Structured Review

Applied Bioinformatics calcium channel cav1 2
Calcium Channel Cav1 2, supplied by Applied Bioinformatics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/calcium channel cav1 2/product/Applied Bioinformatics
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
calcium channel cav1 2 - by Bioz Stars, 2024-07
86/100 stars

Images


Structured Review

Applied Bioinformatics calcium channel cav1 2
Calcium Channel Cav1 2, supplied by Applied Bioinformatics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/calcium channel cav1 2/product/Applied Bioinformatics
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
calcium channel cav1 2 - by Bioz Stars, 2024-07
86/100 stars

Images

cardiac calcium channels cav1 2  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Thermo Fisher cardiac calcium channels cav1 2
    Cardiac Calcium Channels Cav1 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cardiac calcium channels cav1 2/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cardiac calcium channels cav1 2 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    cardiac calcium channels cav1 2  (Thermo Fisher)


    Bioz Verified Symbol Thermo Fisher is a verified supplier
    Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Thermo Fisher cardiac calcium channels cav1 2
    Ion channel protein expression of the ventricular tissue of the PM2.5 group (n = 6) and the control group (n = 5). Panel A showed the relative protein levels of <t>Cav1.2,</t> SERCA2, RYR, NCX, Nav1.5, Kv11.1, KvLQ1 and Kir2.1. Panel B showed the western blot analysis of the ion channel protein expression. * Indicate p < 0.05 comparison of CaV1.2 was performed with Mann–Whitney U Test, while the remaining were performed with Student’s t-test. CaV1.2 = cardiac calcium channels, SERCA2 = SR-calcium-ATPase2, RYR = ryanodine receptor, NCX = sodium/calcium-exchanger, Nav1.5 = cardiac sodium channels Nav1.5, Kv11.1 = voltage-gated potassium channel Kv11.1, KvLQT1 = voltage-gated potassium channel KvLQT1, Kir2.1 = inward-rectifier potassium ion channel.
    Cardiac Calcium Channels Cav1 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cardiac calcium channels cav1 2/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cardiac calcium channels cav1 2 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Neural mechanism facilitating PM2.5-related cardiac arrhythmias through cardiovascular autonomic and calcium dysregulation in a rat model"

    Article Title: Neural mechanism facilitating PM2.5-related cardiac arrhythmias through cardiovascular autonomic and calcium dysregulation in a rat model

    Journal: Scientific Reports

    doi: 10.1038/s41598-023-41148-8

    Ion channel protein expression of the ventricular tissue of the PM2.5 group (n = 6) and the control group (n = 5). Panel A showed the relative protein levels of Cav1.2, SERCA2, RYR, NCX, Nav1.5, Kv11.1, KvLQ1 and Kir2.1. Panel B showed the western blot analysis of the ion channel protein expression. * Indicate p < 0.05 comparison of CaV1.2 was performed with Mann–Whitney U Test, while the remaining were performed with Student’s t-test. CaV1.2 = cardiac calcium channels, SERCA2 = SR-calcium-ATPase2, RYR = ryanodine receptor, NCX = sodium/calcium-exchanger, Nav1.5 = cardiac sodium channels Nav1.5, Kv11.1 = voltage-gated potassium channel Kv11.1, KvLQT1 = voltage-gated potassium channel KvLQT1, Kir2.1 = inward-rectifier potassium ion channel.
    Figure Legend Snippet: Ion channel protein expression of the ventricular tissue of the PM2.5 group (n = 6) and the control group (n = 5). Panel A showed the relative protein levels of Cav1.2, SERCA2, RYR, NCX, Nav1.5, Kv11.1, KvLQ1 and Kir2.1. Panel B showed the western blot analysis of the ion channel protein expression. * Indicate p < 0.05 comparison of CaV1.2 was performed with Mann–Whitney U Test, while the remaining were performed with Student’s t-test. CaV1.2 = cardiac calcium channels, SERCA2 = SR-calcium-ATPase2, RYR = ryanodine receptor, NCX = sodium/calcium-exchanger, Nav1.5 = cardiac sodium channels Nav1.5, Kv11.1 = voltage-gated potassium channel Kv11.1, KvLQT1 = voltage-gated potassium channel KvLQT1, Kir2.1 = inward-rectifier potassium ion channel.

    Techniques Used: Expressing, Western Blot, Comparison, MANN-WHITNEY


    Structured Review

    AUTODOCK GmbH cav1 2 l type voltage gated calcium channel
    Cav1 2 L Type Voltage Gated Calcium Channel, supplied by AUTODOCK GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cav1 2 l type voltage gated calcium channel/product/AUTODOCK GmbH
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cav1 2 l type voltage gated calcium channel - by Bioz Stars, 2024-07
    86/100 stars

    Images

    cardiac calcium channels cav1 2  (Thermo Fisher)


    Bioz Verified Symbol Thermo Fisher is a verified supplier
    Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Thermo Fisher cardiac calcium channels cav1 2
    Cardiac Calcium Channels Cav1 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cardiac calcium channels cav1 2/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cardiac calcium channels cav1 2 - by Bioz Stars, 2024-07
    86/100 stars

    Images


    Structured Review

    AUTODOCK GmbH l type cav1 2 calcium channel cacna1
    L Type Cav1 2 Calcium Channel Cacna1, supplied by AUTODOCK GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l type cav1 2 calcium channel cacna1/product/AUTODOCK GmbH
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l type cav1 2 calcium channel cacna1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    l type calcium channel cav1 2  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Alomone Labs l type calcium channel cav1 2
    L Type Calcium Channel Cav1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l type calcium channel cav1 2/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l type calcium channel cav1 2 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    l type calcium channel cav1 2  (Danaher Inc)


    Bioz Verified Symbol Danaher Inc is a verified supplier
    Bioz Manufacturer Symbol Danaher Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Danaher Inc l type calcium channel cav1 2
    L Type Calcium Channel Cav1 2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l type calcium channel cav1 2/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l type calcium channel cav1 2 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    l type calcium channel mouse monoclonal antibodies against cav1 2  (Antibodies Inc)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Antibodies Inc l type calcium channel mouse monoclonal antibodies against cav1 2
    To determine the influence of <t>LTCC</t> inhibition on aquaporin-0 expression and phosphorylation, control lenses and lenses treated with felodipine (10 µM) for 6 (A) and 24 (B) hrs were examined for changes in the levels of total aquaporin-0 (MIP-26), Ser235-phosphorylated aquaporin-0 (Ser235 Phospho-MIP26) and serine phosphorylated proteins in membrane-enriched lens insoluble fractions, This analysis was performed by immunoblotting using the respective antibodies (see ) and subsequent quantification with Image J based densitometric analysis (Panels C, D and E). The levels of both Ser235 phosphorylated MIP-26 (aquaporin-0) and serine phosphorylated proteins (∼28 kDa) exhibited significant increases (* P<0.01; n = 4) in felodipine treated (24 hrs) specimens compared to control lenses. The levels of total MIP-26 were found to be unaltered by felodipine treatment. Total MIP-26 was used as a loading control for the phospho-MIP26 analysis. Samples derived from three independent lenses were depicted from both the felodipine and DMSO treated groups. Panel F depicts the distribution of MIP-26 (aquaporin-0) in equatorial sections from felodipine and DMSO treated lenses based on immunofluorescence analysis using a MIP-26 polyclonal antibody. Inserts in Panel F show fiber cell swelling in the felodipine treated specimens (indicated with arrows). Bar indicates magnification. Epi: Epithelium, CF: cortical fibers, NF: Nuclear fibers.
    L Type Calcium Channel Mouse Monoclonal Antibodies Against Cav1 2, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l type calcium channel mouse monoclonal antibodies against cav1 2/product/Antibodies Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l type calcium channel mouse monoclonal antibodies against cav1 2 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "L-Type Calcium Channels Play a Critical Role in Maintaining Lens Transparency by Regulating Phosphorylation of Aquaporin-0 and Myosin Light Chain and Expression of Connexins"

    Article Title: L-Type Calcium Channels Play a Critical Role in Maintaining Lens Transparency by Regulating Phosphorylation of Aquaporin-0 and Myosin Light Chain and Expression of Connexins

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064676

    To determine the influence of LTCC inhibition on aquaporin-0 expression and phosphorylation, control lenses and lenses treated with felodipine (10 µM) for 6 (A) and 24 (B) hrs were examined for changes in the levels of total aquaporin-0 (MIP-26), Ser235-phosphorylated aquaporin-0 (Ser235 Phospho-MIP26) and serine phosphorylated proteins in membrane-enriched lens insoluble fractions, This analysis was performed by immunoblotting using the respective antibodies (see ) and subsequent quantification with Image J based densitometric analysis (Panels C, D and E). The levels of both Ser235 phosphorylated MIP-26 (aquaporin-0) and serine phosphorylated proteins (∼28 kDa) exhibited significant increases (* P<0.01; n = 4) in felodipine treated (24 hrs) specimens compared to control lenses. The levels of total MIP-26 were found to be unaltered by felodipine treatment. Total MIP-26 was used as a loading control for the phospho-MIP26 analysis. Samples derived from three independent lenses were depicted from both the felodipine and DMSO treated groups. Panel F depicts the distribution of MIP-26 (aquaporin-0) in equatorial sections from felodipine and DMSO treated lenses based on immunofluorescence analysis using a MIP-26 polyclonal antibody. Inserts in Panel F show fiber cell swelling in the felodipine treated specimens (indicated with arrows). Bar indicates magnification. Epi: Epithelium, CF: cortical fibers, NF: Nuclear fibers.
    Figure Legend Snippet: To determine the influence of LTCC inhibition on aquaporin-0 expression and phosphorylation, control lenses and lenses treated with felodipine (10 µM) for 6 (A) and 24 (B) hrs were examined for changes in the levels of total aquaporin-0 (MIP-26), Ser235-phosphorylated aquaporin-0 (Ser235 Phospho-MIP26) and serine phosphorylated proteins in membrane-enriched lens insoluble fractions, This analysis was performed by immunoblotting using the respective antibodies (see ) and subsequent quantification with Image J based densitometric analysis (Panels C, D and E). The levels of both Ser235 phosphorylated MIP-26 (aquaporin-0) and serine phosphorylated proteins (∼28 kDa) exhibited significant increases (* P<0.01; n = 4) in felodipine treated (24 hrs) specimens compared to control lenses. The levels of total MIP-26 were found to be unaltered by felodipine treatment. Total MIP-26 was used as a loading control for the phospho-MIP26 analysis. Samples derived from three independent lenses were depicted from both the felodipine and DMSO treated groups. Panel F depicts the distribution of MIP-26 (aquaporin-0) in equatorial sections from felodipine and DMSO treated lenses based on immunofluorescence analysis using a MIP-26 polyclonal antibody. Inserts in Panel F show fiber cell swelling in the felodipine treated specimens (indicated with arrows). Bar indicates magnification. Epi: Epithelium, CF: cortical fibers, NF: Nuclear fibers.

    Techniques Used: Inhibition, Expressing, Western Blot, Derivative Assay, Immunofluorescence

    To determine the effects of inhibiting LTCC activity by felodipine (10 µM) on MLC phosphorylation and total MLC levels in the lens, felodipine and DMSO (control) treated mouse lenses were analyzed for changes in the levels of phospho-MLC and total MLC by immunoblot analysis. The levels of phospho-MLC exhibited marked and significant decreases in felodipine treated lenses after 6 hrs (A) and 24 hrs (B) of drug exposure, relative to control lenses. Interestingly, the same samples also exhibited significant increases in the level of total MLC following either a 6 hr or a 24 hr exposure to felodipine, as compared to the corresponding control samples (A & B). Panels C and D show quantitative changes in the levels of phospho-MLC and total MLC respectively in felodipine treated lenses based on densitometric analysis of 4 independent specimens. Lanes 1 to 3 represent three independent samples from both control and drug treated groups.
    Figure Legend Snippet: To determine the effects of inhibiting LTCC activity by felodipine (10 µM) on MLC phosphorylation and total MLC levels in the lens, felodipine and DMSO (control) treated mouse lenses were analyzed for changes in the levels of phospho-MLC and total MLC by immunoblot analysis. The levels of phospho-MLC exhibited marked and significant decreases in felodipine treated lenses after 6 hrs (A) and 24 hrs (B) of drug exposure, relative to control lenses. Interestingly, the same samples also exhibited significant increases in the level of total MLC following either a 6 hr or a 24 hr exposure to felodipine, as compared to the corresponding control samples (A & B). Panels C and D show quantitative changes in the levels of phospho-MLC and total MLC respectively in felodipine treated lenses based on densitometric analysis of 4 independent specimens. Lanes 1 to 3 represent three independent samples from both control and drug treated groups.

    Techniques Used: Activity Assay, Western Blot

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Applied Bioinformatics calcium channel cav1 2
    Calcium Channel Cav1 2, supplied by Applied Bioinformatics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcium channel cav1 2/product/Applied Bioinformatics
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calcium channel cav1 2 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher cardiac calcium channels cav1 2
    Cardiac Calcium Channels Cav1 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cardiac calcium channels cav1 2/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cardiac calcium channels cav1 2 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    AUTODOCK GmbH cav1 2 l type voltage gated calcium channel
    Cav1 2 L Type Voltage Gated Calcium Channel, supplied by AUTODOCK GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cav1 2 l type voltage gated calcium channel/product/AUTODOCK GmbH
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cav1 2 l type voltage gated calcium channel - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    AUTODOCK GmbH l type cav1 2 calcium channel cacna1
    L Type Cav1 2 Calcium Channel Cacna1, supplied by AUTODOCK GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l type cav1 2 calcium channel cacna1/product/AUTODOCK GmbH
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l type cav1 2 calcium channel cacna1 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Alomone Labs l type calcium channel cav1 2
    L Type Calcium Channel Cav1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l type calcium channel cav1 2/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l type calcium channel cav1 2 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Danaher Inc l type calcium channel cav1 2
    L Type Calcium Channel Cav1 2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l type calcium channel cav1 2/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l type calcium channel cav1 2 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Antibodies Inc l type calcium channel mouse monoclonal antibodies against cav1 2
    To determine the influence of <t>LTCC</t> inhibition on aquaporin-0 expression and phosphorylation, control lenses and lenses treated with felodipine (10 µM) for 6 (A) and 24 (B) hrs were examined for changes in the levels of total aquaporin-0 (MIP-26), Ser235-phosphorylated aquaporin-0 (Ser235 Phospho-MIP26) and serine phosphorylated proteins in membrane-enriched lens insoluble fractions, This analysis was performed by immunoblotting using the respective antibodies (see ) and subsequent quantification with Image J based densitometric analysis (Panels C, D and E). The levels of both Ser235 phosphorylated MIP-26 (aquaporin-0) and serine phosphorylated proteins (∼28 kDa) exhibited significant increases (* P<0.01; n = 4) in felodipine treated (24 hrs) specimens compared to control lenses. The levels of total MIP-26 were found to be unaltered by felodipine treatment. Total MIP-26 was used as a loading control for the phospho-MIP26 analysis. Samples derived from three independent lenses were depicted from both the felodipine and DMSO treated groups. Panel F depicts the distribution of MIP-26 (aquaporin-0) in equatorial sections from felodipine and DMSO treated lenses based on immunofluorescence analysis using a MIP-26 polyclonal antibody. Inserts in Panel F show fiber cell swelling in the felodipine treated specimens (indicated with arrows). Bar indicates magnification. Epi: Epithelium, CF: cortical fibers, NF: Nuclear fibers.
    L Type Calcium Channel Mouse Monoclonal Antibodies Against Cav1 2, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l type calcium channel mouse monoclonal antibodies against cav1 2/product/Antibodies Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l type calcium channel mouse monoclonal antibodies against cav1 2 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    To determine the influence of LTCC inhibition on aquaporin-0 expression and phosphorylation, control lenses and lenses treated with felodipine (10 µM) for 6 (A) and 24 (B) hrs were examined for changes in the levels of total aquaporin-0 (MIP-26), Ser235-phosphorylated aquaporin-0 (Ser235 Phospho-MIP26) and serine phosphorylated proteins in membrane-enriched lens insoluble fractions, This analysis was performed by immunoblotting using the respective antibodies (see ) and subsequent quantification with Image J based densitometric analysis (Panels C, D and E). The levels of both Ser235 phosphorylated MIP-26 (aquaporin-0) and serine phosphorylated proteins (∼28 kDa) exhibited significant increases (* P<0.01; n = 4) in felodipine treated (24 hrs) specimens compared to control lenses. The levels of total MIP-26 were found to be unaltered by felodipine treatment. Total MIP-26 was used as a loading control for the phospho-MIP26 analysis. Samples derived from three independent lenses were depicted from both the felodipine and DMSO treated groups. Panel F depicts the distribution of MIP-26 (aquaporin-0) in equatorial sections from felodipine and DMSO treated lenses based on immunofluorescence analysis using a MIP-26 polyclonal antibody. Inserts in Panel F show fiber cell swelling in the felodipine treated specimens (indicated with arrows). Bar indicates magnification. Epi: Epithelium, CF: cortical fibers, NF: Nuclear fibers.

    Journal: PLoS ONE

    Article Title: L-Type Calcium Channels Play a Critical Role in Maintaining Lens Transparency by Regulating Phosphorylation of Aquaporin-0 and Myosin Light Chain and Expression of Connexins

    doi: 10.1371/journal.pone.0064676

    Figure Lengend Snippet: To determine the influence of LTCC inhibition on aquaporin-0 expression and phosphorylation, control lenses and lenses treated with felodipine (10 µM) for 6 (A) and 24 (B) hrs were examined for changes in the levels of total aquaporin-0 (MIP-26), Ser235-phosphorylated aquaporin-0 (Ser235 Phospho-MIP26) and serine phosphorylated proteins in membrane-enriched lens insoluble fractions, This analysis was performed by immunoblotting using the respective antibodies (see ) and subsequent quantification with Image J based densitometric analysis (Panels C, D and E). The levels of both Ser235 phosphorylated MIP-26 (aquaporin-0) and serine phosphorylated proteins (∼28 kDa) exhibited significant increases (* P<0.01; n = 4) in felodipine treated (24 hrs) specimens compared to control lenses. The levels of total MIP-26 were found to be unaltered by felodipine treatment. Total MIP-26 was used as a loading control for the phospho-MIP26 analysis. Samples derived from three independent lenses were depicted from both the felodipine and DMSO treated groups. Panel F depicts the distribution of MIP-26 (aquaporin-0) in equatorial sections from felodipine and DMSO treated lenses based on immunofluorescence analysis using a MIP-26 polyclonal antibody. Inserts in Panel F show fiber cell swelling in the felodipine treated specimens (indicated with arrows). Bar indicates magnification. Epi: Epithelium, CF: cortical fibers, NF: Nuclear fibers.

    Article Snippet: L-type calcium channel mouse monoclonal antibodies against CaV1.2 (C. No. 75-053) and CaV1.3 (C. No. 75-080) were procured from Antibodies Incorporated Davis, CA.

    Techniques: Inhibition, Expressing, Western Blot, Derivative Assay, Immunofluorescence

    To determine the effects of inhibiting LTCC activity by felodipine (10 µM) on MLC phosphorylation and total MLC levels in the lens, felodipine and DMSO (control) treated mouse lenses were analyzed for changes in the levels of phospho-MLC and total MLC by immunoblot analysis. The levels of phospho-MLC exhibited marked and significant decreases in felodipine treated lenses after 6 hrs (A) and 24 hrs (B) of drug exposure, relative to control lenses. Interestingly, the same samples also exhibited significant increases in the level of total MLC following either a 6 hr or a 24 hr exposure to felodipine, as compared to the corresponding control samples (A & B). Panels C and D show quantitative changes in the levels of phospho-MLC and total MLC respectively in felodipine treated lenses based on densitometric analysis of 4 independent specimens. Lanes 1 to 3 represent three independent samples from both control and drug treated groups.

    Journal: PLoS ONE

    Article Title: L-Type Calcium Channels Play a Critical Role in Maintaining Lens Transparency by Regulating Phosphorylation of Aquaporin-0 and Myosin Light Chain and Expression of Connexins

    doi: 10.1371/journal.pone.0064676

    Figure Lengend Snippet: To determine the effects of inhibiting LTCC activity by felodipine (10 µM) on MLC phosphorylation and total MLC levels in the lens, felodipine and DMSO (control) treated mouse lenses were analyzed for changes in the levels of phospho-MLC and total MLC by immunoblot analysis. The levels of phospho-MLC exhibited marked and significant decreases in felodipine treated lenses after 6 hrs (A) and 24 hrs (B) of drug exposure, relative to control lenses. Interestingly, the same samples also exhibited significant increases in the level of total MLC following either a 6 hr or a 24 hr exposure to felodipine, as compared to the corresponding control samples (A & B). Panels C and D show quantitative changes in the levels of phospho-MLC and total MLC respectively in felodipine treated lenses based on densitometric analysis of 4 independent specimens. Lanes 1 to 3 represent three independent samples from both control and drug treated groups.

    Article Snippet: L-type calcium channel mouse monoclonal antibodies against CaV1.2 (C. No. 75-053) and CaV1.3 (C. No. 75-080) were procured from Antibodies Incorporated Davis, CA.

    Techniques: Activity Assay, Western Blot