ca v 1 3  (Alomone Labs)


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    Alomone Labs ca v 1 3
    A , IHC positioned in the middle coil (frequency ∼2 kHz) of the adult P20 gerbil cochlea immunostained for the Ca V 1.3 Ca 2+ channel (red) and ribbon marker CtBP2/RIBEYE (green). Note that both Ca V 1.3 Ca 2+ channels and ribbons are localized at the IHC basal pole, which is the region used for all single Ca 2+ channel recordings shown in the following figures. Merged images are shown in the right column, which show colocalization between CtBP2/RIBEYE and Ca 2+ channel immunopositive spots in yellow. White dotted lines delineate the IHC. Images represent the maximum intensity projection over all layers of the z-stack. Nuclei were stained with DAPI (blue). Scale bar = 10 µm. B , Total number of immunopositive spots for Ca V 1.3 (red bar), for CtBP2/RIBEYE (green bar) and colocalized (yellow bar); n = 13 IHCs analyzed from three gerbils.
    Ca V 1 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Fine Tuning of Ca V 1.3 Ca 2+ Channel Properties in Adult Inner Hair Cells Positioned in the Most Sensitive Region of the Gerbil Cochlea"

    Article Title: Fine Tuning of Ca V 1.3 Ca 2+ Channel Properties in Adult Inner Hair Cells Positioned in the Most Sensitive Region of the Gerbil Cochlea

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0113750

    A , IHC positioned in the middle coil (frequency ∼2 kHz) of the adult P20 gerbil cochlea immunostained for the Ca V 1.3 Ca 2+ channel (red) and ribbon marker CtBP2/RIBEYE (green). Note that both Ca V 1.3 Ca 2+ channels and ribbons are localized at the IHC basal pole, which is the region used for all single Ca 2+ channel recordings shown in the following figures. Merged images are shown in the right column, which show colocalization between CtBP2/RIBEYE and Ca 2+ channel immunopositive spots in yellow. White dotted lines delineate the IHC. Images represent the maximum intensity projection over all layers of the z-stack. Nuclei were stained with DAPI (blue). Scale bar = 10 µm. B , Total number of immunopositive spots for Ca V 1.3 (red bar), for CtBP2/RIBEYE (green bar) and colocalized (yellow bar); n = 13 IHCs analyzed from three gerbils.
    Figure Legend Snippet: A , IHC positioned in the middle coil (frequency ∼2 kHz) of the adult P20 gerbil cochlea immunostained for the Ca V 1.3 Ca 2+ channel (red) and ribbon marker CtBP2/RIBEYE (green). Note that both Ca V 1.3 Ca 2+ channels and ribbons are localized at the IHC basal pole, which is the region used for all single Ca 2+ channel recordings shown in the following figures. Merged images are shown in the right column, which show colocalization between CtBP2/RIBEYE and Ca 2+ channel immunopositive spots in yellow. White dotted lines delineate the IHC. Images represent the maximum intensity projection over all layers of the z-stack. Nuclei were stained with DAPI (blue). Scale bar = 10 µm. B , Total number of immunopositive spots for Ca V 1.3 (red bar), for CtBP2/RIBEYE (green bar) and colocalized (yellow bar); n = 13 IHCs analyzed from three gerbils.

    Techniques Used: Marker, Staining

    ca v 1 3  (Alomone Labs)


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    Alomone Labs ca v 1 3
    A , IHC positioned in the middle coil (frequency ∼2 kHz) of the adult P20 gerbil cochlea immunostained for the Ca V 1.3 Ca 2+ channel (red) and ribbon marker CtBP2/RIBEYE (green). Note that both Ca V 1.3 Ca 2+ channels and ribbons are localized at the IHC basal pole, which is the region used for all single Ca 2+ channel recordings shown in the following figures. Merged images are shown in the right column, which show colocalization between CtBP2/RIBEYE and Ca 2+ channel immunopositive spots in yellow. White dotted lines delineate the IHC. Images represent the maximum intensity projection over all layers of the z-stack. Nuclei were stained with DAPI (blue). Scale bar = 10 µm. B , Total number of immunopositive spots for Ca V 1.3 (red bar), for CtBP2/RIBEYE (green bar) and colocalized (yellow bar); n = 13 IHCs analyzed from three gerbils.
    Ca V 1 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Images

    1) Product Images from "Fine Tuning of Ca V 1.3 Ca 2+ Channel Properties in Adult Inner Hair Cells Positioned in the Most Sensitive Region of the Gerbil Cochlea"

    Article Title: Fine Tuning of Ca V 1.3 Ca 2+ Channel Properties in Adult Inner Hair Cells Positioned in the Most Sensitive Region of the Gerbil Cochlea

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0113750

    A , IHC positioned in the middle coil (frequency ∼2 kHz) of the adult P20 gerbil cochlea immunostained for the Ca V 1.3 Ca 2+ channel (red) and ribbon marker CtBP2/RIBEYE (green). Note that both Ca V 1.3 Ca 2+ channels and ribbons are localized at the IHC basal pole, which is the region used for all single Ca 2+ channel recordings shown in the following figures. Merged images are shown in the right column, which show colocalization between CtBP2/RIBEYE and Ca 2+ channel immunopositive spots in yellow. White dotted lines delineate the IHC. Images represent the maximum intensity projection over all layers of the z-stack. Nuclei were stained with DAPI (blue). Scale bar = 10 µm. B , Total number of immunopositive spots for Ca V 1.3 (red bar), for CtBP2/RIBEYE (green bar) and colocalized (yellow bar); n = 13 IHCs analyzed from three gerbils.
    Figure Legend Snippet: A , IHC positioned in the middle coil (frequency ∼2 kHz) of the adult P20 gerbil cochlea immunostained for the Ca V 1.3 Ca 2+ channel (red) and ribbon marker CtBP2/RIBEYE (green). Note that both Ca V 1.3 Ca 2+ channels and ribbons are localized at the IHC basal pole, which is the region used for all single Ca 2+ channel recordings shown in the following figures. Merged images are shown in the right column, which show colocalization between CtBP2/RIBEYE and Ca 2+ channel immunopositive spots in yellow. White dotted lines delineate the IHC. Images represent the maximum intensity projection over all layers of the z-stack. Nuclei were stained with DAPI (blue). Scale bar = 10 µm. B , Total number of immunopositive spots for Ca V 1.3 (red bar), for CtBP2/RIBEYE (green bar) and colocalized (yellow bar); n = 13 IHCs analyzed from three gerbils.

    Techniques Used: Marker, Staining

    anti ca v 1 3  (Alomone Labs)


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    Alomone Labs anti ca v 1 3
    mRNA expression of L-type calcium channel subunits Ca v 1.2, Ca v 1.3, Ca v 3.1, and Ca v 3.2 (A) (normalized to expression of Tbp) in SAN biopsies from from WT sedentary ( n = 10), WT trained ( n = 10), Girk4 –/– sedentary ( n = 9) and Girk4 –/– trained ( n = 10) mice. Protein expression determined by western blot using antibodies directed against Ca v 1.2 (B) and Ca v 1.3 (C) in individual sinus node biopsies isolated from sedentary WT ( n = 6), trained WT ( n = 4) sedentary Girk4 –/– mice ( n = 6) and trained Girk4 –/– mice ( n = 6). Representative western blots with corresponding stain-free total protein blot used for quantification shown in lower panel. Statistics: Students t -test. * p < 0.05.
    Anti Ca V 1 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti ca v 1 3 - by Bioz Stars, 2023-02
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    Images

    1) Product Images from "Genetic Ablation of G Protein-Gated Inwardly Rectifying K + Channels Prevents Training-Induced Sinus Bradycardia"

    Article Title: Genetic Ablation of G Protein-Gated Inwardly Rectifying K + Channels Prevents Training-Induced Sinus Bradycardia

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2020.519382

    mRNA expression of L-type calcium channel subunits Ca v 1.2, Ca v 1.3, Ca v 3.1, and Ca v 3.2 (A) (normalized to expression of Tbp) in SAN biopsies from from WT sedentary ( n = 10), WT trained ( n = 10), Girk4 –/– sedentary ( n = 9) and Girk4 –/– trained ( n = 10) mice. Protein expression determined by western blot using antibodies directed against Ca v 1.2 (B) and Ca v 1.3 (C) in individual sinus node biopsies isolated from sedentary WT ( n = 6), trained WT ( n = 4) sedentary Girk4 –/– mice ( n = 6) and trained Girk4 –/– mice ( n = 6). Representative western blots with corresponding stain-free total protein blot used for quantification shown in lower panel. Statistics: Students t -test. * p < 0.05.
    Figure Legend Snippet: mRNA expression of L-type calcium channel subunits Ca v 1.2, Ca v 1.3, Ca v 3.1, and Ca v 3.2 (A) (normalized to expression of Tbp) in SAN biopsies from from WT sedentary ( n = 10), WT trained ( n = 10), Girk4 –/– sedentary ( n = 9) and Girk4 –/– trained ( n = 10) mice. Protein expression determined by western blot using antibodies directed against Ca v 1.2 (B) and Ca v 1.3 (C) in individual sinus node biopsies isolated from sedentary WT ( n = 6), trained WT ( n = 4) sedentary Girk4 –/– mice ( n = 6) and trained Girk4 –/– mice ( n = 6). Representative western blots with corresponding stain-free total protein blot used for quantification shown in lower panel. Statistics: Students t -test. * p < 0.05.

    Techniques Used: Expressing, Western Blot, Isolation, Staining

    ca v 1 3  (Alomone Labs)


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    Alomone Labs ca v 1 3
    ALPL deficiency caused decreased membrane expression of L-type Ca 2+ channels in BMSCs. a Ca 2+ imaging showed decreased Ca 2+ influx in cultured alpl +/− BMSCs and WT BMSCs transfected with shALP (shALP/WT) after they were stimulated with 30 mmol·L −1 KCl for 3 min ( n = 10). b No KCl-induced Ca 2+ influx was detected in cultured WT, alpl +/− , and shALP/WT BMSCs treated with 10 mmol·L −1 EGTA for 3 min ( n = 10). c ALPL overexpression was mediated by a lentivirus in alpl +/− (Lenti-alp/ alpl +/− ) BMSCs and resulted in an elevated Ca 2+ influx following stimulation with 30 mmol·L −1 KCl for 3 min ( n = 10). d , e The expression of Ca V 1.1, Ca V 1.2, and Ca V 1.3 was assessed. alpl +/− BMSCs showed decreases in total cell expression ( d ) and membrane expression of Ca V 1.2 and Ca V 1.3 ( e ) and no significant change in the levels of cytoplasmic Ca V 1.2 and Ca V 1.3 ( e ). Total Ca V 1.1 protein expression was not changed ( d ), and the expression of membrane and cytoplasmic Ca V 1.1 was not altered in alpl +/− BMSCs ( e ). f Cell-surface biotinylation assay. Left two lanes: western blot for Ca V 1.2 and Ca V 1.3 following neutravidin pull down from WT and alpl +/− BMSCs; right two lanes: input, not biotinylated cells. g Lenti-alp/ alpl +/− BMSCs showed elevated membrane expression of ALP, Ca V 1.2, and Ca V 1.3. h , i Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 (green) in WT and Lenti-alp/ alpl +/− BMSCs. The plasma membrane was stained with the marker CellMask™ Deep Red Plasma Membrane Stain (red) ( h ). Quantification of the membrane florescence was performed with NIH ImageJ ( i ). Scale bar, 10 μm. The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01; *** P < 0.001
    Ca V 1 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ca v 1 3/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Images

    1) Product Images from "Ionomycin ameliorates hypophosphatasia via rescuing alkaline phosphatase deficiency-mediated L-type Ca 2+ channel internalization in mesenchymal stem cells"

    Article Title: Ionomycin ameliorates hypophosphatasia via rescuing alkaline phosphatase deficiency-mediated L-type Ca 2+ channel internalization in mesenchymal stem cells

    Journal: Bone Research

    doi: 10.1038/s41413-020-0090-7

    ALPL deficiency caused decreased membrane expression of L-type Ca 2+ channels in BMSCs. a Ca 2+ imaging showed decreased Ca 2+ influx in cultured alpl +/− BMSCs and WT BMSCs transfected with shALP (shALP/WT) after they were stimulated with 30 mmol·L −1 KCl for 3 min ( n = 10). b No KCl-induced Ca 2+ influx was detected in cultured WT, alpl +/− , and shALP/WT BMSCs treated with 10 mmol·L −1 EGTA for 3 min ( n = 10). c ALPL overexpression was mediated by a lentivirus in alpl +/− (Lenti-alp/ alpl +/− ) BMSCs and resulted in an elevated Ca 2+ influx following stimulation with 30 mmol·L −1 KCl for 3 min ( n = 10). d , e The expression of Ca V 1.1, Ca V 1.2, and Ca V 1.3 was assessed. alpl +/− BMSCs showed decreases in total cell expression ( d ) and membrane expression of Ca V 1.2 and Ca V 1.3 ( e ) and no significant change in the levels of cytoplasmic Ca V 1.2 and Ca V 1.3 ( e ). Total Ca V 1.1 protein expression was not changed ( d ), and the expression of membrane and cytoplasmic Ca V 1.1 was not altered in alpl +/− BMSCs ( e ). f Cell-surface biotinylation assay. Left two lanes: western blot for Ca V 1.2 and Ca V 1.3 following neutravidin pull down from WT and alpl +/− BMSCs; right two lanes: input, not biotinylated cells. g Lenti-alp/ alpl +/− BMSCs showed elevated membrane expression of ALP, Ca V 1.2, and Ca V 1.3. h , i Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 (green) in WT and Lenti-alp/ alpl +/− BMSCs. The plasma membrane was stained with the marker CellMask™ Deep Red Plasma Membrane Stain (red) ( h ). Quantification of the membrane florescence was performed with NIH ImageJ ( i ). Scale bar, 10 μm. The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01; *** P < 0.001
    Figure Legend Snippet: ALPL deficiency caused decreased membrane expression of L-type Ca 2+ channels in BMSCs. a Ca 2+ imaging showed decreased Ca 2+ influx in cultured alpl +/− BMSCs and WT BMSCs transfected with shALP (shALP/WT) after they were stimulated with 30 mmol·L −1 KCl for 3 min ( n = 10). b No KCl-induced Ca 2+ influx was detected in cultured WT, alpl +/− , and shALP/WT BMSCs treated with 10 mmol·L −1 EGTA for 3 min ( n = 10). c ALPL overexpression was mediated by a lentivirus in alpl +/− (Lenti-alp/ alpl +/− ) BMSCs and resulted in an elevated Ca 2+ influx following stimulation with 30 mmol·L −1 KCl for 3 min ( n = 10). d , e The expression of Ca V 1.1, Ca V 1.2, and Ca V 1.3 was assessed. alpl +/− BMSCs showed decreases in total cell expression ( d ) and membrane expression of Ca V 1.2 and Ca V 1.3 ( e ) and no significant change in the levels of cytoplasmic Ca V 1.2 and Ca V 1.3 ( e ). Total Ca V 1.1 protein expression was not changed ( d ), and the expression of membrane and cytoplasmic Ca V 1.1 was not altered in alpl +/− BMSCs ( e ). f Cell-surface biotinylation assay. Left two lanes: western blot for Ca V 1.2 and Ca V 1.3 following neutravidin pull down from WT and alpl +/− BMSCs; right two lanes: input, not biotinylated cells. g Lenti-alp/ alpl +/− BMSCs showed elevated membrane expression of ALP, Ca V 1.2, and Ca V 1.3. h , i Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 (green) in WT and Lenti-alp/ alpl +/− BMSCs. The plasma membrane was stained with the marker CellMask™ Deep Red Plasma Membrane Stain (red) ( h ). Quantification of the membrane florescence was performed with NIH ImageJ ( i ). Scale bar, 10 μm. The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01; *** P < 0.001

    Techniques Used: Expressing, Imaging, Cell Culture, Transfection, Over Expression, Cell Surface Biotinylation Assay, Western Blot, Confocal Laser Scanning Microscopy, Staining, Marker

    ALPL-maintained MSC osteogenic/adipogenic lineage differentiation ability via the L-type Ca 2+ channel. a Ca 2+ imaging showed elevated Ca 2+ influx in alpl +/− BMSCs transfected with oeCa V 1.2 or oeCa V 1.3 following stimulation with 30 mmol·L −1 KCl for 3 min ( n = 10). b , c Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 (green) in alpl +/− BMSCs transfected with oeCa V 1.2 or oeCa V 1.3. The plasma membrane was stained with the marker CellMask™ Deep Red Plasma Membrane Stain (red) ( b ). Quantification of the membrane florescence was performed with NIH ImageJ ( c ). Scale bar, 10 μm. Alizarin red staining showed that alpl +/− BMSCs transfected with oeCa V 1.2 or oeCa V 1.3 had an increased capacity to form mineralized nodules when cultured under osteoinductive conditions ( d ) and they exhibited an upregulation of the osteogenic-related proteins RUNX2 and Sp7 ( e ). oeCa V 1.2- or oeCa V 1.3-treated alpl +/− BMSCs showed a decreased number of oil red O-positive adipocytes when cultured under adipo-inductive conditions ( f ) and there was a downregulation of the adipogenic-related proteins PPARγ2 and LPL, as assessed by western blot ( g ). h Alizarin red staining showed that alpl +/− BMSCs transfected with siCa V 1.2 or siCa V 1.3 had a decreased capacity to form mineralized nodules when cultured under osteoinductive conditions. i Western blot analysis showed that BMSCs transfected with siCa V 1.2 or siCa V 1.3 expressed decreased levels of the osteogenic-related proteins RUNX2 and Sp7. β-actin was used as a protein loading control. BMSCs transfected with siCa V 1.2 or siCa V 1.3 showed an increased number of oil red O-positive adipocytes when cultured under adipo-inductive conditions ( j ) and upregulation of the adipogenic-related proteins PPARγ2 and LPL, as assessed by western blot ( k ). The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01; *** P < 0.001
    Figure Legend Snippet: ALPL-maintained MSC osteogenic/adipogenic lineage differentiation ability via the L-type Ca 2+ channel. a Ca 2+ imaging showed elevated Ca 2+ influx in alpl +/− BMSCs transfected with oeCa V 1.2 or oeCa V 1.3 following stimulation with 30 mmol·L −1 KCl for 3 min ( n = 10). b , c Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 (green) in alpl +/− BMSCs transfected with oeCa V 1.2 or oeCa V 1.3. The plasma membrane was stained with the marker CellMask™ Deep Red Plasma Membrane Stain (red) ( b ). Quantification of the membrane florescence was performed with NIH ImageJ ( c ). Scale bar, 10 μm. Alizarin red staining showed that alpl +/− BMSCs transfected with oeCa V 1.2 or oeCa V 1.3 had an increased capacity to form mineralized nodules when cultured under osteoinductive conditions ( d ) and they exhibited an upregulation of the osteogenic-related proteins RUNX2 and Sp7 ( e ). oeCa V 1.2- or oeCa V 1.3-treated alpl +/− BMSCs showed a decreased number of oil red O-positive adipocytes when cultured under adipo-inductive conditions ( f ) and there was a downregulation of the adipogenic-related proteins PPARγ2 and LPL, as assessed by western blot ( g ). h Alizarin red staining showed that alpl +/− BMSCs transfected with siCa V 1.2 or siCa V 1.3 had a decreased capacity to form mineralized nodules when cultured under osteoinductive conditions. i Western blot analysis showed that BMSCs transfected with siCa V 1.2 or siCa V 1.3 expressed decreased levels of the osteogenic-related proteins RUNX2 and Sp7. β-actin was used as a protein loading control. BMSCs transfected with siCa V 1.2 or siCa V 1.3 showed an increased number of oil red O-positive adipocytes when cultured under adipo-inductive conditions ( j ) and upregulation of the adipogenic-related proteins PPARγ2 and LPL, as assessed by western blot ( k ). The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01; *** P < 0.001

    Techniques Used: Imaging, Transfection, Confocal Laser Scanning Microscopy, Staining, Marker, Cell Culture, Western Blot

    ALPL deficiency promoted the internalization of L-type Ca 2+ channels in BMSCs. a , b Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 (green) in alpl +/− BMSCs transfected with DN-Dyn1. The plasma membrane was stained with the marker CellMask™ Deep Red Plasma Membrane Stain (red) ( a ). Quantification of the membrane florescence was performed with NIH ImageJ ( b ). Scale bar, 10 μm. c alpl +/− BMSCs transfected with DN-Dyn1 showed upregulation of Ca V 1.2 and Ca V 1.3 membrane expression and almost no change in the cytoplasmic expression of Ca V 1.2 and Ca V 1.3, as assessed by western blot. β-actin was used as a protein loading control. d 10-min time-lapse confocal laser scanning microscopy images of WT, alpl +/− , DN-Dyn1/ alpl +/− , and Lenti-alp/ alpl +/− BMSCs containing DsRed-CaV1.2. Scale bar, 10 μm. e Ca 2+ imaging showed elevated Ca 2+ influx of cultured alpl +/− BMSCs transfected with DN-Dyn1 after stimulation with 30 mmol·L −1 KCl for 3 min ( n = 10). alpl +/− BMSCs showed a pronounced decrease in DsRed-Ca V 1.2 at the membrane (Dio-labeled ROI, n = 10) ( f ). g Quantification of the florescence in the ROI during the time-course lapse at 0 s, 300 s, and 600 s. h – k Representative images show the colocalization of DsRed-Ca V 1.2 with the Dio-labeled cell membrane of BMSCs. WT, DN-Dyn1/ alpl +/− , and Lenti-alp/ alpl +/− BMSCs had colocalized regions at 0 s and 570 s ( h , j , k ). However, alpl +/− BMSCs showed no colocalization at 0 s and 570 s ( i ). The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01
    Figure Legend Snippet: ALPL deficiency promoted the internalization of L-type Ca 2+ channels in BMSCs. a , b Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 (green) in alpl +/− BMSCs transfected with DN-Dyn1. The plasma membrane was stained with the marker CellMask™ Deep Red Plasma Membrane Stain (red) ( a ). Quantification of the membrane florescence was performed with NIH ImageJ ( b ). Scale bar, 10 μm. c alpl +/− BMSCs transfected with DN-Dyn1 showed upregulation of Ca V 1.2 and Ca V 1.3 membrane expression and almost no change in the cytoplasmic expression of Ca V 1.2 and Ca V 1.3, as assessed by western blot. β-actin was used as a protein loading control. d 10-min time-lapse confocal laser scanning microscopy images of WT, alpl +/− , DN-Dyn1/ alpl +/− , and Lenti-alp/ alpl +/− BMSCs containing DsRed-CaV1.2. Scale bar, 10 μm. e Ca 2+ imaging showed elevated Ca 2+ influx of cultured alpl +/− BMSCs transfected with DN-Dyn1 after stimulation with 30 mmol·L −1 KCl for 3 min ( n = 10). alpl +/− BMSCs showed a pronounced decrease in DsRed-Ca V 1.2 at the membrane (Dio-labeled ROI, n = 10) ( f ). g Quantification of the florescence in the ROI during the time-course lapse at 0 s, 300 s, and 600 s. h – k Representative images show the colocalization of DsRed-Ca V 1.2 with the Dio-labeled cell membrane of BMSCs. WT, DN-Dyn1/ alpl +/− , and Lenti-alp/ alpl +/− BMSCs had colocalized regions at 0 s and 570 s ( h , j , k ). However, alpl +/− BMSCs showed no colocalization at 0 s and 570 s ( i ). The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01

    Techniques Used: Confocal Laser Scanning Microscopy, Transfection, Staining, Marker, Expressing, Western Blot, Imaging, Cell Culture, Labeling

    ALPL deficiency promoted the internalization of L-type Ca 2+ channels via binding to α2δ subunits. a Representative images of confocal laser scanning microscopy showing a region of membrane colocalization for ALPL (DsRed) and Ca V 1.2 (FITC-labeled) or Ca V 1.3 (FITC-labeled) in WT BMSCs. No region of membrane colocalization was found in alpl +/− BMSCs. Scale bar, 10 μm. b ALPL immunoprecipitated Ca V 1.2 and Ca V 1.3. The left lane shows the expression of Ca V 1.2 and Ca V 1.3, and the right lane shows the levels of Ca V 1.2 and Ca V 1.3 following immunoprecipitation with an anti-ALPL antibody. c Ca V 1.2 and Ca V 1.3 immunoprecipitated ALPL. The left panel shows the expression of ALPL, and the right panel shows the level of ALPL following immunoprecipitation with anti-Ca V 1.2 or anti-Ca V 1.3 antibodies. d Representative images of confocal laser scanning microscopy showing the membrane colocalization region of ALPL (Cy3-labeled) and Ca V 1.2 (FITC-labeled) or Ca V 1.3 (FITC-labeled) in alpl −/− BMSCs overexpressing ALPL and the α2δ subunit. No membrane colocalization region was found in alpl −/− BMSCs and alpl −/− BMSCs overexpressing ALPL or the mutant α2δ subunit. Scale bar, 10 μm. e Western blot analysis showed membrane expression of Ca V 1.2 or Ca V 1.3 in alpl −/− BMSCs overexpressing ALPL and the α2δ subunit. No membrane expression of Ca V 1.2 or Ca V 1.3 was found in alpl −/− BMSCs and alpl −/− BMSCs overexpressing ALPL or the mutant α2δ subunit. No significant change in cytoplasmic Ca V 1.2 or Ca V 1.3 was found in alpl −/− BMSCs, alpl −/− BMSCs overexpressing ALPL and the mutant α2δ subunit, or alpl −/− BMSCs overexpressing ALPL and the α2δ subunit. f α2δ immunoprecipitated ALPL. The left panel shows the expression of ALPL, and the right panel shows the level of ALPL following immunoprecipitation with an anti-α2δ antibody in alp/ f/f and alpl −/− BMSCs. β-actin was used as a protein loading control. The representative results from three independent experiments are shown
    Figure Legend Snippet: ALPL deficiency promoted the internalization of L-type Ca 2+ channels via binding to α2δ subunits. a Representative images of confocal laser scanning microscopy showing a region of membrane colocalization for ALPL (DsRed) and Ca V 1.2 (FITC-labeled) or Ca V 1.3 (FITC-labeled) in WT BMSCs. No region of membrane colocalization was found in alpl +/− BMSCs. Scale bar, 10 μm. b ALPL immunoprecipitated Ca V 1.2 and Ca V 1.3. The left lane shows the expression of Ca V 1.2 and Ca V 1.3, and the right lane shows the levels of Ca V 1.2 and Ca V 1.3 following immunoprecipitation with an anti-ALPL antibody. c Ca V 1.2 and Ca V 1.3 immunoprecipitated ALPL. The left panel shows the expression of ALPL, and the right panel shows the level of ALPL following immunoprecipitation with anti-Ca V 1.2 or anti-Ca V 1.3 antibodies. d Representative images of confocal laser scanning microscopy showing the membrane colocalization region of ALPL (Cy3-labeled) and Ca V 1.2 (FITC-labeled) or Ca V 1.3 (FITC-labeled) in alpl −/− BMSCs overexpressing ALPL and the α2δ subunit. No membrane colocalization region was found in alpl −/− BMSCs and alpl −/− BMSCs overexpressing ALPL or the mutant α2δ subunit. Scale bar, 10 μm. e Western blot analysis showed membrane expression of Ca V 1.2 or Ca V 1.3 in alpl −/− BMSCs overexpressing ALPL and the α2δ subunit. No membrane expression of Ca V 1.2 or Ca V 1.3 was found in alpl −/− BMSCs and alpl −/− BMSCs overexpressing ALPL or the mutant α2δ subunit. No significant change in cytoplasmic Ca V 1.2 or Ca V 1.3 was found in alpl −/− BMSCs, alpl −/− BMSCs overexpressing ALPL and the mutant α2δ subunit, or alpl −/− BMSCs overexpressing ALPL and the α2δ subunit. f α2δ immunoprecipitated ALPL. The left panel shows the expression of ALPL, and the right panel shows the level of ALPL following immunoprecipitation with an anti-α2δ antibody in alp/ f/f and alpl −/− BMSCs. β-actin was used as a protein loading control. The representative results from three independent experiments are shown

    Techniques Used: Binding Assay, Confocal Laser Scanning Microscopy, Labeling, Immunoprecipitation, Expressing, Mutagenesis, Western Blot

    ALPL deficiency promoted the internalization of L-type Ca 2+ channels in HPP patient-derived BMSCs. a The expression of ALPL on the membrane and cytoplasm was decreased in BMSCs from two HPP patients compared with that of normal human BMSCs. β-actin was used as a protein loading control. b Intracellular Ca 2+ imaging analysis showed that KCl-induced Ca 2+ influx was significantly decreased in cultured BMSCs from HPP patients compared with that of normal human BMSCs. c Overexpression of ALPL or transfection with DN-Dyn1 in BMSCs from A1 patients showed elevated KCl-induced Ca 2+ influx. d Overexpression of ALPL or transfection with DN-Dyn1 in BMSCs from A2 patients showed elevated KCl-induced Ca 2+ influx. e Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 in control BMSCs, A1 BMSCs, A2 BMSCs, A1 and A2 BMSCs overexpressing ALPL, and A1 and A2 BMSCs transfected with DN-Dyn1. Scale bar, 10 μm. The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01
    Figure Legend Snippet: ALPL deficiency promoted the internalization of L-type Ca 2+ channels in HPP patient-derived BMSCs. a The expression of ALPL on the membrane and cytoplasm was decreased in BMSCs from two HPP patients compared with that of normal human BMSCs. β-actin was used as a protein loading control. b Intracellular Ca 2+ imaging analysis showed that KCl-induced Ca 2+ influx was significantly decreased in cultured BMSCs from HPP patients compared with that of normal human BMSCs. c Overexpression of ALPL or transfection with DN-Dyn1 in BMSCs from A1 patients showed elevated KCl-induced Ca 2+ influx. d Overexpression of ALPL or transfection with DN-Dyn1 in BMSCs from A2 patients showed elevated KCl-induced Ca 2+ influx. e Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 in control BMSCs, A1 BMSCs, A2 BMSCs, A1 and A2 BMSCs overexpressing ALPL, and A1 and A2 BMSCs transfected with DN-Dyn1. Scale bar, 10 μm. The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01

    Techniques Used: Derivative Assay, Expressing, Imaging, Cell Culture, Over Expression, Transfection, Confocal Laser Scanning Microscopy

    ca v 3 1  (Alomone Labs)


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    Alomone Labs ca v 3 1
    Ca V 3 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti cav 3 1 antibody  (Alomone Labs)


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    Alomone Labs rabbit anti cav 3 1 antibody
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    anti ca v 3 1 ca v 3 3 antibodies  (Alomone Labs)


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    Alomone Labs anti ca v 3 1 ca v 3 3 antibodies
    Expression of Ca v 3.1-3.3 isoforms (Alomone) in SDH and DRG of SD rats with or without AR pre-treatment. Representative confocal images of Ca v 3.1 (A), Ca v 3.2 (B), and Ca v 3.3 (C) in SDH without AR pre-treatment (No AR). Representative confocal images of Ca v 3.1 (D), Ca v 3.2 (F), and Ca v 3.3 (F) in SDH with AR pre-treatment (AR). Representative images of Ca v 3.1 (G), Ca v 3.2 (H), and Ca v 3.3 (I) in DRG without AR pre-treatment (No AR). Representative images of Ca v 3.1 (J), Ca v 3.2 (K), and Ca v 3.3 (L) in DRG with AR pre-treatment (AR).
    Anti Ca V 3 1 Ca V 3 3 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Antigen retrieval pre-treatment causes a different expression pattern of Cav3.2 in rat and mouse spinal dorsal horn"

    Article Title: Antigen retrieval pre-treatment causes a different expression pattern of Cav3.2 in rat and mouse spinal dorsal horn

    Journal: European Journal of Histochemistry : EJH

    doi: 10.4081/ejh.2019.2988

    Expression of Ca v 3.1-3.3 isoforms (Alomone) in SDH and DRG of SD rats with or without AR pre-treatment. Representative confocal images of Ca v 3.1 (A), Ca v 3.2 (B), and Ca v 3.3 (C) in SDH without AR pre-treatment (No AR). Representative confocal images of Ca v 3.1 (D), Ca v 3.2 (F), and Ca v 3.3 (F) in SDH with AR pre-treatment (AR). Representative images of Ca v 3.1 (G), Ca v 3.2 (H), and Ca v 3.3 (I) in DRG without AR pre-treatment (No AR). Representative images of Ca v 3.1 (J), Ca v 3.2 (K), and Ca v 3.3 (L) in DRG with AR pre-treatment (AR).
    Figure Legend Snippet: Expression of Ca v 3.1-3.3 isoforms (Alomone) in SDH and DRG of SD rats with or without AR pre-treatment. Representative confocal images of Ca v 3.1 (A), Ca v 3.2 (B), and Ca v 3.3 (C) in SDH without AR pre-treatment (No AR). Representative confocal images of Ca v 3.1 (D), Ca v 3.2 (F), and Ca v 3.3 (F) in SDH with AR pre-treatment (AR). Representative images of Ca v 3.1 (G), Ca v 3.2 (H), and Ca v 3.3 (I) in DRG without AR pre-treatment (No AR). Representative images of Ca v 3.1 (J), Ca v 3.2 (K), and Ca v 3.3 (L) in DRG with AR pre-treatment (AR).

    Techniques Used: Expressing

    Expression of Ca v 3.1-3.3 isoforms (Alomone) in mice SDH sections pre-treated with AR or not. Representative images of Ca v 3.1 (A), Ca v 3.2 (B), and Ca v 3.3 (C) in SDH without AR pre-treatment (no AR). Representative images of Ca v 3.1 (D), Ca v 3.2 (E), and Ca v 3.3 (F) in SDH with AR pre-treatment (AR).
    Figure Legend Snippet: Expression of Ca v 3.1-3.3 isoforms (Alomone) in mice SDH sections pre-treated with AR or not. Representative images of Ca v 3.1 (A), Ca v 3.2 (B), and Ca v 3.3 (C) in SDH without AR pre-treatment (no AR). Representative images of Ca v 3.1 (D), Ca v 3.2 (E), and Ca v 3.3 (F) in SDH with AR pre-treatment (AR).

    Techniques Used: Expressing

    ca v 3 1  (Alomone Labs)


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    rabbit anti cav 3 1  (Alomone Labs)


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    rabbit anti cav 3 1  (Alomone Labs)


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    Alomone Labs ca v 1 3
    A , IHC positioned in the middle coil (frequency ∼2 kHz) of the adult P20 gerbil cochlea immunostained for the Ca V 1.3 Ca 2+ channel (red) and ribbon marker CtBP2/RIBEYE (green). Note that both Ca V 1.3 Ca 2+ channels and ribbons are localized at the IHC basal pole, which is the region used for all single Ca 2+ channel recordings shown in the following figures. Merged images are shown in the right column, which show colocalization between CtBP2/RIBEYE and Ca 2+ channel immunopositive spots in yellow. White dotted lines delineate the IHC. Images represent the maximum intensity projection over all layers of the z-stack. Nuclei were stained with DAPI (blue). Scale bar = 10 µm. B , Total number of immunopositive spots for Ca V 1.3 (red bar), for CtBP2/RIBEYE (green bar) and colocalized (yellow bar); n = 13 IHCs analyzed from three gerbils.
    Ca V 1 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs anti ca v 1 3
    mRNA expression of L-type calcium channel subunits Ca v 1.2, Ca v 1.3, Ca v 3.1, and Ca v 3.2 (A) (normalized to expression of Tbp) in SAN biopsies from from WT sedentary ( n = 10), WT trained ( n = 10), Girk4 –/– sedentary ( n = 9) and Girk4 –/– trained ( n = 10) mice. Protein expression determined by western blot using antibodies directed against Ca v 1.2 (B) and Ca v 1.3 (C) in individual sinus node biopsies isolated from sedentary WT ( n = 6), trained WT ( n = 4) sedentary Girk4 –/– mice ( n = 6) and trained Girk4 –/– mice ( n = 6). Representative western blots with corresponding stain-free total protein blot used for quantification shown in lower panel. Statistics: Students t -test. * p < 0.05.
    Anti Ca V 1 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs ca v 3 1
    mRNA expression of L-type calcium channel subunits Ca v 1.2, Ca v 1.3, Ca v 3.1, and Ca v 3.2 (A) (normalized to expression of Tbp) in SAN biopsies from from WT sedentary ( n = 10), WT trained ( n = 10), Girk4 –/– sedentary ( n = 9) and Girk4 –/– trained ( n = 10) mice. Protein expression determined by western blot using antibodies directed against Ca v 1.2 (B) and Ca v 1.3 (C) in individual sinus node biopsies isolated from sedentary WT ( n = 6), trained WT ( n = 4) sedentary Girk4 –/– mice ( n = 6) and trained Girk4 –/– mice ( n = 6). Representative western blots with corresponding stain-free total protein blot used for quantification shown in lower panel. Statistics: Students t -test. * p < 0.05.
    Ca V 3 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ca v 3 1/product/Alomone Labs
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    Alomone Labs rabbit anti cav 3 1 antibody
    mRNA expression of L-type calcium channel subunits Ca v 1.2, Ca v 1.3, Ca v 3.1, and Ca v 3.2 (A) (normalized to expression of Tbp) in SAN biopsies from from WT sedentary ( n = 10), WT trained ( n = 10), Girk4 –/– sedentary ( n = 9) and Girk4 –/– trained ( n = 10) mice. Protein expression determined by western blot using antibodies directed against Ca v 1.2 (B) and Ca v 1.3 (C) in individual sinus node biopsies isolated from sedentary WT ( n = 6), trained WT ( n = 4) sedentary Girk4 –/– mice ( n = 6) and trained Girk4 –/– mice ( n = 6). Representative western blots with corresponding stain-free total protein blot used for quantification shown in lower panel. Statistics: Students t -test. * p < 0.05.
    Rabbit Anti Cav 3 1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cav 3 1 antibody/product/Alomone Labs
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    Alomone Labs anti ca v 3 1 ca v 3 3 antibodies
    Expression of Ca v 3.1-3.3 isoforms (Alomone) in SDH and DRG of SD rats with or without AR pre-treatment. Representative confocal images of Ca v 3.1 (A), Ca v 3.2 (B), and Ca v 3.3 (C) in SDH without AR pre-treatment (No AR). Representative confocal images of Ca v 3.1 (D), Ca v 3.2 (F), and Ca v 3.3 (F) in SDH with AR pre-treatment (AR). Representative images of Ca v 3.1 (G), Ca v 3.2 (H), and Ca v 3.3 (I) in DRG without AR pre-treatment (No AR). Representative images of Ca v 3.1 (J), Ca v 3.2 (K), and Ca v 3.3 (L) in DRG with AR pre-treatment (AR).
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    Alomone Labs rabbit anti cav 3 1
    Expression of Ca v 3.1-3.3 isoforms (Alomone) in SDH and DRG of SD rats with or without AR pre-treatment. Representative confocal images of Ca v 3.1 (A), Ca v 3.2 (B), and Ca v 3.3 (C) in SDH without AR pre-treatment (No AR). Representative confocal images of Ca v 3.1 (D), Ca v 3.2 (F), and Ca v 3.3 (F) in SDH with AR pre-treatment (AR). Representative images of Ca v 3.1 (G), Ca v 3.2 (H), and Ca v 3.3 (I) in DRG without AR pre-treatment (No AR). Representative images of Ca v 3.1 (J), Ca v 3.2 (K), and Ca v 3.3 (L) in DRG with AR pre-treatment (AR).
    Rabbit Anti Cav 3 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A , IHC positioned in the middle coil (frequency ∼2 kHz) of the adult P20 gerbil cochlea immunostained for the Ca V 1.3 Ca 2+ channel (red) and ribbon marker CtBP2/RIBEYE (green). Note that both Ca V 1.3 Ca 2+ channels and ribbons are localized at the IHC basal pole, which is the region used for all single Ca 2+ channel recordings shown in the following figures. Merged images are shown in the right column, which show colocalization between CtBP2/RIBEYE and Ca 2+ channel immunopositive spots in yellow. White dotted lines delineate the IHC. Images represent the maximum intensity projection over all layers of the z-stack. Nuclei were stained with DAPI (blue). Scale bar = 10 µm. B , Total number of immunopositive spots for Ca V 1.3 (red bar), for CtBP2/RIBEYE (green bar) and colocalized (yellow bar); n = 13 IHCs analyzed from three gerbils.

    Journal: PLoS ONE

    Article Title: Fine Tuning of Ca V 1.3 Ca 2+ Channel Properties in Adult Inner Hair Cells Positioned in the Most Sensitive Region of the Gerbil Cochlea

    doi: 10.1371/journal.pone.0113750

    Figure Lengend Snippet: A , IHC positioned in the middle coil (frequency ∼2 kHz) of the adult P20 gerbil cochlea immunostained for the Ca V 1.3 Ca 2+ channel (red) and ribbon marker CtBP2/RIBEYE (green). Note that both Ca V 1.3 Ca 2+ channels and ribbons are localized at the IHC basal pole, which is the region used for all single Ca 2+ channel recordings shown in the following figures. Merged images are shown in the right column, which show colocalization between CtBP2/RIBEYE and Ca 2+ channel immunopositive spots in yellow. White dotted lines delineate the IHC. Images represent the maximum intensity projection over all layers of the z-stack. Nuclei were stained with DAPI (blue). Scale bar = 10 µm. B , Total number of immunopositive spots for Ca V 1.3 (red bar), for CtBP2/RIBEYE (green bar) and colocalized (yellow bar); n = 13 IHCs analyzed from three gerbils.

    Article Snippet: Antibodies directed against Ca V 1.3 (rabbit, Alomone Laboratories, diluted 1∶50) and Ribeye/CtBP2 (mouse, BD Transduction Laboratories, diluted 1∶50) were used for cryosection preparations.

    Techniques: Marker, Staining

    mRNA expression of L-type calcium channel subunits Ca v 1.2, Ca v 1.3, Ca v 3.1, and Ca v 3.2 (A) (normalized to expression of Tbp) in SAN biopsies from from WT sedentary ( n = 10), WT trained ( n = 10), Girk4 –/– sedentary ( n = 9) and Girk4 –/– trained ( n = 10) mice. Protein expression determined by western blot using antibodies directed against Ca v 1.2 (B) and Ca v 1.3 (C) in individual sinus node biopsies isolated from sedentary WT ( n = 6), trained WT ( n = 4) sedentary Girk4 –/– mice ( n = 6) and trained Girk4 –/– mice ( n = 6). Representative western blots with corresponding stain-free total protein blot used for quantification shown in lower panel. Statistics: Students t -test. * p < 0.05.

    Journal: Frontiers in Physiology

    Article Title: Genetic Ablation of G Protein-Gated Inwardly Rectifying K + Channels Prevents Training-Induced Sinus Bradycardia

    doi: 10.3389/fphys.2020.519382

    Figure Lengend Snippet: mRNA expression of L-type calcium channel subunits Ca v 1.2, Ca v 1.3, Ca v 3.1, and Ca v 3.2 (A) (normalized to expression of Tbp) in SAN biopsies from from WT sedentary ( n = 10), WT trained ( n = 10), Girk4 –/– sedentary ( n = 9) and Girk4 –/– trained ( n = 10) mice. Protein expression determined by western blot using antibodies directed against Ca v 1.2 (B) and Ca v 1.3 (C) in individual sinus node biopsies isolated from sedentary WT ( n = 6), trained WT ( n = 4) sedentary Girk4 –/– mice ( n = 6) and trained Girk4 –/– mice ( n = 6). Representative western blots with corresponding stain-free total protein blot used for quantification shown in lower panel. Statistics: Students t -test. * p < 0.05.

    Article Snippet: Rabbit polyclonal anti-HCN4 (Alomone Labs, APC-052, Lot #APC052AN2802), anti-Ca v 1.2 (ACC-003, Lot #ACC003AN6802), anti-GIRK1 (APC-005, Lot #APC005AN1125) and anti-GIRK4 (APC-027, Lot #APC027AN0725), were used at 1:200. anti-Ca v 1.3 ( ) was used at 1/2000.

    Techniques: Expressing, Western Blot, Isolation, Staining

    Expression of Ca v 3.1-3.3 isoforms (Alomone) in SDH and DRG of SD rats with or without AR pre-treatment. Representative confocal images of Ca v 3.1 (A), Ca v 3.2 (B), and Ca v 3.3 (C) in SDH without AR pre-treatment (No AR). Representative confocal images of Ca v 3.1 (D), Ca v 3.2 (F), and Ca v 3.3 (F) in SDH with AR pre-treatment (AR). Representative images of Ca v 3.1 (G), Ca v 3.2 (H), and Ca v 3.3 (I) in DRG without AR pre-treatment (No AR). Representative images of Ca v 3.1 (J), Ca v 3.2 (K), and Ca v 3.3 (L) in DRG with AR pre-treatment (AR).

    Journal: European Journal of Histochemistry : EJH

    Article Title: Antigen retrieval pre-treatment causes a different expression pattern of Cav3.2 in rat and mouse spinal dorsal horn

    doi: 10.4081/ejh.2019.2988

    Figure Lengend Snippet: Expression of Ca v 3.1-3.3 isoforms (Alomone) in SDH and DRG of SD rats with or without AR pre-treatment. Representative confocal images of Ca v 3.1 (A), Ca v 3.2 (B), and Ca v 3.3 (C) in SDH without AR pre-treatment (No AR). Representative confocal images of Ca v 3.1 (D), Ca v 3.2 (F), and Ca v 3.3 (F) in SDH with AR pre-treatment (AR). Representative images of Ca v 3.1 (G), Ca v 3.2 (H), and Ca v 3.3 (I) in DRG without AR pre-treatment (No AR). Representative images of Ca v 3.1 (J), Ca v 3.2 (K), and Ca v 3.3 (L) in DRG with AR pre-treatment (AR).

    Article Snippet: Anti-Ca v 3.1-Ca v 3.3 antibodies used in the above study were all from the Alomone Company.

    Techniques: Expressing

    Expression of Ca v 3.1-3.3 isoforms (Alomone) in mice SDH sections pre-treated with AR or not. Representative images of Ca v 3.1 (A), Ca v 3.2 (B), and Ca v 3.3 (C) in SDH without AR pre-treatment (no AR). Representative images of Ca v 3.1 (D), Ca v 3.2 (E), and Ca v 3.3 (F) in SDH with AR pre-treatment (AR).

    Journal: European Journal of Histochemistry : EJH

    Article Title: Antigen retrieval pre-treatment causes a different expression pattern of Cav3.2 in rat and mouse spinal dorsal horn

    doi: 10.4081/ejh.2019.2988

    Figure Lengend Snippet: Expression of Ca v 3.1-3.3 isoforms (Alomone) in mice SDH sections pre-treated with AR or not. Representative images of Ca v 3.1 (A), Ca v 3.2 (B), and Ca v 3.3 (C) in SDH without AR pre-treatment (no AR). Representative images of Ca v 3.1 (D), Ca v 3.2 (E), and Ca v 3.3 (F) in SDH with AR pre-treatment (AR).

    Article Snippet: Anti-Ca v 3.1-Ca v 3.3 antibodies used in the above study were all from the Alomone Company.

    Techniques: Expressing