Journal: Materials Today Bio

Article Title: Development of polypeptide scaffold for anti-endometrial invasion and ordered myometrial recovery in uterine diverticulum

doi: 10.1016/j.mtbio.2025.102651

Figure Lengend Snippet: Preparation and characterization of BSBs. (a) A cartoon diagram illustrates the manufacturing process of BSBs and immunohistochemistry (IHC) images of the natural anatomical structure of the endometrium. (b) The process of injecting agarose-SF solution into the mold and actual pictures of the BSBs. (c) Photographs show a comparison of BSBs before and after freeze-drying. (d) The curing times of SF solutions containing 1 %, 2 %, and 3 % agarose. (e) The CCK8 assay measured the differences in the proliferation ability of uSMCs in response to various concentrations of agarose SF solutions. (f) The wound healing assay assessed the impact of different concentrations of agarose SF solutions on the migration ability of uSMCs. (g) The transwell assay evaluated the influence of various concentrations of agarose SF solutions on the invasion ability of uSMCs. (h) Conceptual diagrams and SEM images depicted random and directional biomaterial scaffolds. (i) Angle analysis results of random and oriented biomaterial scaffolds were presented. (j) FTIR analysis results for SF scaffolds, both before and after the addition of agarose. (k) XRD analysis of SF and 2 % agarose-SF. (l) Compression test data were reported for pure SF scaffolds, random BSBs, and oriented BSBs. (m) Statistical analysis results of the compression modulus, maximum stress, and toughness of pure SF scaffolds, random BSBs, and oriented BSBs were included. (n) Degradation of the 2 % agarose-SF bioscaffold at PBS and protease XIV. All data are shown as the mean ± SD of three independent experiments (∗∗∗ P < 0.001,∗∗∗∗ P < 0.0001). Scale bars indicate 200 μm a), 10 mm b), 1 mm c), 500 μm f,g), and 20 μm h).

Article Snippet: CCK8 was purchased from MCE (HY-K0301).

Techniques: Immunohistochemistry, Comparison, CCK-8 Assay, Wound Healing Assay, Migration, Transwell Assay

Journal: Materials Today Bio

Article Title: Development of polypeptide scaffold for anti-endometrial invasion and ordered myometrial recovery in uterine diverticulum

doi: 10.1016/j.mtbio.2025.102651

Figure Lengend Snippet: Organized cells growth on BSBs. (a) A cartoon diagram illustrated the culture of uSMCs and HUVECs in BSBs. (b) SEM images displayed the culture of uSMCs and HUVECs in directional-BSBs. (c) Immunofluorescence images showed random and directional-BSBs with cultured uSMCs and HUVECs. (d) Cross-sectional and longitudinal immunofluorescence images depicted the cultured uSMCs and HUVECs in directional-BSBs. (e) Confocal images showing single pore regions of BSBs seeded with uSMCs and HUVECs, illustrating the depth of cellular infiltration in directional BSBs. (f) CCK8 assay assessing the viability and proliferation of uSMCs and HUVECs cultured in BSBs extract solutions over 7 days. (g) Representative IF images and semi-quantitative analysis of CD140b and SUSD2 expression in DAFs co-cultured with or without BSBs for 7 days. (h) qRT-PCR analysis of OCT4 and NANOG expression in DAFs co-cultured with or without BSBs for 7 days. All data are shown as the mean ± SD of three independent experiments (∗∗ P < 0.01, ∗∗∗ P < 0.001,∗∗∗∗ P < 0.0001). Scale bars, 1 mm b, i), 10 μm in b, ii and iii), 250 μm in c, d), 500 μm in e), and 20 μm in g).

Article Snippet: CCK8 was purchased from MCE (HY-K0301).

Techniques: Immunofluorescence, Cell Culture, CCK-8 Assay, Expressing, Quantitative RT-PCR

Journal: Materials Today Bio

Article Title: Development of polypeptide scaffold for anti-endometrial invasion and ordered myometrial recovery in uterine diverticulum

doi: 10.1016/j.mtbio.2025.102651

Figure Lengend Snippet: Functional characterization of exosomes derived from M-ESCs. (a) TEM observation examined the morphology of exosomes derived from M-ESCs. (b) Size distribution of exosomes observed in TEM images (n = 200). (c) DLS measured the average particle size of exosomes. (d) WB detection of the marker proteins CD63 and TSG101 in exosomes (n = 3). (e) CCK-8 method assay evaluating the proliferative effects of exosomes derived from M-ESCs on DAFs and HUVECs over 0–48 h (n = 6). (f) CCK-8 assay examining the dose-dependent effects of M-ESC–derived exosomes (0–60 μg/mL) on DAFs and HUVECs proliferation (n = 6). (g) DNA content analysis quantifying uSMCs proliferation within BSBs co-cultured with exosomes d for 1–6 days (n = 6). (h) DNA content analysis examined the proliferation of HUVECs co-cultured with M-ESC–derived exosomes within BSBs over 1–6 days (n = 6). All data are shown as the mean ± SD of three independent experiments (∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). Scale bars: 200 nm in a).

Article Snippet: CCK8 was purchased from MCE (HY-K0301).

Techniques: Functional Assay, Derivative Assay, Marker, CCK-8 Assay, Cell Culture