Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Silencing the ACAT1 Gene in Human SH-SY5Y Neuroblastoma Cells Inhibits the Expression of Cyclo-Oxygenase 2 (COX2) and Reduces β-Amyloid-Induced Toxicity Due to Activation of Protein Kinase C (PKC) and ERK

doi: 10.12659/MSM.912862

Figure Lengend Snippet: Primary antibodies used in the present study.

Article Snippet: Rabbit anti-Cacna1b , 1: 1000 , Alomone Labs, Jerusalem, Israel.

Techniques:

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Silencing the ACAT1 Gene in Human SH-SY5Y Neuroblastoma Cells Inhibits the Expression of Cyclo-Oxygenase 2 (COX2) and Reduces β-Amyloid-Induced Toxicity Due to Activation of Protein Kinase C (PKC) and ERK

doi: 10.12659/MSM.912862

Figure Lengend Snippet: Effects of knockdown ACAT1 on CACNAs, PKC, and ERK phosphorylation and in Aβ-induced SH-SY5Y cells. ( A ) Western blot results showed that the relative expression of CACNA1b and CACNA2d1 was increased significantly, while the total PKC and the ratio of phospho-ERK (p-ERK)/ERK were decreased significantly after Aβ 25-35 treatment. ( B ) Knockdown of ACAT1 rescued the reduction of PKC and phosphorylation level of ERK, and also retarded the upregulation of CACNA1b and CACNA2d1 induced by Aβ. (** P <0.01 and * P <0.05).

Article Snippet: Rabbit anti-Cacna1b , 1: 1000 , Alomone Labs, Jerusalem, Israel.

Techniques: Western Blot, Expressing

Journal: Molecular Neurobiology

Article Title: Muscarinic Receptors in Developmental Axonal Competition at the Neuromuscular Junction

doi: 10.1007/s12035-022-03154-1

Figure Lengend Snippet: Substances list. Muscarinic, purinergic, TrkB, PKC, PKA substances, calcium channel modulators, calcium ions modulators, and their targets. Stock solutions were prepared using PBS or DMSO in accordance with the commercial product information after preparing the working solution

Article Snippet: (2R)-2-[(6-{[(5-Methylthiophen-2-yl) methyl]amino}-9-propyl-9H-purin-2-yl)amino]butan-1-ol , GV-58 , CaV2.2 and CaV2.1 Ca 2+ Channels activator , G-140, Alomone , 20 mM , 20 µM.

Techniques: Recombinant

Journal: Molecular Neurobiology

Article Title: Muscarinic Receptors in Developmental Axonal Competition at the Neuromuscular Junction

doi: 10.1007/s12035-022-03154-1

Figure Lengend Snippet: Substances list. Muscarinic, purinergic, TrkB, PKC, PKA substances, calcium channel modulators, calcium ions modulators, and their targets. Stock solutions were prepared using PBS or DMSO in accordance with the commercial product information after preparing the working solution

Article Snippet: (2R)-2-[(6-{[(5-Methylthiophen-2-yl) methyl]amino}-9-propyl-9H-purin-2-yl)amino]butan-1-ol , GV-58 , CaV2.2 and CaV2.1 Ca 2+ Channels activator , G-140, Alomone , 20 mM , 20 µM.

Techniques: Recombinant

Journal: PLoS ONE

Article Title: Differential Calcium Signaling Mediated by Voltage-Gated Calcium Channels in Rat Retinal Ganglion Cells and Their Unmyelinated Axons

doi: 10.1371/journal.pone.0084507

Figure Lengend Snippet: Ca channel α 1 subunits co-express with the ganglion cell antibody RBPMS in vertical sections showing the inner plexiform layer (IPL), the ganglion cell layer (GCL) and the nerve fiber layer (NFL). ( Top row ) Double immunostaining with α1C subunit and RBPMS antibodies shows colocalization in the RGC somata (filled arrow). RGC axons (arrowhead) as well as putative Müller and neuronal cell processes in the IPL showed immunostaining. Control preadsorption of α1C antibody with the immunization peptide showed no staining. ( Second row ) Double immunostaining with α1D subunit and RBPMS antibodies shows colocalization in the RGC somata (filled arrow). Numerous processes in the IPL as well as the RGC axons throughout the NFL (arrowhead) were also labelled. Control preadsorption of α1D antibody with the immunization peptide showed no staining. ( Third row ) Double immunostaining with α1B subunit and RBPMS antibodies shows colocalization in RGC somata (filled arrow). Processes in the IPL, putative displaced amacrine cells in the GCL (open arrows) and RGC axons throughout the NFL (arrowheads) showed immunostaining. Control preadsorption of α1B antibody with the immunization peptide showed no staining. ( Bottom row ) Double immunostaining with α1A subunit and RBPMS antibodies shows colocalization in the RGC somata (filled arrow). Labelling in the IPL and in putative displaced amacrine cells in the GCL (open arrows) was also seen. Control preadsorption of α1A antibody with the immunization peptide showed no staining. Scale bar is 20 µm.

Article Snippet: The following polyclonal antibodies were used to obtain staining of the α1 subunits: α1C (Cav1.2, Alomone, ACC-003, 1∶1000, Jerusalem, Israel), α1D (Cav1.3, Sigma, C1728, St. Louis, MO, 1∶100), α1A (Cav2.1, Synaptic Systems, 152–103, 1∶7500, Göttingen, Germany), α1B (Cav2.2, Alomone, ACC-002, 1∶1000, Jerusalem, Israel).

Techniques: Double Immunostaining, Immunostaining, Staining

Journal: PLoS ONE

Article Title: Differential Calcium Signaling Mediated by Voltage-Gated Calcium Channels in Rat Retinal Ganglion Cells and Their Unmyelinated Axons

doi: 10.1371/journal.pone.0084507

Figure Lengend Snippet: R at wholemount retina labelled with RBPMS (red), α1B VGCC subunit (green), and NF-M (white) antibodies. α1B colocalized with RBPMS in RGC somata (filled arrow), NF-M in RGC axons (arrowhead) and putative displaced amacrine cells (open arrow). Stack of six optical sections each of 0.3 µm thickness. Scale bar is 20 µm.

Article Snippet: The following polyclonal antibodies were used to obtain staining of the α1 subunits: α1C (Cav1.2, Alomone, ACC-003, 1∶1000, Jerusalem, Israel), α1D (Cav1.3, Sigma, C1728, St. Louis, MO, 1∶100), α1A (Cav2.1, Synaptic Systems, 152–103, 1∶7500, Göttingen, Germany), α1B (Cav2.2, Alomone, ACC-002, 1∶1000, Jerusalem, Israel).

Techniques:

Journal: Molecular Neurobiology

Article Title: Involvement of the Voltage-Gated Calcium Channels L- P/Q- and N-Types in Synapse Elimination During Neuromuscular Junction Development

doi: 10.1007/s12035-022-02818-2

Figure Lengend Snippet: a Representative confocal image of a nerve terminal arborization. Singly, dually, and innervated by three or more axons NMJs from YFP muscles and also images of the morphologic maturation (S1, the most inmature, and S4, almost fully differentiated, stages) of the postsynaptic clusters from P9 mice. The bar indicates 10 μm. b Confocal immunofluorescence location of α 1D L-, N-, and P/Q-type voltage-dependent calcium channels (VDCCs) at the NMJ. Triple labeling of VDCCs (green fluorescence) with syntaxin (blue fluorescence) and nAChR-α-bungarotoxin (red fluorescence) in merge images. Figure shows the presence of α 1D L-, N-, and P/Q-type-VDCC (in green) in the nerve terminal of P9 Levator auris longus (LAL) muscle endplates. The bar indicates 10 μm

Article Snippet: Muscles were incubated overnight at 4 °C with anti-Ca V 1.3 (CACNA1D) antibody voltage-dependent L-type calcium channel subunit α 1D (1/100; ACC-005, Alomone Labs, Jerusalem, Israel); anti-Ca V 2.1 (CACNA1A) antibody voltage-dependent P/Q-type calcium channel subunit α 1A (1/100; ACC-001, Alomone Labs, Jerusalem, Israel); anti-Ca V 2.2 (CACNA1B) antibody voltage-dependent N-type calcium channel subunit α 1B (1/100; ACC1-002, Alomone Labs, Jerusalem, Israel), and anti-mouse syntaxin (1/1000, S066, Sigma, St Louis, MO, USA).

Techniques: Immunofluorescence, Labeling, Fluorescence