rabbit anti cav1 2 primary antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti cav1 2 primary antibody
    <t>Cav1.2</t> is expressed in the embryonic mPFC. A , Representative images of histologic sections from Nkx2.1/Ai14 E16.5 mouse brain ( A1 ) immunostained for Cav1.2 ( A2 ) and overlayed with images of DAPI counterstaining and Nkx2.1/tdTomato-fluorescent GINs ( A3 ). Images were captured at 10× magnification on a spinning disk confocal microscope. B , Representative images at 40× magnification. These images are magnified images of the area demarcated by the white box in A1–A3 . C , Representative images of no primary antibody negative control of Cav1.2 staining.
    Rabbit Anti Cav1 2 Primary Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cav1 2 primary antibody/product/Alomone Labs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cav1 2 primary antibody - by Bioz Stars, 2022-10
    95/100 stars

    Images

    1) Product Images from "L-Type Calcium Channels Contribute to Ethanol-Induced Aberrant Tangential Migration of Primordial Cortical GABAergic Interneurons in the Embryonic Medial Prefrontal Cortex"

    Article Title: L-Type Calcium Channels Contribute to Ethanol-Induced Aberrant Tangential Migration of Primordial Cortical GABAergic Interneurons in the Embryonic Medial Prefrontal Cortex

    Journal: eNeuro

    doi: 10.1523/ENEURO.0359-21.2021

    Cav1.2 is expressed in the embryonic mPFC. A , Representative images of histologic sections from Nkx2.1/Ai14 E16.5 mouse brain ( A1 ) immunostained for Cav1.2 ( A2 ) and overlayed with images of DAPI counterstaining and Nkx2.1/tdTomato-fluorescent GINs ( A3 ). Images were captured at 10× magnification on a spinning disk confocal microscope. B , Representative images at 40× magnification. These images are magnified images of the area demarcated by the white box in A1–A3 . C , Representative images of no primary antibody negative control of Cav1.2 staining.
    Figure Legend Snippet: Cav1.2 is expressed in the embryonic mPFC. A , Representative images of histologic sections from Nkx2.1/Ai14 E16.5 mouse brain ( A1 ) immunostained for Cav1.2 ( A2 ) and overlayed with images of DAPI counterstaining and Nkx2.1/tdTomato-fluorescent GINs ( A3 ). Images were captured at 10× magnification on a spinning disk confocal microscope. B , Representative images at 40× magnification. These images are magnified images of the area demarcated by the white box in A1–A3 . C , Representative images of no primary antibody negative control of Cav1.2 staining.

    Techniques Used: Microscopy, Negative Control, Staining

    Prenatal ethanol exposure does not alter Cav1.2 expression. A , Representative images of Cav1.2 staining overlayed with DAPI and Nkx2.1 in the mPFC of control ( A1 ) and ethanol-fed ( A2 ) E16.5 mouse brain. B , Quantification of fluorescence intensity ratio of Cav1.2 to Nkx2.1 in the mPFC of control and ethanol-treated mice. Unpaired t test. For statistical details, see Results.
    Figure Legend Snippet: Prenatal ethanol exposure does not alter Cav1.2 expression. A , Representative images of Cav1.2 staining overlayed with DAPI and Nkx2.1 in the mPFC of control ( A1 ) and ethanol-fed ( A2 ) E16.5 mouse brain. B , Quantification of fluorescence intensity ratio of Cav1.2 to Nkx2.1 in the mPFC of control and ethanol-treated mice. Unpaired t test. For statistical details, see Results.

    Techniques Used: Expressing, Staining, Fluorescence, Mouse Assay

    2) Product Images from "The impact of age and frailty on ventricular structure and function in C57BL/6J mice"

    Article Title: The impact of age and frailty on ventricular structure and function in C57BL/6J mice

    Journal: The Journal of Physiology

    doi: 10.1113/JP274134

    Frailty grades the age‐dependent decline in expression of Cav1.2 protein in the mouse heart A , representative Western blots for Cav1.2 protein expression in mice with varying FIs. Ponceau S staining was used as a loading control in all experiments (lower panels). B , mean expression of Cav1.2 decreased with age in the mouse heart. C , cardiac Cav1.2 expression was closely graded by frailty score in the mouse. Differences between age groups were assessed using a Mann–Whitney Rank Sum test and correlations were evaluated with linear regression analysis ( n = 8 adult and 8 aged hearts). Filled symbols indicate adult mice and open symbols indicate aged mice.
    Figure Legend Snippet: Frailty grades the age‐dependent decline in expression of Cav1.2 protein in the mouse heart A , representative Western blots for Cav1.2 protein expression in mice with varying FIs. Ponceau S staining was used as a loading control in all experiments (lower panels). B , mean expression of Cav1.2 decreased with age in the mouse heart. C , cardiac Cav1.2 expression was closely graded by frailty score in the mouse. Differences between age groups were assessed using a Mann–Whitney Rank Sum test and correlations were evaluated with linear regression analysis ( n = 8 adult and 8 aged hearts). Filled symbols indicate adult mice and open symbols indicate aged mice.

    Techniques Used: Expressing, Western Blot, Mouse Assay, Staining, MANN-WHITNEY

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    Alomone Labs cav1 2
    <t>Cav1.2</t> knock-down prevents astrocyte activation after scratch
    Cav1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cav1 2/product/Alomone Labs
    Average 95 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    cav1 2 - by Bioz Stars, 2022-10
    95/100 stars
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    Image Search Results


    Cav1.2 knock-down prevents astrocyte activation after scratch

    Journal: Glia

    Article Title: L-type voltage-operated calcium channels contribute to astrocyte activation in vitro

    doi: 10.1002/glia.23013

    Figure Lengend Snippet: Cav1.2 knock-down prevents astrocyte activation after scratch

    Article Snippet: In contrast, RT-PCR, western blot and immunocytochemistry experiments show that Cav1.2 and Cav1.3 are highly expressed in cortical astrocytes ( ). shows representative images of labeled cultured astrocytes at 6 days in vitro ( DIV ); while GFAP abundantly labeled cell processes and cell bodies, labeling with Cav1.2 and Cav1.3 antibodies displayed a punctate staining on the cells surface ( ).

    Techniques: Activation Assay

    LPS enhance the activity and the expression of Cav1.2 Ca ++ channels in astrocytes

    Journal: Glia

    Article Title: L-type voltage-operated calcium channels contribute to astrocyte activation in vitro

    doi: 10.1002/glia.23013

    Figure Lengend Snippet: LPS enhance the activity and the expression of Cav1.2 Ca ++ channels in astrocytes

    Article Snippet: In contrast, RT-PCR, western blot and immunocytochemistry experiments show that Cav1.2 and Cav1.3 are highly expressed in cortical astrocytes ( ). shows representative images of labeled cultured astrocytes at 6 days in vitro ( DIV ); while GFAP abundantly labeled cell processes and cell bodies, labeling with Cav1.2 and Cav1.3 antibodies displayed a punctate staining on the cells surface ( ).

    Techniques: Activity Assay, Expressing

    Cav1.2 KO astrocytes are not sensitive to LPS

    Journal: Glia

    Article Title: L-type voltage-operated calcium channels contribute to astrocyte activation in vitro

    doi: 10.1002/glia.23013

    Figure Lengend Snippet: Cav1.2 KO astrocytes are not sensitive to LPS

    Article Snippet: In contrast, RT-PCR, western blot and immunocytochemistry experiments show that Cav1.2 and Cav1.3 are highly expressed in cortical astrocytes ( ). shows representative images of labeled cultured astrocytes at 6 days in vitro ( DIV ); while GFAP abundantly labeled cell processes and cell bodies, labeling with Cav1.2 and Cav1.3 antibodies displayed a punctate staining on the cells surface ( ).

    Techniques:

    Cav1.2 knock-down prevents astrocyte activation by LPS

    Journal: Glia

    Article Title: L-type voltage-operated calcium channels contribute to astrocyte activation in vitro

    doi: 10.1002/glia.23013

    Figure Lengend Snippet: Cav1.2 knock-down prevents astrocyte activation by LPS

    Article Snippet: In contrast, RT-PCR, western blot and immunocytochemistry experiments show that Cav1.2 and Cav1.3 are highly expressed in cortical astrocytes ( ). shows representative images of labeled cultured astrocytes at 6 days in vitro ( DIV ); while GFAP abundantly labeled cell processes and cell bodies, labeling with Cav1.2 and Cav1.3 antibodies displayed a punctate staining on the cells surface ( ).

    Techniques: Activation Assay

    Expressions of calcium handling proteins. The confocal image showed fluorescein isothiocyanate (green)-labeled Cav1.2, SERCA2a, and NCX1 expressed in isolated rat cardiac ventricular myocytes in SO (left panel), HF (middle panel), and NRG group (right panel). Nuclei were stained with DAPI (blue). The scale bar represents 20 μm (n = 5 for each set of staining).

    Journal: Frontiers in Pharmacology

    Article Title: Neuregulin-1β Partially Improves Cardiac Function in Volume-Overload Heart Failure Through Regulation of Abnormal Calcium Handling

    doi: 10.3389/fphar.2019.00616

    Figure Lengend Snippet: Expressions of calcium handling proteins. The confocal image showed fluorescein isothiocyanate (green)-labeled Cav1.2, SERCA2a, and NCX1 expressed in isolated rat cardiac ventricular myocytes in SO (left panel), HF (middle panel), and NRG group (right panel). Nuclei were stained with DAPI (blue). The scale bar represents 20 μm (n = 5 for each set of staining).

    Article Snippet: Anti-Cav1.2, anti-SERCA2a, and anti-NCX1 antibody were purchased from Alomone (Jerusalem, ACC-003, Israel), Millipore (MA, AB3516P, USA), and Santa Cruz Biotechnology (CA, SC-8094, USA), and secondary antibodies, anti-rabbit488 conjugated antibody was purchased from Santa Cruz Biotechnology (CA, sc-362262, USA).

    Techniques: Labeling, Isolation, Staining

    Quantitative analysis of calcium handling proteins expressions. (A) Representative expression of Cav1.2, SERCA2a, and NCX1, β-actin as loading controls. (B) Comparison of Cav1.2 protein expression (n = 3 rats). (C) Comparison of SERCA2a protein expression (n = 4 rats). (D) Comparison of NCX1 protein expression (n = 4 rats). * P

    Journal: Frontiers in Pharmacology

    Article Title: Neuregulin-1β Partially Improves Cardiac Function in Volume-Overload Heart Failure Through Regulation of Abnormal Calcium Handling

    doi: 10.3389/fphar.2019.00616

    Figure Lengend Snippet: Quantitative analysis of calcium handling proteins expressions. (A) Representative expression of Cav1.2, SERCA2a, and NCX1, β-actin as loading controls. (B) Comparison of Cav1.2 protein expression (n = 3 rats). (C) Comparison of SERCA2a protein expression (n = 4 rats). (D) Comparison of NCX1 protein expression (n = 4 rats). * P

    Article Snippet: Anti-Cav1.2, anti-SERCA2a, and anti-NCX1 antibody were purchased from Alomone (Jerusalem, ACC-003, Israel), Millipore (MA, AB3516P, USA), and Santa Cruz Biotechnology (CA, SC-8094, USA), and secondary antibodies, anti-rabbit488 conjugated antibody was purchased from Santa Cruz Biotechnology (CA, sc-362262, USA).

    Techniques: Expressing

    Location of antibody epitopes. Shown is a schematic of the Ca v 1.2 α 1 1.2 subunit, in which regions used as immunogens for the depicted antibodies are identified by arrows. Exact residues are listed in the table and numbered according to α 1 1.2 given in Gene Bank Accession number CAA33546. FP1, CNC1, and ACC-003 are directed against the loop between domains II and III, pS1700 against phosphorylated S1700, pS1928 against phosphorylated S1928, and CNC2 against residues 2122-2138 of α 1 1.2, which are ~40 residues upstream of the very C terminus of α 1 1.2.

    Journal: F1000Research

    Article Title: Proteolytic processing of the L-type Ca2+ channel alpha11.2 subunit in neurons

    doi: 10.12688/f1000research.11808.1

    Figure Lengend Snippet: Location of antibody epitopes. Shown is a schematic of the Ca v 1.2 α 1 1.2 subunit, in which regions used as immunogens for the depicted antibodies are identified by arrows. Exact residues are listed in the table and numbered according to α 1 1.2 given in Gene Bank Accession number CAA33546. FP1, CNC1, and ACC-003 are directed against the loop between domains II and III, pS1700 against phosphorylated S1700, pS1928 against phosphorylated S1928, and CNC2 against residues 2122-2138 of α 1 1.2, which are ~40 residues upstream of the very C terminus of α 1 1.2.

    Article Snippet: Concordant with this idea, neither the ACC-003 antibody that we received from Alomone Labs that recognized a 130 kDa band in WT and cKO brains nor our CNC1 antibody recognized the strong 150 kDa band seen with FP1 in brain lysate.

    Techniques:

    Differential recognition of the strong 150 kDa FP1 band in lysate and weak 150 kDa band by FP1, CNC1, and ACC-003 after IP of α 1 1.2 with FP1. Immunoblots with CNC1 ( A , B ), FP1 ( C ), and ACC-003 ( D , E ) of Triton X-100 extracts from WT mice (lysate) and after immunoprecipitation with FP1 from cKO and WT mice. Gels were polymerized from 8% acrylamide. Note that a weak 150 kDa band is detected by CNC1, FP1, and ACC-003 after enrichment of α 1 1.2 by immunoprecipitation with FP1 but the strongly immunoreactive 150 kDa band detected by FP1 in lysate is not detectable by either CNC1 or ACC-003.

    Journal: F1000Research

    Article Title: Proteolytic processing of the L-type Ca2+ channel alpha11.2 subunit in neurons

    doi: 10.12688/f1000research.11808.1

    Figure Lengend Snippet: Differential recognition of the strong 150 kDa FP1 band in lysate and weak 150 kDa band by FP1, CNC1, and ACC-003 after IP of α 1 1.2 with FP1. Immunoblots with CNC1 ( A , B ), FP1 ( C ), and ACC-003 ( D , E ) of Triton X-100 extracts from WT mice (lysate) and after immunoprecipitation with FP1 from cKO and WT mice. Gels were polymerized from 8% acrylamide. Note that a weak 150 kDa band is detected by CNC1, FP1, and ACC-003 after enrichment of α 1 1.2 by immunoprecipitation with FP1 but the strongly immunoreactive 150 kDa band detected by FP1 in lysate is not detectable by either CNC1 or ACC-003.

    Article Snippet: Concordant with this idea, neither the ACC-003 antibody that we received from Alomone Labs that recognized a 130 kDa band in WT and cKO brains nor our CNC1 antibody recognized the strong 150 kDa band seen with FP1 in brain lysate.

    Techniques: Western Blot, Mouse Assay, Immunoprecipitation

    Treatment effects of metformin on the mRNA and protein levels of Cav1.2 in diabetic mice. (A) Relative levels of CACNA1C with metformin in diabetic mice detected by real-time PCR, n=6. (B) Relative levels of Cav1.2 in the Ctrl group, DM group, and the metformin-treatment group detected by western blot, n=6. (C) Representative confocal images of α-Actinin (left) or CaV1.2 (middle) in the Ctrl group, DM group, and the metformin-treatment group. (D) Relative levels of Cav1.2 in the Ctrl group, DM group, and the metformin-treatment group by immunofluorescences, n=9. Values represent mean ± SEM, *P

    Journal: Frontiers in Pharmacology

    Article Title: Metformin Shortens Prolonged QT Interval in Diabetic Mice by Inhibiting L-Type Calcium Current: A Possible Therapeutic Approach

    doi: 10.3389/fphar.2020.00614

    Figure Lengend Snippet: Treatment effects of metformin on the mRNA and protein levels of Cav1.2 in diabetic mice. (A) Relative levels of CACNA1C with metformin in diabetic mice detected by real-time PCR, n=6. (B) Relative levels of Cav1.2 in the Ctrl group, DM group, and the metformin-treatment group detected by western blot, n=6. (C) Representative confocal images of α-Actinin (left) or CaV1.2 (middle) in the Ctrl group, DM group, and the metformin-treatment group. (D) Relative levels of Cav1.2 in the Ctrl group, DM group, and the metformin-treatment group by immunofluorescences, n=9. Values represent mean ± SEM, *P

    Article Snippet: The samples were then incubated at 4°C overnight with primary antibodies for Cav1.2 (Alomone Labs, Jerusalem, Israel; 1:500) and GAPDH (ZSGB, Beijing, China, 1:1000).

    Techniques: Mouse Assay, Real-time Polymerase Chain Reaction, Western Blot

    Treatment effects of metformin on the mRNA and protein levels of Cav1.2 in primary cultured neonatal mice cardiomyocytes. (A) Relative levels of CACNA1C with metformin of primary cardiomyocytes in NC group, HG group and metformin-treatment group detected by real-time PCR, n=6. (B) Relative levels of Cav1.2 of primary cardiomyocytes in NC group, HG group and metformin-treatment group detected by western blot, n=6. (C) Representative confocal images of α-Actinin (left) or CaV1.2 (middle) of primary cardiomyocytes in NC group, HG group and metformin-treatment group. (D) Relative levels of Cav1.2 of primary cardiomyocytes in NC group, HG group and metformin-treatment group detected by immunofluorescences, n=9. Values represent mean ± SEM, *P

    Journal: Frontiers in Pharmacology

    Article Title: Metformin Shortens Prolonged QT Interval in Diabetic Mice by Inhibiting L-Type Calcium Current: A Possible Therapeutic Approach

    doi: 10.3389/fphar.2020.00614

    Figure Lengend Snippet: Treatment effects of metformin on the mRNA and protein levels of Cav1.2 in primary cultured neonatal mice cardiomyocytes. (A) Relative levels of CACNA1C with metformin of primary cardiomyocytes in NC group, HG group and metformin-treatment group detected by real-time PCR, n=6. (B) Relative levels of Cav1.2 of primary cardiomyocytes in NC group, HG group and metformin-treatment group detected by western blot, n=6. (C) Representative confocal images of α-Actinin (left) or CaV1.2 (middle) of primary cardiomyocytes in NC group, HG group and metformin-treatment group. (D) Relative levels of Cav1.2 of primary cardiomyocytes in NC group, HG group and metformin-treatment group detected by immunofluorescences, n=9. Values represent mean ± SEM, *P

    Article Snippet: The samples were then incubated at 4°C overnight with primary antibodies for Cav1.2 (Alomone Labs, Jerusalem, Israel; 1:500) and GAPDH (ZSGB, Beijing, China, 1:1000).

    Techniques: Cell Culture, Mouse Assay, Real-time Polymerase Chain Reaction, Western Blot