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Illumina Inc cbot
Cbot, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cbot/product/Illumina Inc
Average 99 stars, based on 211 article reviews
Price from $9.99 to $1999.99
cbot - by Bioz Stars, 2020-09
99/100 stars

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Related Articles

Sequencing:

Article Title: Long insert whole genome sequencing for copy number variant and translocation detection
Article Snippet: .. PE sequencing Libraries were used to generate clusters on HiSeq Paired End v3 flowcells on the Illumina cBot using Illumina’s TruSeq PE Cluster Kit v3. ..

Article Title: Changes in cellular microRNA expression induced by porcine circovirus type 2-encoded proteins
Article Snippet: .. Each library (8 pM) was used for cluster generation with a TruSeq SR cluster kit v2 (Illumina) on an Illumina cBot, followed by sequencing on an Illumina Genome Analyzer IIx. ..

Article Title: Seasonal and Regional Differences in Gene Expression in the Brain of a Hibernating Mammal
Article Snippet: .. Cluster generation and sequencing Truseq libraries were hybridized to a paired-end flow cell and individual fragments were clonally amplified by bridge amplification on the Illumina cBot. .. The flow cell was then loaded on the HiSeq 2000 and sequenced using Illumina’s Sequencing by Synthesis (SBS) chemistry.

Concentration Assay:

Article Title: Rapid identification of regulatory microRNAs by miTRAP (miRNA trapping by RNA in vitro affinity purification)
Article Snippet: .. Library DNA at a concentration of 10 pM was clustered using an Illumina cBot according to the SR_Amp_Lin_Block_Hybv8.0 protocol of the manufacturer. .. Sequencing of 50 bp was performed with an Illumina HighScan-SQ sequencer using version 3 chemistry and the version 3 flowcell according to the instructions of the manufacturer.

Flow Cytometry:

Article Title: Seasonal and Regional Differences in Gene Expression in the Brain of a Hibernating Mammal
Article Snippet: .. Cluster generation and sequencing Truseq libraries were hybridized to a paired-end flow cell and individual fragments were clonally amplified by bridge amplification on the Illumina cBot. .. The flow cell was then loaded on the HiSeq 2000 and sequenced using Illumina’s Sequencing by Synthesis (SBS) chemistry.

Amplification:

Article Title: Seasonal and Regional Differences in Gene Expression in the Brain of a Hibernating Mammal
Article Snippet: .. Cluster generation and sequencing Truseq libraries were hybridized to a paired-end flow cell and individual fragments were clonally amplified by bridge amplification on the Illumina cBot. .. The flow cell was then loaded on the HiSeq 2000 and sequenced using Illumina’s Sequencing by Synthesis (SBS) chemistry.

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  • 99
    Illumina Inc illumina cbot
    Setting up lane-by-lane sequencing on an <t>Illumina</t> GAIIx a) <t>cBOT</t> cluster plate set up to cluster lanes 5–8. Lanes 1–4 have been removed using a razor blade and replaced with vials containing HSB. b) The TileSelection section of an XML recipe file for 4-lane sequencing of the flowcell clustered in a. Only lanes 5–8 will be imaged. c) Disconnecting the syringe-pumps. Once the manifold housing is removed, the plunger heads can be unscrewed from the motorized crossbar. Here, pumping has been inactivated on lanes 1 and 2. d) Pause-and-pulse reagent delivery method for reads longer than 40 nt. The left lane is active and the right lane is disconnected. Orange arrows represent flow of new reagent, green arrows represent movement of previous reagent, white circle represents a small air bubble remaining in the disconnected lane. i. During the initial pumping stage, reagent delivered from the inlet (bottom) may be contaminated by fluid from disconnected lanes as small bubbles expand due to negative pressure from the pump. ii. After pumping, a 5-second pause allows a small amount of the current reagent to back-fill through the inlet manifold as pressure equalizes in the system. iii. A 20 μL pulse then finishes delivery with homogenous reagent.
    Illumina Cbot, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 351 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina cbot/product/Illumina Inc
    Average 99 stars, based on 351 article reviews
    Price from $9.99 to $1999.99
    illumina cbot - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    88
    Illumina Inc flowcell follow illumina cbot user guide
    DNA template and RNA transcript of HiTS-RAP. (A) Schematics of the DNA template used for HiTS-RAP and the resulting halted RNA transcript. DNA template encoding the RNA of interest (green) is flanked by the <t>Illumina</t> <t>flowcell</t> adaptor 1 (gray) and T7 RNA polymerase promoter (orange) upstream, and by the Illumina sequencing primer annealing site (purple), Tus-binding Ter site (red), and Illumina flowcell adaptor 2 downstream. Illumina flowcell adaptors 1 and 2 are required for cluster generation on Illumina GA flowcell. T7 RNA polymerase promoter is required for transcription of the RNA of interest. The Illumina sequencing primer is used for sequencing the DNA template of the RNA of interest and serves as a docking site for the T7 RNA polymerase when it is halted. Tus protein binds to Ter site and halts the transcribing RNA polymerase. Direction of transcription and sequencing are indicated by orange and purple arrows, respectively. Tus-bound Ter site that is non-permissive (halting) and permissive (read-through) to RNA polymerase are indicated by solid and open red triangles, respectively. The halted RNA transcript includes a triplet G derived from the T7 promoter, followed by the RNA of interest and some of the Illumina sequencing primer. The 3’-end of RNA transcript, indicated by a dashed line, is inaccessible. (B) Construction of DNA templates for HiTS-RAP. DNA template is constructed by PCR in two steps using 2 sets of nested oligos. Forward oligos introduce T7 promoter (step 1) and Illumina flowcell adaptor (step 2), whereas reverse oligos introduce Illumina sequencing primer (step 1), and Ter site and Illumina flowcell adaptor 2 (step 2). (C) Sequence of the HiTS-RAP DNA template for GFP aptamer. GFP aptamer encoding sequence is in green, and the rest of the sequences are colored as in (A). The transcription start site is indicated by +1 and a broken arrow. (D) Sequence of the halted RNA transcript from GFP aptamer template. Sequences are colored as in (C). Uppercase indicates the region of the halted RNA transcript that is accessible, and the lowercase indicates a region that is likely to be buried in T7 RNA polymerase and thus inaccessible by other proteins.
    Flowcell Follow Illumina Cbot User Guide, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flowcell follow illumina cbot user guide/product/Illumina Inc
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flowcell follow illumina cbot user guide - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    91
    Illumina Inc truseq sr cluster kit v3 cbot hs
    DNA template and RNA transcript of HiTS-RAP. (A) Schematics of the DNA template used for HiTS-RAP and the resulting halted RNA transcript. DNA template encoding the RNA of interest (green) is flanked by the <t>Illumina</t> <t>flowcell</t> adaptor 1 (gray) and T7 RNA polymerase promoter (orange) upstream, and by the Illumina sequencing primer annealing site (purple), Tus-binding Ter site (red), and Illumina flowcell adaptor 2 downstream. Illumina flowcell adaptors 1 and 2 are required for cluster generation on Illumina GA flowcell. T7 RNA polymerase promoter is required for transcription of the RNA of interest. The Illumina sequencing primer is used for sequencing the DNA template of the RNA of interest and serves as a docking site for the T7 RNA polymerase when it is halted. Tus protein binds to Ter site and halts the transcribing RNA polymerase. Direction of transcription and sequencing are indicated by orange and purple arrows, respectively. Tus-bound Ter site that is non-permissive (halting) and permissive (read-through) to RNA polymerase are indicated by solid and open red triangles, respectively. The halted RNA transcript includes a triplet G derived from the T7 promoter, followed by the RNA of interest and some of the Illumina sequencing primer. The 3’-end of RNA transcript, indicated by a dashed line, is inaccessible. (B) Construction of DNA templates for HiTS-RAP. DNA template is constructed by PCR in two steps using 2 sets of nested oligos. Forward oligos introduce T7 promoter (step 1) and Illumina flowcell adaptor (step 2), whereas reverse oligos introduce Illumina sequencing primer (step 1), and Ter site and Illumina flowcell adaptor 2 (step 2). (C) Sequence of the HiTS-RAP DNA template for GFP aptamer. GFP aptamer encoding sequence is in green, and the rest of the sequences are colored as in (A). The transcription start site is indicated by +1 and a broken arrow. (D) Sequence of the halted RNA transcript from GFP aptamer template. Sequences are colored as in (C). Uppercase indicates the region of the halted RNA transcript that is accessible, and the lowercase indicates a region that is likely to be buried in T7 RNA polymerase and thus inaccessible by other proteins.
    Truseq Sr Cluster Kit V3 Cbot Hs, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq sr cluster kit v3 cbot hs/product/Illumina Inc
    Average 91 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    truseq sr cluster kit v3 cbot hs - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Setting up lane-by-lane sequencing on an Illumina GAIIx a) cBOT cluster plate set up to cluster lanes 5–8. Lanes 1–4 have been removed using a razor blade and replaced with vials containing HSB. b) The TileSelection section of an XML recipe file for 4-lane sequencing of the flowcell clustered in a. Only lanes 5–8 will be imaged. c) Disconnecting the syringe-pumps. Once the manifold housing is removed, the plunger heads can be unscrewed from the motorized crossbar. Here, pumping has been inactivated on lanes 1 and 2. d) Pause-and-pulse reagent delivery method for reads longer than 40 nt. The left lane is active and the right lane is disconnected. Orange arrows represent flow of new reagent, green arrows represent movement of previous reagent, white circle represents a small air bubble remaining in the disconnected lane. i. During the initial pumping stage, reagent delivered from the inlet (bottom) may be contaminated by fluid from disconnected lanes as small bubbles expand due to negative pressure from the pump. ii. After pumping, a 5-second pause allows a small amount of the current reagent to back-fill through the inlet manifold as pressure equalizes in the system. iii. A 20 μL pulse then finishes delivery with homogenous reagent.

    Journal: BioTechniques

    Article Title: Lane-by-lane sequencing using Illumina’s Genome Analyzer II

    doi: 10.2144/000114032

    Figure Lengend Snippet: Setting up lane-by-lane sequencing on an Illumina GAIIx a) cBOT cluster plate set up to cluster lanes 5–8. Lanes 1–4 have been removed using a razor blade and replaced with vials containing HSB. b) The TileSelection section of an XML recipe file for 4-lane sequencing of the flowcell clustered in a. Only lanes 5–8 will be imaged. c) Disconnecting the syringe-pumps. Once the manifold housing is removed, the plunger heads can be unscrewed from the motorized crossbar. Here, pumping has been inactivated on lanes 1 and 2. d) Pause-and-pulse reagent delivery method for reads longer than 40 nt. The left lane is active and the right lane is disconnected. Orange arrows represent flow of new reagent, green arrows represent movement of previous reagent, white circle represents a small air bubble remaining in the disconnected lane. i. During the initial pumping stage, reagent delivered from the inlet (bottom) may be contaminated by fluid from disconnected lanes as small bubbles expand due to negative pressure from the pump. ii. After pumping, a 5-second pause allows a small amount of the current reagent to back-fill through the inlet manifold as pressure equalizes in the system. iii. A 20 μL pulse then finishes delivery with homogenous reagent.

    Article Snippet: Flowcells can be clustered on any desired number of lanes using the Illumina cBot, sequenced, stored, and then clustered again on remaining lanes without appreciable reduction in intensity, cluster density or accuracy of the run.

    Techniques: Sequencing, Flow Cytometry

    DNA template and RNA transcript of HiTS-RAP. (A) Schematics of the DNA template used for HiTS-RAP and the resulting halted RNA transcript. DNA template encoding the RNA of interest (green) is flanked by the Illumina flowcell adaptor 1 (gray) and T7 RNA polymerase promoter (orange) upstream, and by the Illumina sequencing primer annealing site (purple), Tus-binding Ter site (red), and Illumina flowcell adaptor 2 downstream. Illumina flowcell adaptors 1 and 2 are required for cluster generation on Illumina GA flowcell. T7 RNA polymerase promoter is required for transcription of the RNA of interest. The Illumina sequencing primer is used for sequencing the DNA template of the RNA of interest and serves as a docking site for the T7 RNA polymerase when it is halted. Tus protein binds to Ter site and halts the transcribing RNA polymerase. Direction of transcription and sequencing are indicated by orange and purple arrows, respectively. Tus-bound Ter site that is non-permissive (halting) and permissive (read-through) to RNA polymerase are indicated by solid and open red triangles, respectively. The halted RNA transcript includes a triplet G derived from the T7 promoter, followed by the RNA of interest and some of the Illumina sequencing primer. The 3’-end of RNA transcript, indicated by a dashed line, is inaccessible. (B) Construction of DNA templates for HiTS-RAP. DNA template is constructed by PCR in two steps using 2 sets of nested oligos. Forward oligos introduce T7 promoter (step 1) and Illumina flowcell adaptor (step 2), whereas reverse oligos introduce Illumina sequencing primer (step 1), and Ter site and Illumina flowcell adaptor 2 (step 2). (C) Sequence of the HiTS-RAP DNA template for GFP aptamer. GFP aptamer encoding sequence is in green, and the rest of the sequences are colored as in (A). The transcription start site is indicated by +1 and a broken arrow. (D) Sequence of the halted RNA transcript from GFP aptamer template. Sequences are colored as in (C). Uppercase indicates the region of the halted RNA transcript that is accessible, and the lowercase indicates a region that is likely to be buried in T7 RNA polymerase and thus inaccessible by other proteins.

    Journal: Nature protocols

    Article Title: Quantitative Assessment of RNA-Protein Interactions with High Throughput Sequencing - RNA Affinity Profiling (HiTS-RAP)

    doi: 10.1038/nprot.2015.074

    Figure Lengend Snippet: DNA template and RNA transcript of HiTS-RAP. (A) Schematics of the DNA template used for HiTS-RAP and the resulting halted RNA transcript. DNA template encoding the RNA of interest (green) is flanked by the Illumina flowcell adaptor 1 (gray) and T7 RNA polymerase promoter (orange) upstream, and by the Illumina sequencing primer annealing site (purple), Tus-binding Ter site (red), and Illumina flowcell adaptor 2 downstream. Illumina flowcell adaptors 1 and 2 are required for cluster generation on Illumina GA flowcell. T7 RNA polymerase promoter is required for transcription of the RNA of interest. The Illumina sequencing primer is used for sequencing the DNA template of the RNA of interest and serves as a docking site for the T7 RNA polymerase when it is halted. Tus protein binds to Ter site and halts the transcribing RNA polymerase. Direction of transcription and sequencing are indicated by orange and purple arrows, respectively. Tus-bound Ter site that is non-permissive (halting) and permissive (read-through) to RNA polymerase are indicated by solid and open red triangles, respectively. The halted RNA transcript includes a triplet G derived from the T7 promoter, followed by the RNA of interest and some of the Illumina sequencing primer. The 3’-end of RNA transcript, indicated by a dashed line, is inaccessible. (B) Construction of DNA templates for HiTS-RAP. DNA template is constructed by PCR in two steps using 2 sets of nested oligos. Forward oligos introduce T7 promoter (step 1) and Illumina flowcell adaptor (step 2), whereas reverse oligos introduce Illumina sequencing primer (step 1), and Ter site and Illumina flowcell adaptor 2 (step 2). (C) Sequence of the HiTS-RAP DNA template for GFP aptamer. GFP aptamer encoding sequence is in green, and the rest of the sequences are colored as in (A). The transcription start site is indicated by +1 and a broken arrow. (D) Sequence of the halted RNA transcript from GFP aptamer template. Sequences are colored as in (C). Uppercase indicates the region of the halted RNA transcript that is accessible, and the lowercase indicates a region that is likely to be buried in T7 RNA polymerase and thus inaccessible by other proteins.

    Article Snippet: 022620511 and 022620623) cBot (Illumina, cat. no. SY-301-2002) CRITICAL: For cluster generation on flowcell follow Illumina cBot™ User Guide (Part # 15006165 Rev.

    Techniques: Sequencing, Binding Assay, Derivative Assay, Construct, Polymerase Chain Reaction, Introduce