Immunosorbent Assay

    Changes in levels of mRNAs for porcine CC ligands following infection of pigs with different ASFV isolates. Changes in mRNA for chemokine and chemokine receptor genes in porcine blood cells were estimated by real time RT PCR and are shown for samples from experiment 1. Pigs were either uninfected or infected with ASFV isolates, OURT88/3 or Benin97/1. Samples were taken 3, 5, 12 or 26 dpi and RNA was extracted. mRNA levels were measured by real time RT-PCR using SYBR Green (Sigma). Changes are shown as Log 2 fold changes on the y axis compared to 1 time point pre-infection to give the calibrator sample. Data from real time RT PCR for (A) CCL2, (B) CCL3L, (C) CCL4, (D) CCL5, (E) CCR1, (F) CCR5, (G) CXCL10 are shown. The dpi samples were collected is shown on the x axis and the different isolates are indicated on the right of each panel. The mean for each group ± SEM is shown, n = 3-6 pigs.
    Figure Legend Snippet: Changes in levels of mRNAs for porcine CC ligands following infection of pigs with different ASFV isolates. Changes in mRNA for chemokine and chemokine receptor genes in porcine blood cells were estimated by real time RT PCR and are shown for samples from experiment 1. Pigs were either uninfected or infected with ASFV isolates, OURT88/3 or Benin97/1. Samples were taken 3, 5, 12 or 26 dpi and RNA was extracted. mRNA levels were measured by real time RT-PCR using SYBR Green (Sigma). Changes are shown as Log 2 fold changes on the y axis compared to 1 time point pre-infection to give the calibrator sample. Data from real time RT PCR for (A) CCL2, (B) CCL3L, (C) CCL4, (D) CCL5, (E) CCR1, (F) CCR5, (G) CXCL10 are shown. The dpi samples were collected is shown on the x axis and the different isolates are indicated on the right of each panel. The mean for each group ± SEM is shown, n = 3-6 pigs.

    Techniques Used: Infection, Quantitative RT-PCR, SYBR Green Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Increase in chemokines CXCL10 and CCL2 in blood from pigs infected with high compared to low virulence African swine fever virus isolates
    Article Snippet: .. ELISA assays ELISA assays to detect Interferon gamma, CXCL8 and CCL2 in defibrinated plasma samples were carried out according to the manufacturer’s recommendations (Kingfisher Biotech. ..

    Article Title: Effect of the Ketone Body Beta-Hydroxybutyrate on the Innate Defense Capability of Primary Bovine Mammary Epithelial Cells
    Article Snippet: .. For the determination of the CCL2 amount in cell culture supernatants, the bovine CCL2 VetSet™ ELISA Development Kit (Kingfisher Biotech, St Paul, USA) was used. ..

    Article Title: Pathogenesis of percutaneous infection of goats with Burkholderia pseudomallei: clinical, pathologic, and immunological responses in chronic melioidosis
    Article Snippet: .. Analysis was performed using commercially available enzyme-linked immunosorbent assay (ELISA) kits for bovine CCL2 (VS0083B; Kingfisher Biotech, Inc., St. Paul, MN, USA), bovine IFNγ (VS0257B; Kingfisher Biotech, Inc.), bovine IL-10 (BV1495; TSZ ELISA, Framingham, MA, USA) and TGF-β1 (MB100B; R & D Systems, Minneapolis, MN, USA). ..

    Article Title: Differential chemokine and cytokine production by neonatal bovine ?? T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection
    Article Snippet: .. The Bovine monocyte chemoattractant protein 1 (MCP-1; CCL2) VetSet ELISA Development kit was purchased from Kingfisher Biotech, Inc. (St Paul, MN). ..

    Article Title: Evaluation of Fat Accumulation and Adipokine Production during the Long-Term Adipogenic Differentiation of Porcine Intramuscular Preadipocytes and Study of the Influence of Immunobiotics
    Article Snippet: .. ELISA AssayThe protein concentration of IL-6, IL-8, IL-10, CCL2, leptin and adiponectin in the cell-free supernatant (100 μL) of adipocytes culture were measured by using commercially available ELISA kits: porcine IL-6, IL-8, and IL-10 ELISA kits (R & D Systems, Minneapolis, MN, USA); porcine CCL2 ELISA kit (Kingfisher Biotech, Inc. ST. Paul, MN, USA); pig leptin ELISA Kit (Wuhan Huamei Biotech Co., Ltd., Wuhan, China); and pig adiponectin ELISA Kit (Wuhan Huamei Biotech Co., Ltd., Wuhan, China). ..

    Article Title: Effect of the Ketone Body Beta-Hydroxybutyrate on the Innate Defense Capability of Primary Bovine Mammary Epithelial Cells
    Article Snippet: .. The same trend could be seen when the RT-qPCR data of CCL2 ( , ) was compared to the protein data obtained by the bovine CCL2 VetSet™ ELISA Development Kit. ..

    Article Title: Maternal Intravenous Administration of Azithromycin Results in Significant Fetal Uptake in a Sheep Model of Second Trimester Pregnancy
    Article Snippet: .. AF MCP-1 concentrations, a marker of intrauterine inflammation in sheep, were measured using an MCP-1 ELISA VetSet kit (VS0083B-002; Kingfisher Biotech, Inc., Saint Paul, MN), with washing performed on a Biosan plate washer (Intelliwasher 3D-IW8; Biosan, Riga, Latvia). ..

    Cell Culture:

    Article Title: Effect of the Ketone Body Beta-Hydroxybutyrate on the Innate Defense Capability of Primary Bovine Mammary Epithelial Cells
    Article Snippet: .. For the determination of the CCL2 amount in cell culture supernatants, the bovine CCL2 VetSet™ ELISA Development Kit (Kingfisher Biotech, St Paul, USA) was used. ..

    Protein Concentration:

    Article Title: Evaluation of Fat Accumulation and Adipokine Production during the Long-Term Adipogenic Differentiation of Porcine Intramuscular Preadipocytes and Study of the Influence of Immunobiotics
    Article Snippet: .. ELISA AssayThe protein concentration of IL-6, IL-8, IL-10, CCL2, leptin and adiponectin in the cell-free supernatant (100 μL) of adipocytes culture were measured by using commercially available ELISA kits: porcine IL-6, IL-8, and IL-10 ELISA kits (R & D Systems, Minneapolis, MN, USA); porcine CCL2 ELISA kit (Kingfisher Biotech, Inc. ST. Paul, MN, USA); pig leptin ELISA Kit (Wuhan Huamei Biotech Co., Ltd., Wuhan, China); and pig adiponectin ELISA Kit (Wuhan Huamei Biotech Co., Ltd., Wuhan, China). ..

    Quantitative RT-PCR:

    Article Title: Effect of the Ketone Body Beta-Hydroxybutyrate on the Innate Defense Capability of Primary Bovine Mammary Epithelial Cells
    Article Snippet: .. The same trend could be seen when the RT-qPCR data of CCL2 ( , ) was compared to the protein data obtained by the bovine CCL2 VetSet™ ELISA Development Kit. ..

    Marker:

    Article Title: Maternal Intravenous Administration of Azithromycin Results in Significant Fetal Uptake in a Sheep Model of Second Trimester Pregnancy
    Article Snippet: .. AF MCP-1 concentrations, a marker of intrauterine inflammation in sheep, were measured using an MCP-1 ELISA VetSet kit (VS0083B-002; Kingfisher Biotech, Inc., Saint Paul, MN), with washing performed on a Biosan plate washer (Intelliwasher 3D-IW8; Biosan, Riga, Latvia). ..

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    Kingfisher Biotech porcine ccl2 elisa kit
    Long-term adipogenic differentiation mediated expression dynamics of adipokines in the adipocytes without any exogenous inflammatory triggers. The mRNA expression ( A ) and protein concentration ( B ) of <t>CCL2,</t> IL-6, IL-8 and IL-10 in adipocytes over the 20 days of culture as measured by RT-qPCR and <t>ELISA,</t> respectively. Data shown are the mean ± SD of three independent experiments performed in triplicates. The asterisks: (*) and (**) indicated statistical differences when compared with day 0 at the significance levels of p
    Porcine Ccl2 Elisa Kit, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/porcine ccl2 elisa kit/product/Kingfisher Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    porcine ccl2 elisa kit - by Bioz Stars, 2021-10
    93/100 stars
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    Long-term adipogenic differentiation mediated expression dynamics of adipokines in the adipocytes without any exogenous inflammatory triggers. The mRNA expression ( A ) and protein concentration ( B ) of CCL2, IL-6, IL-8 and IL-10 in adipocytes over the 20 days of culture as measured by RT-qPCR and ELISA, respectively. Data shown are the mean ± SD of three independent experiments performed in triplicates. The asterisks: (*) and (**) indicated statistical differences when compared with day 0 at the significance levels of p

    Journal: Cells

    Article Title: Evaluation of Fat Accumulation and Adipokine Production during the Long-Term Adipogenic Differentiation of Porcine Intramuscular Preadipocytes and Study of the Influence of Immunobiotics

    doi: 10.3390/cells9071715

    Figure Lengend Snippet: Long-term adipogenic differentiation mediated expression dynamics of adipokines in the adipocytes without any exogenous inflammatory triggers. The mRNA expression ( A ) and protein concentration ( B ) of CCL2, IL-6, IL-8 and IL-10 in adipocytes over the 20 days of culture as measured by RT-qPCR and ELISA, respectively. Data shown are the mean ± SD of three independent experiments performed in triplicates. The asterisks: (*) and (**) indicated statistical differences when compared with day 0 at the significance levels of p

    Article Snippet: ELISA AssayThe protein concentration of IL-6, IL-8, IL-10, CCL2, leptin and adiponectin in the cell-free supernatant (100 μL) of adipocytes culture were measured by using commercially available ELISA kits: porcine IL-6, IL-8, and IL-10 ELISA kits (R & D Systems, Minneapolis, MN, USA); porcine CCL2 ELISA kit (Kingfisher Biotech, Inc. ST. Paul, MN, USA); pig leptin ELISA Kit (Wuhan Huamei Biotech Co., Ltd., Wuhan, China); and pig adiponectin ELISA Kit (Wuhan Huamei Biotech Co., Ltd., Wuhan, China).

    Techniques: Expressing, Protein Concentration, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    Immunobiotic mediated regulation of fat accumulation and inflammatory responses in an immune cell-adipocyte co-culture model. A total of 1 × 10 6 immune cells/well of a 6-well plate were cultured on adipocytes 4-days differentiated. Bacterial samples (5 × 10 8 cells/well) were also added and cultured for 2 or 4 days. Adipocyte cells were stained with Oil red O stained and fat accumulation in the adipocytes was quantitatively analyzed by image J software. The ELISA-based concentration of CCL2 protein in supernatant of adipocyte-culture stimulated by immune cells and lactic acid bacteria (LAB). All data shown are the mean ± SD of three independent experiments performed in triplicate. The asterisks: ‘*’ and ‘**’ indicated statistical differences either between bars of panel-a and control or between bars of panel-b and IMU, with significant levels of p

    Journal: Cells

    Article Title: Evaluation of Fat Accumulation and Adipokine Production during the Long-Term Adipogenic Differentiation of Porcine Intramuscular Preadipocytes and Study of the Influence of Immunobiotics

    doi: 10.3390/cells9071715

    Figure Lengend Snippet: Immunobiotic mediated regulation of fat accumulation and inflammatory responses in an immune cell-adipocyte co-culture model. A total of 1 × 10 6 immune cells/well of a 6-well plate were cultured on adipocytes 4-days differentiated. Bacterial samples (5 × 10 8 cells/well) were also added and cultured for 2 or 4 days. Adipocyte cells were stained with Oil red O stained and fat accumulation in the adipocytes was quantitatively analyzed by image J software. The ELISA-based concentration of CCL2 protein in supernatant of adipocyte-culture stimulated by immune cells and lactic acid bacteria (LAB). All data shown are the mean ± SD of three independent experiments performed in triplicate. The asterisks: ‘*’ and ‘**’ indicated statistical differences either between bars of panel-a and control or between bars of panel-b and IMU, with significant levels of p

    Article Snippet: ELISA AssayThe protein concentration of IL-6, IL-8, IL-10, CCL2, leptin and adiponectin in the cell-free supernatant (100 μL) of adipocytes culture were measured by using commercially available ELISA kits: porcine IL-6, IL-8, and IL-10 ELISA kits (R & D Systems, Minneapolis, MN, USA); porcine CCL2 ELISA kit (Kingfisher Biotech, Inc. ST. Paul, MN, USA); pig leptin ELISA Kit (Wuhan Huamei Biotech Co., Ltd., Wuhan, China); and pig adiponectin ELISA Kit (Wuhan Huamei Biotech Co., Ltd., Wuhan, China).

    Techniques: Co-Culture Assay, Cell Culture, Staining, Software, Enzyme-linked Immunosorbent Assay, Concentration Assay

    Comparison of the RT-qPCR data of LF and CCL2 with the LF and CCL2 content in pbMEC cell culture supernatants. The fold changes of the LF gene expression (A) indicated a significant down-regulation of LF gene expression when pbMEC where co-stimulated with E . coli and 3 mM BHBA. The same effect could be detected within the competitive LF ELISA (B) of pbMEC cell culture supernatants. The amount of secreted LF decreased significantly in case of the co-stimulation. The distinct and significant down-regulation in CCL2 gene expression (C) could also be confirmed by the results of the bovine CCL2 VetSet ™ ELISA Kit (D). The CCL2 gene expression and the amount of secreted protein decreased distinctly as well as significantly within the co-stimulatory group. Significant changes: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, distinct changes: + 0.1 ≤ p ≤ 0.05.

    Journal: PLoS ONE

    Article Title: Effect of the Ketone Body Beta-Hydroxybutyrate on the Innate Defense Capability of Primary Bovine Mammary Epithelial Cells

    doi: 10.1371/journal.pone.0157774

    Figure Lengend Snippet: Comparison of the RT-qPCR data of LF and CCL2 with the LF and CCL2 content in pbMEC cell culture supernatants. The fold changes of the LF gene expression (A) indicated a significant down-regulation of LF gene expression when pbMEC where co-stimulated with E . coli and 3 mM BHBA. The same effect could be detected within the competitive LF ELISA (B) of pbMEC cell culture supernatants. The amount of secreted LF decreased significantly in case of the co-stimulation. The distinct and significant down-regulation in CCL2 gene expression (C) could also be confirmed by the results of the bovine CCL2 VetSet ™ ELISA Kit (D). The CCL2 gene expression and the amount of secreted protein decreased distinctly as well as significantly within the co-stimulatory group. Significant changes: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, distinct changes: + 0.1 ≤ p ≤ 0.05.

    Article Snippet: The same trend could be seen when the RT-qPCR data of CCL2 ( , ) was compared to the protein data obtained by the bovine CCL2 VetSet™ ELISA Development Kit.

    Techniques: Quantitative RT-PCR, Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay

    Uterine flush cell counts and immunotyping of luminal cell populations of sows 24 h after breeding with semen only (SO) or semen containing a triple adjuvant combination (STA) in addition to PBMC immunotyping before or 24 h after breeding. Flushed cells were counted by coulter counter (A) . Luminal CCL2 was quantified by sandwich ELISA (B) and significant differences between treatments were determined by Mann Whitney test. Immunotyped cells in the uterine flush were stained with CD3, CD4, CD8α, γδ T cells, CD172, MHCII, SWC9, and CD16 (C) . PBMCs were isolated from blood and stained for CD3, CD4, CD8α, γδ T cells, CD21, CD172, and CD14 (D) . Stained cells were analyzed on a FACScalibur and significant differences between treatments determined by Mann Whitney test. Each circle or square represents a unique biological replicate and the line represents mean data. * p > 0.05.

    Journal: Frontiers in Immunology

    Article Title: Assessment of Immunological Response and Impacts on Fertility Following Intrauterine Vaccination Delivered to Swine in an Artificial Insemination Dose

    doi: 10.3389/fimmu.2020.01015

    Figure Lengend Snippet: Uterine flush cell counts and immunotyping of luminal cell populations of sows 24 h after breeding with semen only (SO) or semen containing a triple adjuvant combination (STA) in addition to PBMC immunotyping before or 24 h after breeding. Flushed cells were counted by coulter counter (A) . Luminal CCL2 was quantified by sandwich ELISA (B) and significant differences between treatments were determined by Mann Whitney test. Immunotyped cells in the uterine flush were stained with CD3, CD4, CD8α, γδ T cells, CD172, MHCII, SWC9, and CD16 (C) . PBMCs were isolated from blood and stained for CD3, CD4, CD8α, γδ T cells, CD21, CD172, and CD14 (D) . Stained cells were analyzed on a FACScalibur and significant differences between treatments determined by Mann Whitney test. Each circle or square represents a unique biological replicate and the line represents mean data. * p > 0.05.

    Article Snippet: CCL2 ELISAUterine horn luminal CCL2 was quantified by sandwich ELISA against porcine CCL2 (Kingfisher Biotech) following manufacturer's instructions.

    Techniques: Sandwich ELISA, MANN-WHITNEY, Staining, Isolation

    Cytokine production in goats infected with B. pseudomallei . Responses of (a) TGF-β1 (log pg/ml), (b) IFNγ (log ng/ml) and (c) CCL2 (log ng/ml) in aerosol ( ) and percutaneous ( ) groups. Significant elevations were only present for

    Journal: International Journal of Experimental Pathology

    Article Title: Pathogenesis of percutaneous infection of goats with Burkholderia pseudomallei: clinical, pathologic, and immunological responses in chronic melioidosis

    doi: 10.1111/iep.12068

    Figure Lengend Snippet: Cytokine production in goats infected with B. pseudomallei . Responses of (a) TGF-β1 (log pg/ml), (b) IFNγ (log ng/ml) and (c) CCL2 (log ng/ml) in aerosol ( ) and percutaneous ( ) groups. Significant elevations were only present for

    Article Snippet: Analysis was performed using commercially available enzyme-linked immunosorbent assay (ELISA) kits for bovine CCL2 (VS0083B; Kingfisher Biotech, Inc., St. Paul, MN, USA), bovine IFNγ (VS0257B; Kingfisher Biotech, Inc.), bovine IL-10 (BV1495; TSZ ELISA, Framingham, MA, USA) and TGF-β1 (MB100B; R & D Systems, Minneapolis, MN, USA).

    Techniques: Infection