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Santa Cruz Biotechnology caveolin 1 expression antibodies
Knockdown of <t>caveolin-1</t> attenuates PKC-mediated down regulation of NET function. Immunoblotting demonstrate silencing of caveolin-1 expression by stable expression of Cav1 shRNA construct (PC12-Cav1 shRNA) or MKP3 (PC12-MKP3) and successful re-introduction of caveolin-1 in MKP3 expressing cells (PC12-MKP3-HACav1) (A) . Following Cav1 silencing or re-introduction PKC was activated by PMA for 30 min and NET-specific radiolabeled uptake was performed as above (B) . Results are displayed as percentage uptake following PMA treatment compared to vehicle treated control of respective cell type. Expression of MKP3 or silencing of Caveolin1 by the shRNA construct attenuates NET downregulation following PKC activation to a similar degree (B) . Re-introduction of caveolin1 into MKP3 expressing PC12 cells does not recover the PKC-mediated down regulation (B) . All experiments were performed in triplicate in three independent experiments. Results from three independent experiments were analyzed by two-way ANOVA with Bonferroni post hoc analysis (** p
Caveolin 1 Expression Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "MAP Kinase Phosphatase 3 (MKP3) Preserves Norepinephrine Transporter Activity by Modulating ERK1/2 Kinase-Mediated Gene Expression"

Article Title: MAP Kinase Phosphatase 3 (MKP3) Preserves Norepinephrine Transporter Activity by Modulating ERK1/2 Kinase-Mediated Gene Expression

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2017.00253

Knockdown of caveolin-1 attenuates PKC-mediated down regulation of NET function. Immunoblotting demonstrate silencing of caveolin-1 expression by stable expression of Cav1 shRNA construct (PC12-Cav1 shRNA) or MKP3 (PC12-MKP3) and successful re-introduction of caveolin-1 in MKP3 expressing cells (PC12-MKP3-HACav1) (A) . Following Cav1 silencing or re-introduction PKC was activated by PMA for 30 min and NET-specific radiolabeled uptake was performed as above (B) . Results are displayed as percentage uptake following PMA treatment compared to vehicle treated control of respective cell type. Expression of MKP3 or silencing of Caveolin1 by the shRNA construct attenuates NET downregulation following PKC activation to a similar degree (B) . Re-introduction of caveolin1 into MKP3 expressing PC12 cells does not recover the PKC-mediated down regulation (B) . All experiments were performed in triplicate in three independent experiments. Results from three independent experiments were analyzed by two-way ANOVA with Bonferroni post hoc analysis (** p
Figure Legend Snippet: Knockdown of caveolin-1 attenuates PKC-mediated down regulation of NET function. Immunoblotting demonstrate silencing of caveolin-1 expression by stable expression of Cav1 shRNA construct (PC12-Cav1 shRNA) or MKP3 (PC12-MKP3) and successful re-introduction of caveolin-1 in MKP3 expressing cells (PC12-MKP3-HACav1) (A) . Following Cav1 silencing or re-introduction PKC was activated by PMA for 30 min and NET-specific radiolabeled uptake was performed as above (B) . Results are displayed as percentage uptake following PMA treatment compared to vehicle treated control of respective cell type. Expression of MKP3 or silencing of Caveolin1 by the shRNA construct attenuates NET downregulation following PKC activation to a similar degree (B) . Re-introduction of caveolin1 into MKP3 expressing PC12 cells does not recover the PKC-mediated down regulation (B) . All experiments were performed in triplicate in three independent experiments. Results from three independent experiments were analyzed by two-way ANOVA with Bonferroni post hoc analysis (** p

Techniques Used: Expressing, shRNA, Construct, Activation Assay

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Expressing:

Article Title: MAP Kinase Phosphatase 3 (MKP3) Preserves Norepinephrine Transporter Activity by Modulating ERK1/2 Kinase-Mediated Gene Expression
Article Snippet: .. To detect levels of caveolin-1 expression antibodies from Santa Cruz Biotechnology was used (N-20, sc-894). .. To detect levels of caveolin-2 expression antibodies from Abcam (Cambridge, MA, USA) was used (ab2911).

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    Santa Cruz Biotechnology caveolin 1α n20
    Immunostaining patterns for <t>caveolin-1α</t> and PECAM-1 in lung sections. Cav-1α staining was compared at E14.5, E17.5 and E20.5 stages of development. Both the epithelium and mesenchyme were negative for cav-1α at E14.5. Developing blood vessels (red arrowheads) and capillaries (arrows) (panels A and B) were stained positively for cav-1α, but the staining patterns between wild-type (+/+) and knockout (-/-) lungs were not altered. Cav-1α was also detected at the proximal bronchiole sub-epithelial matrices (panels A-D, black arrowheads). At E17.5, the airway epithelium, negative for cav-1α staining, was surrounded by rings of cav-1α positively stained capillary networks (panels C and D, arrows). Cav-1α expression patterns in lungs from knockout embryos at E17.5 were similar to wild-type embryos, but relative less capillary bed was noted in knockout lungs (cf. panels C and D). At E20.5 of wild-type lungs, both cav-1α and PECAM-1 staining demonstrated an extensive vascular network in the well developed saccules, with staining observed in close proximity to flat epithelial cells lining the air spaces (panels E and G, arrows). However, delayed vascular development was observed in knockout lungs, showing capillaries (panels F and H, arrows) of the knockout lungs at E20.5 were embedded in a relatively thick mesenchyme around the undeveloped epithelial tubules in which the epithelial cells were remained cuboidal (arrowheads). Original magnification: A-D are 20X; E-H are 40X.
    Caveolin 1α N20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Santa Cruz Biotechnology caveolin 1
    <t>Caveolin-1</t> and SK Hep1 cells. A : Western blot for caveolin-1 showing positive expression (arrow). B and C : immunogold showing expression of caveolin-1 (arrows) in SK Hep1 cells in longitudinal ( B ) and transected ( C ) vesicles (Bars = 100 nm).
    Caveolin 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 76 article reviews
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    Image Search Results


    Immunostaining patterns for caveolin-1α and PECAM-1 in lung sections. Cav-1α staining was compared at E14.5, E17.5 and E20.5 stages of development. Both the epithelium and mesenchyme were negative for cav-1α at E14.5. Developing blood vessels (red arrowheads) and capillaries (arrows) (panels A and B) were stained positively for cav-1α, but the staining patterns between wild-type (+/+) and knockout (-/-) lungs were not altered. Cav-1α was also detected at the proximal bronchiole sub-epithelial matrices (panels A-D, black arrowheads). At E17.5, the airway epithelium, negative for cav-1α staining, was surrounded by rings of cav-1α positively stained capillary networks (panels C and D, arrows). Cav-1α expression patterns in lungs from knockout embryos at E17.5 were similar to wild-type embryos, but relative less capillary bed was noted in knockout lungs (cf. panels C and D). At E20.5 of wild-type lungs, both cav-1α and PECAM-1 staining demonstrated an extensive vascular network in the well developed saccules, with staining observed in close proximity to flat epithelial cells lining the air spaces (panels E and G, arrows). However, delayed vascular development was observed in knockout lungs, showing capillaries (panels F and H, arrows) of the knockout lungs at E20.5 were embedded in a relatively thick mesenchyme around the undeveloped epithelial tubules in which the epithelial cells were remained cuboidal (arrowheads). Original magnification: A-D are 20X; E-H are 40X.

    Journal: BMC Developmental Biology

    Article Title: Late gestational lung hypoplasia in a mouse model of the Smith-Lemli-Opitz syndrome

    doi: 10.1186/1471-213X-4-1

    Figure Lengend Snippet: Immunostaining patterns for caveolin-1α and PECAM-1 in lung sections. Cav-1α staining was compared at E14.5, E17.5 and E20.5 stages of development. Both the epithelium and mesenchyme were negative for cav-1α at E14.5. Developing blood vessels (red arrowheads) and capillaries (arrows) (panels A and B) were stained positively for cav-1α, but the staining patterns between wild-type (+/+) and knockout (-/-) lungs were not altered. Cav-1α was also detected at the proximal bronchiole sub-epithelial matrices (panels A-D, black arrowheads). At E17.5, the airway epithelium, negative for cav-1α staining, was surrounded by rings of cav-1α positively stained capillary networks (panels C and D, arrows). Cav-1α expression patterns in lungs from knockout embryos at E17.5 were similar to wild-type embryos, but relative less capillary bed was noted in knockout lungs (cf. panels C and D). At E20.5 of wild-type lungs, both cav-1α and PECAM-1 staining demonstrated an extensive vascular network in the well developed saccules, with staining observed in close proximity to flat epithelial cells lining the air spaces (panels E and G, arrows). However, delayed vascular development was observed in knockout lungs, showing capillaries (panels F and H, arrows) of the knockout lungs at E20.5 were embedded in a relatively thick mesenchyme around the undeveloped epithelial tubules in which the epithelial cells were remained cuboidal (arrowheads). Original magnification: A-D are 20X; E-H are 40X.

    Article Snippet: Sections were blocked with 5% normal serum for 2 h, washed in PBS and incubated with antibodies against Shh-N19 (1:100), Patched (1:100), Smo-N19 (1:100), Gli1 (1:100), Gli3-N19 (1:100), SP-C C19 (1:200), caveolin 1α-N20 (1:200), aquaporin 5 (AQP5, 1:200), megalin (1:200), PECAM-1 (1:200) (Santa Cruz Biotechnology, Santa Cruz, CA) or T1α (mAB 8.1.1, 1:1000 dilution, Developmental Studies Hybridoma Bank, Iowa City, IA) overnight at 4° C in a humidified chamber.

    Techniques: Immunostaining, Staining, Knock-Out, Expressing

    Caveolin-1 and SK Hep1 cells. A : Western blot for caveolin-1 showing positive expression (arrow). B and C : immunogold showing expression of caveolin-1 (arrows) in SK Hep1 cells in longitudinal ( B ) and transected ( C ) vesicles (Bars = 100 nm).

    Journal:

    Article Title: The response of fenestrations, actin, and caveolin-1 to vascular endothelial growth factor in SK Hep1 cells

    doi: 10.1152/ajpgi.00069.2008

    Figure Lengend Snippet: Caveolin-1 and SK Hep1 cells. A : Western blot for caveolin-1 showing positive expression (arrow). B and C : immunogold showing expression of caveolin-1 (arrows) in SK Hep1 cells in longitudinal ( B ) and transected ( C ) vesicles (Bars = 100 nm).

    Article Snippet: Immunogold was performed to localize the distribution of caveolin-1 in SK Hep1 cells.

    Techniques: Western Blot, Expressing

    Fluorescent images of SK Hep1 cells transfected stably with GFP-actin ( A ) and caveolin-1 ( D ). The effect of VEGF at 1 h ( B , E ) and 12 h ( C , F ) is shown.

    Journal:

    Article Title: The response of fenestrations, actin, and caveolin-1 to vascular endothelial growth factor in SK Hep1 cells

    doi: 10.1152/ajpgi.00069.2008

    Figure Lengend Snippet: Fluorescent images of SK Hep1 cells transfected stably with GFP-actin ( A ) and caveolin-1 ( D ). The effect of VEGF at 1 h ( B , E ) and 12 h ( C , F ) is shown.

    Article Snippet: Immunogold was performed to localize the distribution of caveolin-1 in SK Hep1 cells.

    Techniques: Transfection, Stable Transfection