cbe 2 693  (ATCC)


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    Structured Review

    ATCC cbe 2 693
    bont/ A-G assay designs.
    Cbe 2 693, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cbe 2 693/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cbe 2 693 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Real-time PCR assays that detect genes for botulinum neurotoxin A–G subtypes"

    Article Title: Real-time PCR assays that detect genes for botulinum neurotoxin A–G subtypes

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2024.1382056


    Figure Legend Snippet: bont/ A-G assay designs.

    Techniques Used: Sequencing, Amplification, Positive Control


    Figure Legend Snippet: Summary of laboratory sensitivity and specificity testing of bont gene RT-PCR assays.

    Techniques Used:

    Sequence alignment of the gene fragment in the 12 bont /E subtypes between the CbE 2.693 forward and reverse primers. The alignment shows exact sequence matches in subtypes bont/ E1-E7, with a single mismatch in the probe for bont/ E9 and mismatches in the reverse primer for bont/ E8, bont/ E11, and bont /E12. The primer sites are highlighted in green and the probe site is highlighted in red.
    Figure Legend Snippet: Sequence alignment of the gene fragment in the 12 bont /E subtypes between the CbE 2.693 forward and reverse primers. The alignment shows exact sequence matches in subtypes bont/ E1-E7, with a single mismatch in the probe for bont/ E9 and mismatches in the reverse primer for bont/ E8, bont/ E11, and bont /E12. The primer sites are highlighted in green and the probe site is highlighted in red.

    Techniques Used: Sequencing

    cbe 2 693 assay  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
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  • About
  • News
  • Press Release
  • Team
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  • 86

    Structured Review

    ATCC cbe 2 693 assay
    bont/ A-G assay designs.
    Cbe 2 693 Assay, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cbe 2 693 assay/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cbe 2 693 assay - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Real-time PCR assays that detect genes for botulinum neurotoxin A–G subtypes"

    Article Title: Real-time PCR assays that detect genes for botulinum neurotoxin A–G subtypes

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2024.1382056


    Figure Legend Snippet: bont/ A-G assay designs.

    Techniques Used: Sequencing, Amplification, Positive Control


    Figure Legend Snippet: Summary of laboratory sensitivity and specificity testing of bont gene RT-PCR assays.

    Techniques Used:

    Sequence alignment of the gene fragment in the 12 bont /E subtypes between the CbE 2.693 forward and reverse primers. The alignment shows exact sequence matches in subtypes bont/ E1-E7, with a single mismatch in the probe for bont/ E9 and mismatches in the reverse primer for bont/ E8, bont/ E11, and bont /E12. The primer sites are highlighted in green and the probe site is highlighted in red.
    Figure Legend Snippet: Sequence alignment of the gene fragment in the 12 bont /E subtypes between the CbE 2.693 forward and reverse primers. The alignment shows exact sequence matches in subtypes bont/ E1-E7, with a single mismatch in the probe for bont/ E9 and mismatches in the reverse primer for bont/ E8, bont/ E11, and bont /E12. The primer sites are highlighted in green and the probe site is highlighted in red.

    Techniques Used: Sequencing

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  • 86
    ATCC cbe 2 693
    bont/ A-G assay designs.
    Cbe 2 693, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cbe 2 693/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cbe 2 693 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    ATCC cbe 2 693 assay
    bont/ A-G assay designs.
    Cbe 2 693 Assay, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cbe 2 693 assay/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cbe 2 693 assay - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Journal: Frontiers in Microbiology

    Article Title: Real-time PCR assays that detect genes for botulinum neurotoxin A–G subtypes

    doi: 10.3389/fmicb.2024.1382056

    Figure Lengend Snippet: bont/ A-G assay designs.

    Article Snippet: While sensitivity testing of DNA from BoNT/E1 ATCC 17852 with CbE 2.693 resulted in a failure to detect the bont/ E1 gene, a positive result (Ct 29.9) was observed when this DNA preparation was tested at 100 pg.

    Techniques: Sequencing, Amplification, Positive Control

    Sequence alignment of the gene fragment in the 12 bont /E subtypes between the CbE 2.693 forward and reverse primers. The alignment shows exact sequence matches in subtypes bont/ E1-E7, with a single mismatch in the probe for bont/ E9 and mismatches in the reverse primer for bont/ E8, bont/ E11, and bont /E12. The primer sites are highlighted in green and the probe site is highlighted in red.

    Journal: Frontiers in Microbiology

    Article Title: Real-time PCR assays that detect genes for botulinum neurotoxin A–G subtypes

    doi: 10.3389/fmicb.2024.1382056

    Figure Lengend Snippet: Sequence alignment of the gene fragment in the 12 bont /E subtypes between the CbE 2.693 forward and reverse primers. The alignment shows exact sequence matches in subtypes bont/ E1-E7, with a single mismatch in the probe for bont/ E9 and mismatches in the reverse primer for bont/ E8, bont/ E11, and bont /E12. The primer sites are highlighted in green and the probe site is highlighted in red.

    Article Snippet: While sensitivity testing of DNA from BoNT/E1 ATCC 17852 with CbE 2.693 resulted in a failure to detect the bont/ E1 gene, a positive result (Ct 29.9) was observed when this DNA preparation was tested at 100 pg.

    Techniques: Sequencing

    Journal: Frontiers in Microbiology

    Article Title: Real-time PCR assays that detect genes for botulinum neurotoxin A–G subtypes

    doi: 10.3389/fmicb.2024.1382056

    Figure Lengend Snippet: bont/ A-G assay designs.

    Article Snippet: Unlike CbE 2.0, the CbE 2.693 assay yielded results that were variable and less sensitive, with a complete failure to detect bont genes from one subtype variant (BoNT/E1 ATCC 17852), and variable results with several other DNA preparations including detection failures and Ct values of 36.1–39.8 when 1 pg. of target DNA was used in testing.

    Techniques: Sequencing, Amplification, Positive Control

    Sequence alignment of the gene fragment in the 12 bont /E subtypes between the CbE 2.693 forward and reverse primers. The alignment shows exact sequence matches in subtypes bont/ E1-E7, with a single mismatch in the probe for bont/ E9 and mismatches in the reverse primer for bont/ E8, bont/ E11, and bont /E12. The primer sites are highlighted in green and the probe site is highlighted in red.

    Journal: Frontiers in Microbiology

    Article Title: Real-time PCR assays that detect genes for botulinum neurotoxin A–G subtypes

    doi: 10.3389/fmicb.2024.1382056

    Figure Lengend Snippet: Sequence alignment of the gene fragment in the 12 bont /E subtypes between the CbE 2.693 forward and reverse primers. The alignment shows exact sequence matches in subtypes bont/ E1-E7, with a single mismatch in the probe for bont/ E9 and mismatches in the reverse primer for bont/ E8, bont/ E11, and bont /E12. The primer sites are highlighted in green and the probe site is highlighted in red.

    Article Snippet: Unlike CbE 2.0, the CbE 2.693 assay yielded results that were variable and less sensitive, with a complete failure to detect bont genes from one subtype variant (BoNT/E1 ATCC 17852), and variable results with several other DNA preparations including detection failures and Ct values of 36.1–39.8 when 1 pg. of target DNA was used in testing.

    Techniques: Sequencing