Journal: Frontiers in Immunology

Article Title: Microenvironmental regulation of T-cells in pulmonary hypertension

doi: 10.3389/fimmu.2023.1223122

Figure Lengend Snippet: Used antibodies for immunophenotyping.

Article Snippet: Mouse anti-bovine IL10 , CC320 , Thermo Fisher , 1:100 , IgG1 , intra , PerCP.

Techniques: Staining, Conjugation Assay

Journal: Veterinary Research

Article Title: Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products

doi: 10.1186/1297-9716-44-70

Figure Lengend Snippet: Gene expression in helminth-free PBMC cultures following stimulation with Con A ± Tci - L4 - ES. PBMC from helminth-naïve lambs cultured for 72 h with PBS alone (PBS), 5 μg/mL Con A alone (ConA + PBS), 5 μg/mL Con A + 30 μg/mL Tci -L4-ES (ConA + ES) or 5 μg/mL Con A + 30 μg/mL heat-inactivated Tci -L4-ES (ConA + HiES). Relative gene expression of IL - 10 (A) , TGF -β 1 (B) , IL - 4 (C) and IFN -γ (D) was determined by quantitative RT-PCR. Data represents mean ± SEM from three replicate cultures from three helminth-free lambs. ** P < 0.01, *** P < 0.001 (one way ANOVA followed by the Tukey post hoc test for pairwise comparison of means).

Article Snippet: To determine the role of interleukin-10 (IL-10) PBMC were cultured in the presence of 5 μg/mL anti-ovine IL-10 monoclonal antibody (mAb) CC320 (AbDserotec, Kidlington, UK) or 5 μg/mL mouse IgG1 isotype control (eBioscience).

Techniques: Expressing, Cell Culture, Quantitative RT-PCR

Journal: Veterinary Research

Article Title: Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products

doi: 10.1186/1297-9716-44-70

Figure Lengend Snippet: The effect of IL - 10 neutralization on Tci - L4 - ES mediated lymphocyte suppression. PBMC from helminth-naïve lambs were cultured with ConA + 15 μg/mL Tci -L4-ES in the presence or absence of 5 μg/mL IL-10 neutralizing mAb CC320 (ConA+ES+αIL10) or 5 μg/mL IgG1 isotype control mAb (ConA+ES+isotype). Proliferation was assessed after 72 h by measurement of [3H] thymidine incorporation. Data represents mean ± SEM from three replicate cultures from three helminth-free lambs. *** P < 0.001 (one way ANOVA followed by the Tukey post hoc test for pairwise comparison of means).

Article Snippet: To determine the role of interleukin-10 (IL-10) PBMC were cultured in the presence of 5 μg/mL anti-ovine IL-10 monoclonal antibody (mAb) CC320 (AbDserotec, Kidlington, UK) or 5 μg/mL mouse IgG1 isotype control (eBioscience).

Techniques: Neutralization, Cell Culture

Journal: Veterinary Research

Article Title: Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products

doi: 10.1186/1297-9716-44-70

Figure Lengend Snippet: The effects of T. circumcincta infection on Tci - L4 - ES mediated lymphocyte suppression. Seven helminth-naïve lambs were trickle infected with 2000 T . circumcincta L3 three times a week for four weeks. PBMC were harvested at 0, 2, 4 and 6 weeks from the start of infection and cultured for 72 h with PBS alone (PBS), 5 μg/mL Con A (Con A + PBS) or 5 μg/mL Con A + 15 μg/mL Tci -L4-ES (Con A + ES). Proliferation was assessed at 72 h by measurement of [ 3 H] thymidine incorporation. Levels of cytokine present in the cell culture supernatants at 54 h were determined by ELISA. (A) Faecal egg count (FEC) data over the course of the experimental infection. (B) Proliferation of PBMC in response to PBS, Con A + PBS and Con A + ES during experimental T . circumcincta infection. (C) Levels of serum Tci -L4-ES-specific IgA and IgG during experimental T . circumcincta infection. (D - F) Concentrations of IL-4, IL-10 and IFN-γ in PBMC cultures supernatants. Data represents mean ± SEM. a significant difference between Con A + PBS vs. PBS-stimulated cultures; b significant difference between ConA + ES vs. PBS-stimulated cultures; c significant difference between Con A + PBS vs. Con A + ES stimulated cultures; * P < 0.05, ** P < 0.01, *** P < 0.001 (repeated-measures two-way ANOVA followed by the Bonferroni post hoc test for comparison of means).

Article Snippet: To determine the role of interleukin-10 (IL-10) PBMC were cultured in the presence of 5 μg/mL anti-ovine IL-10 monoclonal antibody (mAb) CC320 (AbDserotec, Kidlington, UK) or 5 μg/mL mouse IgG1 isotype control (eBioscience).

Techniques: Infection, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Veterinary Research

Article Title: Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products

doi: 10.1186/1297-9716-44-70

Figure Lengend Snippet: The relationship between Tci -L4 - ES mediated lymphocyte suppression and Tci - L4 - ES antigen - specific lymphocyte proliferation. Five helminth-naïve lambs were trickle infected with 2000 T . circumcincta L3 three times a week for six weeks. PBMC were harvested at 0 and 6 weeks from the start of infection and cultured for 72 h with PBS alone, 5 μg/mL Con A, 5 μg/mL Con A + 30 μg/mL Tci -L4-ES (Con A + ES) or 30 μg/mL heat-inactivated Tci -L4-ES (HiES). Proliferation was assessed at 72 h by measurement of [ 3 H] thymidine incorporation. Levels of IL-10 present in the cell culture supernatants at 54 h were determined by ELISA. (A) Percentage suppression of Con A-induced proliferation of PBMC by ES. (B) Proliferation of PBMC in response to HiES expressed as a stimulation index (SI). (C) Concentration of IL-10 in culture supernatants of PBMC incubated with Con A + ES. (D) Concentration of IL-10 in culture supernatants of PBMC incubated with HiES. Pre = PBMC harvested before infection; Post = PBMC harvested after infection. Open squares represent data from the same animal. * P < 0.05 (students t -test).

Article Snippet: To determine the role of interleukin-10 (IL-10) PBMC were cultured in the presence of 5 μg/mL anti-ovine IL-10 monoclonal antibody (mAb) CC320 (AbDserotec, Kidlington, UK) or 5 μg/mL mouse IgG1 isotype control (eBioscience).

Techniques: Infection, Cell Culture, Enzyme-linked Immunosorbent Assay, Concentration Assay, Incubation